An optical phase-locking with large and tunable frequency difference based on vertical-cavity surface-emitting laser

We present a novel technique to phase-lock two lasers with controllable frequency difference. In our setup, one sideband of a current modulated Vertical-Cavity Surface-Emitting Laser (VCSEL) is phase locked to the master laser by injection seeding, while another sideband of the VCSEL is used to phase lock the slave laser. The slave laser is therefore locked in phase with the master laser, with a frequency difference tunable up to about 35 GHz. The sideband suppression rate of the slave laser is more than 30dB at 30 uW seed power. The heterodyne spectrum between master and slave has a linewidth of less than 1 Hz. A coherent population trapping resonance of rubidium is achieved using such beams.Comment: 4 pages, 4 Encapsulated PostScript figure

Inside Audit Firms

We develop and test hypotheses about compensation policy and auditor retention in accounting firms. Our analyses use de-identified employment and compensation data to investigate the entire pay distribution within accounting firms. Accounting firms all have low retention rates but exhibit differing pay structures. Big 4 firms give similar raises within each cohort, while non-Big 4 give substantial raises to a few top performers. Auditors often "move up" to Big 4 firms, but relatively few move the other way. Audit fees are consistently related to compensation structure. Overall, our results suggest that compensation policies in professional accounting firms affect auditor behavior

Identification of early gene expression changes in primary cultured neurons treated with topoisomerase I poisons.

Topoisomerase 1 (TOP1) poisons like camptothecin (CPT) are currently used in cancer chemotherapy but these compounds can have damaging, off-target effects on neurons leading to cognitive, sensory and motor deficits. To understand the molecular basis for the enhanced sensitivity of neurons to CPT, we examined the effects of compounds that inhibit TOP1-CPT, actinomycin D (ActD) and β-lapachone (β-Lap)-on primary cultured rat motor (MN) and cortical (CN) neurons as well as fibroblasts. Neuronal cells expressed higher levels of Top1 mRNA than fibroblasts but transcript levels are reduced in all cell types after treatment with CPT. Microarray analysis was performed to identify differentially regulated transcripts in MNs in response to a brief exposure to CPT. Pathway analysis of the differentially expressed transcripts revealed activation of ERK and JNK signaling cascades in CPT-treated MNs. Immediate-early genes like Fos, Egr-1 and Gadd45b were upregulated in CPT-treated MNs. Fos mRNA levels were elevated in all cell types treated with CPT; Egr-1, Gadd45b and Dyrk3 transcript levels, however, increased in CPT-treated MNs and CNs but decreased in CPT-treated fibroblasts. These transcripts may represent new targets for the development of therapeutic agents that mitigate the off-target effects of chemotherapy on the nervous system

Frequency Coupling Admittance Modeling of Quasi-PR Controlled Inverter and Its Stability Comparative Analysis under the Weak Grid

This paper intends to comparatively study the stabilities of grid-connected inverters with three closely related controllers: quasi-proportional resonance (quasi-PR), proportional integral (PI), and proportional resonance (PR) under the weak grid. Firstly, considering the influence of frequency coupling characteristic, a frequency coupling admittance model of quasi-PR controlled inverter is established. Then, the admittance characteristics of the quasi-PR, PI and PR controlled inverters are compared. Admittance characteristics of the PI and PR controlled inverters are similar while the quasi-PR controlled inverter is quite different: the amplitude of the quasi-PR controlled inverter is larger than that of the PI controlled inverter and the phase difference between the two inverters is obvious in the mid-high frequency areas, which are mainly caused by the resonance bandwidth of the quasi-PR controller. Furthermore, the stabilities of the quasi-PR, PI and PR controlled inverters are analyzed. The stabilities of the PI and PR controlled inverters are similar but the quasi-PR controlled inverter is more sensitive to weak grid and high inverter output power. To achieve the same system stability, the voltage outer-loop bandwidth of the quasi-PR controlled inverter should be designed narrower than that of the PI and PR controlled inverters. Finally, experiments verify the correctness of the analyses

