212 research outputs found
Morphological changes in human serum albumin in presence of cationic amphiphilic drugs
- Publication venue
- 'Royal Society of Chemistry (RSC)'
- Publication date
- 05/01/2018
- Field of study
Human serum albumin (HSA) is one of the most important carrier proteins present in the blood and can constitute more than half of serum proteins. It transports various biomolecules including hormones, fatty acids, ions, drugs and functions to regulate oncotic pressure in the plasma. Cationic amphiphillic drugs like amitriptyline hydrochloride, imipramine hydrochloride and promethazine hydrochloride bind to HSA and influences function by altering its conformation, as confirmed by Small-angle neutron scattering (SANS) data coupled to dynamic light scattering measurements (DLS). Protein unfolding was observed by SANS results through an increase in the value of the radius of gyration Rg. At higher drug concentrations, there was no change in the dimensions of the protein. However, the drugs formed free aggregates at higher concentrations without any growth in the drug micelles, which was confirmed by the appearance of second peak in DLS measurements. Molecular docking revealed that the morphology of hydrophobic moiety of the cationic amphiphilic drugs decides their binding fate with HSA, while trajectories from molecular dynamics simulations highlight structural disorder in the drug-HSA complex
Quantitative Whole Body Biodistribution of Fluorescent-Labeled Agents by Non-Invasive Tomographic Imaging
- Author
- A Banzato
- A Priyadarshani
- A Robe
- A Wunder
- C Buono
- C. Andrew Boswell
- Chelsea Casavant
- DS Boss
- H Korideck
- J Grimm
- J Peterson
- JD Peterson
- Jeffrey D. Peterson
- KM Kozloff
- Kristine O. Vasquez
- L Stangenberg
- M Nahrendorf
- M Tabrizi
- MA Yaseen
- ME Ackerman
- P Mohajerani
- RF Hoyt
- S Kossodo
- U Mahmood
- V Kenanova
- V Ntziachristos
- V Ntziachristos
- X Montet
- Y Seo
- Y Takakura
- Y Zilberman
- Z Yang
- Z Zhang
- Publication venue
- Public Library of Science
- Publication date
- 01/01/2011
- Field of study
When small molecules or proteins are injected into live animals, their physical and chemical properties will significantly affect pharmacokinetics, tissue penetration, and the ultimate routes of metabolism and clearance. Fluorescence molecular tomography (FMT) offers the ability to non-invasively image and quantify temporal changes in fluorescence throughout the major organ systems of living animals, in a manner analogous to traditional approaches with radiolabeled agents. This approach is best used with biotherapeutics (therapeutic antibodies, or other large proteins) or large-scaffold drug-delivery vectors, that are minimally affected by low-level fluorophore conjugation. Application to small molecule drugs should take into account the significant impact of fluorophore labeling on size and physicochemical properties, however, the presents studies show that this technique is readily applied to small molecule agents developed for far-red (FR) or near infrared (NIR) imaging. Quantification by non-invasive FMT correlated well with both fluorescence from tissue homogenates as well as with planar (2D) fluorescence reflectance imaging of excised intact organs (r2 = 0.996 and 0.969, respectively). Dynamic FMT imaging (multiple times from 0 to 24 h) performed in live mice after the injection of four different FR/NIR-labeled agents, including immunoglobulin, 20–50 nm nanoparticles, a large vascular imaging agent, and a small molecule integrin antagonist, showed clear differences in the percentage of injected dose per gram of tissue (%ID/g) in liver, kidney, and bladder signal. Nanoparticles and IgG1 favored liver over kidney signal, the small molecule integrin-binding agent favored rapid kidney and bladder clearance, and the vascular agent, showed both liver and kidney clearance. Further assessment of the volume of distribution of these agents by fluorescent volume added information regarding their biodistribution and highlighted the relatively poor extravasation into tissue by IgG1. These studies demonstrate the ability of quantitative FMT imaging of FR/NIR agents to non-invasively visualize and quantify the biodistribution of different agents over time
Burden-driven feedback control of gene expression
- Author
- A Casini
- A Gupta
- A Gyorgy
- AAK Nielsen
- Ali R Awan
- Alice Boo
- AP Arkin
- AY Weiße
- BK Lohman
- BS Der
- C Tan
- CG Kurland
- Charlie Gilbert
- CJ Myers
- DE Cameron
- E Guisbert
- F Ceroni
- F Moser
