POTENTIALITY OF USING A. PINNATA TO BIOREMEDIATE DIFFERENT HEAVY METALS FROM POLLUTED DRAINING WATER

Azolla pinnata is a small aquatic fern which considered as a multipurpose organism. It isused in bioremediation to remove heavy metals from polluted draining waters. In view of its potentiality, Azolla pinnata were tested for their growth on different media (Yoshida, peat moss, and Soil media) to select the best medium for the growth of Azolla. Then the best medium was examined for the potentiality of Azolla to withstand a different concentration of different heavy metals. Yoshida medium was the most suitable medium, as it recorded the highest significant records in fresh and dry weight, being 25.38 and 1.69 g/ pot respectively, the doubling time was 5.43 days, NPK% being 3.87, 0.85 and 1.95% respectively and nitrogenase activity being 14.32 μmol C2H4/ g dry weight. / hr). The fresh and dry weight of Azolla exposed to Pb+2,Co+2 or Cu+2, was found to be increased by increasing the concentration of the metals from 5 to 35 ppm/liter, then they decreased when the fern exposed to 45 ppm/liter for all of them. While, doubling time decreased gradually by increasing the concentration of Pb+2 , Co+2  orCu+2 from 5 to 35 ppm/liter and started to increase by increasing the concentration of Pb+2 to 45 ppm/liter in the medium

Antibacterial and Cytotoxicity Functions of Pomegranate Peel Extracts in Fish Processing

Pomegranate peel extracts, both ethanolic (PPE) and aqueous (PPA), demonstrated a broad spectrum of antibacterial action against Gr+ve and Gr-ve bacteria, with bacterial isolate B2 (G+ve cocci) having the maximum inhibition zone (IZ) 10.5–26.5 mm at concentrations  4.2 mg mL-1 to 267 mg mL-1. PPE's minimum inhibitory concentration (MIC) varies among bacterial strains, with the lowest being 8.3 mg mL-1. Ball-milled nano-scale pomegranate peel (NSPP) with 89.09 nm particle increased IZ 3-fold and decreased MIC significantly. MTT was used to assess the NSPPE's cytotoxicity against the Vero normal kidney and caco cell lines. At lower doses, NSPPE was safe, but increasing concentrations gradually lethal against Vero normal kidney cell line. NSPPE was highly toxic to caco cells, with an IC50 339.76±13.9 µg mL-1. Fish kofta samples treated with NSPPE were completely free of Escherichia coli and Staphylococcus aureus after 8 weeks of storage. This study demonstrates that NSPPE may improve fish food safety and shelf life as a natural preservative

GROWTH, YIELD AND YIELD COMPONENT OF INOCULATED CHICKPEA AND FABA BEAN PLANTS AS AFFECTED BY USING METHYLOTROPHIC BACTERIA

Two pot experiments were performed in Giza research station, AgriculturalResearch Center (ARC) using 2 isolates of Pink-Pigmented FacultativelyMethylotrophic bacteria (PPFMs) originated from chickpea and Faba bean. Foliarapplication with PPFM isolates were conjugated with specific rhizobial inoculumand N-fertilization (15 Kg N Fed-1). Nodulation status, nitrogen fixation and growthyield and yield component were recorded. Results clearly indicated that Chickpeawas superior in its response to foliar application with PPFM.C. As it gave higherrecords of number and dry weight of nodules, dry matter and N-content of plants ascompared to Faba bean. A field experiment was also conducted in sandy loam soil atSouth EL-Tahreer province to investigate the effect of foliar application withPPFM.C strain + specific Rhizobia and N-fertilization on nodulation, growth andyield of chickpea legume plants. Results indicated that foliar application withPPFM.C in the presence of specific rhizobial inoculation scored significant increasesin economic turnover of chickpea in the range of 21-32% as compared to Nfertilizationat rate 50 Kg N Fed-1. Foliar application with 5 L Fed-1 in the presenceof 15 Kg N Fed-1 and specific rhizobial inoculation led to an increase of 518 kg fed -1productivity of seed yield , with economic turnover of 2491 L.E

