1,142 research outputs found
GTP and Ca2+ Modulate the Inositol 1,4,5-Trisphosphate-Dependent Ca2+ Release in Streptolysin O-Permeabilized Bovine Adrenal Chromaffin Cells
The inositol 1,4,5-trisphosphate (IP3)-induced Ca2+ release was studied using streptolysin O-permeabilized bovine adrenal chromaffin cells. The IP3-induced Ca2+ release was followed by Ca2+ reuptake into intracellular compartments. The IP3-induced Ca2+ release diminished after sequential applications of the same amount of IP3. Addition of 20 μM GTP fully restored the sensitivity to IP3. Guanosine 5'-O-(3-thio)triphosphate (GTPγS) could not replace GTP but prevented the action of GTP. The effects of GTP and GTPγS were reversible. Neither GTP nor GTPγS induced release of Ca2+ in the absence of IP3. The amount of Ca2+ whose release was induced by IP3 depended on the free Ca2+ concentration of the medium. At 0.3 μM free Ca2+, a half-maximal Ca2+ release was elicited with ∼0.1 μM IP3. At 1 μM free Ca2+, no Ca2+ release was observed with 0.1 μM IP3; at this Ca2+ concentration, higher concentrations of IP3 (0.25 μM) were required to evoke Ca2+ release. At 8 μM free Ca2+, even 0.25 μM IP3 failed to induce release of Ca2+ from the store. The IP3-induced Ca2+ release at constant low (0.2 μM) free Ca2+ concentrations correlated directly with the amount of stored Ca2+. Depending on the filling state of the intracellular compartment, 1 mol of IP3 induced release of between 5 and 30 mol of Ca2+
Large-Scale Agile Transformation: A Case Study of Transforming Business, Development and Operations
Today, product development organizations are adopting agile methods in units outside the software development unit, such as in sales, market, legal, operations working with the customer. This broader adoption of agile methods has been labeled large-scale agile transformation and is considered a particular type of organizational change, originating in the software development units. So far, there is little research-based advice on conducting such transformations. Aiming to contribute towards providing relevant research advice on large-scale agile transformation, we apply a research-based framework for evaluating organizational agility on a product development program in a maritime service provider organization. We found that doing a large-scale agile transformation involves many significant challenges, such as having a shared understanding of the problem, getting access to users, and getting commitment to change that needs to be done. In order to overcome such challenges, we discuss the need for a holistic and integrated approach to agile transformation involving all the units linked to software development.publishedVersio
Comparative Modelling of the Spectra of Cool Giants
Our ability to extract information from the spectra of stars depends on
reliable models of stellar atmospheres and appropriate techniques for spectral
synthesis. Various model codes and strategies for the analysis of stellar
spectra are available today. We aim to compare the results of deriving stellar
parameters using different atmosphere models and different analysis strategies.
The focus is set on high-resolution spectroscopy of cool giant stars. Spectra
representing four cool giant stars were made available to various groups and
individuals working in the area of spectral synthesis, asking them to derive
stellar parameters from the data provided. The results were discussed at a
workshop in Vienna in 2010. Most of the major codes currently used in the
astronomical community for analyses of stellar spectra were included in this
experiment. We present the results from the different groups, as well as an
additional experiment comparing the synthetic spectra produced by various codes
for a given set of stellar parameters. Similarities and differences of the
results are discussed. Several valid approaches to analyze a given spectrum of
a star result in quite a wide range of solutions. The main causes for the
differences in parameters derived by different groups seem to lie in the
physical input data and in the details of the analysis method. This clearly
shows how far from a definitive abundance analysis we still are.Comment: accepted for publication in A&A. This version includes also the
online tables. Reference spectra will later be available via the CD
Requirements modelling and formal analysis using graph operations
The increasing complexity of enterprise systems requires a more advanced
analysis of the representation of services expected than is currently possible.
Consequently, the specification stage, which could be facilitated by formal
verification, becomes very important to the system life-cycle. This paper presents
a formal modelling approach, which may be used in order to better represent
the reality of the system and to verify the awaited or existing system’s properties,
taking into account the environmental characteristics. For that, we firstly propose
a formalization process based upon properties specification, and secondly we
use Conceptual Graphs operations to develop reasoning mechanisms of verifying
requirements statements. The graphic visualization of these reasoning enables us
to correctly capture the system specifications by making it easier to determine if
desired properties hold. It is applied to the field of Enterprise modelling
Detection of iron deficiency in children with Down syndrome
Purpose Current American Academy of Pediatrics guidelines for children with Down syndrome (DS) recommend a complete blood count (CBC) at birth and hemoglobin annually to screen for iron deficiency (ID) and ID anemia (IDA) in low-risk children. We aimed to determine if macrocytosis masks the diagnosis of ID/IDA and to evaluate the utility of biochemical and red blood cell indices for detecting ID/IDA in DS. Methods We reviewed data from 856 individuals from five DS specialty clinics. Data included hemoglobin, mean corpuscular volume, red cell distribution width (RDW), percent transferrin saturation (TS), ferritin, and c-reactive protein. Receiver operating characteristic curves were calculated. Results Macrocytosis was found in 32% of the sample. If hemoglobin alone was used for screening, all individuals with IDA would have been identified, but ID would have been missed in all subjects. RDW had the highest discriminability of any single test for ID/IDA. The combination of RDW with ferritin or TS led to 100% sensitivity, and RDW combined with ferritin showed the highest discriminability for ID/IDA. Conclusion We provide evidence to support that a CBC and ferritin be obtained routinely for children over 1 year old with DS rather than hemoglobin alone for detection of ID
The Gaia-ESO Survey: the most metal-poor stars in the Galactic bulge
We present the first results of the EMBLA survey (Extremely Metal-poor BuLge
stars with AAOmega), aimed at finding metal-poor stars in the Milky Way bulge,
where the oldest stars should now preferentially reside. EMBLA utilises
SkyMapper photometry to pre-select metal-poor candidates, which are
subsequently confirmed using AAOmega spectroscopy. We describe the discovery
and analysis of four bulge giants with -2.72<=[Fe/H]<=-2.48, the lowest
metallicity bulge stars studied with high-resolution spectroscopy to date.
