Transgenic expression of phytase and acid phosphatase genes in alfalfa (Medicago sativa) leads to improved phosphate uptake in natural soils

Alfalfa (Medicagosativa L.) is one of the most widely grown crops in the USA. Phosphate (P) deficiency is common in areas where forage crops are grown. To improve the use of organic phosphate by alfalfa, two Medicagotruncatula genes, phytase (MtPHY1) and purple acid phosphatase (MtPAP1), were overexpressed in alfalfa under the control of the constitutive CaMV35S promoter or the root-specific MtPT1 promoter. Root enzyme activity analyses revealed that although both genes lead to similar levels of acid phosphatase activities, overexpression of the MtPHY1 gene usually results in a higher level of phytase activity than overexpression of the MtPAP1 gene. The MtPT1 promoter was more effective than the CaMV35S promoter in regulating gene expression and extracellular secretion under P-deficient conditions. Measurement of growth performance of the transgenic lines further proved that the best promoter–gene combination is the MtPHY1 gene driven by the MtPT1 promoter. Compared to the control, the plants with high levels of transgene expression showed improved growth. The biomass of several transgenic lines was three times that of the control when plants were grown in sand supplied with phytate as the sole P source. When the plants were grown in natural soils without additional P supplement, the best performing transgenic lines produced double the amount of biomass after 12 weeks (two cuts) of growth. Transgene effects were more obvious in soil with lower pH and lower natural P reserves than in soil with neutral pH and relatively higher P storage. The total P concentration in leaf tissues of the high-expressing transgenic lines was significantly higher than that of the control. The transgenes have great potential for improving plant P acquisition and biomass yield in P-deficient agricultural soils. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (doi:10.1007/s11032-011-9628-0) contains supplementary material, which is available to authorized users

Multiple relationships between aerosol and COVID-19: a framework for global studies

COVID-19 (Corona Virus Disease 2019) is a severe respiratory syndrome currently causing a human global pandemic. The original virus, along with newer variants, is highly transmissible. Aerosol is a multiphase system consisting of the atmosphere with suspended solid and liquid particles, which can carry toxic and harmful substances; especially the liquid components. The degree to which aerosol can carry the virus and cause COVID-19 disease is of significant research importance. In this study, we have discussed the aerosol transmission as the pathway of SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2), and the aerosol pollution reduction as a consequence of the COVID-19 lockdown. The aerosol transmission routes of the SARS-CoV-2 can be further subdivided into proximal human-exhaled aerosol transmission and potentially more distal ambient aerosol transmission. The human-exhaled aerosol transmission is a direct dispersion of the SARS-CoV-2. The ambient aerosol transmission is an indirect dispersion of the SARS-CoV-2 in which the aerosol act as a carrier to spread the virus. This indirect dispersion can also stimulate the up-regulation of the expression of SARS-CoV-2 receptor ACE-2 (Angiotensin Converting Enzyme 2) and protease TMPRSS2 (Transmembrane Serine Protease 2), thereby increasing the incidence and mortality of COVID-19. From the aerosol quality data around the world, it can be seen that often atmospheric pollution has significantly decreased due to factors such as the reduction of traffic, industry, cooking and coal-burning emissions during the COVID-19 lockdown. The airborne transmission potential of SARS-CoV-2, the infectivity of the virus in ambient aerosols, and the reduction of aerosol pollution levels due to the lockdowns are crucial research subjects

Multiple relationships between aerosol and COVID-19: A framework for global studies

COVID-19 (Corona Virus Disease 2019) is a severe respiratory syndrome currently causing a human global pandemic. The original virus, along with newer variants, is highly transmissible. Aerosols are a multiphase system consisting of the atmosphere with suspended solid and liquid particles, which can carry toxic and harmful substances; especially the liquid components. The degree to which aerosols can carry the virus and cause COVID-19 disease is of significant research importance. In this study, we have discussed aerosol transmission as the pathway of SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2), and the aerosol pollution reduction as a consequence of the COVID-19 lockdown. The aerosol transmission routes of the SARS-CoV-2 can be further subdivided into proximal human-exhaled aerosol transmission and potentially more distal ambient aerosol transmission. The human-exhaled aerosol transmission is a direct dispersion of the SARS-CoV-2. The ambient aerosol transmission is an indirect dispersion of the SARS-CoV-2 in which the aerosol acts as a carrier to spread the virus. This indirect dispersion can also stimulate the up-regulation of the expression of SARS-CoV-2 receptor ACE-2 (Angiotensin Converting Enzyme 2) and protease TMPRSS2 (Transmembrane Serine Protease 2), thereby increasing the incidence and mortality of COVID-19. From the aerosol quality data around the World, it can be seen that often atmospheric pollution has significantly decreased due to factors such as the reduction of traffic, industry, cooking and coal-burning emissions during the COVID-19 lockdown. The airborne transmission potential of SARS-CoV-2, the infectivity of the virus in ambient aerosols, and the reduction of aerosol pollution levels due to the lockdowns are crucial research subjects

