Australia's coastal waters are rich in Indigenous cultural heritage, but it remains hidden and under threat

[Extract] In two new studies published in Australian Archaeology, we outline approaches to help us better understand and manage Indigenous underwater cultural heritage. Through a two-pronged approach at both the local and regional level, we review big data to predict the location of sites. We also put boots on the ground and divers in the water to find and record them

Beneath the Top End: a regional assessment of submerged archaeological potential in the Northern Territory, Australia

Regional-scale assessments have proven to be invaluable frameworks for research, public engagement and management of submerged archaeological landscapes. Regional-scale approaches have been implemented internationally through a variety of academic or strategic studies. Such studies represent a much-needed next step towards subregional and site-level prospection to support management, engagement and mitigation of the impacts of offshore development. However, these regional studies are largely absent in Australia. In this article, we build on the recent discovery of submerged archaeological sites in Western Australia and produce a novel regional-scale assessment of submerged archaeological and cultural landscape potential in the coastal and island regions of the Northern Territory. This area is of special significance in the peopling of Australia, containing some of the oldest dated archaeological evidence. We collate and synthesise regional data related to sea-level change, ethnography (e.g. oral traditions), geomorphology, and archaeology, also taking account of logistics and existing data availability to identify prospective areas for further study. We highlight the need for a coordinated national program of regional baseline studies to address a legacy of under-representation of submerged landscapes and provide vital baseline data for a wide spectrum of stakeholders, including researchers, policy makers, environmental and heritage managers, developers and Traditional Owners

Aboriginal artefacts on the continental shelf reveal ancient drowned cultural landscapes in northwest Australia

This article reports Australia’s first confirmed ancient underwater archaeological sites from the continental shelf, located off the Murujuga coastline in north-western Australia. Details on two underwater sites are reported: Cape Bruguieres, comprising > 260 recorded lithic artefacts at depths down to −2.4 m below sea level, and Flying Foam Passage where the find spot is associated with a submerged freshwater spring at −14 m. The sites were discovered through a purposeful research strategy designed to identify underwater targets, using an iterative process incorporating a variety of aerial and underwater remote sensing techniques and diver investigation within a predictive framework to map the submerged landscape within a depth range of 0–20 m. The condition and context of the lithic artefacts are analysed in order to unravel their depositional and taphonomic history and to corroborate their in situ position on a pre-inundation land surface, taking account of known geomorphological and climatic processes including cyclone activity that could have caused displacement and transportation from adjacent coasts. Geomorphological data and radiometric dates establish the chronological limits of the sites and demonstrate that they cannot be later than 7000 cal BP and 8500 cal BP respectively, based on the dates when they were finally submerged by sea-level rise. Comparison of underwater and onshore lithic assemblages shows differences that are consistent with this chronological interpretation. This article sets a foundation for the research strategies and technologies needed to identify archaeological targets at greater depth on the Australian continental shelf and elsewhere, building on the results presented. Emphasis is also placed on the need for legislation to better protect and manage underwater cultural heritage on the 2 million square kilometres of drowned landscapes that were once available for occupation in Australia, and where a major part of its human history must lie waiting to be discovered

Stone artefacts on the seabed at a submerged freshwater spring confirm a drowned cultural landscape in Murujuga, Western Australia

We report the discovery and identification of five ancient stone artefacts associated with a submerged freshwater spring at the underwater archaeological site WH1 in Murujuga (Dampier Archipelago), Western Australia. A limiting date applied to the site based on timing of inundation suggests it was occupied in the Late Pleistocene or Early Holocene. The site is situated well below the intertidal zone having been recorded at 14 m depth in Flying Foam Passage. This discovery highlights the high potential of these submerged springs as archaeological survey targets. We discuss results of a recent survey that expands the number of confirmed artefacts located at WH1 and the geomorphological context in a large calcareous depression associated with a freshwater source. This study demonstrates how submerged landscape research using a suite of technologies can reveal archaeological assemblages in this tropical geomorphological environment, and that adapted techniques could be applied to other tropical conditions such as mangrove coasts, large deltaic plains, or reef-building environments. There are likely thousands of drowned archaeological sites on the continental shelf of the tropics, extending from the intertidal zone to the lowest point of the culturally occupied landmass, at approximately 130 m below modern sea level

Effect of Hydrocortisone on Mortality and Organ Support in Patients With Severe COVID-19: The REMAP-CAP COVID-19 Corticosteroid Domain Randomized Clinical Trial.

