Role of CD56 in Normal Kidney Development and Wilms Tumorigenesis

The cell-surface glycoprotein CD56 has three major isoforms that play important roles in cell adhesion and signaling, which may promote cell proliferation, differentiation, survival, or migration. It is an important molecule in normal kidney development and acts as a key marker in Wilms tumor stem and progenitor cells. Here, we review the structural and genetic features of the CD56 glycoprotein, and summarize its roles in the normal versus diseased metanephric blastema. We discuss areas of CD56-related research that may complement or improve existing Wilms tumor treatment strategies, including the antibody-drug conjugate lorvotuzumab mertansine that binds to CD56

Outbreak of acute hepatitis C following the use of anti-hepatitis C virus--screened intravenous immunoglobulin therapy

BACKGROUND and AIMS: Hepatitis C virus (HCV) infection has been associated with intravenous (IV) immunoglobulin (Ig), and plasma donations used to prepare IV Ig are now screened to prevent transmission. Thirty-six patients from the United Kingdom received infusions from a batch of anti-HCV antibody-screened intravenous Ig (Gammagard; Baxter Healthcare Ltd., Thetford, Norfolk, England) that was associated with reports of acute hepatitis C outbreak in Europe. The aim of this study was to document the epidemiology of this outbreak. METHODS: Forty-six patients from the United Kingdom treated with Gammagard (34 exposed and 12 unexposed to the batch) returned epidemiological questionnaires. RESULTS: Eighty-two percent of the exposed patients (28 of 34) became positive for HCV RNA. Eighteen percent of the patients (6 of 34) who had infusions with this batch tested negative for HCV RNA, but 2 of the patients had abnormal liver function and subsequently seroconverted to anti-HCV antibody positive. Twenty-seven percent of the patients (9 of 34) developed jaundice, and 79% (27 of 34) had abnormal liver transferase levels. Virus isolates (n=21), including an isolate from the implicated batch, were genotype 1a and virtually identical by sequence analysis of the NS5 region, consistent with transmission from a single source. CONCLUSIONS: Hepatitis C infection can be transmitted by anti-HCV-screened IV Ig. Careful documentation of IV Ig batch numbers and regular biochemical monitoring is recommended for all IV Ig recipients

Mapping of serotype-specific, immunodominant epitopes in the NS-4 region of hepatitis C virus (HCV):use of type-specific peptides to serologically differentiate infections with HCV types 1, 2, and 3

The effect of sequence variability between different types of hepatitis C virus (HCV) on the antigenicity of the NS-4 protein was investigated by epitope mapping and by enzyme-linked immunosorbent assay with branched oligopeptides. Epitope mapping of the region between amino acid residues 1679 and 1768 in the HCV polyprotein revealed two major antigenic regions (1961 to 1708 and 1710 to 1728) that were recognized by antibody elicited upon natural infection of HCV. The antigenic regions were highly variable between variants of HCV, with only 50 to 60% amino acid sequence similarity between types 1, 2, and 3. Although limited serological cross-reactivity between HCV types was detected between peptides, particularly in the first antigenic region of NS-4, type-specific reactivity formed the principal component of the natural humoral immune response to NS-4. Type-specific antibody to particular HCV types was detected in 89% of the samples from anti-HCV-positive blood donors and correlated almost exactly with genotypic analysis of HCV sequences amplified from the samples by polymerase chain reaction. Whereas almost all blood donors appeared to be infected with a single virus type (97%), a higher proportion of samples (40%) from hemophiliacs infected from transfusion of non-heat-inactivated clotting factor contained antibody to two or even all three HCV types, providing evidence that long-term exposure may lead to multiple infection with different variants of HCV

