Tracking Lumbar Vertebrae in Digital Videofluoroscopic Video Automatically

Low back pain becomes one of the significant problem in the industrialized world. Efficient and effective spinal motion analysis is required to understand low back pain and to aid the diagnosis. Videofluoroscopy provides a cost effective way for such analysis. However, common approaches are tedious and time consuming due to the low quality of the images. Physicians have to extract the vertebrae manually in most cases and thus continuous motion analysis is hardly achieved. In this paper, we propose a system which can perform automatic vertebrae segmentation and tracking. Operators need to define exact location of landmarks in the first frame only. The proposed system will continuously learn the texture pattern along the edge and the dynamics of the vertebrae in the remaining frames. The system can estimate the location of the vertebrae based on the learnt texture and dynamics throughout the sequence. Experimental results show that the proposed system can segment vertebrae from videofluoroscopic images automatically and accurately. © Springer-Verlag 2004.postprintThe International Workshop on Medical Imaging and Augmented Reality (MIAR 2004), Beijing, China, 19-20 August 2004. In Lecture Notes in Computer Science, 2004, v. 3150, p. 154-16

Acupuncture for insomnia

Although conventional non-pharmacological and pharmacological treatments for insomnia are effective in many people, alternative therapies such as acupuncture are widely practised. However, it remains unclear whether current evidence is rigorous enough to support acupuncture for the treatment of insomnia. To determine the efficacy and safety of acupuncture for insomnia. We searched the Cochrane Central Register of Controlled Trials (CENTRAL), MEDLINE, EMBASE, PsycINFO, Dissertation Abstracts International, CINAHL, AMED, the Traditional Chinese Medical Literature Analysis and Retrieval System (TCMLARS), the World Health Organization (WHO) Trials Portal (ICTRP) and relevant specialised registers of the Cochrane Collaboration in October 2011. We screened reference lists of all eligible reports and contacted trial authors and experts in the field. Randomised controlled trials evaluating any form of acupuncture for insomnia. They compared acupuncture with/without additional treatment against placebo or sham or no treatment or same additional treatment. We excluded trials that compared different acupuncture methods or acupuncture against other treatments. Two review authors independently extracted data and assessed risk of bias. We used odds ratio (OR) and mean difference for binary and continuous outcomes respectively. We combined data in meta-analyses where appropriate. Thirty-three trials were included. They recruited 2293 participants with insomnia, aged 15 to 98 years, some with medical conditions contributing to insomnia (stroke, end-stage renal disease, perimenopause, pregnancy, psychiatric diseases). They evaluated needle acupuncture, electroacupuncture, acupressure or magnetic acupressure.Compared with no treatment (two studies, 280 participants) or sham/placebo (two studies, 112 participants), acupressure resulted in more people with improvement in sleep quality (compared to no treatment: OR 13.08, 95% confidence interval (CI) 1.79 to 95.59; compared to sham/placebo: OR 6.62, 95% CI 1.78 to 24.55). However, when assuming that dropouts had a worse outcome in sensitivity analysis the beneficial effect of acupuncture was inconclusive. Compared with other treatment alone, acupuncture as an adjunct to other treatment might marginally increase the proportion of people with improved sleep quality (13 studies, 883 participants, OR 3.08, 95% CI 1.93 to 4.90). On subgroup analysis, only needle acupuncture but not electroacupuncture showed benefits. All trials had high risk of bias and were heterogeneous in the definition of insomnia, participant characteristics, acupoints and treatment regimen. The effect sizes were generally small with wide confidence intervals. Publication bias was likely present. Adverse effects were rarely reported and they were minor. Due to poor methodological quality, high levels of heterogeneity and publication bias, the current evidence is not sufficiently rigorous to support or refute acupuncture for treating insomnia. Larger high-quality clinical trials are required.link_to_subscribed_fulltex

Report of 2 fatal cases of adult necrotizing fasciitis and toxic shock syndrome caused by Streptococcus agalactiae.

