54 research outputs found
New Types of Protein Tyrosine Phosphatase 1B Inhibitors from Marine and Terrestrial Organisms
東北医科薬科大学平成28年
Chemical Investigation and Antimicrobial Activity of Medicinal Plant <i>Toddalia asiatica</i> Lam
Medicinal plants have become important sources of natural products, which have been used in the development of therapeutic agents. Four new coumarins (1-4) have been isolated together with five known metabolites (5-9) from the medicinal plant T. asiatica. The structures of 1-9 were assigned based on their spectroscopic data. Compounds (1-9) inhibited the growth of the Gram-negative bacteria E. coli and Gram-positive bacteria S. aureus at a concentration of 25 and 50 μg/disc. Compounds (2-4) inhibited the phytopathogenic fungus C. cucurbitarum at 50 μg/disc
The Effects of Gamma Irradiation on Growth Response of Rodent Tuber (Typhonium flagelliforme Lodd.) Mutant in In Vitro Culture
Rodent tuber (Typhonium flagelliforme Lodd.) is an Indonesian native plant that potential as anticancer. Even though the potential use of bioactive compounds from T. flagelliforme is very high, its genetic variation in Indonesia is low. Gamma irradiation can be used to increase genetic variation. The objective of this research was to obtain the first generation mutants (M1) of T. flagelliforme resulting from gamma irradiation of normal mother plantlets with different plant height and number of shoots. T. flagelliforme was irradiated at the doses of 0, 10, 20, 30, 40, and 50 Gy. The percentage of plant death was then calculated to determine the Lethal Dose (LD) 50. T. flagelliforme was then re-irradiated at LD50 dose to obtain M1 mutant. The plant height and number of shoots variation was observed after eight weeks of culture. The analysis of T. flagelliforme radiosensitivity resulted in LD50 at 25 Gy. Observation at six weeks after irradiation showed significant differences between the normal mother plant, 20 Gy mutants, and 25 Gy mutants with an average plant height of 9.57, 3.41, and 2.43 cm respectively, and the average number of shoots produced was 7.85, 6.03, and 5.00 shoots respectively. Irradiation at 20 and 25 Gy produced 49 and 37 mutant plantlets. This research showed that gamma irradiation at LD50 dose could generate plant height and number of shoots variation of M1 mutant plantlets that are different from normal mother plantlets
ANTIANGIOGENESIS AND ANTIBACTERIAL ACTIVITIES FROM AN INDONESIAN MARINE-DERIVED FUNGUS Dactylaria sp
Marine-derived fungi have been proven to be rich sources of chemically diverse natural products with a broad range of biological activities. The aim of this study was to determine the antibacterial and antiangiogenesis activities of marine derived fungi Dactylaria sp. Cultivation of the fungus Dactylaria sp (strain TID 24041021-1) was isolated from the marine invertebrate sponge. Culture of marine fungus was macerated with acetone and partitioned with ethyl acetate. The ethyl acetate extracts with 50, 100, and 200µg/mL concentrations, were assayed for their antiangiogenic activity by using chorioallantoic membrane in vivo. At the same time, ethyl acetate extracts at levels of 5, 10, 15, and 20mg/mL were assayed to pathogenic bacteria Bacillus subtilis, Escherichia coli, and Staphylococcus aureus using well diffusion method. The result of this study showed that ethyl acetate extract at concentration 50µg/mL could inhibit angiogenesis. The best antiangiogenic activity was showed at concentration of 200µg/mL ethyl acetate extract. Antibacterial activity from ethyl acetate extract inhibited the growth of B. subtilis (2.25-5mm), E. coli (0.63-3.50mm) and S. aureus (01.75mm) bacteria.
Key words: Marine sponge-derived fungi, antiangiogenesis activity, antibacterial activit
New Types of Protein Tyrosine Phosphatase 1B Inhibitors from Marine and Terrestrial Organisms
平成28年
Antimicrobial metabolites from a marine-derived fungus
A marine fungus was separated from the surface of a marine red algae. Ethyl acetate extract of the cultivated marine fungus inhibited the growth of the pathogenic bacteria E.coli and S.aureus, and the phytopathogenic fungus C. cucurbitarum. Separation of the EtOAc extract gave four compounds, which were identified as new anthraquinonederivatives 3 and 4, and the known chrysophanol (1) and rubelin A (2) by the extensive analysis of NMR data. Compounds 1-4 inhibited the growth of the gram negative bacteria E. coli and gram positive bacteria S. aureus. Compound 3 also inhibited the fungus C. cucurbitarum
ANTIMICROBIAL METABOLITES FROM A MARINE-DERIVED FUNGUS
A marine fungus was separated from the surface of a marine red algae. Ethyl acetate extract of the cultivated marine fungus inhibited the growth of the pathogenic bacteria E.coli and S.aureus, and the phytopathogenic fungus C. cucurbitarum. Separation of the EtOAc extract gave four compounds, which were identified as new anthraquinonederivatives 3 and 4, and the known chrysophanol (1) and rubelin A (2) by the extensive analysis of NMR data. Compounds 1-4 inhibited the growth of the gram negative bacteria E. coli and gram positive bacteria S. aureus. Compound 3 also inhibited the fungus C. cucurbitarum
Detection of Gamma-Irradiated Mutant of Rodent Tuber (Typhonium Flagelliforme Lodd.) in Vitro Culture by RAPD Molecular Marker
Uji Ekstrak Etanol Daun Benalu Kersen (Dendropthoe Pentrandra L.) terhadap Tikus Putih (Ratus Norvegicus) sebagai Anti Inflamasi
Benalu Kersen (Dendrophthoe pentandra) merupakan tumbuhan parasit pada pohon kersen dengan mengambil makanan dari tubuh inangnya, sehingga kandungan senyawa aktif yang dimiliki benalu kersen sama dengan tumbuhan inangnya. Tujuan penelitian ini Untuk mengetahui aktivitas daun benalu kersen (Dendrophthoe pentandra) sebagai anti inflamasi pada tikus putih (Rattus norvegicus). Penelitian ini merupakan jenis penelitian eksperimental dengan rancangan acak lengkap (RAL).Hasil penelitian ini menunjukkan Ekstrak etanol etanol daun benalu tanaman kersen memiliki aktivitas antiinflamasi pada telapak kaki tikus putih yang diinduksi formalin 1%. Kemampuan antiinflamasi akan lebih signifikan dengan dosis ekstrak etanol yang lebih tinggi lagi, hal ini dibuktikan dengan kelompok perlakuan ekstrak etanol dosis 300 mg/kgBB menunjukkan penghambatan paling baik dibandingkan dengan kelompok perlakuan dosis 75 mg/kgBB dan 150 mg/kgB
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