841 research outputs found

    Dual-frequency addressed hybrid-aligned nematic liquid crystal

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    Dual-frequency addressed hybrid-aligned nematic (HAN) liquid crystal cell is demonstrated as a variable optical attenuator at 1.55 mum wavelength. By controlling the low- and high-frequency electric field, the dual-frequency liquid crystal (DFLC) molecules can be reoriented parallel or perpendicular to the substrates so that the maximum obtainable phase modulation is doubled. In comparison to a homogeneous cell, the DFLC HAN cell shows a lower operating voltage and faster response time. Furthermore, the DFLC HAN cell exhibits three stable states that have some applications such as ternary photonic devices

    Disruption of the Gene Encoding Endo-Ī²-1, 4-Xylanase Affects the Growth and Virulence of Sclerotinia sclerotiorum

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    Sclerotinia sclerotiorum (Lib.) de Bary is a devastating fungal pathogen with worldwide distribution. S. sclerotiorum is a necrotrophic fungus that secretes many cell wall-degrading enzymes (CWDEs) that destroy plantā€™s cell-wall components. Functional analyses of the genes that encode CWEDs will help explain the mechanisms of growth and pathogenicity of S. sclerotiorum. Here, we isolated and characterized a gene SsXyl1 that encoded an endo-Ī²-1, 4-xylanase in S. sclerotiorum. The SsXyl1 expression showed a slight increase during the development and germination stages of sclerotia and a dramatic increase during infection. The expression of SsXyl1 was induced by xylan. The SsXyl1 deletion strains produce aberrant sclerotia that could not germinate to form apothecia. The SsXyl1 deletion strains also lost virulence to the hosts. This study demonstrates the important roles of endo-Ī²-1, 4-xylanase in the growth and virulence of S. sclerotiorum

    Tissue-Specific Expression of the Chicken Calpain2 Gene

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    We quantified chicken calpain 2 (CAPN2) expression in two Chinese chicken breeds (mountainous black-bone chicken breed [MB] and a commercial meat type chicken breed [S01]) to discern the tissue and ontogenic expression pattern and its effect on muscle metabolism. Real-time quantitative PCR assay was developed for accurate measurement of the CAPN2 mRNA expression in various tissues from chickens of different ages (0, 2, 4, 6, 8, 10, and 12 weeks). Results showed that the breast muscle and leg muscle tissues had the highest expression of CAPN2 compared to the other tissues from the same individual (P < .05). Overall, the CAPN2 mRNA level exhibited a ā€œriseā€ developmental change in all tissues. The S01 chicken had a higher expression of the CAPN2 mRNA in all tissues than the MB chicken. Our results suggest that chicken CAPN2 expression may be related to chicken breeds and tissues

    Identification and association of the single nucleotide polymorphisms in calpain3 (CAPN3) gene with carcass traits in chickens

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    <p>Abstract</p> <p>Background</p> <p>The aim of this study is to screen single nucleotide polymorphisms (SNP) of chicken <it>Calpain3 </it>(<it>CAPN3</it>) gene and to analyze the potential association between <it>CAPN3 </it>gene polymorphisms and carcass traits in chickens. We screened <it>CAPN3 </it>single nucleotide polymorphisms (SNP) in 307 meat-type quality chicken from 5 commercial pure lines (S01, S02, S03, S05, and D99) and 4 native breeds from Guangdong Province (Huiyang Huxu chicken and Qingyuan Ma chicken) and Sichuan Province (Caoke chicken and Shandi Black-bone chicken), China.</p> <p>Results</p> <p>Two SNPs (11818T>A and 12814T>G) were detected by single strand conformation polymorphism (SSCP) method and were verified by DNA sequencing. Association analysis showed that the 12814T>G genotypes were significantly associated with body weight (BW), carcass weight (CW), breast muscle weight (BMW), and leg muscle weight (LMW). Haplotypes constructed on the two SNPs (H1, TG; H2, TT; H3, AG; and H4, AT) were associated with BW, CW (<it>P </it>< 0.05), eviscerated percentage (EP), semi-eviscerated percentage (SEP), breast muscle percentage (BMP), and leg muscle percentage (LMP) (<it>P </it>< 0.01). Diplotype H1H2 was dominant for BW, CW, and LMP, and H2H2 was dominant for EP, SEP, and BMP.</p> <p>Conclusion</p> <p>We speculated that the <it>CAPN3 </it>gene was a major gene affecting chicken muscle growth and carcass traits or it was linked with the major gene(s). Diplotypes H1H2 and H2H2 might be advantageous for carcass traits.</p

    Retrieving Soil and Vegetation Temperatures From Dual-Angle and Multipixel Satellite Observations

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    Land surface component temperatures (LSCTs), i.e., the temperatures of soil and vegetation, are important parameters in many applications, such as estimating evapotranspiration and monitoring droughts. However, the multiangle algorithm is affected due to different spatial resolution between nadir and oblique views. Therefore, we propose a combined retrieval algorithm that uses dual-angle and multipixel observations together. The sea and land surface temperature radiometer onboard ESA\u27s Sentinel-3 satellite allows for quasi-synchronous dual-angle observations, from which LSCTs can be retrieved using dual-angle and multipixel algorithms. The better performance of the combined algorithm is demonstrated using a sensitivity analysis based on a synthetic dataset. The spatial errors in the oblique view due to different spatial resolution can reach 4.5 K and have a large effect on the multiangle algorithm. The introduction of multipixel information in a window can reduce the effect of such spatial errors, and the retrieval results of LSCTs can be further improved by using multiangle information for a pixel. In the validation, the proposed combined algorithm performed better, with LSCT root mean squared errors of 3.09 K and 1.91 K for soil and vegetation at a grass site, respectively, and corresponding values of 3.71 K and 3.42 K at a sparse forest site, respectively. Considering that the temperature differences between components can reach 20 K, the results confirm that, in addition to a pixel-average LST, the combined retrieval algorithm can provide information on LSCTs. This article demonstrates the potential of utilizing additional information sources for better LSCT results, which makes the presented combined strategy a promising option for deriving large-scale LSCT products
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