Microbial diversities (16S and 18S rRNA gene pyrosequencing) and environmental pathogens within drinking water biofilms grown on the common premise plumbing materials unplasticized polyvinylchloride and copper

Drinking water (DW) biofilm communities influence the survival of opportunistic pathogens, yet knowledge about the microbial composition of DW biofilms developed on common in-premise plumbing material is limited. Utilizing 16S and 18S rRNA gene pyrosequencing, this study characterized the microbial community structure within DW biofilms established on unplasticized polyvinyl chloride (uPVC) and copper (Cu) surfaces and the impact of introducing Legionella pneumophila (Lp) and Acanthamoeba polyphaga. Mature (\u3e 1 year old) biofilms were developed before inoculation with sterilized DW (control, Con), Lp, or Lp and A. polyphaga (LpAp). Comparison of uPVC and Cu biofilms indicated significant differences between bacterial (P = 0.001) and eukaryotic (P \u3c 0.01) members attributable to the unique presence of several family taxa: Burkholderiaceae, Characeae, Epistylidae, Goniomonadaceae, Paramoebidae, Plasmodiophoridae, Plectidae, Sphenomonadidae, and Toxariaceae within uPVC biofilms; and Enterobacteriaceae, Erythrobacteraceae, Methylophilaceae, Acanthamoebidae, and Chlamydomonadaceae within Cu biofilms. Introduction of Lp alone or with A. polyphaga had no effect on bacterial community profiles (P \u3e 0.05) but did affect eukaryotic members (uPVC, P \u3c 0.01; Cu, P = 0.001). Thus, established DW biofilms host complex communities that may vary based on substratum matrix and maintain consistent bacterial communities despite introduction of Lp, an environmental pathogen

Convergence and Disparities in Higher Education Fiscal Expenditures in China: A Regional Perspective

У цьому дослідженні досліджуються відмінності та конвергенція у фінансових витратах на вищу освіту в різних регіонах Китаю. У дослідженні використовується аналіз коефіцієнтів Джині та тести σ-конвергенції/β-конвергенції для кількісної оцінки ступеня розбіжностей і вивчення тенденцій конвергенції протягом дванадцятирічного періоду дослідження (2007–2018). Результати проливають світло на дисбаланс у розподілі ресурсів і дають цінну інформацію про зусилля, необхідні для досягнення більш справедливого розподілу бюджетних ресурсів для вищої освіти. Отримані результати показують значні відмінності у фінансових витратах на вищу освіту між східним, центральним, західним і північно-східним регіонами, причому східний регіон демонструє найбільший розрив порівняно з іншими. Примітно, диспропорція між східним і центральним регіонами навіть більша, ніж між східним і західним регіонами, що підкреслює необхідність цілеспрямованих втручань для усунення регіональних дисбалансів. Протягом досліджуваного періоду розрив між Східним і Центральним регіонами залишався стабільно вищим, ніж інші регіональні відмінності. Крім того, дослідження показує загальну тенденцію до скорочення розбіжностей у фіскальних видатках у регіонах, причому найбільш виражена конвергенція спостерігається між центральним і північно-східним регіонами. У Західному регіоні спостерігаються дещо більші відмінності, ніж у Центральному та Північно-Східному регіонах, що, ймовірно, пояснюється більшою підтримкою фіскальної політики та меншою кількістю студентів. Тим не менш, розрив у фіскальних видатках між Західним і Центральним регіонами продемонстрував тенденцію до скорочення. Дослідження також досліджує абсолютну та умовну β-конвергенцію, виявляючи помітні моделі конвергенції у східному та центральному регіонах. Однак західні та північно-східні регіони демонструють різний ступінь конвергенції, що вказує на необхідність механізмів конвергенції, характерних для кожного регіону. Щоб досягти збалансованого розподілу фінансових ресурсів для вищої освіти між регіонами, дослідження рекомендує цільову фіскальну політику, додаткове фінансування та покращення прозорості та підзвітності. Політики повинні зосередитися на посиленні механізмів конвергенції для забезпечення більш справедливого розподілу ресурсів і сприяння сталому розвитку вищої освіти в усій країні. Незважаючи на те, що це дослідження дає цінну інформацію, важливо розглянути інші потенційні фактори, що впливають на диспропорції у фіскальних видатках, такі як політична орієнтація, економічні відмінності та демографічні структури, для більш повного розуміння. У майбутніх дослідженнях можуть бути використані якісні дослідження для подальшого вивчення складнощів дисбалансу фінансових витрат на вищу освіту та визначення ефективних заходів політики.This research investigates the disparities and convergence in higher education fiscal expenditures across different regions in China. The study utilises Gini coefficient analysis and σ-convergence/β-convergence tests to quantify the extent of disparities and explore convergence trends over a twelve-year investigation period (2007–2018). The results shed light on the imbalances in resource allocation and provide valuable insights into the efforts required to achieve a more equitable distribution of fiscal resources for higher education. The findings reveal significant disparities in higher education fiscal expenditures between the Eastern, Central, Western, and Northeastern regions, with the Eastern region exhibiting the largest gap compared to others. Remarkably, the disparity between the Eastern and Central regions is even greater than that between the Eastern and Western regions, emphasising the need for targeted interventions to address regional imbalances. Over the study period, the gap between the Eastern and Central regions remained consistently higher than other regional disparities. Moreover, the research shows a general trend towards narrowing regional fiscal expenditure disparities, with the most pronounced convergence observed between the Central and Northeastern regions. The Western region exhibits slightly larger disparities than the Central and Northeastern regions, possibly attributed to greater fiscal policy support and lower student enrollments. Nevertheless, the fiscal expenditure gap between the Western and Central regions has shown a trend towards reduction. The study also explores absolute and conditional β-convergence, revealing notable convergence patterns in the Eastern and Central regions. However, the Western and Northeastern regions exhibit varying degrees of convergence, indicating the necessity for region-specific convergence mechanisms. To achieve a balanced allocation of financial resources for higher education across regions, the study recommends targeted fiscal policies, additional funding, and improved transparency and accountability. Policymakers should focus on enhancing convergence mechanisms to ensure a more equitable distribution of resources and foster the sustainable development of higher education throughout the country. While this research provides valuable insights, it is essential to consider other potential factors influencing fiscal expenditure disparities, such as policy orientation, economic disparities, and demographic structures, for a more comprehensive understanding. Future research may benefit from qualitative investigations to further explore the complexities of higher education fiscal expenditure imbalances and identify effective policy interventions

