Nitrate and Inhibition of Ruminal Methanogenesis : Microbial Ecology, Obstacles, and Opportunities for Lowering Methane Emissions from Ruminant Livestock

Acknowledgments CY was supported by a scholarship from the China Scholarship Council. IC was supported by the SRUC International Engagement Strategy Fund. The nitrate project was funded by EBLEX, a Division of the Agriculture and Horticulture Development Board. RINH and SRUC are funded by the Rural and Environment Science and Analytical Services Division (RESAS) of the Scottish Government.Peer reviewedPublisher PD

Efficient Universal Noiseless Source Codes

Although the existence of universal noiseless variable-rate codes for the class of discrete stationary ergodic sources has previously been established, very few practical universal encoding methods are available. Efficient implementable universal source coding techniques are discussed in this paper. Results are presented on source codes for which a small value of the maximum redundancy is achieved with a relatively short block length. A constructive proof of the existence of universal noiseless codes for discrete stationary sources is first presented. The proof is shown to provide a method for obtaining efficient universal noiseless variable-rate codes for various classes of sources. For memoryless sources, upper and lower bounds are obtained for the minimax redundancy as a function of the block length of the code. Several techniques for constructing universal noiseless source codes for memoryless sources are presented and their redundancies are compared with the bounds. Consideration is given to possible applications to data compression for certain nonstationary sources

Excited state baryon spectroscopy from lattice QCD

We present a calculation of the Nucleon and Delta excited state spectrum on dynamical anisotropic clover lattices. A method for operator construction is introduced that allows for the reliable identification of the continuum spins of baryon states, overcoming the reduced symmetry of the cubic lattice. Using this method, we are able to determine a spectrum of single-particle states for spins up to and including J = 7/2, of both parities, the first time this has been achieved in a lattice calculation. We find a spectrum of states identifiable as admixtures of SU(6) x O(3) representations and a counting of levels that is consistent with the non-relativistic $qqq$ constituent quark model. This dense spectrum is incompatible with quark-diquark model solutions to the "missing resonance problem" and shows no signs of parity doubling of states.Comment: 29 pages, 18 figure

The Distribution of Metallicity in the IGM at z~2.5: OVI and CIV Absorption in the Spectra of 7 QSOs

We present a direct measurement of the metallicity distribution function for the high redshift intergalactic medium. We determine the shape of this function using survival statistics, which account for both detections and non-detections of OVI and CIV associated with HI absorption in quasar spectra. Our OVI sample probes the metal content of ~50% of all baryons at z~2.5. We find a median intergalactic abundance of [O,C/H]=-2.82; the differential abundance distribution is approximately lognormal with mean ~-2.85 and \sigma=0.75 dex. Some 60-70% the Lya forest lines are enriched to observable levels ([O,C/H]>-3.5) while the remaining ~30% of the lines have even lower abundances. Thus we have not detected a universal metallicity floor as has been suggested for some Population III enrichment scenaria. In fact, we argue that the bulk of the intergalactic metals formed later than the first stars that are thought to have triggered reionization. We do not observe a significant trend of decreasing metallicity toward the lower density IGM, at least within regions that would be characterized as filaments in numerical simulations. However, an [O/H] enhancement may be present at somewhat high densities. We estimate that roughly half of all baryons at these redshifts have been enriched to [O/H]>=-3.5. We develop a simple model for the metallicity evolution of the IGM, to estimate the chemical yield of galaxies formed prior to z~2.5. We find that the typical galaxy recycled 0.1-0.4% of its mass back into the IGM as heavy elements in the first 3 Gyr after the Big Bang.Comment: 23 pages in emulateapj, 19 figures. Accepted to ApJ, pending review of new changes. Revised comparison between our results and Schaye et al (2003

Computer control of a scanning electron microscope for digital image processing of thermal-wave images

Using a recently developed technology called thermal-wave microscopy, NASA Lewis Research Center has developed a computer controlled submicron thermal-wave microscope for the purpose of investigating III-V compound semiconductor devices and materials. This paper describes the system's design and configuration and discusses the hardware and software capabilities. Knowledge of the Concurrent 3200 series computers is needed for a complete understanding of the material presented. However, concepts and procedures are of general interest

High Sensitivity DNA Detection Using Gold Nanoparticle Functionalised Polyaniline Nanofibres

Polyaniline (PANI) nanofibres (PANI-NF) have been modified with chemically grown gold nanoparticles to give a nanocomposite material (PANI-NF–AuNP) and deposited on gold electrodes. Single stranded capture DNA was then bound to the gold nanoparticles and the underlying gold electrode and allowed to hybridise with a complementary target strand that is uniquely associated with the pathogen, Staphylococcus aureus (S. aureus), that causes mastitis. Significantly, cyclic voltammetry demonstrates that deposition of the gold nanoparticles increases the area available for DNA immobilisation by a factor of approximately 4. EPR reveals that the addition of the Au nanoparticles efficiently decreases the interactions between adjacent PANI chains and/or motional broadening. Finally, a second horseradish peroxidase (HRP) labelled DNA strand hybridises with the target allowing the concentration of the target DNA to be detected by monitoring the reduction of a hydroquinone mediator in solution. The sensors have a wide dynamic range, excellent ability to discriminate DNA mismatches and a high sensitivity. Semi-log plots of the pathogen DNA concentration vs. faradaic current were linear from 150 × 10−12 to 1 × 10−6 mol L−1 and pM concentrations could be detected without the need for molecular, e.g., PCR or NASBA, amplification

