Circle by Providence: Supporting Discharge Readiness for Postpartum Mothers

https://digitalcommons.psjhealth.org/summit_all/1033/thumbnail.jp

Circle by Providence: Supporting Discharge Readiness for Postpartum Mothers

https://digitalcommons.psjhealth.org/stvincent-bootcamp/1004/thumbnail.jp

Supporting the Discharge Transition from NICU to Home

https://digitalcommons.psjhealth.org/stvincent-bootcamp/1024/thumbnail.jp

Structure of the human cytochrome c oxidase subunit Vb gene and chromosomal mapping of the coding gene and of seven pseudogenes

Subunit Vb of mammalian cytochrome c oxidase (COX; EC 1.9.3.1) is encoded by a nuclear gene and assembled with the other 12 COX subunits encoded in both mitochondrial and nuclear DNA. We have cloned the gene for human COX subunit Vb (COX5B) and determined the exon-intron structure by both hybridization analysis and DNA sequencing. The gene contains five exons and four introns; the four coding exons span a region of approximately 2.4 kb. The 5' end of the COX5B gene is GC-rich and contains many HpaII sites. Genomic Southern blot analysis of human DNA probed with the human COX Vb cDNA identified eight restriction fragments containing COX Vb-related sequences that were mapped to different chromosomes with panels of human x Chinese hamster somatic cell hybrids. Because only one of these fragments hybridized with a 210-bp probe from intron 4, we conclude that there is a single expressed gene for COX subunit Vb in the human genome. We have mapped this gene to chromosome 2, region cen-q13.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/29338/1/0000405.pd

Structure and chromosomal location of the bovine gene for the heart muscle isoform of cytochrome c oxidase subunit VIII

We have isolated the bovine COX8H gene for the heart/muscle isoform of cytochrome c oxidase (COX) subunit VIII from a library of bovine genomic DNA cloned into lambda EMBL3. Primer extension assays on bovine heart mRNA mapped the 5′ ends of COX8H transcripts to a CA dinucleotide 62-bp upstream from the ATG codon. The gene thus spans 1565-bp and comprises two exons and one large intron of 1227 bp. Exon 1 encodes the 5′ untranslated region, a 24-amino acid presequence, and the first 13 amino acids of the mature COX VIII-H protein. Exon 2 encodes the remainder of the cDNA: amino acids 14 to 46 plus the 66-bp 3′ untranslated region. The exon-intron boundaries matched the consensus splice junction sequences. Two protein polymorphisms were seen: an Ala/Val polymorphism at position-6 in the presequence and the previously noted Lys/Arg polymorphism at residue 7 of the mature protein. A Taq I polymorphism occurs in the intron. The COX8H gene was mapped by bovine x rodent somatic cell hybrid mapping panels to bovine (BTA) Chromosome (Chr) 25 with 100% concordancy. BTA25 is conserved relative to the long arm of human (HSA) Chr 11, which contains COX8, the gene for the single human COX VIII subunit that is homologous to the liver isoform.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/47018/1/335_2004_Article_BF00303255.pd

Automatic Registration of Aerial Image and Digital Map for Detection of Earthquake Damaged Areas

Abstract. In this paper, we propose an automatic detection method of the areas damaged by an earthquake. We use aerial images that are taken before and after the earthquake. To detect the damaged areas, we register these two aerial images. It is difficult to register two aerial images by using only the image information, and hence, we use digital map for registering them. First each aerial image is registered to the digital map by using the perspective transformation. Then we detect damaged areas by computing the differences between the registered images. The damaged areas can be detected automatically and correctly by our method.