Possibility of Ultra High-Energy Cosmic Rays from the Giant Flare in SGR 1806-20

On 2004 December 27, a giant flare from the soft gamma repeater 1806$-$20 was observed. The radiation mechanism of the initial peak of the flare would be controversial. In this letter we point out that very high-energy cosmic rays would be produced in the case that the flare was caused by internal shocks, as is usually considered for gamma-ray bursts. The highest energy of cosmic rays can reach $10^{19}$ eV, if the Lorentz factor of the shocks is sufficiently high. Future observations of cosmic rays will inform us about the mechanism of the giant flare.Comment: 8 pages, 2figure

High expression of a novel carnitine palmitoyltransferase I like protein in rat brown adipose tissue and heart: isolation and characterization of its cDNA clone

AbstractTo characterize energy metabolism in rat brown adipose tissue (BAT), we carried out differential screening of a cDNA library of BAT with a cDNA probe of white adipose tissue (WAT) and isolated one cDNA clone. It contained a single open reading frame of 2,316 bases which encodes a protein of 88.2 kDa. The predicted amino acid sequence showed the highest homology (62.6%) with that of rat carnitine palmitoyltransferase I (CPTI). The transcript corresponding to this cDNA was found to be abundantly expressed in BAT and heart. Therefore, the isolated clone is concluded to encode a CPTI like protein expressed in BAT and heart

Characterization of DNA polymerase β from Danio rerio by overexpression in E. coli using the in vivo/in vitro compatible pIVEX plasmid

<p>Abstract</p> <p>Background</p> <p>Eukaryotic DNA polymerase β (pol β), the polymerase thought to be responsible for DNA repair synthesis, has been extensively characterized in rats and humans. However, pol β has not been purified or enzymatically characterized from the model fish species <it>Danio rerio </it>(zebrafish). We used the <it>in vitro</it>/<it>in vivo </it>dual expression system plasmid, pIVEX, to express <it>Danio rerio </it>pol β (<it>Danio </it>pol β) for biochemical characterization.</p> <p>Results</p> <p><it>Danio </it>pol β encoded by the <it>in vitro</it>/<it>in vivo</it>-compatible pIVEX plasmid was expressed in <it>E. coli </it>BL21(DE3), BL21(DE3)pLysS, and KRX, and <it>in vitro </it>as a C-terminal His-tagged protein. <it>Danio </it>pol β expressed <it>in vitro </it>was subject to proteolysis; therefore, bacterial overexpression was used to produce the protein for kinetic analyses. KRX cells were preferred because of their reduced propensity for leaky expression of pol β. The cDNA of <it>Danio rerio </it>pol β encodes a protein of 337 amino acids, which is 2-3 amino acids longer than other pol β proteins, and contains a P63D amino acid substitution, unlike mammalian pol βs. This substitution lies in a hairpin sequence within an 8-kDa domain, likely to be important in DNA binding. We performed extensive biochemical characterization of <it>Danio </it>pol β in comparison with rat pol β, which revealed its sensitivity to metal ion activators (Mn²⁺and Mg²⁺), its optimum salt concentration (10 mM KCl and 50 mM NaCl), alkaline pH optimum (pH 9.0), and low temperature optimum (30°C). Substituting Mn²⁺for Mg²⁺resulted in 8.6-fold higher catalytic efficiency (<it>k</it>_cat/<it>K</it>_m).</p> <p>Conclusions</p> <p>Our characterization of pol β from a model fish organism contributes to the study of the function and evolution of DNA polymerases, which are emerging as important cellular targets for chemical intervention in the development of anticancer agents.</p

Gene suppression via U1 small nuclear RNA interference (U1i) machinery using oligonucleotides containing 2′-modified-4′-thionucleosides

AbstractGene suppression via U1 small nuclear RNA interference (U1i) is considered to be one of the most attractive approaches, and takes the place of general antisense, RNA interference (RNAi), and anti-micro RNA machineries. Since the U1i can be induced by short oligonucleotides (ONs), namely U1 adaptors consisting of a ‘target domain’ and a ‘U1 domain’, we prepared adaptor ONs using 2′-modified-4′-thionucleosides developed by our group, and evaluated their U1i activity. As a result, the desired gene suppression via U1i was observed in ONs prepared as a combination of 2′-fluoro-4′-thionucleoside and 2′-fluoronucleoside units as well as only 2′-fluoronucleoside units, while those prepared as combination of 2′-OMe nucleoside/2′-OMe-4′-thionucleoside and 2′-fluoronucleoside units did not show significant activity. Measurement of Tm values indicated that a higher hybridization ability of adaptor ONs with complementary RNA is one of the important factors to show potent U1i activity

S-Z power spectrum produced by primordial magnetic fields

Primordial magnetic fields generated in the very early universe are one of the candidates for the origin of magnetic fields observed in galaxy clusters. After recombination, the Lorentz force acts on the residual ions and electrons to generate density fluctuations of baryons. Accordingly these fluctuations induce the early formation of dark halos which cause the Sunyaev-Zel'dovich (S-Z) effect in cosmic microwave background radiation. This additional S-Z effect due to primordial magnetic fields amplifies the angular power spectrum of cosmic microwave temperature anisotropies on small scales. This amplification depends on the comoving amplitude and the power law index of the primordial magnetic fields spectrum. Comparing with the small scale CMB observations, we obtained the constraints on the primordial magnetic fields, i.e., B < 2.0 nGauss for n=-2.9 or B < 1.0 nGauss for n=-2.6, where B is the comoving amplitude of magnetic fields at h^-1 Mpc and n is the power law index. Future S-Z measurements have the potential to give constraints tighter than those from temperature anisotropies and polarization of cosmic microwave background induced by the magnetic fields at the recombination epoch.Comment: 9 pages, 6 figures. accepted by MNRA

