The Teaching of Singing in Meiji Period : Mainly on the contents of teaching

Additional file 3: Fig. S4. Spontaneous gp120 shedding from cell surface. The susceptibility of gp41 mutants to spontaneously shed gp120 was determined by flow cytometry and ELISA as described previously [79]. Briefly, culture medium of transiently transfected envelope expressing cells was exchanged for fresh medium containing Brefeldin A (BioLegend) and 0.2 % Sodium azide. Cells were then incubated for 15 h at 37˚C, 5 % CO2. (a) Level of envelope expression before and after incubation was compared by staining with 2G12. (b) Amount of gp120 released during incubation was determined by gp120 capture ELISA. As a positive control, cells expressing WT envelope was incubated with 20 µg/ml sCD4, which trigger gp120 shedding. Cells expressing SIV Env (SIV) and no Env (No Env) were used as negative control. The results are shown as the means ± standard errors of four replicas

MOESM3 of Enhanced antibody-mediated neutralization of HIV-1 variants that are resistant to fusion inhibitors

Additional file 3: Fig. S3. Reactivity of antibodies, VRC01, 42F9, 49G2, b12, KD247, 16G6, 0.5Îł and 2G12 to WT and mutant envelopes. Reactivity of antibodies to envelopes was analyzed using flow cytometer, and shown as MFI. Cells expressing Env from SIVsmE543-3 was used as a negative control (SIV)