Long-Term Variations in the Pixel-to-Pixel Variability of NOAA AVHRR SST Fields from 1982 to 2015

Sea surface temperature (SST) fields obtained from the series of space-borne five-channel Advanced Very High Resolution Radiometers (AVHRRs) provide the longest continuous time series of global SST available to date (1981–present). As a result, these data have been used for many studies and significant effort has been devoted to their careful calibration in an effort to provide a climate quality data record. However, little attention has been given to the local precision of the SST retrievals obtained from these instruments, which we refer to as the pixel-to-pixel (p2p) variability, a characteristic important in the ability to resolve structures such as ocean fronts characterized by small gradients in the SST field. In this study, the p2p variability is estimated for Level-2 SST fields obtained with the Pathfinder retrieval algorithm for AVHRRs on NOAA-07, 9, 11, 12 and 14-19. These estimates are stratified by year, season, day/night and along-scan/along-track. The overall variability ranges from 0.10 K to 0.21 K. For each satellite, the along-scan variability is between 10 and 20% smaller than the along-track variability (except for NOAA-16 nighttime for which it is approximately 30% smaller) and the summer and fall ss are between 10 and 15% smaller than the winter and spring ss. The differences between along-track and along-scan are attributed to the way in which the instrument has been calibrated. The seasonal differences result from the T4 - T5 term in the Pathfinder retrieval algorithm. This term is shown to be a major contributor to the p2p variability and it is shown that its impact could be substantially reduced without a deleterious effect on the overall p2p s of the resulting products by spatially averaging it as part of the retrieval process. The AVHRR/3s (NOAA-15 through 19) were found to be relatively stable with trends in the p2p variability of at most 0.015 K/decade

Alterations in vasodilator-stimulated phosphoprotein (VASP) phosphorylation: associations with asthmatic phenotype, airway inflammation and β\u3csub\u3e2\u3c/sub\u3e-agonist use

Background Vasodilator-stimulated phosphoprotein (VASP) mediates focal adhesion, actin filament binding and polymerization in a variety of cells, thereby inhibiting cell movement. Phosphorylation of VASP via cAMP and cGMP dependent protein kinases releases this brake on cell motility. Thus, phosphorylation of VASP may be necessary for epithelial cell repair of damage from allergen-induced inflammation. Two hypotheses were examined: (1) injury from segmental allergen challenge increases VASP phosphorylation in airway epithelium in asthmatic but not nonasthmatic normal subjects, (2) regular in vivo β2-agonist use increases VASP phosphorylation in asthmatic epithelium, altering cell adhesion. Methods Bronchial epithelium was obtained from asthmatic and non-asthmatic normal subjects before and after segmental allergen challenge, and after regularly inhaled albuterol, in three separate protocols. VASP phosphorylation was examined in Western blots of epithelial samples. DNA was obtained for β2-adrenergic receptor haplotype determination. Results Although VASP phosphorylation increased, it was not significantly greater after allergen challenge in asthmatics or normals. However, VASP phosphorylation in epithelium of nonasthmatic normal subjects was double that observed in asthmatic subjects, both at baseline and after challenge. Regularly inhaled albuterol significantly increased VASP phosphorylation in asthmatic subjects in both unchallenged and antigen challenged lung segment epithelium. There was also a significant increase in epithelial cells in the bronchoalveolar lavage of the unchallenged lung segment after regular inhalation of albuterol but not of placebo. The haplotypes of the β2-adrenergic receptor did not appear to associate with increased or decreased phosphorylation of VASP. Conclusion Decreased VASP phosphorylation was observed in epithelial cells of asthmatics compared to nonasthmatic normals, despite response to β-agonist. The decreased phosphorylation does not appear to be associated with a particular β2-adrenergic receptor haplotype. The observed decrease in VASP phosphorylation suggests greater inhibition of actin reorganization which is necessary for altering attachment and migration required during epithelial repair

Investigating the functionality of an OCT4-short response element in human induced pluripotent stem cells.

Pluripotent stem cells offer great therapeutic promise for personalized treatment platforms for numerous injuries, disorders, and diseases. Octamer-binding transcription factor 4 (OCT4) is a key regulatory gene maintaining pluripotency and self-renewal of mammalian cells. With site-specific integration for gene correction in cellular therapeutics, use of the OCT4 promoter may have advantages when expressing a suicide gene if pluripotency remains. However, the human OCT4 promoter region is 4 kb in size, limiting the capacity of therapeutic genes and other regulatory components for viral vectors, and decreasing the efficiency of homologous recombination. The purpose of this investigation was to characterize the functionality of a novel 967bp OCT4-short response element during pluripotency and to examine the OCT4 titer-dependent response during differentiation to human derivatives not expressing OCT4. Our findings demonstrate that the OCT4-short response element is active in pluripotency and this activity is in high correlation with transgene expression in vitro, and the OCT4-short response element is inactivated when pluripotent cells differentiate. These studies demonstrate that this shortened OCT4 regulatory element is functional and may be useful as part of an optimized safety component in a site-specific gene transferring system that could be used as an efficient and clinically applicable safety platform for gene transfer in cellular therapeutics

Variation in the cortical area map of C57BL/6J and DBA/2J inbred mice predicts strain identity