COVID-19 mortality and exposure to airborne PM2.5: A lag time correlation

COVID-19 has escalated into one of the most serious crises in the 21st Century. Given the rapid spread of SARS-CoV-2 and its high mortality rate, here we investigate the impact and relationship of airborne PM2.5 to COVID-19 mortality. Previous studies have indicated that PM2.5 has a positive relationship with the spread of COVID-19. To gain insights into the delayed effect of PM2.5 concentration (μgm−3) on mortality, we focused on the role of PM2.5 in Wuhan City in China and COVID-19 during the period December 27, 2019 to April 7, 2020. We also considered the possible impact of various meteorological factors such as temperature, precipitation, wind speed, atmospheric pressure and precipitation on pollutant levels. The results from the Pearson's correlation coefficient analyses reveal that the population exposed to higher levels of PM2.5 pollution are susceptible to COVID-19 mortality with a lag time of >18 days. By establishing a generalized additive model, the delayed effect of PM2.5 on the death toll of COVID-19 was verified. A negative correction was identified between temperature and number of COVID-19 deaths, whereas atmospheric pressure exhibits a positive correlation with deaths, both with a significant lag effect. The results from our study suggest that these epidemiological relationships may contribute to the understanding of the COVID-19 pandemic and provide insights for public health strategies

Increased Formation of Follicular Antrum in Aquaporin-8-Deficient Mice Is Due to Defective Proliferation and Migration, and Not Steroidogenesis of Granulosa Cells

Aquaporin-8 (AQP8) is a water channel protein expressed exclusively in granulosa cells (GCs) in mouse ovary. Our previous studies of AQP8-deficient (AQP8-/-) mice demonstrated that AQP8 participates in folliculogenesis, including in the formation of follicles, ovulation, and atresia. However, its physiological function in formation of the antral follicle is still largely unknown. In the present study, we observed significantly increased numbers of antral follicles in AQP8-/- ovaries as well as significantly increased follicular antrum formation in in vitro 3D culture of AQP8-/- follicles. Functional detection of AQP8-/- GCs indicated that cell proliferation is impaired with FSH treatment, and wound healing and Transwell migration are also impaired with or without FSH treatment, compared with that in WT. However, the biosynthesis of estradiol and progesterone as well as the mRNA levels of key steroidogenic enzyme genes (CYP19A1 and StAR) in AQP8-/- GCs did not change, even with addition of FSH and/or testosterone. In order to estimate the influence of the impaired proliferation and migration on the density of GC mass, preantral follicles were injected with FITC-dextran, which distributes only in the intercellular space, and analyzed by confocal microscopy. The micrographs showed significantly higher transmission of fluorescence in AQP8-/- follicles, suggesting increased intercellular space of GCs. Based on this evidence, we concluded that AQP8 deficiency leads to increased formation of follicular antra in vivo and in vitro, and the mechanism may be associated with increased intercellular space of GCs, which may be caused by defective proliferation and migration of GCs. This study may offer new insight into the molecular mechanisms of the formation of follicular antrum

Emission Characteristics of NOx and SO2 during the Combustion of Antibiotic Mycelial Residue