Importance: Evidence regarding corticosteroid use for severe coronavirus disease 2019 (COVID-19) is limited. Objective: To determine whether hydrocortisone improves outcome for patients with severe COVID-19. Design, Setting, and Participants: An ongoing adaptive platform trial testing multiple interventions within multiple therapeutic domains, for example, antiviral agents, corticosteroids, or immunoglobulin. Between March 9 and June 17, 2020, 614 adult patients with suspected or confirmed COVID-19 were enrolled and randomized within at least 1 domain following admission to an intensive care unit (ICU) for respiratory or cardiovascular organ support at 121 sites in 8 countries. Of these, 403 were randomized to open-label interventions within the corticosteroid domain. The domain was halted after results from another trial were released. Follow-up ended August 12, 2020. Interventions: The corticosteroid domain randomized participants to a fixed 7-day course of intravenous hydrocortisone (50 mg or 100 mg every 6 hours) (n = 143), a shock-dependent course (50 mg every 6 hours when shock was clinically evident) (n = 152), or no hydrocortisone (n = 108). Main Outcomes and Measures: The primary end point was organ support-free days (days alive and free of ICU-based respiratory or cardiovascular support) within 21 days, where patients who died were assigned -1 day. The primary analysis was a bayesian cumulative logistic model that included all patients enrolled with severe COVID-19, adjusting for age, sex, site, region, time, assignment to interventions within other domains, and domain and intervention eligibility. Superiority was defined as the posterior probability of an odds ratio greater than 1 (threshold for trial conclusion of superiority >99%). Results: After excluding 19 participants who withdrew consent, there were 384 patients (mean age, 60 years; 29% female) randomized to the fixed-dose (n = 137), shock-dependent (n = 146), and no (n = 101) hydrocortisone groups; 379 (99%) completed the study and were included in the analysis. The mean age for the 3 groups ranged between 59.5 and 60.4 years; most patients were male (range, 70.6%-71.5%); mean body mass index ranged between 29.7 and 30.9; and patients receiving mechanical ventilation ranged between 50.0% and 63.5%. For the fixed-dose, shock-dependent, and no hydrocortisone groups, respectively, the median organ support-free days were 0 (IQR, -1 to 15), 0 (IQR, -1 to 13), and 0 (-1 to 11) days (composed of 30%, 26%, and 33% mortality rates and 11.5, 9.5, and 6 median organ support-free days among survivors). The median adjusted odds ratio and bayesian probability of superiority were 1.43 (95% credible interval, 0.91-2.27) and 93% for fixed-dose hydrocortisone, respectively, and were 1.22 (95% credible interval, 0.76-1.94) and 80% for shock-dependent hydrocortisone compared with no hydrocortisone. Serious adverse events were reported in 4 (3%), 5 (3%), and 1 (1%) patients in the fixed-dose, shock-dependent, and no hydrocortisone groups, respectively. Conclusions and Relevance: Among patients with severe COVID-19, treatment with a 7-day fixed-dose course of hydrocortisone or shock-dependent dosing of hydrocortisone, compared with no hydrocortisone, resulted in 93% and 80% probabilities of superiority with regard to the odds of improvement in organ support-free days within 21 days. However, the trial was stopped early and no treatment strategy met prespecified criteria for statistical superiority, precluding definitive conclusions. Trial Registration: ClinicalTrials.gov Identifier: NCT02735707

New approaches for assessing site formation of submerged lithic scatters

This study investigates the hydrodynamic processes that affect site formation of a submerged lithic scatter located at Cape Bruguieres Channel (CBC) in the NW of Western Australia through a combination of in situ, experimental and modelling approaches. A pressure sensor and current meter were deployed on the site and recorded hydrodynamic data over a three month period with maximum current speeds of 0.55 m/s recorded during spring tides. To test the potential for artefacts to be transported away from their original depositional context, a representative selection of submerged archaeological artefacts collected from the site and natural stone were subjected to a range of current speeds within a controlled flume environment. This demonstrated that all artefacts and natural stone remained stable within the flume even when experiencing current speeds approaching the maximum current velocities recorded at the site, regardless of lithic orientation relative to the current direction. In addition it was seen that mass alone did not control the hydrodynamic stability of the lithics tested, rather the hydrodynamic properties of a lithic are highly dependent on both its shape and orientation relative to current flow direction. This is a significant finding and suggests that the smaller the mass of a lithic does not necessarily correspond with preferential transport by currents caused by tides or waves. To test the potential for lithics to be transported during extreme cyclone events, a fine 30 m resolution bathymetric grid was constructed for CBC and current speeds modelled for Tropical Cyclone (TC) Damien which crossed the archipelago as a Category 3 (severe tropical cyclone) in February 2020. Modelled currents during this event reached a maximum velocity of 0.8 m/s and would be capable of transporting the smallest mass lithic but only if it was orientated in its most hydrodynamically unstable position relative to the current direction. These results suggest the Cape Bruguieres lithic scatter site has effectively remained in situ following sea level inundation at approximately 7,000 years BP

Knowing Is Not Doing: A Qualitative Study of Parental Views on Family Beverage Choice

