Prevalência de duplicação interna in tandem/fms-receptor tirosino-quinase (DIT/FLT3) em pacientes com leucemia mielóide aguda de novo classificados conforme grupos citogenéticos de risco

CONTEXT AND OBJECTIVE: The mechanism involved in leukemogenesis remains unclear and more information about the disruption of the cell proliferation, cell differentiation and apoptosis of neoplastic cells is required. DESIGN AND SETTING: Cross-sectional prevalence study at the Discipline of Hematology, Hospital São Paulo, Universidade Federal de São Paulo (UNIFESP). METHODS: We investigated FMS-like tyrosine kinase 3/internal tandem duplication (FLT3/ITD+) in 40 adult patients with de novo acute myeloid leukemia (AML), categorized according to cytogenetic results, from September 2001 to May 2005. RESULTS: Thirteen patients (32.5%) were classified as presenting the favorable karyotype, 11 patients (27.5%) as an intermediate group, 7 patients (17%) as an undefined group and 9 patients (22.5%) as the unfavorable group. FLT3/ITD+ was found in 10 patients (25%): 3 with FLT3/ITD+ and favorable karyotype; 4 with FLT3/ITD+ and intermediate karyotype; 2 with FLT3/ITD+ and undefined karyotype; and only 1 with FLT3/ITD+ and unfavorable karyotype. Among the patients without FLT3/ITD+, 10 presented favorable karyotype, 8 intermediate, 4 undefined and 8 unfavorable karyotype. The cytogenetic results showed no correlations between FLT3/ITD presence and the prognostic groups (P = 0.13). We found that 2 patients were still alive more than 24 months later, FLT3/ITD+ did not influence the patients' survival rate. CONCLUSION: We found the same frequency of AML with FLT3/ITD+ in both the favorable and intermediate prognosis groups. Only one patient presented AML, FLT3/ITD+ and unfavorable karyotype (the hypothetical worst clinical situation). Therefore, the prognostic advantage of favorable cytogenetics among patients with FLT3/ITD+ remains to be elucidated, for it to be better understood.CONTEXTO E OBJETIVO: O mecanismo envolvido na leucemogênese permanece obscuro, e maiores informações a respeito das inadequadas proliferação, diferenciação e apoptose das células neoplásicas é fundamental. TIPO DE ESTUDO E LOCAL: Estudo transversal de prevalência na Disciplina de Hematologia e Hemoterapia, Hospital São Paulo, Universidade Federal de São Paulo (UNIFESP). MÉTODOS: Nós pesquisamos a duplicação interna in tandem (DIT) do gene FLT3 (Fms-like tyrosine kinase) em 40 pacientes adultos com leucemia mielóide aguda (LMA) de novo, classificados de acordo com os resultados de cariótipo em banda G, de setembro de2001 a maio de 2005. RESULTADOS: Treze pacientes (32,5%) foram classificados como cariótipo favorável, 11 pacientes (27,5%) como grupo intermediário, 7 pacientes (17%) no grupo de prognóstico indefinido e os restantes 9 pacientes (22,5%) foram alocados como desfavorável. A DIT/FLT3 foi encontrada em 10 pacientes (25%), 3 pacientes com DIT/FLT3 e cariótipo favorável, 4 com DIT/FLT3 e cariótipo intermediário, 2 com DIT/FLT3 e cariótipo de prognóstico indefinido e somente 1 paciente com DIT/FLT3 e cariótipo desfavorável. Entre os pacientes sem DIT/FLT3, 10 apresentaram cariótipo favorável, 8 com cariótipo intermediário, 4 com cariótipo de prognóstico indefinido e 8 com cariótipo desfavorável. Não houve correlação entre a presença de DIT/FLT3 e os grupos de prognóstico conforme resultados de citogenética (P = 0,13). No presente estudo encontramos 2 pacientes vivos por mais de 24 meses. A presença de DIT/FLT3 não influenciou a taxa de sobrevida dos pacientes. CONCLUSÃO: Nós observamos a mesma frequência de LMA com DIT/FLT3 tanto no grupo de cariótipo favorável quanto no grupo intermediário e somente um paciente com LMA e DIT/FLT3 e cariótipo desfavorável, hipoteticamente o pior achado clínico. Desta forma, a vantagem prognóstica do cariótipo favorável em pacientes DIT/FLT3 permanece a ser esclarecida para melhor compreensão da LMA