On q-Gaussians and Exchangeability

The q-Gaussians are discussed from the point of view of variance mixtures of normals and exchangeability. For each q< 3, there is a q-Gaussian distribution that maximizes the Tsallis entropy under suitable constraints. This paper shows that q-Gaussian random variables can be represented as variance mixtures of normals. These variance mixtures of normals are the attractors in central limit theorems for sequences of exchangeable random variables; thereby, providing a possible model that has been extensively studied in probability theory. The formulation provided has the additional advantage of yielding process versions which are naturally q-Brownian motions. Explicit mixing distributions for q-Gaussians should facilitate applications to areas such as option pricing. The model might provide insight into the study of superstatistics.Comment: 14 page

Adaptation to climate change: a comparative analysis of modelling methods for heat-related mortality

Background: Multiple methods are employed for modelling adaptation when projecting the impact of climate change on heat-related mortality. The sensitivity of impacts to each is unknown because they have never been systematically compared. In addition, little is known on the relative sensitivity of impacts to “adaptation uncertainty” (i.e. the inclusion/exclusion of adaptation modelling), relative to using multiple climate models and emissions scenarios. Objectives: (1) Compare the range in projected impacts that arises from using different adaptation modelling methods; (2) compare the range in impacts that arises from adaptation uncertainty to ranges from using multiple climate models and emissions scenarios; (3) recommend modelling method(s) to use in future impact assessments. Methods: We estimated impacts for 2070-2099, for 14 European cities, applying six different methods for modelling adaptation; also with climate projections from five climate models, run under two emissions scenarios to explore the relative effects of climate modelling and emissions uncertainty. Results: The range of the difference (%) in impacts between including and excluding adaptation, irrespective of climate modelling and emissions uncertainty, can be as low as 28% with one method and up to 103% with another (mean across 14 cities). In 13 of 14 cities the ranges in projected impacts due to adaptation uncertainty are larger than those associated with climate modelling and emissions uncertainty. Conclusions: Researchers should carefully consider how to model adaptation because it is a source of uncertainty that can be greater than the uncertainty in emissions and climate modelling. We recommend absolute threshold shifts and reductions in slope

Control of Intestinal Inflammation, Colitis-Associated Tumorigenesis, and Macrophage Polarization by Fibrinogen-Like Protein 2

