Exploiting Contextual Information for Prosodic Event Detection Using Auto-Context

Prosody and prosodic boundaries carry significant information regarding linguistics and paralinguistics and are important aspects of speech. In the field of prosodic event detection, many local acoustic features have been investigated; however, contextual information has not yet been thoroughly exploited. The most difficult aspect of this lies in learning the long-distance contextual dependencies effectively and efficiently. To address this problem, we introduce the use of an algorithm called auto-context. In this algorithm, a classifier is first trained based on a set of local acoustic features, after which the generated probabilities are used along with the local features as contextual information to train new classifiers. By iteratively using updated probabilities as the contextual information, the algorithm can accurately model contextual dependencies and improve classification ability. The advantages of this method include its flexible structure and the ability of capturing contextual relationships. When using the auto-context algorithm based on support vector machine, we can improve the detection accuracy by about 3% and F-score by more than 7% on both two-way and four-way pitch accent detections in combination with the acoustic context. For boundary detection, the accuracy improvement is about 1% and the F-score improvement reaches 12%. The new algorithm outperforms conditional random fields, especially on boundary detection in terms of F-score. It also outperforms an n-gram language model on the task of pitch accent detection

Design of a novel closed-loop SOI MEMS resonant electrostatic field sensor

AbstractA novel closed-loop SOI MEMS resonant electrostatic field sensor (EFS) has been designed in this paper. The sensor architecture has three major blocks: a vibration shutter, sensing electrodes, and a closed-loop driving electrode that feeds back to the shutter. With a phase and an amplitude closed loop feedback control, the sensor can be adjusted automatically to operate at its resonant frequency. Prototyped by the SOI fabrication process, the device has a lower driving voltage and a higher quality factor (Q) over existing electrostatic comb driven EFS. An improved uncertainty of 2.1% is achieved for the SOI device operating in ambient air in a measured range of 0–50 kV/m

Research Progress on the Fish Complement C3 Gene

Complement (C) is a serum protein with immunity-related functions and enzymatic activities. It can induce inflammatory responses and antibody formation following activation by substances such as pathogens. Moreover, it is a crucial molecule in the congenital immune defense system. Complement manifests earlier than immunoglobulin in the evolutionary process of fish's immune system. Therefore, the complement system of fish is vital for congenital and acquired immunities. A complement system is a polymolecule system comprising more than 30 soluble proteins, membrane-bound proteins, and complement receptors. It can be activated through three pathways for immunoregulation, the clearance of the immune complex, etc. The liver primarily produces the fish complement C3, which is a significant component of the complement system of fishes. C3 is the hub of three activation pathways. Research works concerning the structure, generation process, expression, and functions of C3 in fishes are reviewed in this study

Humidity-Induced Charge Leakage and Field Attenuation in Electric Field Microsensors

The steady-state zero output of static electric field measuring systems often fluctuates, which is caused mainly by the finite leakage resistance of the water film on the surface of the electric field microsensor package. The water adsorption has been calculated using the Boltzmann distribution equation at various relative humidities for borosilicate glass and polytetrafluoroethylene surfaces. At various humidities, water film thickness has been calculated, and the induced charge leakage and field attenuation have been theoretically investigated. Experiments have been performed with microsensors to verify the theoretical predictions and the results are in good agreement

31st Annual Meeting and Associated Programs of the Society for Immunotherapy of Cancer (SITC 2016) : part two

Background The immunological escape of tumors represents one of the main ob- stacles to the treatment of malignancies. The blockade of PD-1 or CTLA-4 receptors represented a milestone in the history of immunotherapy. However, immune checkpoint inhibitors seem to be effective in specific cohorts of patients. It has been proposed that their efficacy relies on the presence of an immunological response. Thus, we hypothesized that disruption of the PD-L1/PD-1 axis would synergize with our oncolytic vaccine platform PeptiCRAd. Methods We used murine B16OVA in vivo tumor models and flow cytometry analysis to investigate the immunological background. Results First, we found that high-burden B16OVA tumors were refractory to combination immunotherapy. However, with a more aggressive schedule, tumors with a lower burden were more susceptible to the combination of PeptiCRAd and PD-L1 blockade. The therapy signifi- cantly increased the median survival of mice (Fig. 7). Interestingly, the reduced growth of contralaterally injected B16F10 cells sug- gested the presence of a long lasting immunological memory also against non-targeted antigens. Concerning the functional state of tumor infiltrating lymphocytes (TILs), we found that all the immune therapies would enhance the percentage of activated (PD-1pos TIM- 3neg) T lymphocytes and reduce the amount of exhausted (PD-1pos TIM-3pos) cells compared to placebo. As expected, we found that PeptiCRAd monotherapy could increase the number of antigen spe- cific CD8+ T cells compared to other treatments. However, only the combination with PD-L1 blockade could significantly increase the ra- tio between activated and exhausted pentamer positive cells (p= 0.0058), suggesting that by disrupting the PD-1/PD-L1 axis we could decrease the amount of dysfunctional antigen specific T cells. We ob- served that the anatomical location deeply influenced the state of CD4+ and CD8+ T lymphocytes. In fact, TIM-3 expression was in- creased by 2 fold on TILs compared to splenic and lymphoid T cells. In the CD8+ compartment, the expression of PD-1 on the surface seemed to be restricted to the tumor micro-environment, while CD4 + T cells had a high expression of PD-1 also in lymphoid organs. Interestingly, we found that the levels of PD-1 were significantly higher on CD8+ T cells than on CD4+ T cells into the tumor micro- environment (p < 0.0001). Conclusions In conclusion, we demonstrated that the efficacy of immune check- point inhibitors might be strongly enhanced by their combination with cancer vaccines. PeptiCRAd was able to increase the number of antigen-specific T cells and PD-L1 blockade prevented their exhaus- tion, resulting in long-lasting immunological memory and increased median survival