Using FLAMES/UVES spectra through the Gaia-ESO Survey we have derived
abundances of twelve elements. Given the uncertainties, we find a chemical
similarity between these bulge stars and halo stars of the same metallicity,
although the abundance scatter may be larger, with some of the stars showing
unusual [{\alpha}/Fe] ratios.Comment: 7 pages, 5 figures. Accepted for publication by MNRA
The primordial binary population II: Recovering the binary population for intermediate mass stars in Sco OB2
We characterize the binary population in the young and nearby OB association
Scorpius OB2 using available observations of visual, spectroscopic, and
astrometric binaries with intermediate-mass primaries. We take into account
observational biases by comparing the observations with simulated observations
of model associations. The available data indicate a large binary fraction (>
70% with 3sigma confidence), with a large probability that all intermediate
mass stars in Sco OB2 are part of a binary system. The binary systems have a
mass ratio distribution of the form f(q) ~ q^-0.4. Sco OB2 has a semi-major
axis distribution of the form f(log a) ~ constant (Opik's law), in the range
5-5e6 Rsun. The log-normal period distribution of Duquennoy & Mayor results in
too few spectroscopic binaries, even if the model binary fraction is 100%. Sco
OB2 is a young association with a low stellar density; its current population
is expected to be very similar to the primordial population. The fact that
practically all stars in Sco OB2 are part of a binary (or multiple) system
demonstrates that multiplicity is a fundamental factor in the star formation
process, at least for intermediate mass stars.Comment: 36 pages, 11 figures, accepted by A&
The Gaia-ESO Survey: Chromospheric Emission, Accretion Properties, and Rotation in Velorum and Chamaeleon I
We use the fundamental parameters delivered by the GES consortium in the
first internal data release to select the members of Vel and Cha I
among the UVES and GIRAFFE spectroscopic observations. A total of 140
Vel members and 74 Cha I members were studied. We calculated stellar
luminosities through spectral energy distributions, while stellar masses were
derived by comparison with evolutionary tracks. The spectral subtraction of
low-activity and slowly rotating templates, which are rotationally broadened to
match the of the targets, enabled us to measure the equivalent widths
(EWs) and the fluxes in the H and H lines. The H line
was also used for identifying accreting objects and for evaluating the mass
accretion rate (). The distribution of for the
members of Vel displays a peak at about 10 km s with a tail
toward faster rotators. There is also some indication of a different
distribution for the members of its two kinematical populations. Only a handful
of stars in Vel display signatures of accretion, while many more
accretors were detected in the younger Cha~I. Accreting and active stars occupy
two different regions in a -flux diagram and we propose a
criterion for distinguishing them. We derive in the ranges
-yr and -yr
for Vel and Cha I accretors, respectively. We find less scatter in the
relation derived through the H EWs, when
compared to the H diagnostics, in agreement with other authors
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Reactor physics methods, models, and applications used to support the conceptual design of the Advanced Neutron Source
This report summarizes the neutronics analysis performed during 1991 and 1992 in support of characterization of the conceptual design of the Advanced Neutron Source (ANS). The methods used in the analysis, parametric studies, and key results supporting the design and safety evaluations of the conceptual design are presented. The analysis approach used during the conceptual design phase followed the same approach used in early ANS evaluations: (1) a strong reliance on Monte Carlo theory for beginning-of-cycle reactor performance calculations and (2) a reliance on few-group diffusion theory for reactor fuel cycle analysis and for evaluation of reactor performance at specific time steps over the fuel cycle. The Monte Carlo analysis was carried out using the MCNP continuous-energy code, and the few- group diffusion theory calculations were performed using the VENTURE and PDQ code systems. The MCNP code was used primarily for its capability to model the reflector components in realistic geometries as well as the inherent circumvention of cross-section processing requirements and use of energy-collapsed cross sections. The MCNP code was used for evaluations of reflector component reactivity effects and of heat loads in these components. The code was also used as a benchmark comparison against the diffusion-theory estimates of key reactor parameters such as region fluxes, control rod worths, reactivity coefficients, and material worths. The VENTURE and PDQ codes were used to provide independent evaluations of burnup effects, power distributions, and small perturbation worths. The performance and safety calculations performed over the subject time period are summarized, and key results are provided. The key results include flux and power distributions over the fuel cycle, silicon production rates, fuel burnup rates, component reactivities, control rod worths, component heat loads, shutdown reactivity margins, reactivity coefficients, and isotope production rates
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