Geschichtlich-kritische Feldzüge durch das nordöstliche Westfalen

von W. FrickeEnthält: A. Varus und Germanicus im nordöstlichen Westfalen, B. Die sogenannten Bauernburgen am Wesergebirge und Teutoburgerwald

Changes in the fatty acid composition of brown shrimp, Crangon crangon, after boiling

Brown shrimp, Crangon crangon (L.), is the most valuable target of coastal fisheries in the southern North Sea. Annual landings exceeded 30,000 tons in the last decade, yielding up to 100 Mio Euro. The shrimp are boiled immediately after capture onboard the fishing vessel for preservation and easy peeling. After landing, the shrimp are collected by traders and exported for manual peeling. Only the muscle of the pleon is returned and sold as regional delicacy. The remains, comprising the cephalothorax, the shell of the pleon, and, in case, adhering eggs, account for up to 70% of the total body mass. This potential resource, for example as aquaculture feed, has not yet been considered for exploitation. In this respect, the fatty acid (FA) profile and the share of essential FAs are crucial quality factors. Since boiling alters the quality of shrimp, this study evaluates changes in the FA composition of shrimp muscle and remains by comparing frozen and boiled samples. Major FAs in C. crangon were the saturated palmitic acid (PA, 16:0), accounting for 16.6%–19.1% of total fatty acids (TFAs), and the long-chain polyunsaturated FAs (LC-PUFAs) eicosapentaenoic acid (EPA, 20:5(n-3), 16.1–21.6%TFA ) and docosahexaenoic acid (DHA, 22:6(n-3), 11.5–13.6%TFA ). Frozen muscle and frozen remains showed similar FA profiles. Boiling changed the FA profile. PA, EPA, and DHA decreased by up to 25%, whereas palmitoleic acid 16:1(n-7) and oleic acid 18:1(n-9) increased by 2% to 3% each. Boiled muscle and boiled remains showed similar FA profiles. Despite the loss of FAs, the boiled shrimp remains are suggested to be a suitable PUFA supplement for aquaculture feeds, deserving further investigation

The Mass of the Milky Way: Limits from a Newly Assembled Set of Halo Objects

We set new limits on the mass of the Milky Way, making use of the latest kinematic information for Galactic satellites and halo objects. In order to bind these sample objects to the Galaxy, their rest-frame velocities must be lower than their escape velocities at their estimated distances. This constraint enables us to show that the mass estimate of the Galaxy is largely affected by several high-velocity objects (Leo I, Pal 3, Draco, and a few FHB stars), not by a single object alone (such as Leo I), as has often been the case in past analyses. We also find that a gravitational potential that gives rise to a declining rotation curve is insufficient to bind many of our sample objects to the Galaxy; a possible lower limit on the mass of the Galaxy is about 2.2 x 10^12 Msolar. To be more quantitative, we adopt a Bayesian likelihood approach to reproduce the observed distribution of the current positions and motions of the sample, in a prescribed Galactic potential that yields a flat rotation curve. This method enables a search for the most likely total mass of the Galaxy, without undue influence in the final result arising from the presence or absence of Leo I, provided that both radial velocities and proper motions are used. The most likely total mass derived from this method is 2.5^+0.5_-1.0 x 10^12 Msolar (including Leo I), and 1.8^+0.4_-0.7 x 10^12 Msolar (excluding Leo I).Comment: 14 pages, including 9 figures and 3 tables, accepted for publication in Astronomy and Astrophysic

Adaptations of Pseudoxylaria towards a comb-associated lifestyle in fungus-farming termite colonies

Characterizing ancient clades of fungal symbionts is necessary for understanding the evolutionary process underlying symbiosis development. In this study, we investigated a distinct subgeneric taxon of Xylaria (Xylariaceae), named Pseudoxylaria, whose members have solely been isolated from the fungus garden of farming termites. Pseudoxylaria are inconspicuously present in active fungus gardens of termite colonies and only emerge in the form of vegetative stromata, when the fungus comb is no longer attended ("sit and wait" strategy). Insights into the genomic and metabolic consequences of their association, however, have remained sparse. Capitalizing on viable Pseudoxylaria cultures from different termite colonies, we obtained genomes of seven and transcriptomes of two Pseudoxylaria isolates. Using a whole-genome-based comparison with free-living members of the genus Xylaria, we document that the association has been accompanied by significant reductions in genome size, protein-coding gene content, and reduced functional capacities related to oxidative lignin degradation, oxidative stress responses and secondary metabolite production. Functional studies based on growth assays and fungus-fungus co-cultivations, coupled with isotope fractionation analysis, showed that Pseudoxylaria only moderately antagonizes growth of the termite food fungus Termitomyces, and instead extracts nutrients from the food fungus biomass for its own growth. We also uncovered that Pseudoxylaria is still capable of producing structurally unique metabolites, which was exemplified by the isolation of two novel metabolites, and that the natural product repertoire correlated with antimicrobial and insect antifeedant activity