- Francesca Ceroni
- G Nonaka
- GA Brar
- Guy-Bart Stan
- H El-Samad
- H Kurata
- I Farasat
- I Shachrai
- IM Keseler
- J Carrera
- J Gertz
- JR Houser
- K Nakahigashi
- L Jiang
- M Dragosits
- M Lynch
- M Pasini
- MI Love
- O Borkowski
- Olivier Borkowski
- R Edgar
- S Cardinale
- S He
- SC Sleight
- Simone Furini
- TE Gorochowski
- TE Gorochowski
- TH Segall-Shapiro
- Thomas E Gorochowski
- Tom Ellis
- X Zhang
- Y Qian
- Yaseen N Ladak
- Z Wang
- Publication venue
- 'Cold Spring Harbor Laboratory'
- Publication date
- 01/08/2017
- Field of study
Cells use feedback regulation to ensure robust growth despite fluctuating demands for resources and differing environmental conditions. However, the expression of foreign proteins from engineered constructs is an unnatural burden that cells are not adapted for. Here we combined RNA-seq with an in vivo assay to identify the major transcriptional changes that occur in Escherichia coli when inducible synthetic constructs are expressed. We observed that native promoters related to the heat-shock response activated expression rapidly in response to synthetic expression, regardless of the construct. Using these promoters, we built a dCas9-based feedback-regulation system that automatically adjusts the expression of a synthetic construct in response to burden. Cells equipped with this general-use controller maintained their capacity for native gene expression to ensure robust growth and thus outperformed unregulated cells in terms of protein yield in batch production. This engineered feedback is to our knowledge the first example of a universal, burden-based biomolecular control system and is modular, tunable and portable
Effects of dietary postbiotic and inulin on growth performance, IGF1 and GHR mRNA expression, faecal microbiota and volatile fatty acids in broilers
- Author
- A Ashayerizadeh
- A Nabizadeh
- A Rebolé
- A Santos
- AM Shareef
- Anjas Asmara Samsudin
- AS Elrayeh
- BR Beckman
- BR Beckman
- BR Beckman
- C Beccavin
- D Choe
- D Józefiak
- G Gebbink
- GA Kleter
- H Foo
- H Foo
- H Hajati
- Henny Akit
- Hooi Ling Foo
- HU Rehman
- J Kadlec
- JI Jones
- JT Silverstein
- Karwan Yaseen Kareem
- KY Kareem
- KY Kareem
- L Montagne
- L Nankervis
- LG Aristides
- LV Langen
- MJ Shamblott
- MR Rosyidah
- N Shazali
- N Thanh
- N Zhou
- P Biggs
- P Marteau
- PW Wielen Van der
- S Jung
- S Langlands
- S Moriyama
- SA Bolu
- SM Abdel-Raheem
- T Thu
- TC Loh
- TC Loh
- Teck Chwen Loh
- X Li
- X Liu
- Y Li
- Y Yang
- Z Uni
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- Field of study
In vitro nuclear interactome of the HIV-1 Tat protein
- Author
- A Clery
- A Gatignol
- A Kuzmichev
- A Kuzmichev
- A Marchler-Bauer
- A Mazumdar
- A Munnia
- A Pumfery
- A You
- AB Makar
- AJ Ruthenburg
- AM Hidalgo-Estevez
- AM Pyle
- AS Neish
- AY Nikolaev
- B Kalverda
- BB Singh
- BJ Breitkreutz
- BJ Wilson
- BK Das
- BM Lunde
- BP Ashburner
- C Alfarano
- C Delphin
- C Franz
- C Hetzer
- C Morris-Desbois
- C Musahl
- C Schwerk
- C Treand
- C Underhill
- CA Hassig
- CA Hassig
- CA Johnson
- CA Johnson
- CA Parada
- CD Laherty
- CG Lee
- CH Herrmann
- CK Dreger
- CL Jiang
- CL Will
- CM Grozinger
- D Dorner
- D Gorlich
- D Li
- D Maiorano
- D Reinberg
- D Schulte
- D Yasui
- DA Bochar
- DC Bharucha
- DD Fischer
- DG Quintana
- DR Schmidt
- E Agbottah
- E Cavellan
- E Craig
- E Nagoshi
- E Nicolas
- E Nicolas
- EK Sullivan
- EP Kransdorf
- ER Griffis
- F Kashanchi
- F Macian
- F Peruzzi
- FR Bischoff
- G Dellaire
- G He
- G Jiang
- G Lattanzi
- GL Mayeur
- H Cho
- H Fujita
- H Kato
- H Kitagawa
- H Sakai
- H Takeuchi
- H Xiao
- H Zhang
- H Zhang
- HH Ng
- HL Kiefer
- HT Adler
- I Letunic
- I Olave
- J Becker
- J Boeke
- J Brady
- J Harborth
- J Joseph
- J Kamine
- J Koipally
- J Koipally
- J Koipally
- J Marango
- J Moroianu
- J Park
- J Taplick
- J van der Vlag
- J Zhou
- JA Schmiesing
- JA Schmiesing
- JF Rual
- JH Lee
- JH Liu
- JJ Coull
- JK Tong
- JM Bridger
- JR Dobosy
- JW Critchfield
- K Furukawa
- K Imai
- K Scheffzek
- K Yoder
- K Zhao
- K Zhou
- KC Moraes
- KI Tatematsu
- KL Block
- KM Lounsbury
- KS McKeegan
- L Corsini
- L Deng
- L Finch
- L Mohrmann
- L Tickenbrock
- L Yang
- LA Boyer
- LF Garcia-Martinez
- Lili Gu
- LR Racki
- M Ashburner
- M Barboric
- M Benkirane
- M Bienz
- M Brackertz
- M Brackertz
- M Brand
- M Bukrinsky
- M Chibi
- M Fornerod
- M Fuchs
- M Fujita
- M Kneissl
- M Kohler
- M Meurer
- M Ohnishi
- M Ott
- M Saito
- M Sorin
- M Stros
- M Tyagi
- M Yanagida
- MA Bhat
- MA Hakimi
- MA Hakimi
- MA Hakimi
- MJ Bottomley