Fibrinogen-like protein 2 (Fgl2) is critical for immune regulation in the inflammatory state. Elevated Fgl2 levels are observed in patients with inflammatory bowel disease (IBD), but little is known about its functional significance. In this study, we sought to investigate the role of Fgl2 in the development of intestinal inflammation and colitis-associated colorectal cancer (CAC). Here, we report that Fgl2 deficiency increased susceptibility to dextran sodium sulfate-induced colitis and CAC in a mouse model. During colitis development, the expression of the membrane-bound and secreted forms of Fgl2 (mFgl2 and sFgl2, respectively) in the colon were increased and predominantly expressed by colonic macrophages. In addition, using bone marrow chimeric mice, we determined that Fgl2 function in colitis is strictly related to its expression in the hematopoietic cells. Loss of Fgl2 induced the polarization of M1, but suppressed that of M2 both in vivo and in vitro, independent of intestinal inflammation. Thus, Fgl2 suppresses intestinal inflammation and CAC development through its role in macrophage polarization and may serve as a therapeutic target in inflammatory diseases, including IBD

Evidence for Two Modes of Synergistic Induction of Apoptosis by Mapatumumab and Oxaliplatin in Combination with Hyperthermia in Human Colon Cancer Cells

Colorectal cancer is the third leading cause of cancer-related mortality in the world-- the main cause of death from colorectal cancer is hepatic metastases, which can be treated with isolated hepatic perfusion (IHP). Searching for the most clinically relevant approaches for treating colorectal metastatic disease by isolated hepatic perfusion (IHP), we developed the application of oxaliplatin concomitantly with hyperthermia and humanized death receptor 4 (DR4) antibody mapatumumab (Mapa), and investigated the molecular mechanisms of this multimodality treatment in human colon cancer cell lines CX-1 and HCT116 as well as human colon cancer stem cells Tu-12, Tu-21 and Tu-22. We showed here, in this study, that the synergistic effect of the multimodality treatment-induced apoptosis was caspase dependent and activated death signaling via both the extrinsic apoptotic pathway and the intrinsic pathway. Death signaling was activated by c-Jun N-terminal kinase (JNK) signaling which led to Bcl-xL phosphorylation at serine 62, decreasing the anti-apoptotic activity of Bcl-xL, which contributed to the intrinsic pathway. The downregulation of cellular FLICE inhibitory protein long isoform (c-FLIPL) in the extrinsic pathway was accomplished through ubiquitination at lysine residue (K) 195 and protein synthesis inhibition. Overexpression of c-FLIPL mutant (K195R) and Bcl-xL mutant (S62A) completely abrogated the synergistic effect. The successful outcome of this study supports the application of multimodality strategy to patients with colorectal hepatic metastases who fail to respond to standard chemoradiotherapy that predominantly targets the mitochondrial apoptotic pathway. © 2013 Song et al

Knockdown of Midgut Genes by dsRNA-Transgenic Plant-Mediated RNA Interference in the Hemipteran Insect Nilaparvata lugens

BACKGROUND: RNA interference (RNAi) is a powerful technique for functional genomics research in insects. Transgenic plants producing double-stranded RNA (dsRNA) directed against insect genes have been reported for lepidopteran and coleopteran insects, showing potential for field-level control of insect pests, but this has not been reported for other insect orders. METHODOLOGY/PRINCIPAL FINDINGS: The Hemipteran insect brown planthopper (Nilaparvata lugens Stål) is a typical phloem sap feeder specific to rice (Oryza sativa L.). To analyze the potential of exploiting RNAi-mediated effects in this insect, we identified genes (Nlsid-1 and Nlaub) encoding proteins that might be involved in the RNAi pathway in N. lugens. Both genes are expressed ubiquitously in nymphs and adult insects. Three genes (the hexose transporter gene NlHT1, the carboxypeptidase gene Nlcar and the trypsin-like serine protease gene Nltry) that are highly expressed in the N. lugens midgut were isolated and used to develop dsRNA constructs for transforming rice. RNA blot analysis showed that the dsRNAs were transcribed and some of them were processed to siRNAs in the transgenic lines. When nymphs were fed on rice plants expressing dsRNA, levels of transcripts of the targeted genes in the midgut were reduced; however, lethal phenotypic effects after dsRNA feeding were not observed. CONCLUSIONS: Our study shows that genes for the RNAi pathway (Nlsid-1 and Nlaub) are present in N. lugens. When insects were fed on rice plant materials expressing dsRNAs, RNA interference was triggered and the target genes transcript levels were suppressed. The gene knockdown technique described here may prove to be a valuable tool for further investigations in N. lugens. The results demonstrate the potential of dsRNA-mediated RNAi for field-level control of planthoppers, but appropriate target genes must be selected when designing the dsRNA-transgenic plants