2 research outputs found
Optical detection of single electron spin resonance in a quantum dot
We demonstrate optically detected spin resonance of a single electron
confined to a self-assembled quantum dot. The dot is rendered dark by resonant
optical pumping of the spin with a coherent laser. Contrast is restored by
applying a radio frequency (rf) magnetic field at the spin resonance. The
scheme is sensitive even to rf fields of just a few micro-T. In one case, the
spin resonance behaves exactly as a driven 3-level quantum system (a
lambda-system) with weak damping. In another, the dot exhibits remarkably
strong (67% signal recovery) and narrow (0.34 MHz) spin resonances with
fluctuating resonant positions, evidence of unusual dynamic processes of
non-Markovian character.Comment: 4 pages, 5 figure
Morphologically homogeneous red blood cells present a heterogeneous response to hormonal stimulation
Red blood cells (RBCs) are among the most intensively studied cells in natural history, elucidating numerous principles and ground-breaking knowledge in cell biology. Morphologically, RBCs are largely homogeneous, and most of the functional studies have been performed on large populations of cells, masking putative cellular variations. We studied human and mouse RBCs by live-cell video imaging, which allowed single cells to be followed over time. In particular we analysed functional responses to hormonal stimulation with lysophosphatidic acid (LPA), a signalling molecule occurring in blood plasma, with the Ca(2+) sensor Fluo-4. Additionally, we developed an approach for analysing the Ca(2+) responses of RBCs that allowed the quantitative characterization of single-cell signals. In RBCs, the LPA-induced Ca(2+) influx showed substantial diversity in both kinetics and amplitude. Also the age-classification was determined for each particular RBC and consecutively analysed. While reticulocytes lack a Ca(2+) response to LPA stimulation, old RBCs approaching clearance generated robust LPA-induced signals, which still displayed broad heterogeneity. Observing phospatidylserine exposure as an effector mechanism of intracellular Ca(2+) revealed an even increased heterogeneity of RBC responses. The functional diversity of RBCs needs to be taken into account in future studies, which will increasingly require single-cell analysis approaches. The identified heterogeneity in RBC responses is important for the basic understanding of RBC signalling and their contribution to numerous diseases, especially with respect to Ca(2+) influx and the associated pro-thrombotic activity