- ML Phelan
- MR Rountree
- MV Natsiuk
- MY Chou
- N Bonifaci
- N Epie
- N Fujita
- N Methot
- N Wagner
- N Yabuta
- Niaobh O'Donoghue
- NJ Watkins
- Noreen Sheehy
- NR Yaseen
- NR Yaseen
- O Rohr
- O Rozenblatt-Rosen
- P Cramer
- P Gacesa
- PA Tucker
- PG Young
- PM Dehe
- PM Dehe
- Q Ye
- R Berro
- R Gamsjaeger
- R Mahajan
- R Murr
- R Truant
- R Van Duyne
- R Van Duyne
- RA Sclafani
- RA Silverstein
- RC Hillig
- RD Adzerikho
- RD Finn
- RE Kiernan
- RH Kehlenbach
- RM Ewing
- S Bannwarth
- S Debernardi
- S Mujtaba
- S Mujtaba
- S Nakielny
- S Nekhai
- S Nekhai
- S Pagans
- S Peri
- S Shaklai
- S Sif
- S Takezawa
- S Vashee
- SA Ansari
- SA Denslow
- SA Williams
- SC Tsai
- SK Dhar
- SL Forsburg
- SM Nicol
- Stephen Pennington
- T Ammosova
- T Hirano
- T Mahmoudi
- T Otsuki
- T Sasaki
- T Tasara
- T Yoshida
- TC Fleischer
- TE Harris
- TP Cujec
- TP Cujec
- TW Reichman
- U Kutay
- U Stelzl
- V Bres
- VB Cismasiu
- VF Zhupan
- Virginie W Gautier
- VW Gautier
- W Antonin
- W Fischle
- W Fischle
- W Fu
- W Wang
- WE Muller
- WE Muller
- William W Hall
- WM Yang
- X Yang
- Y Ariumi
- Y Bennasser
- Y Doyon
- Y Ishimi
- Y Ishimi
- Y Shi
- Y Xue
- Y Zhang
- Y Zhang
- Y Zhang
- Y Zhang
- Y Zhang
- Y Zhang
- Y Zhu
- YJ Hsieh
- YL Yao
- YL Yao
- YP Li
- YP Li
- Z Nie
- Z You
- Z You
- ZB Xia
- Publication venue
- BioMed Central
- Publication date
- 01/01/2009
- Field of study
<p>Abstract</p> <p>Background</p> <p>One facet of the complexity underlying the biology of HIV-1 resides not only in its limited number of viral proteins, but in the extensive repertoire of cellular proteins they interact with and their higher-order assembly. HIV-1 encodes the regulatory protein Tat (86–101aa), which is essential for HIV-1 replication and primarily orchestrates HIV-1 provirus transcriptional regulation. Previous studies have demonstrated that Tat function is highly dependent on specific interactions with a range of cellular proteins. However they can only partially account for the intricate molecular mechanisms underlying the dynamics of proviral gene expression. To obtain a comprehensive nuclear interaction map of Tat in T-cells, we have designed a proteomic strategy based on affinity chromatography coupled with mass spectrometry.</p> <p>Results</p> <p>Our approach resulted in the identification of a total of 183 candidates as Tat nuclear partners, 90% of which have not been previously characterised. Subsequently we applied <it>in silico </it>analysis, to validate and characterise our dataset which revealed that the Tat nuclear interactome exhibits unique signature(s). First, motif composition analysis highlighted that our dataset is enriched for domains mediating protein, RNA and DNA interactions, and helicase and ATPase activities. Secondly, functional classification and network reconstruction clearly depicted Tat as a polyvalent protein adaptor and positioned Tat at the nexus of a densely interconnected interaction network involved in a range of biological processes which included gene expression regulation, RNA biogenesis, chromatin structure, chromosome organisation, DNA replication and nuclear architecture.</p> <p>Conclusion</p> <p>We have completed the <it>in vitro </it>Tat nuclear interactome and have highlighted its modular network properties and particularly those involved in the coordination of gene expression by Tat. Ultimately, the highly specialised set of molecular interactions identified will provide a framework to further advance our understanding of the mechanisms of HIV-1 proviral gene silencing and activation.</p
7th Drug hypersensitivity meeting: part two
- Author
- Abuzgaia A.M.
- Acar E.A.
- Aguiar R.
- Aihara M.
- Aktas A.
- Al-Attar Z.
- Albert F.
- Alfirevic A.
- Alhaidari M.
- Alhilali K.
- Alonso A.B.
- Alvarez A.
- Alzahrani A.
- Amaral L.
- Andreu I.
- Antipkin Y.
- Aouam K.
- Aoyama Y.
- Aranda C.
- Ardeniz F.Ö.
- Ardern-Jones M.R.
- Ariza A.
- Arreola A.M.
- Arribas F.
- Aumiler M.R.
- Ausperger K.M.
- Azoury M.E.
- Azukizawa H.
- Balakirski G.
- Barbaud A.
- Baron J.M.
- Barona C.T.
- Barreira P.
- Barrionuevo E.
- Barzylovych V.
- Bauvin O.
- Baysal T.
- Bechara R.
- Beeghly-Fadiel A.
- Bellón T.
- Belo J.
- Benito-Garcia F.
- Bensaïd B.
- Bernad A.
- Berry N.
- Betts C.
- Bibián B.L.
- Bindslev-Jensen C.
- Blanca M.
- Blanca-López N.
- Blanco A.J.
- Blanco A.J.
- Blanco M.A.J.
- Bogas G.
- Bom A.T.
- Boni E.
- Borobia A.M.
- Boteanu C.
- Boughattas N.A.
- Bozbey H.U.
- Breynaert C.
- Bridts C.H.
- Bulut G.
- Burgos Pimentel A.M.
- Buyuozturk S.
