1 research outputs found
A Limonoid Kihadanin B from Immature <i>Citrus unshiu</i> Peels Suppresses Adipogenesis through Repression of the Akt-FOXO1-PPARγ Axis in Adipocytes
<i>Citrus</i> limonoids
are secondary metabolites and
exhibit a variety of biological activities. In this study, we elucidated
the suppression of adipogenesis by a <i>Citrus</i> limonoid
kihadanin B and determined its molecular mechanism in mouse 3T3-L1
adipocytes. Kihadanin B was purified from the peels of immature <i>Citrus unshiu</i> by HPLC, and its chemical structure was determined
by NMR and mass spectrometry. Kihadanin B reduced the lipid accumulation
with the reduction of the expression levels of the adipogenic and
lipogenic genes, but did not affect lipolysis in adipocytes. Phosphorylation
levels of Akt and a forkhead transcriptional factor, FOXO1, a repressor
of PPARγ, were lowered by kihadanin B. Furthermore, kihadanin
B increased the binding level of FOXO1 to the PPARγ gene promoter
in adipocytes. These results indicate that a <i>Citrus</i> limonoid kihadanin B repressed the adipogenesis by decreasing lipid
accumulation through the suppression of the Akt-FOXO1-PPARγ
axis in 3T3-L1 adipocytes