The Relationship Among Potential of Natural Resources, Social, Economic and Culture of Communities Inbufferzone of Mount Halimun National Park

This study was conducted to know of potential of natural resources of Mt.Halimun used by surrounding communities and the development of bufferzone.The potential of natural resources are composed of wildlife, plant biodiversity,land and hydrology.Then, The utilization of natural resources will be correlated with the condition of socio economic and culture of communities.According to the study, it is known that the major occupation of the village communities are as farmers, who have private land or as laborers (>50%). As laborers, they worked for landlord with salary Rp. 5,000 to Rp 10,000 per day for six days per week. This condition made village communities to depend their livelihood to potential of natural resources of Mt. Halimun NP. Based on dynamic hypothesis,the bufferzone could be developed through data and information of the natural resources that utilized or disturbed by communities. The plant biodiversity are used as energy, traditional medicines, food, handicrafts and forages.Illegal hunting done by surrounding communities was hunting wild pigs, birds and monkeys.To limit the exploitation, illegal cutting, illegal hunting and deforestation The Mt Halimun NP have program social forestry and agroforestry to decrease dependency of communities to the forest. For example the sheep breeding with forage plants in bufferzone.Other programs such as goats breeding and fish or plants development could be done in border land of national park or private land of communities

Ketidaklaziman Kolokasi Pembelajar Bipa dan Implikasinya terhadap Pembelajaran Bahasa

Unacceptable Collocations by Learners of Indonesian as a ForeignLanguage and the Implication in Language Learning. Foreign language learners\u27ability to collocate words that are natural and acceptable in the target language isimportant in foreign language learning; however, it is notoriously difficult forforeign language learners and sometimes makes them frustrated. This studyattempts to describe the negative transfer of English collocations into Indonesiancollocations made by learners of Indonesian as a foreign language in their writingassignments. This study employed a qualitative descriptive method. The data werecollected from 36 writing assignments by 12 learners whose mother tongue isEnglish. They were trainee teachers with experience in teaching Indonesian inAustralia. The finding shows that there are 176 unnatural Indonesian collocations,some of which are negative transfers of learners\u27 mother tongue. This suggests thatdirect teaching of collocations should be given special emphasis in teachingIndonesian as a foreign language

Control and Data Flow Execution of Java Programs

Since decade understanding of programs has become a compulsory task for the students as well as for others who are involved in the process of developing software and providing solutions to open problems. In that aspect showing the problem in a pictorial presentation in a best manner is a key advantage to better understand it. We provide model and structure for Java programs to understand the control and data flow analysis of execution. Especially it helps to understand the static analysis of Java programs, which is an uttermost important phase for software maintenance. We provided information and model for visualization of Java programs that may help better understanding of programs for a learning and analysis purpose. The idea provided for building visualization tool is extracting data and control analysis from execution of Java programs. We presented case studies to prove that our idea is most important for better understanding of Java programs which may help towards static analysis, software debugging and software maintenance

High-rise Office Tower precedents data-73 cases

As precedents study, 73 towers with a single central core were selected. Data, including building height, number of floors, standard floor area, and core area, were collected to estimate the mathematical correlations among various parameters and to generate thermal zoning for latter performance simulations.</p

Arabidopsis phosphatase under-producer mutants <i>pup1</i> and <i>pup3</i> contain mutations in the <i>AtPAP10</i> and <i>AtPAP26</i> genes

<div><p>Production and secretion of acid phosphatases (APases) is a hallmark adaptive response of plants to phosphate (Pi) deprivation. Researchers have long hypothesized that Pi starvation-induced APases are involved in internal Pi recycling and remobilization as well as in external Pi utilization. Two phosphatase under-producer (<i>pup</i>) mutants, <i>pup1</i> and <i>pup3</i>, were previously isolated in <i>Arabidopsis</i>. Characterization of these 2 <i>pup</i> mutants provided the first genetic evidence for the above hypothesis. To date, however, the molecular lesions in these 2 <i>pup</i> mutants remain unknown. In this work, we demonstrate that <i>pup1</i> and <i>pup3</i> contain point mutations in the <i>Arabidopsis</i> purple acid phosphatase gene <i>AtPAP10</i> and <i>AtPAP26</i>, respectively. Our results answer a long-standing question about the molecular identity of the <i>PUP1</i> and <i>PUP3</i> genes and corroborate the conclusions from previous studies regarding the function of AtPAP10 and AtPAP26 in plant acclimation to Pi deprivation.</p></div