- Cabañas R.
- Cabezas P.G.
- Cadinha S.
- Campo P.
- Canto G.
- Canto M.G.
- Cardoso B.K.
- Carolino F.
- Carrapatoso I.
- Carreiro-Martins P.
- Castañer J.L.
- Castells M.
- Cegec I.
- Cernadas J.R.
- Chaabane A.
- Chadly Z.
- Chambel M.
- Chang Y-C
- Chang Y-S
- Chen Y-T
- Cheng C.
- Cherit J.D.
- Chiriac A.
- Cho S-H
- Choi J.C.
- Chopra A.
- Chorda C.P.
- Chordá C.P.
- Chow L.
- Chung W-H
- Claude N.
- Colakoglu B.
- Collado D.
- Cornejo-García J.A.
- Corona J.A.B.
- Coronel V.P.
- Correia M.
- Correia S.
- Coskun R.
- Cosme J.
- Cruz C.R.
- Da Silva Franco J.F.
- Da Silva J.P.
- Dalgiç C.T.
- De Abajo F.J.
- De Castro E.D.
- De Francisco A.M.M.
- De La Cruz Hoyos B.
- De La Losa F.P.
- De Rojas D.H.F.
- De Vicente Jiménez T.M.
- Del Carmen Plaza-Serón M.
- Del Pozo M.
- Delahaye C.
- Demir S.
- Deniz G.
- Dias-Castro E.
- Dionicio J.
- Domínguez J.
- Dos Reis R.P.
- Doña I.
- Dörnbach H.
- D’Amelio C.
- Ebo D.G.
- Elera J.D.
- Elzagallaai A.A.
- Ensina L.F.
- Ermertcan A.T.
- Fadhel N.B.
- Faria E.
- Farrel J.
- Farrell J.
- Faulkner L.
- Fernandes R-A.R.
- Fernandez T.
- Fernandez T.
- Fernandez T.D.
- Fernández R.
- Fernández T.D.
- Ferre J.F.
- Ferrer M.
- Fettke H.
- Fiandor A.
- Fiandor A.M.
- Figueroa N.V.D.
- Fili L.
- Flabbee J.
- Flores C.
- Flores J.L.C.
- Flores S.M.
- Fredj N.B.
- French N.S.
- Fujiyama T.
- Galindo M.L.
- Galvao V.R.
- Gancedo S.Q.
- García I.C.
- García-Agúndez J.A.
- García-Martín E.
- Garrido J.M.
- Garófalo C.D.
- Gaspar Â.
- Gastaminza G.
- Gelincik A.
- Gibson A.
- Gilissen L.
- Goikoetxea M.J.
- Gomis V.S.
- Gonzalez-Mendiola R.
- González-Morena J.M.
- Goossens A.
- Goudouris E.
- Guerrero M.A.
- Gulin S.J.
- Guzmán A.E.
- Guéant J-L
- Guéant-Rodríguez R.M.
- Gökmen E.N.M.
- Gülbahar O.
- Günsen F.D.
- Hanafusa T.
- Harrison C.J.
- Hashizume H.
- Haw W.Y.
- Ho R.
- Hofmeier K.S.
- Hui C-Y.R.
- Hung S-L
- Huygens S.
- Illing P.
- Intusoma U.
- Inácio F.
- Irigoyen R.F.
- Ito T.
- Jantararoungtong T.
- Jenkins R.
- Jenkins R.E.
- Joseph D.
- Jurado-Escobar R.
- Jurakic-Toncic R.
- Kanagawa O.
- Kang H-R
- Katayama I.
- Kato K.
- Kevin Park B.
- Kim S-H
- Kim S-S
- Kinoshita S.
- Klaewsongkram J.
- Ko T-M
- Kokuludag A.
- Konvinse K.
- Koomdee N.
- Korosec P.
- Kraljickovic I.
- Kwan P.
- Lacerda A.
- Lafuente A.
- Laguna J.J.
- Lai J.
- Lapresa M.J.G.
- Lapshyn V.
- Lasarte G.G.
- Leary S.
- Lee H.Y.
- Lee J.J.
- Leiria-Pinto P.
- Leverkus M.
- Leão L.C.
- Lin C-Y
- Ljubojevic S.
- Lluncor M.
- Lopes A.
- Lopez J.T.
- Lourenço T.
- Luque A.G.
- Madamba R.C.Y.
- Maggi E.
- Maillere B.
- Malheiro D.
- Mallal S.
- Marques J.
- Marti L.M.T.
- Martin-Serrano A.
- Martinez J.J.L.
- Martins A.M.
- Martín-Serrano Á.
- Maryanski J.
- Matucci A.
- Matute O.V.
- Mauro M.
- Mayorga C.
- Mcguire C.
- McKinnon E.
- Mendoza Parra A.M.
- Meng X.
- Merk H.F.
- Mesa-Antunez P.
- Mifsud N.
- Min K-U
- Miranda P.A.
- Molina A.
- Molina N.
- Montañez M.I.
- Montañez M.I.
- Morais-Almeida M.
- Moreira A.
- Moreno M.R.C.
- Mortz C.G.
- Mota I.
- Mutez V.
- Muñoz M.G.
- Möbs C.
- Nahal D.J.
- Naisbitt D.J.
- Najera F.
- Nakonechna A.
- Nencini F.
- Neves A.C.
- Nhim C.
- Nicolas J-F
- Nunes I.C.
- Núñez J.
