The Lawrence Berkeley Laboratory geothermal program in northern Nevada

The Lawrence Berkeley Laboratory's geothermal program began with consideration of regions where fluids in the temperature range of 150 to 230 C may be economically accessible. Three valleys, located in an area of high regional heat flow in north central Nevada, were selected for geological, geophysical, and geochemical field studies. The objective of these ongoing field activities is to select a site for a 10-MW demonstration plant. Field activities (which started in September 1973) are described. A parallel effort has been directed toward the conceptual design of a 10-MW isobutane binary plant which is planned for construction at the selected site. Design details of the plant are described. Project schedule with milestones is shown together with a cost summary of the project

Human Vault Nanoparticle Targeted Delivery of Antiretroviral Drugs to Inhibit Human Immunodeficiency Virus Type 1 Infection.

"Vaults" are ubiquitously expressed endogenous ribonucleoprotein nanoparticles with potential utility for targeted drug delivery. Here, we show that recombinant human vault nanoparticles are readily engulfed by certain key human peripheral blood mononuclear cells (PBMC), predominately dendritic cells, monocytes/macrophages, and activated T cells. As these cell types are the primary targets for human immunodeficiency virus type 1 (HIV-1) infection, we examined the utility of recombinant human vaults for targeted delivery of antiretroviral drugs. We chemically modified three different antiretroviral drugs, zidovudine, tenofovir, and elvitegravir, for direct conjugation to vaults. Tested in infection assays, drug-conjugated vaults inhibited HIV-1 infection of PBMC with equivalent activity to free drugs, indicating vault delivery and drug release in the cytoplasm of HIV-1-susceptible cells. The ability to deliver functional drugs via vault nanoparticle conjugates suggests their potential utility for targeted drug delivery against HIV-1

Algebraic Quantum Theory on Manifolds: A Haag-Kastler Setting for Quantum Geometry

Motivated by the invariance of current representations of quantum gravity under diffeomorphisms much more general than isometries, the Haag-Kastler setting is extended to manifolds without metric background structure. First, the causal structure on a differentiable manifold M of arbitrary dimension (d+1>2) can be defined in purely topological terms, via cones (C-causality). Then, the general structure of a net of C*-algebras on a manifold M and its causal properties required for an algebraic quantum field theory can be described as an extension of the Haag-Kastler axiomatic framework. An important application is given with quantum geometry on a spatial slice within the causally exterior region of a topological horizon H, resulting in a net of Weyl algebras for states with an infinite number of intersection points of edges and transversal (d-1)-faces within any neighbourhood of the spatial boundary S^2.Comment: 15 pages, Latex; v2: several corrections, in particular in def. 1 and in sec.

Microlocal analysis of quantum fields on curved spacetimes: Analytic wavefront sets and Reeh-Schlieder theorems

We show in this article that the Reeh-Schlieder property holds for states of quantum fields on real analytic spacetimes if they satisfy an analytic microlocal spectrum condition. This result holds in the setting of general quantum field theory, i.e. without assuming the quantum field to obey a specific equation of motion. Moreover, quasifree states of the Klein-Gordon field are further investigated in this work and the (analytic) microlocal spectrum condition is shown to be equivalent to simpler conditions. We also prove that any quasifree ground- or KMS-state of the Klein-Gordon field on a stationary real analytic spacetime fulfills the analytic microlocal spectrum condition.Comment: 31 pages, latex2

Lesion environments direct transplanted neural progenitors towards a wound repair astroglial phenotype in mice.

Neural progenitor cells (NPC) represent potential cell transplantation therapies for CNS injuries. To understand how lesion environments influence transplanted NPC fate in vivo, we derived NPC expressing a ribosomal protein-hemagglutinin tag (RiboTag) for transcriptional profiling of transplanted NPC. Here, we show that NPC grafted into uninjured mouse CNS generate cells that are transcriptionally similar to healthy astrocytes and oligodendrocyte lineages. In striking contrast, NPC transplanted into subacute CNS lesions after stroke or spinal cord injury in mice generate cells that share transcriptional, morphological and functional features with newly proliferated host astroglia that restrict inflammation and fibrosis and isolate lesions from adjacent viable neural tissue. Our findings reveal overlapping differentiation potentials of grafted NPC and proliferating host astrocytes; and show that in the absence of other interventions, non-cell autonomous cues in subacute CNS lesions direct the differentiation of grafted NPC towards a naturally occurring wound repair astroglial phenotype

Natural climate solutions

Our thanks for inputs by L. Almond, A. Baccini, A. Bowman, S. CookPatton, J. Evans, K. Holl, R. Lalasz, A. Nassikas, M. Spalding, M. Wolosin, and expert elicitation respondents. Our thanks for datasets developed by the Hansen lab and the NESCent grasslands working group (C. Lehmann, D. Griffith, T. M. Anderson, D. J. Beerling, W. Bond, E. Denton, E. Edwards, E. Forrestel, D. Fox, W. Hoffmann, R. Hyde, T. Kluyver, L. Mucina, B. Passey, S. Pau, J. Ratnam, N. Salamin, B. Santini, K. Simpson, M. Smith, B. Spriggs, C. Still, C. Strömberg, and C. P. Osborne). This study was made possible by funding from the Doris Duke Charitable Foundation. Woodbury was supported in part by USDA-NIFA Project 2011-67003-30205 Data deposition: A global spatial dataset of reforestation opportunities has been deposited on Zenodo (https://zenodo.org/record/883444). This article contains supporting information online at www.pnas.org/lookup/suppl/doi:10.1073/pnas.1710465114/-/DCSupplemental.Peer reviewedPublisher PD

Modular Localization of Massive Particles with "Any" Spin in d=2+1

We discuss a concept of particle localization which is motivated from quantum field theory, and has been proposed by Brunetti, Guido and Longo and by Schroer. It endows the single particle Hilbert space with a family of real subspaces indexed by the space-time regions, with certain specific properties reflecting the principles of locality and covariance. We show by construction that such a localization structure exists also in the case of massive anyons in d=2+1, i.e. for particles with positive mass and with arbitrary spin s in the reals. The construction is completely intrinsic to the corresponding ray representation of the (proper orthochronous) Poincare group. Our result is of particular interest since there are no free fields for anyons, which would fix a localization structure in a straightforward way. We present explicit formulas for the real subspaces, expected to turn out useful for the construction of a quantum field theory for anyons. In accord with well-known results, only localization in string-like, instead of point-like or bounded, regions is achieved. We also prove a single-particle PCT theorem, exhibiting a PCT operator which acts geometrically correctly on the family of real subspaces