Objective: Sugary drink consumption is associated with adverse health outcomes in children, highlighting the need for scalable family interventions that address barriers to water consumption. To inform development of a scalable, health-care-system-based intervention targeting family beverage choice, a formative qualitative study was conducted using semi-structured interviews with parents whose children were identified as over-consuming sugar-sweetened beverages (SSB) and/or fruit juice (FJ). The first goal of these interviews was to understand, in a diverse real-world patient population, what parents viewed as the primary drivers of their family’s beverage choices, and explore how these drivers might need to be addressed in order to make changes to beverage consumption. A second goal was to explore parental preferences for planned intervention components. An exploratory goal of the interviews was to examine whether knowledge, attitudes, and beliefs around family beverage choice differed across racial and ethnic groups in this sample. Design: Semi-structured phone interviews were conducted and interviews audio-recorded and transcribed. Participants: 39 parents/caregivers of children ages 1–8 who over-consumed sugary drinks as determined by screenings at pediatric visits. Phenomenon of Interest: Parents were interviewed about family beverage choices and preferences to inform development of a multi-component intervention. Analysis: Thematic analysis was performed, including comparison of themes across racial/ethnic groups. Results: Parents expressed that sugary drinks were unhealthy and water was a better alternative. Most were familiar with the health consequences of excess sugar consumption. They identified many reasons why sugary drinks are chosen over water despite this knowledge. One common reason was concern about tap water safety. Few differences were noted across racial and ethnic groups in our sample. Parents were enthusiastic about a technology-based intervention to be delivered through their child’s doctor’s office. Conclusions and Implications: Knowledge is not enough to change behavior. Beverage interventions need to be easy to access, make water more appealing, and elevate beverage choice above the “white noise” of everyday life. Delivering an intervention in a clinical setting could provide an extra level of care, while technology would reduce the amount of live contact and decrease the burden for clinicians and parents

The integrated cultural landscape of North Gidley Island : Coastal, intertidal and nearshore archaeology in Murujuga (Dampier Archipelago), Western Australia

Recent studies conducted in Murujuga Sea Country have confirmed that Indigenous Australian archaeology does not end at the modern shore. Since the earliest peopling of the Australian continent, sea levels have fluctuated, dropping as much as 130 m below modern mean sea-level during the Last Glacial Maximum (LGM). During this period, the continent (including Australia and New Guinea) represented a landmass one-third larger than present day Australia. As sea levels rose following the LGM, this extensive cultural landscape was inundated. The recent reporting of archaeological remains in a submerged context at Murujuga has enabled an integrated analysis of the archaeological landscape, based on direct evidence from archaeological sites that were originally formed on dry land, but are now located in intertidal and submerged environments. This study applies a landscape analysis centred on the submerged Cape Bruguieres channel site, and the Gidley Islands, where submerged, intertidal and coastal archaeology has been recorded. Aerial, pedestrian, and intertidal archaeological surveys were conducted to investigate the onshore and offshore landscape, providing new evidence with which to place the stone artefacts in the Cape Bruguieres channel into a wider context. Rock art engravings, grinding patches, quarries and upstanding stones – some of which are in the intertidal zone – point to the use of a landscape that is now submerged and to the possibility of discovering new underwater sites. By integrating evidence from subtidal and intertidal contexts with the onshore record, we explore the cultural landscape above and below the ‘waterline’ as a continuum

A multi-scalar approach to marine survey and underwater archaeological site prospection in Murujuga, Western Australia

During the past 20,000 years approximately one-quarter of the continental landmass of Australia was inundated by postglacial sea-level rise, submerging archaeological evidence for use of these landscapes. Underwater archaeological sites can offer substantial insights into past lifeways and adaptations to rapidly changing environments, however the vast scale of inundation presents a range of challenges in discovering such sites. Here we present a suite of methods as a model methodology for locating sites in submerged landscapes. Priority areas for survey were based on palaeoenvironmental contexts determined from the onshore archaeological record. Remote sensing was used to identify seabed composition and indicators of palaeolandscapes where high potential for human occupation and site preservation could be identified in Murujuga (or the Dampier Archipelago), northwestern Australia. Target locations were surveyed by scientific divers to test for the presence of archaeological material. Application of this methodology resulted in the discovery of the first two confirmed sub-tidal ancient Aboriginal archaeological sites on Australia's continental shelf. Survey methods are discussed for their combined value to identify different classes of landscapes and archaeological features to support future underwater site prospection

Quantification of receptor tyrosine kinase transactivation through direct dimerization and surface density measurements in single cells

Cell signaling involves dynamic changes in protein oligomerization leading to the formation of different signaling complexes and modulation of activity. Spatial intensity distribution analysis (SpIDA) is an image analysis method that can directly measure oligomerization and trafficking of endogenous proteins in single cells. Here, we show the use of SpIDA to quantify dimerization/activation and surface transport of receptor protein kinases—EGF receptor and TrkB—at early stages of their transactivation by several G protein-coupled receptors (GPCRs). Transactivation occurred on the same timescale and was directly limited by GPCR activation but independent of G-protein coupling types. Early receptor protein kinase transactivation and internalization were not interdependent for all receptor pairs tested, revealing heterogeneity between groups of GPCRs. SpIDA also detected transactivation of TrkB by dopamine receptors in intact neurons. By allowing for time and space resolved quantification of protein populations with heterogeneous oligomeric states, SpIDA provides a unique approach to undertake single cell multivariate quantification of signaling processes involving changes in protein interactions, trafficking, and activity