- Oda K.
- Ogese M.
- Ogese M.O.
- Olazabal I.
- Olgac M.
- Ortega J.D.
- O’Neill P.M.
- Paes M-J
- Pairayayutakul K.
- Palacios M.D.
- Pallardy M.
- Palomares F.
- Parejo P.A.
- Park H-W
- Parronchi P.
- Pavlos R.
- Peckham D.
- Pereira-Barbosa M.
- Perez-Inestrosa E.
- Perkins J.
- Perkins J.R.
- Pfützner W.
- Phillips E.
- Piedade S.
- Pineda F.
- Pinto N.
- Pires A.P.
- Pirmohamed M.
- Pita J.
- Pola B.
- Polak M.E.
- Poves F.A.
- Pratesi S.
- Puangpetch A.
- Puga M.F.
- Purcell A.
- Pérez-Alzate D.
- Pérez-Inestrosa E.
- Pérez-Sala D.
- Queiroz L.
- Quirce S.
- Ramirez E.
- Rauber M.
- Redwood A.J.
- Reig V.C.
- Rerknimitr P.
- Ribeiro C.
- Ribeiro M.
- Rieder M.J.
- Rodolfo A.
- Rodriguez A.E.
- Roman A.M.F.
- Romano A.
- Romdhane H.B.
- Romero A.M.
- Rozières A.
- Ruiz A.
- Ruiz M.D.
- Rupnik H.
- Russello M.
- Rutkowski K.
- Sabato V.
- Salas M.
- Salazar M.L.
- Salgueiro R.L.
- Sanchez J.F.
- Sanchez M.I.
- Santiago T.L.
- Santon S.
- Satapornpong P.
- Satoh M.
- Sawai H.
- Schrijvers R.
- Scornet N.
- Sen F.
- Shiohara T.
- Silar M.
- Silva D.
- Simic I.
- Sin A.Z.
- Skov P.S.
- Solé D.
- Sotozono C.
- Sousa N.
- Spínola-Santos A.
- Strautins K.
- Sukasem C.
- Sullivan A.
- Sánchez-Gómez F.J.
- Tailor A.
- Tanegashima T.
- Tannert L.K.
- Tardieu M.
- Tassaneeyakul W.
- Temiz C.
- Tempark T.
- Theeramoke V.
- Thinnes A.
- Thomson P.
- Tokunaga K.
- Tokura Y.
- Tomaz E.
- Tong H.
- Torres M.J.
- Torres M.J.
- Torres M.J.
- Torres M.J.
- Torres M.J.
- Trelles O.
- Trubiano J.
- Turcic P.
- Turk V.E.
- Ue K.L.
- Ueta M.
- Umanets T.
- Umayahara T.
- Unal D.
- Upton A.
- Usui T.
- Uyttebroek A.P.
- Valle D.K.
- Van Hoeyveld E.
- Vega O.
- Vida Y.
- Villani A.P.
- Vilà-Nadal G.
- Viseu R.
- Visse J.
- Vocanson M.
- Voronova M.
- Vultaggio A.
- Wada H.
- Watanabe T.
- Waton J.
- Weaver R.
- Whitaker P.
- White K.
- Williams K.
- Wood S.
- Woolnough E.
- Wu J-Y
- Xue Y.
- Yamaguchi Y.
- Yang C-H
- Yang M-S
- Yaseen F.
- Zidarn M.
- Çatin-Aktas E.
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- 01/01/2016
- Field of study
No abstract availabl
Global economic burden of unmet surgical need for appendicitis
- Author
- Aahlin E. K.
- Aamer A.
- Aaniana K.
- Ababneh A.
- Abaliksta T.
- Abantanga F.
- Abbas M.
- Abbasy J.
- Abbo O.
- Abbouch M.
- Abd-Elmawla M.
- Abd-Elrasoul Y.
- Abdallah E.
- Abdel-Aty A.
- Abdel-Hameed N.
- Abdel-Kader S. M.
- Abdel-Wahab N. Y. E.
- Abdelaal A.
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- Publication venue
- Oxford University Press
- Publication date
- 01/01/2022
- Field of study
Background: There is a substantial gap in provision of adequate surgical care in many low-and middle-income countries. This study aimed to identify the economic burden of unmet surgical need for the common condition of appendicitis. Methods: Data on the incidence of appendicitis from 170 countries and two different approaches were used to estimate numbers of patients who do not receive surgery: as a fixed proportion of the total unmet surgical need per country (approach 1); and based on country income status (approach 2). Indirect costs with current levels of access and local quality, and those if quality were at the standards of high-income countries, were estimated. A human capital approach was applied, focusing on the economic burden resulting from premature death and absenteeism. Results: Excess mortality was 4185 per 100 000 cases of appendicitis using approach 1 and 3448 per 100 000 using approach 2. The economic burden of continuing current levels of access and local quality was US 92492millionusingapproach1and73 141 million using approach 2. The economic burden of not providing surgical care to the standards of high-income countries was 95004millionusingapproach1and75 666 million using approach 2. The largest share of these costs resulted from premature death (97.7 per cent) and lack of access (97.0 per cent) in contrast to lack of quality. Conclusion: For a comparatively non-complex emergency condition such as appendicitis, increasing access to care should be prioritized. Although improving quality of care should not be neglected, increasing provision of care at current standards could reduce societal costs substantially
Hyperoxemia and excess oxygen use in early acute respiratory distress syndrome : Insights from the LUNG SAFE study
- Author
- Abad Ricardo Diaz
- Abe Toshikazu
- Abroug Fekri
- Adams John C.
