8 research outputs found
Low Dosage of Histone H4 Leads to Growth Defects and Morphological Changes in Candida albicans
- Author
- A Adams
- A Coste
- A Forche
- A Forche
- A Sellam
- A Sellam
- A Selmecki
- A Selmecki
- AB Vojtek
- AG Castillo
- AJ Klar
- AM Gillum
- Anna M. Selmecki
- ARD Ganley
- B Dorigo
- B Li
- B Slutsky
- B Wickes
- BB Magee
- C Ketel
- CD Clark-Adams
- CH Chang
- CK Lee
- CS Hoffman
- D Diogo
- D Hnisz
- D Meeks-Wagner
- D Norris
- D Pruss
- DA Davis
- Dana A. Davis
- DE Libuda
- DR Soll
- DR Soll
- DR Soll
- E Rustchenko
- EM Hyland
- EP Rustchenko
- EP Rustchenko
- EP Rustchenko-Bulgac
- ES Bensen
- F Prado
- G Janbon
- GD Moore
- GS Stein
- J Ausio
- J Choe
- J Svaren
- JJ Wyrick
- JL Workman
- JR Warner
- JRE Wells
- Judith Berman
- K Ahmad
- K Ahmad
- K Asakura
- K Sanyal
- K Tsui
- L Moran
- L Zhang
- LH Wong
- LK Durrin
- Lucia F. Zacchi
- M Arbour
- M Han
- M Han
- M Legrand
- MA Osley
- MA Pfaller
- MA Pfaller
- MA Uhl
- Michael C. Lorenz
- MJ Saunders
- MM Smith
- MM Smith
- MM Smith
- PT Magee
- RA Calderone
- RB Wilson
- RB Wilson
- RC Barton
- S Fretzin
- S Garcia-Sanchez
- S Iwaguchi
- S Matsumoto
- S Venditti
- SB Hake
- SB Raman
- SD Taverna
- SE Hanlon
- T Jenuwein
- T Jones
- T Srikantha
- T Suzuki
- TH Thatcher
- TR Hughes
- UJ Kim
- V Perepnikhatka
- W Wu
- W-C Au
- WL Smith
- X Chen
- X Mao
- Y Lu
- Y Takayama
- Publication venue
- Public Library of Science
- Publication date
- 01/01/2010
- Field of study
Chromatin function depends on adequate histone stoichiometry. Alterations in histone dosage affect transcription and chromosome segregation, leading to growth defects and aneuploidies. In the fungal pathogen Candida albicans, aneuploidy formation is associated with antifungal resistance and pathogenesis. Histone modifying enzymes and chromatin remodeling proteins are also required for pathogenesis. However, little is known about the mechanisms that generate aneuploidies or about the epigenetic mechanisms that shape the response of C. albicans to the host environment. Here, we determined the impact of histone H4 deficit in the growth and colony morphology of C. albicans. We found that C. albicans requires at least two of the four alleles that code for histone H4 (HHF1 and HHF22) to grow normally. Strains with only one histone H4 allele show a severe growth defect and unstable colony morphology, and produce faster-growing, morphologically stable suppressors. Segmental or whole chromosomal trisomies that increased wild-type histone H4 copy number were the preferred mechanism of suppression. This is the first study of a core nucleosomal histone in C. albicans, and constitutes the prelude to future, more detailed research on the function of histone H4 in this important fungal pathogen
The Search for Receptor-Interactive Regions in Growth-Hormone - Historical Perspectives and Novel Strategies
- Publication venue
- 'Wiley'
- Publication date
- 01/04/1994
- Field of study
Evidence for Involvement of the Carboxy-Terminus of Helix-1 of Growth-Hormone in Receptor-Binding - Use of Charge Reversal Mutagenesis to Account for Calcium-Dependence of Binding and for Design of Higher Affinity Analogs
- Publication venue
- 'American Chemical Society (ACS)'
- Publication date
- 01/10/1994
- Field of study
In this study we have demonstrated that the C-terminus of helix 1 of porcine GH (pGH) is a receptor-interactive region, thus extending the current binding site model of GH. This was achieved by introducing charge reversal mutations into this region of pGH, which influenced receptor affinity and Ca2+ dependence of binding. The first mutant (R34E pGH, conversion of Arg 34 to Glu) introduced a putative Ca2+ binding site which is present in human GH (hGH) [Barnard et al. (1989) J. Theor. Biol. 140, 355-367] and sits opposite E220 of receptor subunit 1. This mutant exhibited increased Ca2+ dependence of receptor binding but even at optimal Ca2+ did not display higher than wild-type affinity. Introduction of a second Ca2+ binding site adjacent to the first by a second charge reversal (K30E R34E pGH) further increased Ca2+ dependence of binding and also increased affinity for the rabbit GH receptor (2.4 +/- 0.4)-fold relative to wild-type pGH at optimal Ca2+ Equilibrium dialysis and Scatchard analysis of binding of Ca-45(2+) to PGH and K30E R34E pGH revealed two Ca2+ binding sites on wild-type pGH and an additional two Ca2+ binding sites on the K30E R34E pGH mutant (K-d 0.5-0.8 mM), as predicted. A third partial charge reversal mutant in the fourth helix (H170D) also led to enhanced Ca2+ dependence of binding, supporting our proposal that E34 and D170 are responsible for the Ca2+ dependence of hGH binding to the rabbit GH receptor. Examination of the crystal