Re-Envisioning The Dissertation Stage Of Doctoral Study: Traditional Mistakes With Non-Traditional Learners

Doctoral students discuss the shift from learning in isolation to collaborative learning during doctoral study. Pros and cons of learning in isolation and collaborative learning will be detailed with various types of collaboration being discussed

Re-Envisioning the Dissertation Stage of Doctoral Study: Traditional Mistakes with Non-Traditional Learners

Doctoral students discuss the shift from learning in isolation to collaborative learning during doctoral study. Pros and cons of learning in isolation and collaborative learning will be detailed with various types of collaboration being discussed. Citation: Holmes, B. D., Seay, A. D., & Wilson, K. N. (2009). Re-Envisioning The Dissertation Stage Of Doctoral Study: Traditional Mistakes With Non-Traditional Learners. Journal of College Teaching & Learning (TLC), 6(8). https://doi.org/10.19030/tlc.v6i8.1109https://openriver.winona.edu/educationeddfacultyworks/1014/thumbnail.jp

Cohort Learning For Graduate Students At The Dissertation Stage

Doctoral students discuss the power of collaborative cohort learning in transforming the dissertation phase of doctoral study.  Innovative components of doctoral cohort learning and dissertation preparation are detailed

Cohort Learning for Graduate Students at the Dissertation Stage

Doctoral students discuss the power of collaborative cohort learning in transforming the dissertation phase of doctoral study. Innovative components of doctoral cohort learning and dissertation preparation are detailed.https://openriver.winona.edu/educationeddfacultyworks/1015/thumbnail.jp

Kinetics and Phenotype of Vaccine-Induced CD8+ T-Cell Responses to Toxoplasma gondii

Multiple studies have established that the ability of CD8+ T cells to act as cytolytic effectors and produce gamma interferon is important in mediating resistance to the intracellular parasite Toxoplasma gondii. To better understand the generation of the antigen-specific CD8+ T-cell responses induced by T. gondii, mice were immunized with replication-deficient parasites that express the model antigen ovalbumin (OVA). Class I tetramers specific for SIINFEKL were used to track the OVA-specific endogenous CD8+ T cells. The peak CD8+ T-cell response was found at day 10 postimmunization, after which the frequency and numbers of antigen-specific cells declined. Unexpectedly, replication-deficient parasites were found to induce antigen-specific cells with faster kinetics than replicating parasites. The generation of optimal numbers of antigen-specific CD8+ effector T cells was found to require CD4+ T-cell help. At 7 days following immunization, antigen-specific cells were found to be CD62Llow, KLRG1+, and CD127low, and they maintained this phenotype for more than 70 days. Antigen-specific CD8+ effector T cells in immunized mice exhibited potent perforin-dependent OVA-specific cytolytic activity in vivo. Perforin-dependent cytolysis appeared to be the major cytolytic mechanism; however, a perforin-independent pathway that was not mediated via Fas-FasL was also detected. This study provides further insight into vaccine-induced cytotoxic T-lymphocyte responses that correlate with protective immunity to T. gondii and identifies a critical role for CD4+ T cells in the generation of protective CD8+ T-cell responses

The Vehicle, June 1959, Vol. 1 no. 3

Vol. 1, No. 3 Table of Contents FrustrationNeil Parkerpage 3 Public FigureBert Browderpage 8 To a Young LadyNeil Parkerpage 8 Eastern -- YesterdayLinda Lyonspage 9 Glosing Won\u27t ServeD.E. Fullerpage 10 D. Linkwant at the BarD. Linkwantpage 10 The Wedgewood CupBarbara Wilson Dautpage 11 The SymptomsBert Browderpage 14 On a Charge for Over-DrawingD.E. Fullerpage 14 Information, PleaseD.E. Fullerpage 14 Query from Row Two, Seat ThreeHunkelheimerpage 15 DeceptionBarbara Wilson Dautpage 15 Binge in Mind?Anon.page 15 A CommaJean Nightingalepage 16 Miss Me, Kate!A.B. Carterpage 16 My SinsJean Nightingalepage 16https://thekeep.eiu.edu/vehicle/1002/thumbnail.jp

The Vehicle, June 1959, Vol. 1 no. 3

Vol. 1, No. 3 Table of Contents FrustrationNeil Parkerpage 3 Public FigureBert Browderpage 8 To a Young LadyNeil Parkerpage 8 Eastern -- YesterdayLinda Lyonspage 9 Glosing Won\u27t ServeD.E. Fullerpage 10 D. Linkwant at the BarD. Linkwantpage 10 The Wedgewood CupBarbara Wilson Dautpage 11 The SymptomsBert Browderpage 14 On a Charge for Over-DrawingD.E. Fullerpage 14 Information, PleaseD.E. Fullerpage 14 Query from Row Two, Seat ThreeHunkelheimerpage 15 DeceptionBarbara Wilson Dautpage 15 Binge in Mind?Anon.page 15 A CommaJean Nightingalepage 16 Miss Me, Kate!A.B. Carterpage 16 My SinsJean Nightingalepage 16https://thekeep.eiu.edu/vehicle/1002/thumbnail.jp

Rift Valley fever virus structural and nonstructural proteins: recombinant protein expression and immunoreactivity against antisera from sheep

The Rift Valley fever virus (RVFV) encodes the structural proteins nucleoprotein (N), aminoterminal glycoprotein (Gn), carboxyterminal glycoprotein (Gc), and L protein, 78-kD, and the nonstructural proteins NSm and NSs. Using the baculovirus system, we expressed the full-length coding sequence of N, NSs, NSm, Gc, and the ectodomain of the coding sequence of the Gn glycoprotein derived from the virulent strain of RVFV ZH548. Western blot analysis using anti-His antibodies and monoclonal antibodies against Gn and N confirmed expression of the recombinant proteins, and in vitro biochemical analysis showed that the two glycoproteins, Gn and Gc, were expressed in glycosylated form. Immunoreactivity profiles of the recombinant proteins in western blot and in indirect enzyme-linked immunosorbent assay against a panel of antisera obtained from vaccinated or wild type (RVFV)-challenged sheep confirmed the results obtained with anti-His antibodies and demonstrated the suitability of the baculo-expressed antigens for diagnostic assays. In addition, these recombinant proteins could be valuable for the development of diagnostic methods that differentiate infected from vaccinated animals (DIVA)

Correlation functions quantify super-resolution images and estimate apparent clustering due to over-counting

We present an analytical method to quantify clustering in super-resolution localization images of static surfaces in two dimensions. The method also describes how over-counting of labeled molecules contributes to apparent self-clustering and how the effective lateral resolution of an image can be determined. This treatment applies to clustering of proteins and lipids in membranes, where there is significant interest in using super-resolution localization techniques to probe membrane heterogeneity. When images are quantified using pair correlation functions, the magnitude of apparent clustering due to over-counting will vary inversely with the surface density of labeled molecules and does not depend on the number of times an average molecule is counted. Over-counting does not yield apparent co-clustering in double label experiments when pair cross-correlation functions are measured. We apply our analytical method to quantify the distribution of the IgE receptor (Fc{\epsilon}RI) on the plasma membranes of chemically fixed RBL-2H3 mast cells from images acquired using stochastic optical reconstruction microscopy (STORM) and scanning electron microscopy (SEM). We find that apparent clustering of labeled IgE bound to Fc{\epsilon}RI detected with both methods arises from over-counting of individual complexes. Thus our results indicate that these receptors are randomly distributed within the resolution and sensitivity limits of these experiments.Comment: 22 pages, 5 figure