We describe 2 cases of fatal necrotizing fasciitis and toxic shock syndrome caused by Streptococcus agalactiae-a rare entity that has been reported in only 9 patients-in 2 nonpregnant adults.published_or_final_versio

A correlation study between in-brace correction, compliance to spinal orthosis and health-related quality of life of patients with Adolescent Idiopathic Scoliosis

BACKGROUND: It has been proposed that in-brace correction is the best guideline for prediction of the results of brace treatment for patients with Adolescent Idiopathic Scoliosis (AIS). However, bracing may be a stressful experience for patients and bracing non-compliance could be psychologically related. The purpose of this study was to assess the correlation between brace compliance, in-brace correction and QoL of patients with AIS. METHODS: Fifty-five patients with a diagnosis of AIS were recruited. All were female and aged 10 years or above when a brace was prescribed, none had undergone prior treatment, and all had a Risser sign of 0–2 and a Cobb angle of 25-40°. The patients were examined in three consecutive visits with 4 to 6 months between each visit. The Chinese translated Trunk Appearance Perception Scale (TAPS), the Chinese translated Brace Questionnaires (BrQ) and the Chinese translated SRS-22 Questionnaires were used in the study. The in-brace Cobb angle, vertebral rotation and trunk listing were also measured. Patients’ compliance, in-brace correction and patients’ QoL were assessed. To identify the relationship among these three areas, logistic regression model and generalized linear model were used. RESULT: For the compliance measure, a significant difference (p = 0.008) was detected on TAPS mean score difference between Visit 1 and Visit 2 in the least compliant group (0–8 hours) and the most compliant group (17–23 hours). In addition, a significant difference (p = 0.000) was detected on BrQ mean score difference between Visit 2 and Visit 3 in the least compliant group (0–8 hours) and the most compliant group (17–23 hours). For the orthosis effectiveness measure, no significant difference was detected between the three groups of bracing hours (0–8 hours, 9–16 hours, 17–23 hours) on in-brace correction (below 40% and 40% or above). For the QoL measure, no significant difference was detected between the two different in-brace correction groups (below 40% and 40% or above) on QoL as reflected by the TAPS, BrQ and SRS-22r mean scores. CONCLUSION: The results showed a positive relationship between patients’ brace wear compliance and patients’ QoL. Poor compliance would cause a lower QoL

Beam Squint in Ultra-wideband mmWave Systems: RF Lens Array vs. Phase-Shifter-Based Array

In this article, we discuss the potential of radio frequency (RF) lens for ultra-wideband millimeter-wave (mmWave) systems. In terms of the beam squint, we compare the proposed RF lens antenna with the phase shifter-based array for hybrid beamforming. To reduce the complexities for fully digital beamforming, researchers have come up with RF lens-based hybrid beamforming. The use of mmWave systems, however, causes an increase in bandwidth, which gives rise to the beam squint phenomenon. We first find the causative factors for beam squint in the dielectric RF lens antenna. Based on the beamforming gain at each frequency, we verify that, in a specific situation, RF lens can be free of the beam squint effect. We use 3D electromagnetic analysis software to numerically interpret the beam squint of each antenna type. Based on the results, we present the degraded spectral efficiency by system-level simulations with 3D indoor ray tracing. Finally, to verify our analysis, we fabricate an actual RF lens antenna and demonstrate the real performance using a mmWave, NI PXIe, software-defined radio system

Roboteye technology for thermal target tracking using predictive control

© ISARC 2018 - 35th International Symposium on Automation and Robotics in Construction and International AEC/FM Hackathon: The Future of Building Things. All rights reserved. Thermal cameras are widely used in the fatigue analysis of mechanical structures using the thermoelastic effect. Nevertheless, such analysis is hampered due to blurry images resulting from the motion of structure-under-test. To address the issue this paper presents a system that utilizes robotic vision and predictive control. The system comprises of a thermal camera, a vision camera, a RobotEye, and a fiducial detection system. A marker is attached to a thermal target in order to estimate its position and orientation using the proposed detection system. To predict the future position of the thermal moving object, a Kalman filter is used. Finally, the Model Predictive Control (MPC) approach is applied to generate commands for the robot to follow the target. Results of the tracking by MPC are included in this paper along with the performance evaluation of the whole system. The evaluation clearly shows the improvement in the tracking performance of the development for thermal structural analysis

Dioxiranes generated in situ from pyruvates and oxone as environmentally friendly oxidizing agents for disinfection