The antibiotic mycelial residue (AMR) generated from cephalosporin C production is a hazardous organic waste, which is usually disposed of by landfilling that causes potential secondary environmental pollution. AMR combustion can be an effective method to treat AMR. In order to develop clean combustion technologies for safe disposal and energy recovery from various AMRs, the emission characteristics of NOx and SO2 from AMR combustion were studied experimentally in this work. It was found that the fuel-N is constituted by 85% protein nitrogen and 15% inorganic nitrogen, and the fuel-S by 78% inorganic sulfur and 22% organic sulfur. Nitrogen oxide emissions mainly occur at the volatile combustion stage when the temperature rises to 400 °C, while the primary sulfur oxide emission appears at the char combustion stage above 400 °C. Increasing the combustion temperature and airflow cause higher NOx emissions. High moisture content in AMR can significantly reduce the NOx emission by lowering the combustion temperature and generating more reducing gases such as CO. For the SO2 emission, the combustion temperature (700 to 900 °C), airflow and AMR water content do not seem to exhibit obvious effects. The presence of CaO significantly inhibits SO2 emission, especially for the SO2 produced during the AMR char combustion because of the good control effect on the direct emission of inorganic SO2. Employing air/fuel staging technologies in combination with in-situ desulfurization by calcium oxide/salts added in the combustor with operation temperatures lower than 900 °C should be a potential technology for the clean disposal of AMRs

Gaseous emission and ash characteristics from combustion of high ash content antibiotic mycelial residue in fluidized bed and the impact of additional water vapor

The gas (mainly NO and SO2) emission and ash characteristics from combustion of high ash content antibiotic mycelial residue (AMR) rich in N and S were investigated using a laboratory scale fluidized bed combustor, and the effects of addition of water vapor studied at the same time. The tested combustion temperature, excess air ratio (alpha) and mass ratios of water vapor-to-fuel (WV/F) varied in 750-950 degrees C, 1.3-1.9 and 0.4-1.4, respectively. Without additional water vapor present, both the concentrations of NO and SO2 in flue gas increased with elevating combustion temperature and excess air ratio, corresponding to the increases in conversion ratio of fuel-N to NO and emission ratio of S. The addition of water vapor facilitated excavating organic matters in the ash to result in its lower C and N contents. Additional water vapor formed partial reducing atmospheres, which were characterized by high CO and H-2 concentrations in combustion atmosphere and low valence S in the ash, to thus visibly reduce NO. The SO2 concentration became slightly lower at the presence of additional water vapor and further decreased with more water vapor addition. Additional water vapor strengthened capture of SO2 by the ash. Higher WV/F ratios led to more porous structures in ash, accountable for NO reduction by catalytic effect as well as SO2 absorption. Nevertheless, it seems less possible to reduce NO and SO2 concentrations to meet their respective emission criteria, and other measures will thus have to be taken to control their emissions when directly combusting AMR for energy recovery. (C) 2017 Elsevier Ltd. All rights reserved.</p

Unconstrained Optimization and Calibration of a Kinematic-Cyclic Plasticity Model

In this paper, a mixed kinematic and isotropic cyclic plasticity model based on the concept of Fuzzy Set Plasticity, which is a versatile technique for modeling a range of complex phenomena observed in non-proportional loading in soil-structure interaction, is implemented. It is followed by a discussion of associated model parameters and model parameter sensitivity analysis. The use of zero-order numerical optimization techniques is discussed and its application in calibrating the Fuzzy Set Plasticity Model is presented. The performance and comparison among these numerical optimization schemes are also examined and discussed

Fluidized bed combustion in steam-rich atmospheres for high-nitrogen fuel: Nitrogen distribution in char and volatile and their contributions to NOx

This article is devoted to investigating the contributions of char-N and volatile-N to NOx formation from fluidized bed combustion of high-nitrogen fuel (distilled spirited lees (DSL)) in steam-rich atmospheres, to make clear the effect of steam on NOx reduction and the related mechanisms. The char and the volatile prepared by pyrolysis of the DSL were subjected to combustion tests in a fluidized bed to compare their NOx emission characteristics with that from combustion of the raw DSL, respectively. The results showed that with additional steam present in pyrolysis atmosphere, significantly more gas products were formed and bigger proportions of the total fuel nitrogen entered in the gas products. Additional steam also boosted generation of aliphatic hydrocarbons containing most of the nitrogen in the tar, which was helpful for reducing NOx. The presence of additional steam accelerated the release of the volatile and remarkably increased the yield of reducing pyrolysis gases and meanwhile changed the peak release sequence of H-2, to facilitate NOx reduction. With increasing the mass ratio of steam to fuel (S/F), the total NOx from the DSL combustion at 900 degrees C in 25 vol.% O-2 atmosphere and the conversion ratio of the char-N into NOx decreased but the contribution ratio of the char-N to the total NOx increased. When the S/F ratio exceeded 0.8 the char-N contributed more to the total NOx than the volatile-N did. (C) 2016 Elsevier Ltd. All rights reserved.</p