Crystal Structures of the structure-selective nuclease Mus81-Eme1 bound to flap DNA substrates

The Mus81-Eme1 complex is a structure-selective endonuclease with a critical role in the resolution of recombination intermediates during DNA repair after interstrand cross-links, replication fork collapse, or double-strand breaks. To explain the molecular basis of 3 ' flap substrate recognition and cleavage mechanism by Mus81-Eme1, we determined crystal structures of human Mus81-Eme1 bound to various flap DNA substrates. Mus81-Eme1 undergoes gross substrate-induced conformational changes that reveal two key features: (i) a hydrophobic wedge of Mus81 that separates pre- and post-nick duplex DNA and (ii) a 5 ' end binding pocket that hosts the 5 ' nicked end of post-nick DNA. These features are crucial for comprehensive protein-DNA interaction, sharp bending of the 3 ' flap DNA substrate, and incision strand placement at the active site. While Mus81-Eme1 unexpectedly shares several common features with members of the 5 ' flap nuclease family, the combined structural, biochemical, and biophysical analyses explain why Mus81-Eme1 preferentially cleaves 3 ' flap DNA substrates with 5 ' nicked ends.X11119Ysciescopu

ATP-dependent DNA binding, unwinding, and resection by the Mre11/Rad50 complex

ATP-dependent DNA end recognition and nucleolytic processing are central functions of the Mre11/Rad50 (MR) complex in DNA double-strand break repair. However, it is still unclear how ATP binding and hydrolysis primes the MR function and regulates repair pathway choice in cells. Here, Methanococcus jannaschii MR-ATP gamma S-DNA structure reveals that the partly deformed DNA runs symmetrically across central groove between two ATP gamma S-bound Rad50 nucleotide-binding domains. Duplex DNA cannot access the Mre11 active site in the ATP-free full-length MR complex. ATP hydrolysis drives rotation of the nucleotide-binding domain and induces the DNA melting so that the substrate DNA can access Mre11. Our findings suggest that the ATP hydrolysis-driven conformational changes in both DNA and the MR complex coordinate the melting and endonuclease activity.1120Ysciescopu

Study of Charmless Hadronic B Meson Decays to Pseudoscalar-Vector Final States

We report results of searches for charmless hadronic B meson decays to pseudoscalar(pi^+-,K^+-,Pi^0 or Ks^0)-vector(Rho, K* or Omega) final states. Using 9.7 million BBbar pairs collected with the CLEO detector, we report first observation of B^- --> Pi^-Rho^0, B^0 --> Pi^+-Rho^-+ and B^- --> Pi^-Omega, which are expected to be dominated by hadronic b --> u transitions. The measured branching fractions are (10.4+3.3-3.4+-2.1)x10^-6, (27.6+8.4-7.4+-4.2)x10^-6 and (11.3+3.3-2.9+-1.4)x10^-6, respectively. Branching fraction upper limits are set for all the other decay modes investigated.Comment: 10 pages postscript, also available through http://w4.lns.cornell.edu/public/CLN

Hadronic Structure in the Decay τ−→π−π0ντ\tau^{-}\to \pi^{-}\pi^{0}\nu_{\tau}

We report on a study of the invariant mass spectrum of the hadronic system in the decay tau- -> pi- pi0 nu_tau. This study was performed with data obtained with the CLEO II detector operating at the CESR e+ e- collider. We present fits to phenomenological models in which resonance parameters associated with the rho(770) and rho(1450) mesons are determined. The pi- pi0 spectral function inferred from the invariant mass spectrum is compared with data on e+ e- -> pi+ pi- as a test of the Conserved Vector Current theorem. We also discuss the implications of our data with regard to estimates of the hadronic contribution to the muon anomalous magnetic moment.Comment: 39 pages postscript, also available through http://w4.lns.cornell.edu/public/CLN

Observation of Radiative Leptonic Decay of the Tau Lepton

Using 4.68 fb^{-1} of e^+e^- annihilation data collected with the CLEO II detector at the Cornell Electron Storage Ring (CESR) we have studied tau radiative decays tau -> mu nu nu gamma and tau -> e nu nu gamma. For a 10 MeV minimum photon energy in the tau rest frame, the branching fraction of radiative tau decay to a muon or electron is measured to be (3.61+-0.16+-0.35)*10^{-3} or (1.75+-0.06+-0.17)*10^{-2}, respectively. The branching fractions are in agreement with the Standard Model theoretical predictions.Comment: 11 pages postscript, also available through http://w4.lns.cornell.edu/public/CLN

A Measurement of the Decay Asymmetry Parameters in \Xi_{c}^{0}\to \X^{-}\pi^{+}

Using the CLEO II detector at the Cornell Electron Storage Ring we have measured the $\Xi_c^{0}$ decay asymmetry parameter in the decay $\Xi_c^{0} \to \Xi^{-} \pi^+$. We find $\alpha_{\Xi_c^{0}} \alpha_{\Xi} = 0.26 \pm 0.18{(stat)}^{+0.05}_{-0.04}{(syst)}$, using the world average value of $\alpha_{\Xi} = -0.456 \pm 0.014$ we obtain $\alpha_{\Xi_c^{0}} = -0.56 \pm 0.39{(stat)}^{+0.10}_{-0.09}{(syst)}$. The physically allowed range of a decay asymmetry parameter is $-1<\alpha<+1$. Our result prefers a negative value: $\alpha_{\Xi_c^{0}}$ is $<0.1$ at the 90% CL. The central value occupies the middle of the theoretically expected range but is not yet precise enough to choose between models.Comment: 10 pages postscript, also available through http://w4.lns.cornell.edu/public/CLN

Limit on Tau Neutrino Mass from τ−→π−π+π−π0ντ\tau^{-}\to \pi^{-}\pi^{+}\pi^{-}\pi^{0}\nu_{\tau}

From a data sample of 29058 $\tau^\pm\to\pi^\pm\pi^+\pi^-\pi^0\nu_\tau$ decays observed in the CLEO detector we derive a 95% confidence upper limit on the tau neutrino mass of 28 MeV.Comment: 17 pages postscript, also available through http://w4.lns.cornell.edu/public/CLN

Search for Lepton-Flavor-Violating Decays of B Mesons

We have searched a sample of 9.6 million BB-bar events for the lepton-flavor-violating decays B --> h e^{+-} mu^{-+}, B^+ --> h^- e^+ e^+, B^+ --> h^- e^+ mu^+, and B^+ --> h^- mu^+ mu^+, where h is pi, K, rho, and K*(892), a total of sixteen modes. We find no evidence for these decays, and place 90% confidence level upper limits on their branching fractions that range from 1.0 to 8.3 X 10^{-6}.Comment: 8 pages postscript, also available through http://w4.lns.cornell.edu/public/CLNS, PRD R