Consistent Paternity Skew through Ontogeny in Peron's Tree Frog (Litoria peronii)

BackgroundA large number of studies in postcopulatory sexual selection use paternity success as a proxy for fertilization success. However, selective mortality during embryonic development can lead to skews in paternity in situations of polyandry and sperm competition. Thus, when assessment of paternity fails to incorporate mortality skews during early ontogeny, this may interfere with correct interpretation of results and subsequent evolutionary inference. In a previous series of in vitro sperm competition experiments with amphibians (Litoria peronii), we showed skewed paternity patterns towards males more genetically similar to the female.Methodology/Principal FindingsHere we use in vitro fertilizations and sperm competition trials to test if this pattern of paternity of fully developed tadpoles reflects patterns of paternity at fertilization and if paternity skews changes during embryonic development. We show that there is no selective mortality through ontogeny and that patterns of paternity of hatched tadpoles reflects success of competing males in sperm competition at fertilization.Conclusions/SignificanceWhile this study shows that previous inferences of fertilization success from paternity data are valid for this species, rigorous testing of these assumptions is required to ensure that differential embryonic mortality does not confound estimations of true fertilization success.<br /

Quantitative genetics of breeding coloration in sand lizards; genic capture unlikely to maintain additive genetic variance

Sexual selection on fitness-determining traits should theoretically erode genetic variance and lead to low heritability. However, many sexually selected traits maintain significant phenotypic and additive genetic variance, with explanations for this “lek paradox” including genic capture due to condition-dependence, and breaks on directional selection due to environmental sources of variance including maternal effects. Here we investigate genetic and environmental sources of variance in the intrasexually selected green badge of the sand lizard (Lacerta agilis). The badge functions as a cue to male fighting ability in this species, and male–male interactions determine mate acquisition. Using animal models on a pedigree including three generations of males measured over an extensive 9-year field study, we partition phenotypic variance in both badge size and body condition into additive genetic, maternal, and permanent environmental effects experienced by an individual over its lifespan. Heritability of badge size was 0.33 with a significant estimate of underlying additive genetic variance. Body condition was strongly environmentally determined in this species and did not show either significant additive genetic variance or heritability. Neither badge size nor body condition was responsive to maternal effects. We propose that the lack of additive genetic variance and heritability of body condition makes it unlikely that genic capture mechanisms maintain additive genetic variance for badge size. That said, genic capture was originally proposed for male traits under female choice, not agonistic selection. If developmental pathways generating variance in body condition, and/or the covarying secondary sex trait, differ between inter- and intrasexual selection, or the rate at which their additive genetic variance or covariance is depleted, future work may show whether genic capture is largely restricted to intersexual selection processes.publishedVersio

Degrees of change: between and within population variation in thermal reaction norms of phenology in a viviparous lizard

As the earth warms, populations will be faced with novel environments to which they may not be adapted. In the short term, populations can be buffered against the negative effects, or maximize the beneficial effects, of such environmental change via phenotypic plasticity and, in the longer term, via adaptive evolution. However, the extent and direction of these population-level responses will be dependent on the degree to which responses vary among the individuals within them (i.e., within population variation in plasticity), which is, itself, likely to vary among populations. Despite this, we have estimates of among-individual variation in plastic responses across multiple populations for only a few systems. This lack of data limits our ability to predict the consequences of environmental change for population and species persistence accurately. Here, we utilized a 16-yr data set from climatically distinct populations of the viviparous skink Niveoscincus ocellatus tracking over 1,200 litters from more than 600 females from each population to examine inter- and intrapopulation variability in the response of parturition date to environmental temperature. We found that these populations share a common population-mean reaction norm but differ in the degree to which reaction norms vary among individuals. These results suggest that even where populations share a common mean-level response, we cannot assume that they will be affected similarly by altered environmental conditions. If we are to assess how changing climates will impact species and populations accurately, we require estimates of how plastic responses vary both among and within populations.publishedVersio

Sex reversal explains some, but not all, climate-mediated sex ratio variation within a viviparous reptile

Evolutionary transitions in sex-determining systems have occurred frequently yet understanding how they occur remains a major challenge. In reptiles, transitions from genetic to temperature-dependent sex determination can occur if the gene products that determine sex evolve thermal sensitivity, resulting in sex-reversed individuals. However, evidence of sex reversal is limited to oviparous reptiles. Here we used thermal experiments to test whether sex reversal is responsible for differences in sex determination in a viviparous reptile, Carinascincus ocellatus, a species with XY sex chromosomes and population-specific sex ratio response to temperature. We show that sex reversal is occurring and that its frequency is related to temperature. Sex reversal was unidirectional (phenotypic males with XX genotype) and observed in both high- and low-elevation populations. We propose that XX-biased genotypic sex ratios could produce either male- or female-biased phenotypic sex ratios as observed in low-elevation C. ocellatus under variable rates of XX sex reversal. We discuss reasons why sex reversal may not influence sex ratios at high elevation. Our results suggest that the mechanism responsible for evolutionary transitions from genotypic to temperature-dependent sex determination is more complex than can be explained by a single process such as sex reversal

A comparative study on wear and corrosion behaviour of tungsten carbide-nickel and tungsten carbide-cobalt high velocity oxy-fuel (HVOF) for carbon steel blade

