Coumarins from Peucedanum luxurians

Ten coumarins were isolated from Peucedanum luxurians. The rare coumarins stenocarpin, stenocarpin isobutyrate, officinalin, officinalin isobutyrate, 8-methoxypeucedanin and the known xanthotoxin, isoimperatorin, bergaptene, peucedanin and cnidilin were isolated. All isolated compound were identified by means of spectral data. © 2007 Elsevier B.V. All rights reserved

Antimicrobial activity of petroleum ether and methanolic extracts from fruits of Seseli devenyense Simonk. and the herb of Peucedanum luxurians Tamam

Plants of the Apiaceae family usually contain coumarins. These are used worldwide in traditional medicine, as well as in modern therapeutics. The aim of our study was to determine the antimicrobial activity of four extracts (methanolic and petroleum ether extracts) obtained from two Apiaceae species: Seseli devenyense (fruits) and Peucedanum luxurians (herb). The activity of the investigated extracts was tested against 7 strains of Gram+ bacteria, and 7 strains of Gram-, as well as three of yeast. The results of this show that the best activity of such extracts (specifically, by way of petroleum ether) was seen as being against Staphylococcus aureus strains. © 2015 Medical University of Lublin

Volatiles from selected apiaceae species cultivated in poland-antimicrobial activities

As part of our ongoing research on phytoconstituents that can act as promising antimicrobial agents, the essential oils of nine selected Apiaceae plants, cultivated in Poland, were studied. The volatiles of the aerial parts with fruits (herba cum fructi) of Silaum silaus, Seseli devenyense, Seseli libanotis, Ferula assa-foetida, Glehnia littoralis and Heracleum dulce, in addition to the fruits (fructi) of Torilis japonica and Orlaya grandiflora as well as of the aerial parts (herba) of Peucedanum luxurians were investigated through Gas Chromatography-Mass Spectrometry to identify more than 60 different metabolites. The essential oils from S. devenyense, H. dulce, T. japonica and P. luxurians are reported for the first time. All examined species were also assayed for their antimicrobial activities against several human pathogenic Gram-positive and -negative bacteria and fungi. The species H. dulce, S. devenyense and S. libanotis exerted the strongest antimicrobial activity, mostly against Gram-positive bacteria strains (MIC values 0.90-1.20 mg/mL). To the best of our knowledge, this is the first attempt to determine the antimicrobial activity of the above Apiaceae species. © 2021 by the authors. Licensee MDPI, Basel, Switzerland

Coumarins from angelica lucida L. - Antibacterial activities

The first phytochemical investigation of the fruits of Angelica lucida has led to the isolation and characterization of five known coumarins (imperatorin, isoimperatorin, heraclenol, oxypeucedanin hydrate and heraclenin). All isolated compounds were identified by means of spectral and literature data. The extracts and the isolated constituents from A. lucida have been also evaluated for their antimicrobial activity against six Gram positive and negative bacteria, two oral pathogens and three human pathogenic fungi, exhibiting an interesting antimicrobial profile. © 2009

Coumarins from the fruits of Seseli devenyense

Eight new coumarins were isolated from the fruits of Seseli devenyense Simonkai. Their structures were established from NMR and mass data and their absolute configurations from chemical degradation correlation reactions. The new structures are the decanoic and dodecanoic esters of (+)-lomatin (3, 4), the decanoates of (+)-cis-khellactone at positions 4′ (5) and 3′ (6) as well as the 2′S epimer of 8-(2,3-dihydroxy-3-methylbutyl)-7- hydroxychromen-2-one (7) named devenyol, its two O-monoglucosides at positions 3′ and 7 named devenyosides A (8) and B (9), and the corresponding 3′- and 7-O-diglucoside named devenyoside C (10). This plant is an interesting example of stereochemical diversity based on biodiversity given that other members of the Apiaceae family produce exclusively the 2′R epimers of compounds 7-9. © 2005 American Chemical Society and American Society of Pharmacognosy

Isolation and antimicrobial activity of coumarin derivatives from fruits of peucedanum luxurians tamamsch