BACKGROUND: Recent discoveries suggest that arealization of the mammalian cortical sheet develops in a manner consonant with principles established for embryonic patterning of the body. Signaling centers release morphogens that determine regional growth and tissue identity by regulating regional expression of transcription factors. Research on mouse cortex has identified several candidate morphogens that affect anteroposterior or mediolateral cortical regionalization as well as mitogenesis. Inbred strains of laboratory mice can be exploited to study cortical area map formation if there are significant phenotypic differences with which to correlate gene polymorphism or expression data. Here we describe differences in the cortical area map of two commonly used inbred strains of laboratory mice, C57BL/6J and DBA/2J. Complete cortical hemispheres from adult mice were dissected and stained for the cytochrome oxidase enzyme in order to measure histochemically defined cortical areas. RESULTS: C57BL/6J has the larger neocortex, relatively larger primary visual cortex (V1), but relatively smaller posterior medial barrel subfield of the primary somatosensory cortex (PMBSF). The sample of C57BL/6J and DBA/2J mice can be discriminated with 90% accuracy on the basis of these three size dimensions. CONCLUSION: C57BL/6J and DBA/2J have markedly different cortical area maps, suggesting that inbred strains harbor enough phenotypic variation to encourage a forward genetic approach to understanding cortical development, complementing other approaches

The Importance of Disk Structure in Stalling Type I Migration

As planets form they tidally interact with their natal disks. Though the tidal perturbation induced by Earth and super-Earth mass planets is generally too weak to significantly modify the structure of the disk, the interaction is potentially strong enough to cause the planets to undergo rapid type I migration. This physical process may provide a source of short-period super-Earths, though it may also pose a challenge to the emergence and retention of cores on long-period orbits with sufficient mass to evolve into gas giants. Previous numerical simulations have shown that the type I migration rate sensitively depends upon the circumstellar disk's properties, particularly the temperature and surface density gradients. Here, we derive these structure parameters for 1) a self-consistent viscous-disk model based on a constant \alpha-prescription, 2) an irradiated disk model that takes into account heating due to the absorption of stellar photons, and 3) a layered-accretion disk model with variable \alpha-parameter. We show that in the inner viscously-heated regions of typical protostellar disks, the horseshoe and corotation torques of super-Earths can exceed their differential Lindblad torque and cause them to undergo outward migration. However, the temperature profile due to passive stellar irradiation causes type I migration to be inwards throughout much of the disk. For disks in which there is outwards migration, we show that location and the mass range of the "planet traps" depends on some uncertain assumptions adopted for these disk models. Competing physical effects may lead to dispersion in super-Earths' mass-period distribution.Comment: 12 pages, Submitted to Ap

Gut Microbiota-Produced Succinate Promotes C. difficile Infection after Antibiotic Treatment or Motility Disturbance

Clostridium difficile is a leading cause of antibiotic-associated diarrhea. The mechanisms underlying C. difficile expansion after microbiota disturbance are just emerging. We assessed the gene expression profile of C. difficile within the intestine of gnotobiotic mice to identify genes regulated in response to either dietary or microbiota compositional changes. In the presence of the gut symbiont Bacteroides thetaiotaomicron, C. difficile induces a pathway that metabolizes the microbiota fermentation end-product succinate to butyrate. The low concentration of succinate present in the microbiota of conventional mice is transiently elevated upon antibiotic treatment or chemically induced intestinal motility disturbance, and C. difficile exploits this succinate spike to expand in the perturbed intestine. A C. difficile mutant compromised in succinate utilization is at a competitive disadvantage during these perturbations. Understanding the metabolic mechanisms involved in microbiota-C. difficile interactions may help to identify approaches for the treatment and prevention of C. difficile-associated diseases

The DEEP Groth Strip Galaxy Redshift Survey. III. Redshift Catalog and Properties of Galaxies

The Deep Extragalactic Evolutionary Probe (DEEP) is a series of spectroscopic surveys of faint galaxies, targeted at the properties and clustering of galaxies at redshifts z ~ 1. We present the redshift catalog of the DEEP 1 GSS pilot phase of this project, a Keck/LRIS survey in the HST/WFPC2 Groth Survey Strip. The redshift catalog and data, including reduced spectra, are publicly available through a Web-accessible database. The catalog contains 658 secure galaxy redshifts with a median z=0.65, and shows large-scale structure walls to z = 1. We find a bimodal distribution in the galaxy color-magnitude diagram which persists to z = 1. A similar color division has been seen locally by the SDSS and to z ~ 1 by COMBO-17. For red galaxies, we find a reddening of only 0.11 mag from z ~ 0.8 to now, about half the color evolution measured by COMBO-17. We measure structural properties of the galaxies from the HST imaging, and find that the color division corresponds generally to a structural division. Most red galaxies, ~ 75%, are centrally concentrated, with a red bulge or spheroid, while blue galaxies usually have exponential profiles. However, there are two subclasses of red galaxies that are not bulge-dominated: edge-on disks and a second category which we term diffuse red galaxies (DIFRGs). The distant edge-on disks are similar in appearance and frequency to those at low redshift, but analogs of DIFRGs are rare among local red galaxies. DIFRGs have significant emission lines, indicating that they are reddened mainly by dust rather than age. The DIFRGs in our sample are all at z>0.64, suggesting that DIFRGs are more prevalent at high redshifts; they may be related to the dusty or irregular extremely red objects (EROs) beyond z>1.2 that have been found in deep K-selected surveys. (abridged)Comment: ApJ in press. 24 pages, 17 figures (12 color). The DEEP public database is available at http://saci.ucolick.org