Nowadays, the demand of high wear and corrosion resistance of the components in various industry is increasing from time to time. Therefore, high velocity oxy-fuel (HVOF) thermal spray was introduced to protect machine components from wear and corrosion, to restore worn components and to improve the durability of the components. HVOF is one of the process of depositing a material layer over a base metal or substrate with characteristics of high flame velocity and moderate temperature. The main purpose of this present study is to characterize the structure of the tungsten carbide 10 wt.% nickel (WC-10Ni) and tungsten carbide 12 wt.% w cobalt (WC-12Co) coating deposited by means of HVOF thermal spray onto a continuous digester (CD) blade that made up from carbon steel. The morphology and chemical composition of the coating were characterized by scanning electron microscope (SEM), electron dispersive spectrometer (EDS), and x-ray diffraction (XRD). The hardness test was carried out by using Vickers micro-hardness tester with load of 490.3 mN (0.05 HV). The wear and corrosion behavior and mechanism for both coatings was compared. Three body wear test was carried out in term of weight loss and electrochemical test was performed in acidic media (mixture of sulfuric acid, H2SO4 and ilmenite) to obtain the corrosion rate of the coating. From the result, it shows that WC-12Co coating has finer grain size that is around 2.3 μm. WC-12Co has higher wear resistance due to high volume friction, low mean free path, high hardness and lower porosity distribution compared to WC-10Ni. Besides, the formation of secondary phase, W2C also affected the hardness of both coating, where this phase is harder than WC phase. For corrosion test, WC-12Co shows good corrosion resistance with small differences of corrison rate with WC-10Ni, that is only 0.7016 mm/y. As a conclusion, WC-12Co HVOF coating shows high potential on replacement of CD blade

Engaging students through authentic research experiences

Student involvement is vital to engagement, making learning experiences more meaningful, and can be enriched by student-faculty interactions and diversity of learning environments (Smith et al. 2005). Experiential learning, in particular ‘hands on’ opportunities, can enhance undergraduate experiences and engender increased enthusiasm for the subject, as well as confidence on the part of students that they are being adequately prepared for science-related careers or postgraduate study (Gawel and Greengrove 2005). At the same time, quantifying the positive influence of student engagement with learning through authentic research experiences can be difficult (Griffiths 2004). Despite curricula emphasizing student-led enquiry, some students still struggle to find relevance and excitement in their undergraduate courses. Working from the Garnett and Holmes’ (1995) model of how both students and academics can benefit from research, we have designed an incremental suite of learning activities across the three years of the zoology undergraduate curriculum that extends and enhances the student experience. The aims of the program are to extend and challenge our undergraduate students by exposing them to current ‘hot topics’ in zoological research; enhance students’ appreciation of methodology, philosophy and outcomes of scientific research; enthuse students about the exciting research being carried out in zoology in Tasmania and encourage them to think of themselves as the researchers of the future. Here we describe several components of this program (summarised in Figure 1) which have been implemented and evaluated via student feedback

Diversity of reptile sex chromosome evolution revealed by cytogenetic and linked-read sequencing

Reptile sex determination is attracting much attention because the great diversity of sex-determination and dosage compensation mechanisms permits us to approach fundamental questions about mechanisms of sex chromosome turnover. Recent studies have made significant progress in better understanding diversity and conservation of reptile sex chromosomes, with however no reptile master sex determination genes identified. Here we describe an integrated genomics and cytogenetics pipeline, combining probes generated from the microdissected sex chromosomes with transcriptome and genome sequencing to explore the sex chromosome diversity in non-model Australian reptiles. We tested our pipeline on a turtle, two species of geckos, and a monitor lizard. Genes identified on sex chromosomes were compared to the chicken genome to identify homologous regions among the four species. We identified candidate sex determining genes within these regions, including conserved vertebrate sex-determining genes pdgfa, pdgfra amh and wt1, and demonstrated their testis or ovary-specific expression. All four species showed gene-by-gene rather than chromosome-wide dosage compensation. Our results imply that reptile sex chromosomes originated by independent acquisition of sex-determining genes on different autosomes, as well as translocations between different ancestral macro- and microchromosomes. We discuss the evolutionary drivers of the slow differentiation and turnover of reptile sex chromosomes

UV-Deprived Coloration Reduces Success in Mate Acquisition in Male Sand Lizards (Lacerta agilis)

Background: Recent work on animal signals has revealed a wide occurrence of UV signals in tetrapods, in particular birds, but also in lizards (and perhaps other Squamate reptiles). Our previous work on the Swedish sand lizard (Lacerta agilis) has verified, both in correlative selection analyses in the wild and with laboratory and field experiments, the importance of the green ‘badge ’ on the body sides of adult males for securing mating opportunities, probably mostly through deterring rival males rather than attracting females. The role of UV in communication has, however, never been examined. Methodology/Principal Findings: Here we show that when measured immediately after spring skin shedding, there is also signaling in the UV. By UV-depriving the signal (reflectance) with sun block chemicals fixated with permeable, harmless spray dressing, we show that males in the control group (spray dressing only) had significantly higher success in mate acquisition than UV-deprived males. Conclusions/Significance: These results suggest that at least two colour traits in sand lizards, badge area and UV, contribute to rival deterrence and/or female choice on UV characters, which elevates success in mate acquisition in UV intact male sand lizards