Visualizing a subset of the form-performance coupled dataset.pdf

A visualized subset of the "Form-Performance" coupled dataset for high-rise office towers in north China. The simulation adopts Beijing's EPW weather file.</p

HOT_GDF API Components Index

API Components Index of HOT_GDF for grasshopper plugin. High-rise Office Tower Generative Design Framework (HOT_GDF) is a performance-based generative design framework based on VPGG, a design grammar for high-rise office towers during early design stage. </p

A Hybrid Chalcone Combining the Trimethoxyphenyl and Isatinyl Groups Targets Multiple Oncogenic Proteins and Pathways in Hepatocellular Carcinoma Cells

<div><p>Small molecule inhibitors that can simultaneously inhibit multiple oncogenic proteins in essential pathways are promising therapeutic chemicals for hepatocellular carcinoma (HCC). To combine the anticancer effects of combretastatins, chalcones and isatins, we synthesized a novel hybrid molecule 3’,4’,5’-trimethoxy-5-chloro-isatinylchalcone (3MCIC). 3MCIC inhibited proliferation of cultured HepG2 cells, causing rounding-up of the cells and massive vacuole accumulation in the cytoplasm. Paxillin and focal adhesion plaques were downregulated by 3MCIC. Surprisingly, unlike the microtubule (MT)-targeting agent CA-4 that inhibits tubulin polymerization, 3MCIC stabilized tubulin polymers both in living cells and in cell lysates. 3MCIC treatment reduced cyclin B1, CDK1, p-CDK1/2, and Rb, but increased p53 and p21. Moreover, 3MCIC caused GSK3β degradation by promoting GSK3β-Ser9 phosphorylation. Nevertheless, 3MCIC inhibited the Wnt/β-catenin pathway by downregulating β-catenin, c-Myc, cyclin D1 and E2F1. 3MCIC treatment not only activated the caspase-3-dependent apoptotic pathway, but also caused massive autophagy evidenced by rapid and drastic changes of LC3 and p62. 3MCIC also promoted cleavage and maturation of the lysosomal protease cathepsin D. Using ligand-affinity chromatography (LAC), target proteins captured onto the Sephacryl S1000-C₁₂-3MCIC resins were isolated and analyzed by mass spectrometry (MS). Some of the LAC-MS identified targets, i.e., septin-2, vimentin, pan-cytokeratin, nucleolin, EF1α1/2, EBP1 (PA2G4), cyclin B1 and GSK3β, were further detected by Western blotting. Moreover, both septin-2 and HIF-1α decreased drastically in 3MCIC-treated HepG2 cells. Our data suggest that 3MCIC is a promising anticancer lead compound with novel targeting mechanisms, and also demonstrate the efficiency of LAC-MS based target identification in anticancer drug development.</p></div

Influence of tubulin polymerization by 3MCIC.

<p>HepG2 cells were incubated at 37°C with 3MCIC, paclitaxel or colchicine, and assayed with α-tubulin mAb by immunofluorescence <b>(A)</b> and Western blotting <b>(B)</b> after separation of soluble (supernatant, S) and polymerized (pellet, P) tubulins. <b>(C)</b> HepG2 cell lysates were incubated at 25°C for 10 or 30 min with 3MCIC or paclitaxel respectively, and analyzed by Western blotting. In both <b>B</b> & <b>C</b>, normalized changes of the band-intensity ratios of P/S-tubulin in drug-treated groups over controls were plotted. *P<0.05, **P<0.01 and ***P<0.001 vs control (n = 3).</p

Diminish of paxillin and focal adhesion plaques in 3MCIC-treated HepG2 cells.

<p><b>(A)</b> Cells were treated with 3MCIC and assayed by Western blotting. Calnexin was used as loading control. <b>(B)</b> Immunofluorescence of HepG2 cells stained with paxillin mAb (green), phalloidin (red) and Hoechst 33342, after incubation with 8 or 12 μg/ml 3MCIC for 3 h.</p