As a continuation of searching for phytoconstituents that act as promising agents for antimicrobial therapy, rare coumarins were isolated from fruits of Peucedanum luxurians and tested. In a first step, the content of major compounds in the aerial parts and fruits of P. luxurians were compared. The results clearly showed that the fruits with dichloromethane as a solvent yielded, in most cases, higher concentrations of almost all the analyzed coumarins than the aerial parts, with peucedanin detected as the most abundant compound with a concentration of 4563.94 ± 3.35 mg/ 100 g. Under this perspective, the dichloromethane extract from the fruits of P. luxurians was further submitted to high performance countercurrent chromatography with a mixture of n-hexane, ethyl acetate, methanol, and water 6:5:6:5 (v/v). Combination of HPCCC and prep-HPLC yielded 6,7-dihydroxybergamottin (1), officinalin (2), stenocarpin isobutyrate (3), officinalin isobutyrate (4), 8-methoxypeucedanin (5), and peucedanin (6). Isolated compounds were tested against several Gram-positive and Gram-negative bacteria strains. 6,7-Dihydroxybergamottin, peucedanin, and officinalin isobutyrate appeared to be the most active against all tested bacteria strains with minimum inhibitory concentration (MIC) values between 1.20 and 4.80 mg/mL. To the best of our knowledge, this is the first report about countercurrent isolation of mentioned coumarins, as well as the first information about their antimicrobial activity. © 2018 by the authors

Rare coumarins induce apoptosis, G1 cell block and reduce RNA content in HL60 cells

The rare coumarins stenocarpin, stenocarpin isobutyrate, oficinalin, oficinalin isobutyrate, 8-methoxypeucedanin and the known xanthotoxin, isoimperatorin, bergapten, peucedanin and 8-methoxyisoimperatorin were isolated from Peucedanum luxurians Tamamsch. (Apiaceae) and identified by means of spectral data (1D and 2D NMR). Their immunomodulating activity was evaluated by flow cytometry and their influence on HL60 cells as well as on PHA-stimulated PBLs was tested. All tested coumarins induce apoptosis (maximal in the 48 h culture) and decrease cell proliferation in a time- and dose-dependent manner, especially in HL60 cells. They also induce partial G1 block, but only in HL60 cells (at 100 μM concentrations). Dose-dependent reduction of RNA content was also found in G1 cells treated by the coumarins. All of the tested coumarins also possessed immunomodulatory activities. Bergapten and xanthotoxin were found to be the best candidates for further evaluation as anti-cancer drugs. © 2017 Jarosław Widelski et al

Nanoapatites Doped and Co-Doped with Noble Metal Ions as Modern Antibiofilm Materials for Biomedical Applications against Drug-Resistant Clinical Strains of Enterococcus faecalis VRE and Staphylococcus aureus MRSA

The main aim of our research was to investigate antiadhesive and antibiofilm properties of nanocrystalline apatites doped and co-doped with noble metal ions (Ag+, Au+, and Pd2+) against selected drug-resistant strains of Enterococcus faecalis and Staphylococcus aureus. The materials with the structure of apatite (hydroxyapatite, nHAp; hydroxy-chlor-apatites, OH-Cl-Ap) containing 1 mol% and 2 mol% of dopants and co-dopants were successfully obtained by the wet chemistry method. The majority of them contained an additional phase of metallic nanoparticles, in particular, AuNPs and PdNPs, which was confirmed by the XRPD, FTIR, UV–Vis, and SEM–EDS techniques. Extensive microbiological tests of the nanoapatites were carried out determining their MIC, MBC value, and FICI. The antiadhesive and antibiofilm properties of the tested nanoapatites were determined in detail with the use of fluorescence microscopy and computer image analysis. The results showed that almost all tested nanoapatites strongly inhibit adhesion and biofilm production of the tested bacterial strains. Biomaterials have not shown any significant cytotoxic effect on fibroblasts and even increased their survival when co-incubated with bacterial biofilms. Performed analyses confirmed that the nanoapatites doped and co-doped with noble metal ions are safe and excellent antiadhesive and antibiofilm biomaterials with potential use in the future in medical sectors

Characterization and biological evaluation of propolis from Poland

In this study, we assessed the therapeutic potential of propolis from Poland and performed chemical analysis by GC-MS, as well as determined its botanical origin. Chemical constituents typical for bud exudates of Populus nigra (section Aigeiros) were determined, however, glycerol esters of phenolic acids, as well as unusually high amounts of p-coumaric and ferulic acid and their benzyl esters, were also detected. These constituents are characteristic for buds of Populus tremula (section Leuce). We also evaluated the antiproliferative effect of propolis extracts against nine human cancer cell lines. Additionally, promising antibacterial activity of the dichloromethane extract (Minimal Inhibitory Concentration MIC values of 0.95-1.24 mg/mL), as well as a moderate antifungal activity (MIC values of 1.25-1.40 mg/mL), was noticed. Propolis from Poland appeared as a rich source of antibacterial and antiproliferative compounds and this confirmed that it is a valuable natural product with the potential to improve human health. © 2017 by the authors