- Adamsson Richard O.
- Adolfsson Anne
- Aguilar Ronald Zumaran
- Aguirre Janet S.
- Aguirre-Bermeo Hernan
- Aisa Tharwat M.
- Aisiku Imo
- Ajetunmobi Olaitan O.
- Aker Mustafa
- Akyol Onat O.
- Al Dorzi Hasan M.
- Al Jabbary Ahmed S.
- Albaiceta Guillermo M.
- Albarrak Mohamad M.
- Albiero Daniela
- Alda Rosa S.
- Alejandro Risso V.
- Alexander Peter
- Algaba Ángela
- Aliaga Felipe A.
- Alisasis Alain U.
- Allan Russell M.
- Allue Raquel Montoiro
- Almekhlafi Ghaleb
- Alonso Javier G.
- Altaf Wahid
- Alvarez António M.
- Alvarez Javier
- Amin Pravin
- Amir Fahd
- Amor Abdellatif Benslama Mourad
- An Youzhong
- Andersen Finn H.
- Andoh Kohkichi
- Andruszkiewicz Pawel
- Aneman Anders
- Antonelli Massimo M.
- Antônio Ana Carolina P.
- Aoki Nobumasa
- Appelberg Henrik
- Arabi Yaasen
- Arabi Yaseen M.
- Araujo Ana M.
- Arizmendi Alfons
- Arnal Jean-Michel
- Artiga Maria Jose D.
- Artigas Antonio
- Ascoli Aline
- Au Siu-Ming
- Avila Luis E.
- Azad Raman
- Azevedo Luciano Cesar
- Baboi Loredana
- Badie Julio C.
- Bailey Irene M.
- Bailey Mathew B.
- Bajwa Ed
- Balkhi Hicham
- Baltus Thomas
- Banner-Goodspeed Valerie M.
- Barberet Guillaume
- Baron Rebecca M.
- Baronia Arvind K.
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- Barros Filipa M.
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- Bauer Philippe R.
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- Berghe Caroline
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- Ceylan Ilkay
- Chabanne Russell
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- Chen Kai
- Chena Ariel
- Cheng Meisy Perez
- Chiche Jean-Daniel
- Chouchene Imed
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- Grelon Fabien
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- Yuste Luis J.
- Zacharas Giannis A.
- Zacharowski Kai D.
- Zani Gianluca
- Zantua Kim S.
- Zazu Alan
- Zeggwagh Amine Ali
- Zein Salem Ould
- Zender Hervé O.
- Zhai Zhe
- Zhang Fan
- Zhang Xing Y.
- Zhao Huiying
- Zhao Jun
- Zhao Xian Y.
- Zheng Ruiqiang
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- Zhou Suming
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- Zhu Wei H.
- Zogheib Elie H.
- Ñamendys-Silva Silvio A.
- Ünal Necmettin
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- 01/01/2020
- Field of study
Publisher Copyright: © 2020 The Author(s). Copyright: Copyright 2020 Elsevier B.V., All rights reserved.Background: Concerns exist regarding the prevalence and impact of unnecessary oxygen use in patients with acute respiratory distress syndrome (ARDS). We examined this issue in patients with ARDS enrolled in the Large observational study to UNderstand the Global impact of Severe Acute respiratory FailurE (LUNG SAFE) study. Methods: In this secondary analysis of the LUNG SAFE study, we wished to determine the prevalence and the outcomes associated with hyperoxemia on day 1, sustained hyperoxemia, and excessive oxygen use in patients with early ARDS. Patients who fulfilled criteria of ARDS on day 1 and day 2 of acute hypoxemic respiratory failure were categorized based on the presence of hyperoxemia (PaO2 > 100 mmHg) on day 1, sustained (i.e., present on day 1 and day 2) hyperoxemia, or excessive oxygen use (FIO2 ≥ 0.60 during hyperoxemia). Results: Of 2005 patients that met the inclusion criteria, 131 (6.5%) were hypoxemic (PaO2 < 55 mmHg), 607 (30%) had hyperoxemia on day 1, and 250 (12%) had sustained hyperoxemia. Excess FIO2 use occurred in 400 (66%) out of 607 patients with hyperoxemia. Excess FIO2 use decreased from day 1 to day 2 of ARDS, with most hyperoxemic patients on day 2 receiving relatively low FIO2. Multivariate analyses found no independent relationship between day 1 hyperoxemia, sustained hyperoxemia, or excess FIO2 use and adverse clinical outcomes. Mortality was 42% in patients with excess FIO2 use, compared to 39% in a propensity-matched sample of normoxemic (PaO2 55-100 mmHg) patients (P = 0.47). Conclusions: Hyperoxemia and excess oxygen use are both prevalent in early ARDS but are most often non-sustained. No relationship was found between hyperoxemia or excessive oxygen use and patient outcome in this cohort. Trial registration: LUNG-SAFE is registered with ClinicalTrials.gov, NCT02010073publishersversionPeer reviewe
Pooled analysis of WHO Surgical Safety Checklist use and mortality after emergency laparotomy
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- Kaselis N
- Kaselis N
- Kasem E
- Kasem EA
- Kasputyte S
- Kaul A
- Kazanavicius D
- Kedar A
- Kefalidi E
- Keke KR
- Kelany M
- Kenibar A
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- Kennedy M
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- Kesinger M
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- Khairy H
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- Khatri C
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- Khedr AE-RH
- Khoja A
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- Khokha D
- Khokha V
- Khosla S
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- Khreishi R
- Khreishi R
- Khulu LB
- Khyrallh A
- Kiasat A
- Kidd T
- Kielty P
- Kim MY-H
- Kim S-H
- Kim SB
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- King R
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- Kirishnan A
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- Kjellin A
- Klappenbach R
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- Kobal BB
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- Kojo ATT
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- Kolosov A
- Komolafe O
- Kondrotas P
- Kong C
- Kong SN
- Kong V
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- Konstantina K
- Kontos M
- Kordorwu HEK
- Korkolis D
- Kosai NR
- Kosai NR
- Kose E
- Koskenvuo L
- Kostov E
- Kotb AHE-B
- Kotecha D
- Koto MZ
- Kotobi H
- Koumpa F
- Kouraklis G
- Kourdouli A
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- Kruspi S
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- Kyrou G
- Kyziridis D
- L'Heveder A
- La Brocca A
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- Labib P
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- Lakshmi HN
- Lam FY
- Lam JCY
- Lam WH
- Lamanna GL
- Lanau MIV
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- Lap G
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- Laurie K
- Lauzikas G
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- Lawal A
- Lawal AO
- Lawal T
- Lawal TA
- Lawani I
- Lawther R
- Lazzari G
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- Le VTH
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- Lecompte J-F
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- Lee F
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- Leusink A
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- Liew I