Calibrating the absorption imaging of cold atoms under high magnetic fields

We develop a theoretical model for calibrating the absorption imaging of cold atoms under high magnetic fields. Comparing to zero or low magnetic fields, the efficiency of the absorption imaging becomes lower while it requires an additional correction factor to obtain the absolute atom number under the Beer-Lambert law. Our model is based on the rate equations and can account many experimental imperfections such as Zeeman level crossing, failures of hyperfine structures, off-resonant couplings, and low repumping efficiency, etc. Based on this method, we can precisely calculate the correction factor for atom number measurement without any empirical or fitting parameters. Meanwhile, we use a cold-atom apparatus of rubidium-85 to experimentally verify our model. Besides these, we find our work can also serve as a benchmark to measure the polarization impurity of a circular-polarized laser beam with high sensitivities. We believe this work will bring convenience for most of cold-atom experiments using absorption imaging.Comment: 9 pages, 5 figure

Nrdp1 Increases Ischemia Induced Primary Rat Cerebral Cortical Neurons and Pheochromocytoma Cells Apoptosis Via Downregulation of HIF-1α Protein

Neuregulin receptor degradation protein-1 (Nrdp1) is an E3 ubiquitin ligase that targets proteins for degradation and regulates cell growth, apoptosis and oxidative stress in various cell types. We have previously shown that Nrdp1 is implicated in ischemic cardiomyocyte death. In this study, we investigated the change of Nrdp1 expression in ischemic neurons and its role in ischemic neuronal injury. Primary rat cerebral cortical neurons and pheochromocytoma (PC12) cells were infected with adenoviral constructs expressing Nrdp1 gene or its siRNA before exposing to oxygen-glucose deprivation (OGD) treatment. Our data showed that Nrdp1 was upregulated in ischemic brain tissue 3 h after middle cerebral artery occlusion (MCAO) and in OGD-treated neurons. Of note, Nrdp1 overexpression by Ad-Nrdp1 enhanced OGD-induced neuron apoptosis, while knockdown of Nrdp1 with siRNA attenuated this effect, implicating a role of Nrdp1 in ischemic neuron injury. Moreover, Nrdp1 upregulation is accompanied by increased protein ubiquitylation and decreased protein levels of ubiquitin-specific protease 8 (USP8) in OGD-treated neurons, which led to a suppressed interaction between USP8 and HIF-1α and subsequently a reduction in HIF-1α protein accumulation in neurons under OGD conditions. In conclusion, our data support an important role of Nrdp1 upregulation in ischemic neuronal death, and suppressing the interaction between USP8 and HIF-1α and consequently the hypoxic adaptive response of neurons may account for this detrimental effect

Transcriptome profiling of spinal muscular atrophy motor neurons derived from mouse embryonic stem cells.

Proximal spinal muscular atrophy (SMA) is an early onset, autosomal recessive motor neuron disease caused by loss of or mutation in SMN1 (survival motor neuron 1). Despite understanding the genetic basis underlying this disease, it is still not known why motor neurons (MNs) are selectively affected by the loss of the ubiquitously expressed SMN protein. Using a mouse embryonic stem cell (mESC) model for severe SMA, the RNA transcript profiles (transcriptomes) between control and severe SMA (SMN2+/+;mSmn-/-) mESC-derived MNs were compared in this study using massively parallel RNA sequencing (RNA-Seq). The MN differentiation efficiencies between control and severe SMA mESCs were similar. RNA-Seq analysis identified 3,094 upregulated and 6,964 downregulated transcripts in SMA mESC-derived MNs when compared against control cells. Pathway and network analysis of the differentially expressed RNA transcripts showed that pluripotency and cell proliferation transcripts were significantly increased in SMA MNs while transcripts related to neuronal development and activity were reduced. The differential expression of selected transcripts such as Crabp1, Crabp2 and Nkx2.2 was validated in a second mESC model for SMA as well as in the spinal cords of low copy SMN2 severe SMA mice. Furthermore, the levels of these selected transcripts were restored in high copy SMN2 rescue mouse spinal cords when compared against low copy SMN2 severe SMA mice. These findings suggest that SMN deficiency affects processes critical for normal development and maintenance of MNs