270 research outputs found
Mutation of the N-terminal proline 9 of BLMA from Streptomyces verticillus abolishes the binding affinity for bleomycin
AbstractA gene, blmA, from bleomycin (Bm)-producing Streptomyces verticillus, encodes a Bm-binding protein, designated BLMA. The expression of BLMA conferred resistance to Bm in the Escherichia coli host, whereas a mutant protein, designated Pro-9/Leu, with the N-terminal proline 9 residue in BLMA replaced by leucine, did not. We created a fusion protein between the maltose-binding protein (MBP) and a mutant protein Pro-9/Leu/Leu with Met-94 in Pro-9/Leu replaced by leucine. Pro-9/Leu/Leu from the fusion protein, obtained by digestion with CNBr digestion, did not inhibit DNA-cleaving and antibacterial activities of Bm. Native-polyacrylamide gel electrophoresis (PAGE) and gel filtration column chromatographic analysis showed that the molecular size of Pro-9/Leu/Leu is roughly half of that of BLMA, suggesting that the mutant protein cannot form dimeric structure. Furthermore, Far-UV circular dichroism (CD) spectrum of Pro-9/Leu/Leu was quite different from that of BLMA and similar to the spectra obtained from unordered proteins [Venyaminov, S.Y. and Vassilenko, K.S. (1994) Anal. Biochem. 222, 176–184], suggesting that the secondary structure of Pro-9/Leu/Leu is disrupted. These results indicate that the mutation abolishes not only dimer formation but also the secondary structure of BLMA, which results in the loss of its function as a Bm-resistance determinant
Scalability of spin FPGA: A Reconfigurable Architecture based on spin MOSFET
Scalability of Field Programmable Gate Array (FPGA) using spin MOSFET (spin
FPGA) with magnetocurrent (MC) ratio in the range of 100% to 1000% is discussed
for the first time. Area and speed of million-gate spin FPGA are numerically
benchmarked with CMOS FPGA for 22nm, 32nm and 45nm technologies including 20%
transistor size variation. We show that area is reduced and speed is increased
in spin FPGA owing to the nonvolatile memory function of spin MOSFET.Comment: 3 pages, 7 figure
Locating earthquakes around Antarctica by using neural networks based on deep learning
The Tenth Symposium on Polar Science/Ordinary sessions: [OG] Polar Geosciences, Wed. 4 Dec. / Entrance Hall (1st floor), National Institute of Polar Researc
Generalized Reciprocity Relations in Solar Cells with Voltage-Dependent Carrier Collection: Application to p-i-n Junction Devices
Two reciprocity theorems are important for fundamental understanding of the
solar cell operation and applications to device evaluation: (1) the
carrier-transport reciprocity connecting the dark-carrier injection with the
short-circuit photocarrier collection and (2) the optoelectronic reciprocity
connecting the electroluminescence with the photovoltaic quantum efficiency at
short circuit. These theorems, however, fail in devices with thick depletion
regions such as p-i-n junction solar cells. By properly linearizing the
carrier-transport equation in such devices, we report that the dark-carrier
injection is related to the photocarrier collection efficiency at the operating
voltage, not at short circuit as suggested in the original theorem. This leads
to the general form of the optoelectronic reciprocity relation connecting the
electroluminescence with the voltage-dependent quantum efficiency, providing a
correct interpretation of the optoelectronic properties of p-i-n junction
devices. We also discuss the validity of the well-known relation between the
open-circuit voltage and the external luminescence efficiency. The impact of
illumination intensity and device parameters on the validity of the reciprocity
theorems is quantitatively investigated
Clinical Aspects of IgG4-Related Orbital Inflammation in a Case Series of Ocular Adnexal Lymphoproliferative Disorders
The most frequent ocular adnexal tumors and simulating lesions are lymphoproliferative disorders (LPDs), including malignant lymphomas and orbital inflammation with lymphoid hyperplasia or infiltration. IgG4-related orbital inflammation (IgG4-ROI) often involves lacrimal glands and other orbital tissues and is an important differential diagnosis. The present study evaluated clinical aspects of IgG4-ROI in a case series of orbital LPD. Sixty-two consecutive cases of orbital LPD, pathologically diagnosed from November, 2004, through March, 2011, were investigated. Histological types were 22 cases with MALT lymphoma, 11 cases with diffuse large B-cell lymphoma (DLBCL), 3 cases with other malignant lymphomas, 16 cases with IgG4-ROI, and 10 cases with non-IgG4-ROI. Ages of the IgG4-ROI group (56 ± 10 yrs) were significantly lower than the MALT lymphoma (71 ± 12 yrs) and DLBCL (75 ± 14 yrs) groups. Orbital lesions other than lacrimal glands were present in six cases including extraocular muscle swelling, mass lesions surrounding the optic nerve, and supraorbital and infraorbital nerves enlargements. Although none of the malignant lymphomas were related to IgG4, previous evidence suggested that malignant lymphomas can arise from IgG4-ROI. Based on this study (26%) and another report (33%), it is likely that nearly a quarter of orbital LPD are IgG4-ROI
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