Observations of Chemically Enriched QSO Absorbers near z ~ 2.3 Galaxies: Galaxy-Formation Feedback Signatures in the IGM

We present a study of galaxies and intergalactic gas toward the z=2.73 quasar HS1700+6416, to explore the effects of galaxy formation feedback on the IGM. Our observations and ionization simulations indicate that the volume within 100-200 h_71^{-1} physical kpc of high-redshift galaxies contains very small, dense, and metal-rich absorption-line regions. These systems often contain shock-heated gas seen in OVI, and may exhibit [Si/C] abundance enhancements suggestive of Type II supernova enrichment. We argue that the absorbers resemble thin sheets or bubbles, whose physical properties can be explained with a simple model of radiatively efficient shocks propegating through the IGM. Their high metallicities suggest that these shocks are being expelled from--rather than falling into--star forming galaxies. There is a dropoff in the IGM gas density at galaxy impact parameters beyond ~300 physical kpc that may trace boundaries of gas structures where the galaxies reside. The local heavy-element enhancement covers 100-200 kpc; beyond this the observed abundances blend into the general IGM. Supernova-driven winds or dynamical stripping of interstellar gas appears to affect the IGM near massive galaxies, even at R>~100 kpc. However, these feedback systems represent only a few percent of the Lya forest mass at z~2.5. Their mass could be larger if the more numerous metal-poor CIV systems at >~200 kpc are tepid remnants of very powerful winds. Based on present observations it is not clear that this scenario is to be favored over one involving pre-enrichment by smaller galaxies at z>~6.Comment: Accepted for publication in the Astrophysical Journal. 26 pages emulateapj, incl. 5 pages tables, 15 figure

Lexicographically-ordered constraint satisfaction problems

We describe a simple CSP formalism for handling multi-attribute preference problems with hard constraints, one that combines hard constraints and preferences so the two are easily distinguished conceptually and for purposes of problem solving. Preferences are represented as a lexicographic order over complete assignments based on variable importance and rankings of values in each domain. Feasibility constraints are treated in the usual manner. Since the preference representation is ordinal in character, these problems can be solved with algorithms that do not require evaluations to be represented explicitly. This includes ordinary CSP algorithms, although these cannot stop searching until all solutions have been checked, with the important exception of heuristics that follow the preference order (lexical variable and value ordering). We describe relations between lexicographic CSPs and more general soft constraint formalisms and show how a full lexicographic ordering can be expressed in the latter. We discuss relations with (T)CP-nets, highlighting the advantages of the present formulation, and we discuss the use of lexicographic ordering in multiobjective optimisation. We also consider strengths and limitations of this form of representation with respect to expressiveness and usability. We then show how the simple structure of lexicographic CSPs can support specialised algorithms: a branch and bound algorithm with an implicit cost function, and an iterative algorithm that obtains optimal values for successive variables in the importance ordering, both of which can be combined with appropriate variable ordering heuristics to improve performance. We show experimentally that with these procedures a variety of problems can be solved efficiently, including some for which the basic lexically ordered search is infeasible in practice

Experiences with HPTN 067/ADAPT Study-Provided Open-Label PrEP Among Women in Cape Town: Facilitators and Barriers Within a Mutuality Framework.

Placebo-controlled trials of pre-exposure prophylaxis (PrEP) have reported challenges with study-product uptake and use, with the greatest challenges reported in studies with young women in sub-Saharan Africa. We conducted a qualitative sub-study to explore experiences with open-label PrEP among young women in Cape Town, South Africa participating in HTPN 067/Alternative Dosing to Augment Pre-Exposure Prophylaxis Pill Taking (ADAPT). HPTN 067/ADAPT provided open label oral FTC/TDF PrEP to young women in Cape Town, South Africa who were randomized to daily and non-daily PrEP regimens. Following completion of study participation, women were invited into a qualitative sub-study including focus groups and in-depth interviews. Interviews and groups followed a semi-structured guide, were recorded, transcribed, and translated to English from isiXhosa, and coded using framework analysis. Sixty of the 179 women enrolled in HPTN 067/ADAPT participated in either a focus group (six groups for a total of 42 participants) or an in-depth interview (n = 18). This sample of mostly young, unmarried women identified facilitators of and barriers to PrEP use, as well as factors influencing study participation. Cross-cutting themes characterizing discourse suggested that women placed high value on contributing to the well-being of one's community (Ubuntu), experienced a degree of skepticism towards PrEP and the study more generally, and reported a wide range of approaches towards PrEP (ranging from active avoidance to high levels of persistence and adherence). A Mutuality Framework is proposed that identifies four dynamics (distrust, uncertainty, alignment, and mutuality) that represent distinct interactions between self, community and study and serve to contextualize women's experiences. Implications for better understanding PrEP use, and non-use, and intervention opportunities are discussed. In this sample of women, PrEP use in the context of an open-label research trial was heavily influenced by underlying beliefs about safety, reciprocity of contributions to community, and trust in transparency and integrity of the research. Greater attention to factors positioning women in the different dynamics of the proposed Mutuality Framework could direct intervention approaches in clinical trials, as well as open-label PrEP scale-up