Electroencapsulation of Trans-resveratrol in Nanoparticles Composed of Silk Fibroin and Soluble Eggshell Membrane Protein

WOS:000607984700001The beneficial effects of trans-resveratrol on health are widely accepted. However, when exposed to heat and UV light, the degradation of trans-resveratrol to less active form cis-resveratrol limits its use in industrial applications. Due to this reason, it is crucial to preserve the stability of trans-resveratrol by using carrier systems. This study aimed to encapsulate the trans-resveratrol in core/shell nanoparticles composed of eggshell membrane proteins and silk fibroin, respectively, using a coaxial electrospraying technique to preserve its stability. The size of the nanoparticles ranged from 8.2 to 254 nm. Keeping the encapsulation yield at the maximum level (96.9%), electroencapsulation process parameters which minimize the average particle diameter (23.8 nm) were found to be A (silk fibroin concentration) = 30.7 mg/ml, B (ratio of flow rates) = 0.72, C (applied voltage) = 18.8 kV, and D (distance) = 12.2 cm. Encapsulation efficiency varied between 40.05 and 96.41%. Detection of antioxidant capacity of released trans-resveratrol suggested that nanoparticles could be a suitable delivery system for sustained release of trans-resveratrol with preserved thermal and UV photostability. Central composite design (CCD) and the response surface methodology (RSM) were successfully used to optimize the electroencapsulation process parameters for the preparation of trans-resveratrol loaded core/shell nanoparticles. It was found that these parameters seemed to be varied depending on the response required. Therefore, an optimum process should be investigated to obtain desired responses such as high encapsulation yield, high encapsulation efficiency, and small average particle size while preserving the thermal and UV stabilities at reasonable levels.Scientific and Technological Research Council of Turkey (TUBITAK)Turkiye Bilimsel ve Teknolojik Arastirma Kurumu (TUBITAK) [116M566]This research was supported by the Scientific and Technological Research Council of Turkey (TUBITAK) under grant number 116M566

Crystal structure and photoluminescence properties of a new Cd-II coordination polymer catena-poly[bis[4-bromo-2-({[2-(pyrrolidin-1-yl)ethyl]imino}methyl)phenolato-kappa N-3,N ',O]di-mu(3)-chlorido-di-mu(2)-chlorido-bis(methanol-kappa O)tricadmium(II)]

Schiff base-metal complexes have been used widely as catalysts for many organic reactions, such as ring-opening polymerization and oxidation. In view of the importance of Cd-II coordination polymers and in an effort to enlarge the library of such complexes, the title novel polymeric Cd-II tridentate Schiff base complex, [Cd-3(C13H16BrN2O)(2)Cl-4(CH4O)(2)](n), has been synthesized and characterized by elemental analyses, UV and IR spectroscopies, and single-crystal X-ray diffraction. The complex crystallizes in the triclinic P space group with two symmetry-independent Cd-II atoms, one of which lies on an inversion centre, and analysis of the crystal structure shows that both Cd-II atoms are six-coordinated; the environment around one Cd-II atom can be described as distorted octahedral, while that around the second Cd-II atom is octahedral. The Cd-II atoms are linked by chloride ligands to form a one-dimensional coordination polymer. The nonbonding intermolecular Cd center dot center dot center dot Cd distances are 3.7009 (4) and 4.3563 (5) angstrom. Furthermore, the photoluminescence properties of the complex have been investigated and it displays a strong red emission in the solid state at room temperature

Chlorometallate-Pyridinium Boronic Acid Salts for Crystal Engineering: Synthesis of One‑, Two‑, and Three-Dimensional Hydrogen Bond Networks

A series of new crystal structures of salts containing 3- and 4-pyridineboronic acid (3- and 4-pba) with chlorometallate have been prepared: [4-pbaH]­[MnCl₂] (<b>1</b>), [4-pbaH]­[CdCl₂], (<b>2</b>), [4-pbaH]₂[CuCl₄] (<b>3</b>), [4-pbaH]₂[PdCl₄] (<b>4</b>), [3-pbaH]₂[CdCl₄] (<b>5</b>), [3-pbaH]­[CuCl₃(OH₂)] (<b>6</b>), and [3-pbaH]­[PdCl₂] (<b>7</b>). In these salts five structural forms for the chlorometallate species are observed: mononuclear square planar [M = Pd (<b>4</b> and <b>7</b>)], dimeric square-pyramidal [M = Cu (<b>3</b>)], polymeric square-pyramidal [M = Cu (<b>6</b>)], polymeric <i>trans</i>-edge-sharing octahedral [M = Mn (<b>1</b>), Cd (<b>2</b>)], and polymeric <i>cis</i>-edge-sharing octahedral [M = Cd (<b>5</b>)]. The cyclic R2,2(8) boronic acid dimer is formed in the salt of <b>1</b>–<b>5</b>. NH···Cl₂M synthons <b>A</b> have been exploited in <b>1</b>–<b>4</b>, <b>6</b>, and <b>7</b>. Both NH···Cl₂M (sythons <b>A</b>) and B­(OH)₂···Cl₂M (sythons <b>B</b>) interactions give rise to a synthon of form <b>I</b> in <b>6</b>. The uncommon folded conformation of the NH···Cl₂M (synthons <b>A</b>) are formed in <b>7</b>. The NH or OH donors and consequent branching of the NH···Cl hydrogen-bond network leads to one-, two-, and three-dimensional structures. These structures are further stabilized by CH···O and CH···Cl and π···π stacking interactions

Epidemiological, Clinical, and Laboratory Features of Children With COVID-19 in Turkey

Objectives: The aim of this study is to identify the epidemiological, clinical, and laboratory features of coronavirus disease 2019 (COVID-19) in children

Recessive inborn errors of type I IFN immunity in children with COVID-19 pneumonia

In an international cohort of 112 children hospitalized for moderate to critical COVID-19 pneumonia, we identified 12 children with one of four known recessive inborn errors of type I interferon immunity: X-linked TLR7 and autosomal IFNAR1, STAT2, and TYK2 deficiencies.Recessive or dominant inborn errors of type I interferon (IFN) immunity can underlie critical COVID-19 pneumonia in unvaccinated adults. The risk of COVID-19 pneumonia in unvaccinated children, which is much lower than in unvaccinated adults, remains unexplained. In an international cohort of 112 children (&lt;16 yr old) hospitalized for COVID-19 pneumonia, we report 12 children (10.7%) aged 1.5-13 yr with critical (7 children), severe (3), and moderate (2) pneumonia and 4 of the 15 known clinically recessive and biochemically complete inborn errors of type I IFN immunity: X-linked recessive TLR7 deficiency (7 children) and autosomal recessive IFNAR1 (1), STAT2 (1), or TYK2 (3) deficiencies. Fibroblasts deficient for IFNAR1, STAT2, or TYK2 are highly vulnerable to SARS-CoV-2. These 15 deficiencies were not found in 1,224 children and adults with benign SARS-CoV-2 infection without pneumonia (P = 1.2 x 10(-11)) and with overlapping age, sex, consanguinity, and ethnicity characteristics. Recessive complete deficiencies of type I IFN immunity may underlie similar to 10% of hospitalizations for COVID-19 pneumonia in children