Plasma levels of FL and SDF-1 and expression of FLT-3 and CXCR4 on CD34+cells assessed pre and post hematopoietic stem cell mobilization in patients with hematologic malignancies and in healthy donors

Peripheral blood stem cells (PBSC) became the main source of cells for autologous transplantation. Alterations in the expression of adhesion molecules are essential in the CD34+ cells mobilization process. These molecules are involved in the interaction between hematopoietic and stromal cells and they have been disclosed as a considerable factor to the trafficking and homing of the CD34+ progenitor cells.This is a non-randomized PBSC mobilization study designed to evaluate the influence and behavior of FL and SDF-1 and their receptors in two different moments, prior and after HPCs mobilization, with the yield of CD34+ cells collected by apheresis.There was higher concentration of FL and lower of SDF-1 plasma level at post than pre PBSC mobilization (p = 0.001 and p m 0.012, respectively) regarding all individuals searched, but without any correlation with a good yield of CD34+ cells. However, CXCR4 expressions on the CD34+ cells from bone marrow aspirates (BMA), at pre and post mobilization showed a difference statistical significant for those individuals with good yield of CD34+ cells (p = 0,036), but not achieved for poor yield (p = 0,156). There was a higher expression of CXCR4 in steady-state for the Successfully individuals than for those unsuccessfully (529.84 +/- 54.68 and 496.31 +/- 97.51, respectively). in conclusion, we confirmed the important role of CXCR4/SDF-1 axis in the process of PBSC mobilization. (C) 2009 Elsevier B.V. All rights reserved.Universidade Federal de São Paulo, Dept Clin & Expt Pharmacol Oncol, BR-04023902 São Paulo, BrazilUniversidade Federal de São Paulo, Dept Clin & Expt Pharmacol Oncol, BR-04023902 São Paulo, BrazilWeb of ScienceFundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP

Interaction of laminin and fibronectin with leukemic cells

Universidade de São Paulo Faculdade de Medicina Departamento de Clínica Médica. Disciplina de OncologiaEscola Paulista de Medicina Servico de HematologiaUniversidade de São Paulo Faculdade de Medicina Departamento de Clinica Medica. Disciplina de ReumatologiaUniversidade de São Paulo Faculdade de Medicina Departamento de Clínica Médica. Disciplina de OncologiaUNIFESP, EPM, Servico de HematologiaSciEL

Chronic lymphocytic leukemia in Brazil: A retrospective analysis of 1903 cases

Univ Fed São Paulo, UNIFESP, EPM, São Paulo, BrazilHosp Sirio Libanes, São Paulo, BrazilUniv Estadual Campinas, Campinas, BrazilHemope, Hemoctr Pernambuco, Recife, PE, BrazilSanta Casa de Misericordia São Paulo, São Paulo, BrazilHosp Samaritano, São Paulo, BrazilHosp Brigadeiro, Hosp Transplantes Euryclides de Jesus Zerbini, São Paulo, BrazilHosp Amaral Carvalho, Jau, BrazilUniv Fed Minas Gerais, Belo Horizonte, MG, BrazilHosp Israelita Albert Einstein, São Paulo, BrazilHosp Servidor Publ Estadual, São Paulo, BrazilCasa Saude Santa Marcelina, São Paulo, BrazilUniv Fed Goias, Goiania, Go, BrazilUniv Fed Ceara, Fortaleza, Ceara, BrazilInst Canc São Paulo, São Paulo, BrazilUniv Fed Parana, Curitiba, Parana, BrazilHosp Sao Vicente de Paulo, Passo Fundo, BrazilUniv Fed São Paulo, UNIFESP, EPM, São Paulo, BrazilWeb of Scienc

Results of FLT3 mutation screening and correlations with immunophenotyping in 169 Brazilian patients with acute myeloid leukemia

Univ São Paulo, Div Hematol, Dept Internal Med,Med Sch Ribeirao Preto, Natl Inst Sci & Technol Cell Based Therapy, BR-14049 Ribeirao Preto, BrazilUniv São Paulo, Dept Genet, Natl Inst Sci & Technol Cell Based Therapy, Med Sch Ribeirao Preto, BR-14048900 Ribeirao Preto, SP, BrazilUniversidade Federal de São Paulo, Dept Hematol & Hemotherapy, São Paulo, BrazilUniversidade Federal de São Paulo, Dept Hematol & Hemotherapy, São Paulo, BrazilWeb of Scienc