INSTITUTIONS AND THE INTERNATIONAL DIFFUSION OF INFORMATION TECHNOLOG

The production and use of information technology (IT) in developed countries is wcll established and growing at a rapid pace. Newly industrializing countries are adopting both IT production and use as national goals. Developing countries are beginning to formulate plans to do the same. The institutional role in the international diffusion of IT is not well understood, but it is clear from the literature on innovation that the institutional role is critical. The paper makes four points. First, a traditional and fairly rigorous way of thinking about innovations - the economic perspective deriving from Schumpeter and Hicks - has been shown by studies from economic history and sociology/communications of innovation to be inadequate for explaining the dynamics of innovative change. The missing element is understanding of differential roles played by institutions. Second, among those promoting the need for institutional intervention there has been a debate about whether innovation is primarily supply-pushed or demand-pulled. The answer to the question has important institutional implications. The evidence, again mostly from economic history, shows it to be both, in iterative fashion. Thus institutions can intervene meaningfully on both sides. Third, there are two major forms of institutional intervention: influence and regulation. The possible intervention actions of institutions can be encompassed by a 2 x 2 matrix with supply-push and demand-pull on one dimension and influence and regulation on the other. Finally, if government wants to intervene, there are six classes of roles that might be pursued to affect innovation

Dissolved heavy metals and water quality in the surface waters of rivers and drainages of the West Peninsular Malaysia

The dissolved concentrations of metals (Cd, Cu, Pb, Ni, Fe and Zn), temperature, total dissolved solids, pH, dissolved oxygen, salinity and conductivity were determined in the surface waters of 24 geographical sampling sites including city and urban drainages and rivers, from the west Peninsular Malaysia, collected in January to April 2005. From these sampling sites, the ranges (min-max) of dissolved metal concentrations (mg/L) were Cd: 0.001-0.055, Cu: 0.001-0.1773, Pb: 0.001-1.523, Ni: 0.001-0.246, Fe: 0.001-35.67 and Zn: 0.0001-0.609 while for the water quality are pH: 4.96-9.81, dissolved oxygen (0.39-7.26 mg/L), total dissolved solids (0.002-10.02 mg/L), salinity (0.00-8.93 ppt), conductivity (3.33-17423 μS/cm) and temperature (27.8-35.3�). Some sites with elevated dissolved concentrations of heavy metals and poor water quality indicated the anthropogenic inputs of industrial and urban wastes. Regular monitoring of water quality in all drainage waters is recommended

Low-energy Scheduling Algorithms for Wearable Fall Pre-impact Detection System

ABSTRACT In this paper, novel low-energy static and dynamic scheduling algorithms with low computational complexities for heterogeneous multiprocessor systems are proposed. Since battery life of the system plays a critical role in wearable embedded systems, the algorithms are useful for energy consumption reduction in Body Area Network (BAN)-based wearable multiprocessor systems in healthcare applications. Our developed BAN-based fall pre-impact detection system is used in this investigation. Based on simulation results using the algorithms, it is found that the battery life can be extended up to 41.6 percent more of its normal life without the algorithms

The role of algal organic matter in the separation of algae and cyanobacteria using the novel “Posi” - Dissolved air flotation process

Algae and cyanobacteria frequently require separation from liquid media in both water treatment and algae culturing for biotechnology applications. The effectiveness of cell separation using a novel dissolved air flotation process that incorporates positively charged bubbles (PosiDAF) has recently been of interest but has been shown to be dependent on the algae or cyanobacteria species tested. Previously, it was hypothesised that algal organic matter (AOM) could be impacting the separation efficiency. Hence, this study investigates the influence of AOM on cell separation using PosiDAF, in which bubbles are modified using a commercially available cationic polyelectrolyte poly(N, N-diallyl-N,N-dimethylammonium chloride) (PDADMAC). The separation of Chlorella vulgaris CS-42/7, Mychonastes homosphaera CS-556/01 and two strains of Microcystis aeruginosa (CS-564/01 and CS-555/1), all of which have similar cell morphology but different AOM character, was investigated. By testing the cell separation in the presence and absence of AOM, it was determined that AOM enhanced cell separation for all the strains but to different extents depending on the quantity and composition of carbohydrates and proteins in the AOM. By extracting AOM from the strain for which optimal separation was observed and adding it to the others, cell separation improved from 90%. This was attributed to elevated levels of acidic carbohydrates as well as glycoprotein-carbohydrate conjugations, which in turn were related to the nature and quantity of proteins and carbohydrates present in the AOM. Therefore, it was concluded that process optimisation requires an in-depth understanding of the AOM and its components. If culturing algae for biotechnology applications, this indicates that strain selection is not only important with respect to high value product content, but also for cell separation