Adaptations of Pseudoxylaria towards a comb-associated lifestyle in fungus-farming termite colonies

DATA AVAILABILITY: Supporting Information of this article is free of charge and contains list of accession numbers of sequences used for analysis, phylogenetic trees, cultivation studies including co-cultivation, analyses of genomic and metabolomic data, NMR and MS-data of isolated metabolites and data of insect feeding studies including statistical analyses.Characterizing ancient clades of fungal symbionts is necessary for understanding the evolutionary process underlying symbiosis development. In this study, we investigated a distinct subgeneric taxon of Xylaria (Xylariaceae), named Pseudoxylaria, whose members have solely been isolated from the fungus garden of farming termites. Pseudoxylaria are inconspicuously present in active fungus gardens of termite colonies and only emerge in the form of vegetative stromata, when the fungus comb is no longer attended (“sit and wait” strategy). Insights into the genomic and metabolic consequences of their association, however, have remained sparse. Capitalizing on viable Pseudoxylaria cultures from different termite colonies, we obtained genomes of seven and transcriptomes of two Pseudoxylaria isolates. Using a whole-genome-based comparison with free-living members of the genus Xylaria, we document that the association has been accompanied by significant reductions in genome size, protein-coding gene content, and reduced functional capacities related to oxidative lignin degradation, oxidative stress responses and secondary metabolite production. Functional studies based on growth assays and fungus-fungus co-cultivations, coupled with isotope fractionation analysis, showed that Pseudoxylaria only moderately antagonizes growth of the termite food fungus Termitomyces, and instead extracts nutrients from the food fungus biomass for its own growth. We also uncovered that Pseudoxylaria is still capable of producing structurally unique metabolites, which was exemplified by the isolation of two novel metabolites, and that the natural product repertoire correlated with antimicrobial and insect antifeedant activity.The German Research Foundation (DFG, Deutsche Forschungsgemeinschaft), the Germany´s Excellence Strategy, the European Research Council and The Danish Council for Independent Research. Open Access funding enabled and organized by Projekt DEAL.https://www.nature.com/ismejBiochemistryGeneticsMicrobiology and Plant Patholog

Mammalian BTBD12 (SLX4) Protects against Genomic Instability during Mammalian Spermatogenesis

The mammalian ortholog of yeast Slx4, BTBD12, is an ATM substrate that functions as a scaffold for various DNA repair activities. Mutations of human BTBD12 have been reported in a new sub-type of Fanconi anemia patients. Recent studies have implicated the fly and worm orthologs, MUS312 and HIM-18, in the regulation of meiotic crossovers arising from double-strand break (DSB) initiating events and also in genome stability prior to meiosis. Using a Btbd12 mutant mouse, we analyzed the role of BTBD12 in mammalian gametogenesis. BTBD12 localizes to pre-meiotic spermatogonia and to meiotic spermatocytes in wildtype males. Btbd12 mutant mice have less than 15% normal spermatozoa and are subfertile. Loss of BTBD12 during embryogenesis results in impaired primordial germ cell proliferation and increased apoptosis, which reduces the spermatogonial pool in the early postnatal testis. During prophase I, DSBs initiate normally in Btbd12 mutant animals. However, DSB repair is delayed or impeded, resulting in persistent γH2AX and RAD51, and the choice of repair pathway may be altered, resulting in elevated MLH1/MLH3 focus numbers at pachynema. The result is an increase in apoptosis through prophase I and beyond. Unlike yeast Slx4, therefore, BTBD12 appears to function in meiotic prophase I, possibly during the recombination events that lead to the production of crossovers. In line with its expected regulation by ATM kinase, BTBD12 protein is reduced in the testis of Atm−/− males, and Btbd12 mutant mice exhibit increased genomic instability in the form of elevated blood cell micronucleus formation similar to that seen in Atm−/− males. Taken together, these data indicate that BTBD12 functions throughout gametogenesis to maintain genome stability, possibly by co-ordinating repair processes and/or by linking DNA repair events to the cell cycle via ATM

Der Teutoburgerwald, das Wesergebirge und die Stadt Bielefeld : ein Führer durch das nordöstliche Westfalen, umfassend die Gebiete zwischen Bielefeld, Osnabrück, Höxter, und Minden, Steinhuder-Meer und Hannover

von W. Frick

Der Untergang der Stedinger : eine geschichtliche Erzählung aus der Vorzeit von Köln, Hamm und Bremen

In Fraktu