Rapid and accurate detection of SARS-CoV-2 using the RHAM technology

Abstract Rapid and sensitive detection of pathogens is of utmost importance in interrupting the transmission chain of infectious diseases. In recent years, this has proven to be vital during the coronavirus disease (COVID-19) global pandemic that put countless lives at risk. Numerous molecular diagnostic methods were used, including RT-PCR, NASBA, E-SDA, E-RCA, LAMP, and RPA. However, these technologies potentially require primer optimization and complex instruments. Here, we propose the RHAM (RNase Hybridization-Assisted amplification) system as a rapid, specific, and sensitive molecular diagnosis platform. Combining the LAMP and RNase HII-mediated fluorescent reporter, the RHAM system can amplify and visualize the target in one isothermal system with high sensitivity (5 × 102 copies/mL). There was no cross-reactivity with other common respiratory viruses. Analysis of clinical samples revealed the RHAM system to generate positive signals within 15 min without false positive or negative results. The present study shows that RHAM is not only an ideal platform for detecting pathogens, such as SARS-CoV-2 but can be potentially applied in POCT settings

The development characteristics of the discontinuous leader under the positive switching impulse with low rate of voltage rising

Discontinuous leader development is the most important discharge process under the application of the switching impulse voltage with the low rate of voltage rising, which is of great significance to study the external insulation characteristics of ultra-high voltage (UHV) large scale air gap. Based on the CMOS high-speed camera, a discharge test with different operating impulse voltage is carried out by constructing a comprehensive observation platform of rod-plate air gap discharge, and a clear discontinuous leader development process picture is captured. Moreover, the leader current, injection charge and leader channel unit length charge, and their characteristics of the change trend are also obtained. Further analysis based on the experimental results shows that the discontinuous leader development under the action of the impulse voltage with low rate of voltage rising has two different laws. Finally, this paper uses the thermodynamic equation, combined with the test results, the channel temperature changes in the discontinuous leader development stagnation stage were calculated. The results show that the leader channel temperature is still greater than 1500 K in the hundreds of microsecond time scales in the leader stagnation stage, and the subsequent leader can continue to develop on the original leader channel

Cavitation dose painting for focused ultrasound-induced blood-brain barrier disruption

Abstract Focused ultrasound combined with microbubble for blood-brain barrier disruption (FUS-BBBD) is a promising technique for noninvasive and localized brain drug delivery. This study demonstrates that passive cavitation imaging (PCI) is capable of predicting the location and concentration of nanoclusters delivered by FUS-BBBD. During FUS-BBBD treatment of mice, the acoustic emissions from FUS-activated microbubbles were passively detected by an ultrasound imaging system and processed offline using a frequency-domain PCI algorithm. After the FUS treatment, radiolabeled gold nanoclusters, 64Cu-AuNCs, were intravenously injected into the mice and imaged by positron emission tomography/computed tomography (PET/CT). The centers of the stable cavitation dose (SCD) maps obtained by PCI and the corresponding centers of the 64Cu-AuNCs concentration maps obtained by PET coincided within 0.3 ± 0.4 mm and 1.6 ± 1.1 mm in the transverse and axial directions of the FUS beam, respectively. The SCD maps were found to be linearly correlated with the 64Cu-AuNCs concentration maps on a pixel-by-pixel level. These findings suggest that SCD maps can spatially “paint” the delivered nanocluster concentration, a technique that we named as cavitation dose painting. This PCI-based cavitation dose painting technique in combination with FUS-BBBD opens new horizons in spatially targeted and modulated brain drug delivery