Dioxiranes generated in situ from pyruvates (α-keto esters) and Oxone have been found to be environmentally friendly oxidizing agents for disinfection. These oxidizing agents were highly effective for destruction of various strains of bacteria, fungi, and bacterial endospores in a wide temperature range with exceptional stability. Notably, by using an aqueous solution of methyl pyruvate (1a) and Oxone/NaHCO3, complete destruction of bacteria such as Staphylococcus aureusand fungus Penicillium corylophilum was achieved within 5 min at 20 °C at neutral pH. Highly chemical-resistant bacterial endospores of Bacillus cereus could also be destroyed. The high antibacterial activity of 1a could be attributed to its strong electron-withdrawing α-ester group. © 2006 American Chemical Society.postprin

Mothers' preference and willingness to pay for human papillomavirus vaccination for their daughters: a discrete choice experiment

In Hong Kong, cervical cancer is the seventh commonest cancer among females and accounts for about 3.6% of all new cancer cases. To reduce the disease burden, a cervical cancer screening programme was launched in 2004 and two vaccines were introduced in 2006. However, HPV vaccination is not included in the government immunisation schedule and is largely taken up in private clinics opportunistically. The uptake rate of HPV vaccination among adolescent girls in Hong Kong is low (2.1%-9.1%).1,2 The success of a HPV vaccination programme largely depends on the local stakeholders’ attitude towards risks and benefits of vaccination. Our study used a discrete choice experiment to elicit Hong Kong mothers’ preference and willingness to pay (WTP) for HPV vaccination for their daughters

Transcriptome-Guided Functional Analyses Reveal Novel Biological Properties and Regulatory Hierarchy of Human Embryonic Stem Cell-Derived Ventricular Cardiomyocytes Crucial for Maturation

Human (h) embryonic stem cells (ESC) represent an unlimited source of cardiomyocytes (CMs); however, these differentiated cells are immature. Thus far, gene profiling studies have been performed with non-purified or non-chamber specific CMs. Here we took a combinatorial approach of using systems biology to guide functional discoveries of novel biological properties of purified hESC-derived ventricular (V) CMs. We profiled the transcriptomes of hESCs, hESC-, fetal (hF) and adult (hA) VCMs, and showed that hESC-VCMs displayed a unique transcriptomic signature. Not only did a detailed comparison between hESC-VCMs and hF-VCMs confirm known expression changes in metabolic and contractile genes, it further revealed novel differences in genes associated with reactive oxygen species (ROS) metabolism, migration and cell cycle, as well as potassium and calcium ion transport. Following these guides, we functionally confirmed that hESC-VCMs expressed IKATP with immature properties, and were accordingly vulnerable to hypoxia/reoxygenation-induced apoptosis. For mechanistic insights, our coexpression and promoter analyses uncovered a novel transcriptional hierarchy involving select transcription factors (GATA4, HAND1, NKX2.5, PPARGC1A and TCF8), and genes involved in contraction, calcium homeostasis and metabolism. These data highlight novel expression and functional differences between hESC-VCMs and their fetal counterparts, and offer insights into the underlying cell developmental state. These findings may lead to mechanism-based methods for in vitro driven maturation. © 2013 Poon et al.published_or_final_versio

SARS-CoV-2-specific immunoglobulin Y antibodies are protective in infected mice

Safe, passive immunization methods are required against severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) and its variants. Immunization of chickens with antigen is known to induce specific IgY antibodies concentrated in the egg yolk and has a good safety profile, high yield of IgY per egg, can be topically applied, not requiring parenteral delivery. Our data provide the first evidence of the prophylactic efficacy of Immunoglobulin Y antibodies against SARS-CoV-2 in mice. Lohmann hens were injected with recombinant SARS-CoV-2 RBD protein; IgY-Abs were extracted from the eggs and characterized using SDS-PAGE. Antiviral activity was evaluated using plaque reduction neutralization tests. In additional experiments, IgY-RBD efficacy was examined in mice sensitized to SARS-CoV-2 infection by transduction with Ad5-hACE2 (mild disease) or by using mouse-adapted virus (severe disease). In both cases, prophylactic intranasal administration of IgY-Abs reduced SARS-CoV-2 replication, and reduced morbidity, inflammatory cell infiltration, hemorrhage, and edema in the lungs and increased survival compared to control groups that received non-specific IgY-Abs. These results indicate that further evaluation of IgY-RBD antibodies in humans is warranted