REUSE OF WASTE DRAINAGE WATER AFTER ITS TREATMENT USING PGPRS TO IRRIGATE SOME HORTICULTURAL CROPS

Natural sources of water are limited in Egypt. The rapidly growing populations necessitate continuous expansion of the cultivated area. This means an increase of the gap between the demands of water for irrigation and the limited water supply. Looking for other sources such as low quality water like (industrial effluent, drainage and sewage) must be used in irrigation of some garden and wood plants. This investigation was conducted on agricultural drainage wastewater from El Mohete drain (Marioteya Canal) west of Cairo; the samples were collected from different places during (Summer and Winter seasons). The wastewater contaminated with pathogenic microorganisms, the excess of fertilizers (inorganic & organic), heavy metals, and the residuals of pesticides. Plant Growth Promoting Rhizobacteria (PGPR) could remediate the wastewater as biological bioremediation to remove some pollutants such as pathogenic microorganisms, heavy metals and pesticides. Chemical remediation was used as nitrification inhibitor to stop transformation of ammonia to nitrate. This work was conducted to study the ability of PGPR strains e.g. Bacillus megaterium, Bacillus subtillis, Bacillus circulans, Paenibacillus polymyxa, Pseudomonas floresense, Serratia sp. and Azotobacter chroococcum 5, 9 and 23 to treat the drainage water for irrigation the Mentha viridis cv. and Gladiolas grandiflorus cv. plants.                 Two pot experiments were conducted in greenhouse. The treatments were applied as follows; Natural water, treated water and drainage water to irrigate the plant. Use the PGPR as inoculants and thiourea as nitrification inhibitor, Heavy metal treated was (Copper, Cobalt, Zinc, Cadmium and Mercury) the result showed us heavy metals removal by PGPR from drainage water. The characterizations of PGPRs as shown in the obtained results are they could enhance plant growth by using their own metabolism (solublizing phosphate, producing hormones or fixing nitrogen) as well as correlation of them with the potenit of effects on the growth of plants in unfavorable conditions in order to improve the efficiency of phytoremdiation of contarinated soils. The removal of heavy metals and the elimination of pesticides residues were markedly noticed in this investigation. Results also confirmed the ability of PGPRs in suppressing the effect of pathogenic bacteria like Salmonellasp and E.coli. These abilities are of great importance in terms of plant and soil health. Consequently, the role of PGPRs bacteria associated with plant rhizosphere in remediation of water and soil contaminations due to its biochemical activity and thus, stimulate plant growth is a great important subject in phytoremediation process nowadays

ISOLATION, PURIFICATION AND IDENTIFICATION OF SOME MICROORGANISMS PRODUCE PLANT GROWTH PROMOTING SUBSTANCES (METHYLOTROPHIC BACTERIA)

Recently, the potential economical importance of the methylotrophic bacteria encouraged the isolation of this group. In the present study five Egyptian isolates were obtained from green leaves surface of legume plants named PPFM.C (ChickPea), PPFM.Ph (Common bean), PPFM.F (Faba bean), PPFM.P (Peanut) and PPFM.S (Soybean) ,to study their general characters which belonging to  methylotrophic bacteria. Morphological studies indicate that all isolates were short rods, gram negative and motile. All Physiological studies to the isolates gave the same results except PPFM.F which could not grow in peptone medium. All isolates were sensitive to Kanamycin but they were resistant to Erythromycin. There was a great range in the ability of the isolates to grow on different sodium chloride concentrations indicating that PPFM.Ph grew well in 5 % sodium chloride, and they were able to excrete and produce cytokinin. Molecular biology studies indicated that there was a great similarity between PPFM.C and PPFM.Ph (99.34%). Identification was carried out to the5 isolates, PPFM.F may be related to Methylobacterium mesophilicum, PPFM.P may be related to M. fujisawaense and PPFM.Ph, PPFM.C and PPFM.S were related to M. radiotolerans

Improving the productivity and quality of black cumin (Nigella sativa) by using Azotobacter as N2 biofertilizer

AbstractThirty one rhizosphere soil samples were collected from different gavernorates and localities cultivated with different standing crops. Samples were used for the isolation of N2-fixer Azotobacter sp. isolates. The purified isolates were identified as Azotobacter chroococcum. The purified isolates were tested for their N2 fixation activity, phosphates dissolving ability, production of plant growth promoting substances, exopolymer secretion, siderophores production, salicylic acid formation, and some enzymatic production. Out of these purified isolates namely Azo.4, Azo.5, Azo.9 and Azo.23 found to be more significant in the production of the aforementioned activities as compared with the other purified isolates. The four purified isolates were tested for some biochemical activities (hormonal activity and enzymes production) and used to prepare the effective microbial inoculants for black cumin (Nigella sativa) seeds. Results show that mixed inoculation with the four biofertilizer strains and using half dose of recommended N2-fertilizer enhanced the densities of the total microbial microflora, phosphate dissolving bacteria, azotobacters colonization, CO2 evolution in the rhizosphere of the inoculated plants and plant growth features in comparison with uninoculated plants (control). The effect of the crude oils of the produced black cumin seeds on some human pathogenic bacteria was studied. It was found that the crud fixed oil extracted from the seeds of plants has powerful antibacterial properties against this diverse genus of bacteria. However, the influence was different and depending on the tested bacterial strain