Identification of possible protein machinery involved in the thermogenic function of brown adipose tissue

Brown adipose tissue (BAT) is believed to function by dissipating excess energy in mammals. It is very important to understand the energy metabolism held in BAT since disorder of its energy-dissipating function may cause obesity or lifestyle-related diseases such as hypertension and diabetes. This function in BAT is mainly attributable to uncoupling protein (UCP), specifically expressed in its mitochondria. This protein consumes excess energy as heat by dissipating the H+ gradient across the inner mitochondrial membrane that is utilized as a driving force for ATP synthesis. In this review article, in addition to providing a brief introduction to the functional properties of BAT and UCP, we also describe and discuss properties of cultured brown adipocytes and the results of our exploratory studies on protein components involved in the energy-dissipating function in BAT

Search for a stochastic background of 100-MHz gravitational waves with laser interferometers

This letter reports the results of a search for a stochastic background of gravitational waves (GW) at 100 MHz by laser interferometry. We have developed a GW detector, which is a pair of 75-cm baseline synchronous recycling (resonant recycling) interferometers. Each interferometer has a strain sensitivity of ~ 10^{-16} Hz^{-1/2} at 100 MHz. By cross-correlating the outputs of the two interferometers within 1000 seconds, we found h_{100}^2 Omega_{gw} < 6 times 10^{25} to be an upper limit on the energy density spectrum of the GW background in a 2-kHz bandwidth around 100 MHz, where a flat spectrum is assumed.Comment: Accepted by Phys.Rev.Lett.; 10 pages, 4 figure

Use of modified U1 small nuclear RNA for rescue from exon 7 skipping caused by 5′-splice site mutation of human cathepsin A gene

Cathepsin A (CTSA) is a multifunctional lysosomal enzyme, and its hereditary defect causes an autosomal recessive disorder called galactosialidosis. In a certain number of galactosialidosis patients, a base substitution from adenine to guanine is observed at the +3 position of the 7th intron (IVS7 +3a>g) of the CTSA gene. With this mutation, a splicing error occurs; and consequently mRNA lacking the 7th exon is produced. This skipping of exon 7 causes a frame shift of the transcripts, resulting in a non-functional CTSA protein and hence galactosialidosis. This mutation seems to make the interaction between the 5’-splice site of intron 7 of pre-mRNA and U1 small nuclear RNA (U1 snRNA) much weaker. In the present study, to produce properly spliced mRNA from the CTSA gene harboring this IVS7 +3a>g mutation, we examined the possible usefulness of modified U1 snRNA that could interact with the mutated 5’-splice site. Toward this goal, we first prepared a model system using a mutant CTSA mini gene plasmid for delivery into HeLa cells. Then, we examined the effectiveness of modified U1 snRNA on the formation of properly spliced mRNA from this mutant CTSA mini gene. As a result, we succeeded in obtaining improved formation of properly spliced CTSA mRNA. Our results suggest the usefulness of modified U1 snRNA for rescue from exon 7 skipping caused by the IVS7 +3a>g mutation of the CTSA gene

Characteristics of unique endocytosis induced by weak current for cytoplasmic drug delivery

We previously reported that 20 a weak current (WC, 0.3-0.5mA/cm2) applied to cells can induce endocytosis to promote cytoplasmic delivery of hydrophilic macromolecules (MW: < 70,000), such as dextran and siRNA, which leak from WC-induced endosomes into the cytoplasm (Hasan et al., 2016). In this study, we evaluated the characteristics of WC-mediated endocytosis for application of the technology to cytoplasmic delivery of macromolecular medicines. WC induced significantly higher cellular uptake of exogenous DNA fragments compared to untreated cells; the amount increased in a time-dependent manner, indicating that endocytosis was induced after WC. Moreover, following WC treatment of cells in the presence of an antibody (MW: 150,000) with the lysosomotropic agent chloroquine, the antibody was able to bind to its intracellular target. Thus, high molecular weight protein medicines delivered by WC-mediated endocytosis were functional in the cytoplasm. Transmission electron microscopy of cells treated by WC in the presence of gold nanoparticles covered with polyethylene glycol showed that the WC-induced endosomes exhibited an elliptical shape. In the WC-induced endosomes, ceramide, which makes pore structures in the membrane, was localized. Together, these results suggest that WC can induce unique endocytosis and that macromolecular medicines leak from endosomes through a ceramide pore

Gene suppression via U1 small nuclear RNA interference (U1i) machinery using oligonucleotides containing 2'-modified-4'-thionucleosides

Gene suppression via U1 small nuclear RNA interference (U1i) is considered to be one of the most attractive approaches, and takes the place of general antisense, RNA interference (RNAi), and anti-micro RNA machineries. Since the U1i can be induced by short oligonucleotides (ONs), namely U1 adaptors consisting of a ‘target domain’ and a ‘U1 domain’, we prepared adaptor ONs using 2'-modified-4'-thionucleosides developed by our group, and evaluated their U1i activity. As a result, the desired gene suppression via U1i was observed in ONs prepared as a combination of 2'-fluoro-4'-thionucleoside and 2'-fluoronucleoside units as well as only 2'-fluoronucleoside units, while those prepared as combination of 2'-OMe nucleoside/2'-OMe-4'-thionucleoside and 2'-fluoronucleoside units did not show significant activity. Measurement of Tm values indicated that a higher hybridization ability of adaptor ONs with complementary RNA is one of the important factors to show potent U1i activity