structure shows that E34 and D170 are in close proximity and would interact repulsively with a cluster of acidic residues on the receptor consisting of E126, E127, and E220 unless neutralized by Ca2+ or an introduced basic residue. Accordingly, charge reversal at the adjacent PGH residue E33 (E33K pGH) led to a Ca2+ independent (3.0 +/- 0.4)-fold increase in affinity of binding. As well as extending the binding site model of GH, these studies provide a mechanistic explanation for the unique Ca2+ dependence of hGH binding to the rabbit GH receptor. They also indicate that charge reversal can be used to design higher affinity GH analogues and could assist in the mapping of interactive regions in ligand-receptor complexes generally
Avoidance behavior and swimming activity of fish to detect pH changes
- Author
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- Field of study
Histone H3 transcript stability in alfalfa
- Author
- A Sachs
- E Lam
- E Stimac
- E Stimac
- FF Smith
- G Hadlaczky
- HX Xu
- J Chojecki
- J Conia
- JH Waterborg
- JH Waterborg
- JRE Wells
- K Mikami
- LL Baumbach
- M Delseny
- M Lepetit
- M Pfosser
- MA Osley
- MA Osley
- MA Osley
- ME Chaboute
- ME Harris
- ML Sullivan
- N Chaubet
- N Chaubet
- N Heintz
- NB Pandey
- P Brignon
- PA Watson
- PJ Green
- PJ Mosca
- R Terada
- RA Graves
- RA Graves
- RS Wu
- S-C Wu
- S-C Wu
- SW Peltz
- T Kapros
- T Kapros
- T Kapros
- T Kapros
- TC Newman
- TD Morris
- TG Sjakste
- WM Bonner
- Z Magyar
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- Field of study
Historical Extinctions in the Sea
- Author
- Abbott RT
- Baillie J
- Begon M
- Bigelow HB
- Butman CA
- Campbell RR
- Carlton JT
- Clark KB
- Clark KB
- Collar NJ
- Crane J
- Darlington PJ
- Day D
- Edmondson CH
- Ehrlich PR
- Eldredge N
- Fuller E
- Gardner JPA
- Gibson-Smith J
- Greenway JC
- Groombridge B
- Harger JRE
- Hayman P
- Hornaday WT
- Johnson ME
- Keep J
- Knudston PM
- Laws RM
- Leakey R
- Lindeboom HJ
- Lovejoy T
- MacArthur RH
- MacGinitie GE
- Marx W
- Mastro E
- Matthiessen P
- May M
- May RM
- Merrett NR
- Mikkelsen PM
- Myers N
- Nicol S
- Norse EA
- Norse EA
- Norse EA
- O'Clair RM
- Palmer AR
- Perry DA
- Race MS
- Raup DM
- Rice DW
- Ricketts EF
- Risk MJ
- Runnegar B
- Safina C
- Scheffer VB
- Silverberg R
- Taylor DW
- Vermeij GJ
- Waples RS
- Wells SM
- Wilkinson CR
- Wilson EO
- Publication venue
- 'Annual Reviews'
- Publication date
- Field of study
Hard clam walking: Active horizontal locomotion of adult Mercenaria mercenaria at the sediment surface and behavioral suppression after extensive sampling
- Author
- AD Ansell
- AD Ansell
- AD Ansell
- AM Knights
- AN Schwalb
- AW Stoner
- BA Minteer
- Bradley T. Furman
- Brooke S. Rodgers
- C Swennen
- CA Catton
- Christian R. H. Tettelbach
- CM Yonge
- D Huang
- D Roberts
- DB Quayle
- DK Padilla
- DL Balfour
- DL Belding
- DR Levitan
- DR Levitan
- DS Wethey
- EA Trippel
- ER Trueman
- ER Trueman
- Geerat J. Vermeij
- GM Yenni
- GV Fraenkel
- H Cyr
- H Kappes
- HW Wells
- I-Y Ahn
- IM Côté
- J Alcock
- JA Downing
- James R. Europe
- Jason Havelin
- JBC Jackson
- JC Clements
- JH Connell
- JH Zar
- JN Kraeuter
- JN Kraeuter
- JP Amyot
- JP Amyot
- JRE Harger
- JS Levinton
- KM Stokesbury
- KN Mouritsen
- L Seuront
- LA Wilkens
- LTS Cândido
- M Liermann
- M Lloyd
- M Morisita
- M Saarinen
- M Tallqvist
- Marissa Velasquez
- ML Botton
- MR Carriker
- PJ Clark
- PO Yund
- R Seed
- RD Barnes
- RIE Newell
- RL Dow
- RL Folk
- SH Hurlbert
- SM Stanley
- SR Fegley
- ST Tettelbach
- ST Tettelbach
- Stephen T. Tettelbach
- TC Nelson
- WH Tan
- Y Kim
- Publication venue
- 'Public Library of Science (PLoS)'
- Publication date
- Field of study
Enzyme Handbook
- Author
- 339-349
- [10]Siffert O., Emöd, I.
- [11] Fricker L.D., Snyder, S.H.
- [11] Taylor A., Volz, K.W., Lipscomb, W.N., Takemoto, L. J.
- [3] Schmidt U., Weller, D., Holder, A.
- [9]Fujiwara K., Tsuru, D.
- [hie J.N., Dure, L.S.
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- A Ducruix
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- A Stockell
- A Storer
- A Suhar
- A Suzuki
- A Suzuki
- A Szewczuk
- A Szewczuk
- A Taylor
- A Taylor
- A Thomson
- A Traverso-Cori
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- Chan. S.A.T., Jones, T.H.D., Sweeney, J.P., Toursarkissian, K.
- Chih-Min Kam Fujikawa, K.
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- Xin-Ii Lin Wong, R.N.S., Tang, J.
- Y Agrawal
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- Y Atsumi
- Y Bai
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- Y-R Chen
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- “ (Turk V., Neville, M.
- Publication venue
- 'Springer Science and Business Media LLC'
- Publication date
- 01/01/1991
- Field of study