Fabrication of a Miniature Multi-Parameter Sensor Chip for Water Quality Assessment

Water contamination is a main inducement of human diseases. It is an important step to monitor the water quality in the water distribution system. Due to the features of large size, high cost, and complicated structure of traditional water determination sensors and devices, it is difficult to realize real-time water monitoring on a large scale. In this paper, we present a multi-parameter sensor chip, which is miniature, low-cost, and robust, to detect the pH, conductivity, and temperature of water simultaneously. The sensor chip was fabricated using micro-electro-mechanical system (MEMS) techniques. Iridium oxide film was electrodeposited as the pH-sensing material. The atomic ratio of Ir(III) to Ir(IV) is about 1.38 according to the X-ray photoelectron spectroscopy (XPS) analysis. The pH sensing electrode showed super-Nernstian response (−67.60 mV/pH) and good linearity (R2 = 0.9997), in the range of pH 2.22 to pH 11.81. KCl-agar and epoxy were used as the electrolyte layer and liquid junction for the solid-state reference electrode, respectively, and its potential stability in deionized water was 56 h. The conductivity cell exhibited a linear determination range from 21.43 μ S / cm to 1.99 mS / cm , and the electrode constant was 1.566 cm−1. Sensitivity of the temperature sensor was 5.46 Ω / ° C . The results indicate that the developed sensor chip has potential application in water quality measurements

Unveiling oil-additive/surface hierarchy at real ring-liner contact

Zinc-dialkyldithiophosphate (ZDDP) additive in oil has been extensively reported to form a protective tribofilm between the moving components of engine for preventing wear. In this study, surface and interface characterizations were conducted on real piston ring/cylinder liner contacts, the interacting mechanism between oil-additive and solid surfaces under realistic mechanical and thermal conditions was predicted according to the identification of the chemical composition and structure of the tribofilm. The results demonstrate the interesting laminated hierarchy of tribofilm. This tribofilm is composed mainly of dense carbon precipitation at the top region and a thinner polyphosphate intermediate layer in contact with deformed iron oxide substrate on the cylinder liner. The depletion of polyphosphate compounds and competitive growth of solid carbon in term of lubricant\u27s degradation was speculated. These significant findings help manufacturer to understand possible tribochemical processes occurring in the real tribosystem from the practical view, and in particular inspire researchers to develop the new strategy to improve capability and efficiency of oil-additive at sliding contacts

A Mediated BOD Biosensor Based on Immobilized B. Subtilis on Three-Dimensional Porous Graphene-Polypyrrole Composite

We have developed a novel mediated biochemical oxygen demand (BOD) biosensor based on immobilized Bacillus subtilis (B. subtilis) on three-dimensional (3D) porous graphene-polypyrrole (rGO-PPy) composite. The 3D porous rGO-PPy composite was prepared using hydrothermal method following with electropolymerization. Then the 3D porous rGO-PPy composite was used as a support for immobilizing negatively charged B. subtilis denoted as rGO-PPy-B through coordination and electrostatic interaction. Further, the prepared rGO-PPy-B was used as a microbial biofilm for establishing a mediated BOD biosensor with ferricyanide as an electronic acceptor. The indirect determination of BOD was performed by electrochemical measuring ferrocyanide generated from a reduced ferricyanide mediator using interdigited ultramicroelectrode array (IUDA) as the working electrode. The experimental results suggested a good linear relationship between the amperometric responses and BOD standard concentrations from 4 to 60 mg/L, with a limit detection of 1.8 mg/L (S/N ≥ 3). The electrochemical measurement of real water samples showed a good agreement with the conventional BOD5 method, and the good anti-interference as well as the long-term stability were well demonstrated, indicating that the proposed mediated BOD biosensor in this study holds a potential practical application of real water monitoring