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- Lilford R
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- Lim G
- Lim H
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- Lim PJ
- Lim SJ
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- Lim SY
- Limache S
- Limaye N
- Limoges B
- Linares Delgado MSDFT
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- Livingston M
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- Macchitella Y
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- Macfarlane A
- Machaca M
- Machain Vega GM
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- Maciejec L
- Madiba T
- Madkor MH
- Madrid Barrientos LA
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- Manfreda S
- Mangan C
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- Mann T
- Mannu GS
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- Marret J-B
- Marroquin Rodriguez DE
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- Martian B-V
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- Mattar O
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- Mirza A
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- Mohamed B
- Mohamed F
- Mohamed I
- Mohamed M
- Mohamed MA
- Mohamed MAEH
- Mohamed MM
- Mohamed O
- Mohamed SR
- Mohameden A
- Mohammad A
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- Mohammed MMH
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- Montoya Botero JA
- Montrimaite M
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- Ruiz-Tovar J
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- Sadig N
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- Sahel S
- Saidbadr A
- Sakr A
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- Salah OM
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- Salma M
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- Salma U
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- Samalavicius NE
- Samaraweera DI
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- Samir A
- Samison LH
- Sammarco G
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- Samy S
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- Sandler S
- Sandoval Barrantes AM
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- Sanz S
- Sapci I
- Saqlain M
- Sarafilovic H
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- Scott M
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- Semeda N
- Semiz A
- Sena-Ruiz F
- Senanayake KJ
- Senanayake WSMKJ
- Setiawan J
- Seto DM
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- Sfougaris D
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- Shachykava T
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- Shahbaz AR
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- Shahine A
- Shahzad N
- Shaikh N
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- Shalabi A
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- Sharma A
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- Publication venue
- Publication date
- 15/10/2018
- Field of study
Background The World Health Organization (WHO) Surgical Safety Checklist has fostered safe practice for 10 years, yet its place in emergency surgery has not been assessed on a global scale. The aim of this study was to evaluate reported checklist use in emergency settings and examine the relationship with perioperative mortality in patients who had emergency laparotomy. Methods In two multinational cohort studies, adults undergoing emergency laparotomy were compared with those having elective gastrointestinal surgery. Relationships between reported checklist use and mortality were determined using multivariable logistic regression and bootstrapped simulation. Results Of 12 296 patients included from 76 countries, 4843 underwent emergency laparotomy. After adjusting for patient and disease factors, checklist use before emergency laparotomy was more common in countries with a high Human Development Index (HDI) (2455 of 2741, 89.6 per cent) compared with that in countries with a middle (753 of 1242, 60.6 per cent; odds ratio (OR) 0.17, 95 per cent c.i. 0.14 to 0.21, P <0001) or low (363 of 860, 422 per cent; OR 008, 007 to 010, P <0.001) HDI. Checklist use was less common in elective surgery than for emergency laparotomy in high-HDI countries (risk difference -94 (95 per cent c.i. -11.9 to -6.9) per cent; P <0001), but the relationship was reversed in low-HDI countries (+121 (+7.0 to +173) per cent; P <0001). In multivariable models, checklist use was associated with a lower 30-day perioperative mortality (OR 0.60, 0.50 to 073; P <0.001). The greatest absolute benefit was seen for emergency surgery in low- and middle-HDI countries. Conclusion Checklist use in emergency laparotomy was associated with a significantly lower perioperative mortality rate. Checklist use in low-HDI countries was half that in high-HDI countries.Peer reviewe
Global variation in anastomosis and end colostomy formation following left-sided colorectal resection
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- Chen Cy
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- Chidambaram S
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- Claire R
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- Clegg-Lampety Jn
- Clegg-Lamptey Jn
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- De Castro Segundo LCB
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- De La Medina AR
- de Leó
- De Leon MZP
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- Dell A
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- Depalma N
- Deputy M
- Der Y
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- Despotidis V
- Desta S
- Deutschmann P
- Devadasar Gh
- Dharamshi Ha
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- Di Martino N
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- Diab A
- Diaconescu B
- Diaconescu Ib
- Dias S
- Diaz-Jover P
- Diaz-Zorrilla C
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- Dickfos M
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- Dissanayake T
- Djivoh F
- Domini E
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- El-Ma&apos
- El-Rabaa S
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- Elashmawy M
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- Elbatahgy A
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- Eldin Mab
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- Elfeki H
- Elfiky M
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- Elkorashy Am
- Ellison Q
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- Elsherif Fa
- Elshobary M
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- Farhangmehr N
- Farinella E
- Farmakis K
- Farooq T
- Farouk Sam
- Farquharson A
- Farrugia A
- Farrugia A
- Faruq A
- Fathy Am
- Fattah Aa
- Fatuga A
- Faturoti O
- Faturoti O
- Faure A
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- Fawzy A
- Feidantsis T
- Felipe Co
- Fergany A
- Fergusson Sj
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- Fermani C
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- Firdouse M
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- Fowler Aj
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- Ibrahem Aa
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- Ibrahim A
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- Ibrahim I
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- Kong C
- Kong Sn
- Kong V
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- Konstantina K
- Kontos M
- Kordorwu Hek
- Korkolis D
- Kosai Nr
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- Kose E
- Koskenvuo L
- Kostov E
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- Kotecha D
- Koto Mz
- Kotobi H
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- Kouraklis G
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- Kretzmann H
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- Kwatiwani C
- Kwizera S
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- Kyriazanos I
- Kyrou G
- Kyziridis D
- Ló
- L&apos
- La Brocca A
- la Medina AR
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- la Medina AR
- Labib P
- Ladukas A
- Lafferty K
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- Lakhani Sm
- Lakshmi Hn
- Lam Fy
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- Lap G
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- Larsen Jw
- Lasek A
- Lasheen A
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- Latif A
- Laufer Lav
- Laugzemys E
- Laugzemys E
- Laurie K
- Lauzikas G
- Law Jh
- Lawal A
- Lawal Ao
- Lawal T
- Lawal Ta
- Lawani I
- Lawther R
- Lazzari G
- Lazzari V
- Leal A
- Leal M
- Lecompte Jf
- Lee C
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- Lee F
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- Lee Kc
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- Lee Tsb
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- Leone N
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- Leppä
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- Leung Kw
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- Leusink A
- Lewis J
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- Lilford R
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- Lim G
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- Loaloa Mr
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- Mabrouk M
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- Macchitella Y
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- Macfarlane A
- Machaca M
- Maciejec L
- Madiba T
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- Magdy A
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- Magee C
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- Marks E
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- Marshall Dc
- Martian Bv
- Martin A
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- Miranda C
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- Mirza A
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- Mistry A
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- Mogan Yp
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- Mohamed F
- Mohamed I
- Mohamed M
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- Mohamed Maeh
- Mohamed Mm
- Mohamed O
- Mohamed Sr
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- Monteleone M
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- Montes I
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- Montrimaite M
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- Moodley Y
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- Mostafa M
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- Moustafa A
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- Mshelbwala P
- Msiska N
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- Napolitano L
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- Narayanan R
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- Nepogodiev D
- Nepogodiev D
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- Nesaratnam A
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- Nevieraite V
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- Njokanma I
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- Nocito A
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- Nouh T
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- Novak A
- Nowers J
- Nsengimana Vjp
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- Ntirenganya F
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- Nurqistan Hd
- Nwabuoku E
- Nwinee V
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- Publication venue
- 'Wiley'
- Publication date
- 27/11/2018
- Field of study
Background
End colostomy rates following colorectal resection vary across institutions in high-income settings, being influenced by patient, disease, surgeon and system factors. This study aimed to assess global variation in end colostomy rates after left-sided colorectal resection.
Methods
This study comprised an analysis of GlobalSurg-1 and -2 international, prospective, observational cohort studies (2014, 2016), including consecutive adult patients undergoing elective or emergency left-sided colorectal resection within discrete 2-week windows. Countries were grouped into high-, middle- and low-income tertiles according to the United Nations Human Development Index (HDI). Factors associated with colostomy formation versus primary anastomosis were explored using a multilevel, multivariable logistic regression model.
Results
In total, 1635 patients from 242 hospitals in 57 countries undergoing left-sided colorectal resection were included: 113 (6·9 per cent) from low-HDI, 254 (15·5 per cent) from middle-HDI and 1268 (77·6 per cent) from high-HDI countries. There was a higher proportion of patients with perforated disease (57·5, 40·9 and 35·4 per cent; P < 0·001) and subsequent use of end colostomy (52·2, 24·8 and 18·9 per cent; P < 0·001) in low- compared with middle- and high-HDI settings. The association with colostomy use in low-HDI settings persisted (odds ratio (OR) 3·20, 95 per cent c.i. 1·35 to 7·57; P = 0·008) after risk adjustment for malignant disease (OR 2·34, 1·65 to 3·32; P < 0·001), emergency surgery (OR 4·08, 2·73 to 6·10; P < 0·001), time to operation at least 48 h (OR 1·99, 1·28 to 3·09; P = 0·002) and disease perforation (OR 4·00, 2·81 to 5·69; P < 0·001).
Conclusion
Global differences existed in the proportion of patients receiving end stomas after left-sided colorectal resection based on income, which went beyond case mix alone
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