Bioreduction of silver nanoparticles from aerial parts of Euphorbia hirta L. (EH-ET) and its potent anticancer activities against neuroblastoma cell lines

Euphorbia hirta L. (Family: Euphorbiaceae) is a versatile medicinal plant and enriched with novel bioactive molecules and possess broad-spectrum pharmacological actions. Present work is aiming to synthesis and characterize of silver nanoparticles (AgNPs) by bioreduction method an using ethanolic extract of aerial parts of Euphorbia hirta L. (EH-ET). The synthesized AgNPs observed by a color change of source solution (as AgNPs) and further confirmed by the UV-Visible spectroscopic technique. The AgNPs synthesized were characterized by Scanning Electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR) and Zeta potential analysis. The synthesized AgNPs are polydispersed and some places it’s agglomerated. The particle size EH-ET silver nanoparticles were analyzed using Beckman coulter particle size analyzer (DelsaTM Nano common). The average size of the particles size noted in 2.9-206.3 nm. Anticancer activity of EH-ET Silver nanoparticles was tested against neuroblastoma cells (SH-SY5Y) and breast cancer cells (SH-SY5Y) and cytotoxicity were tested in vero cells by MTT assay. The preliminary confirmation of the synthesized AgNPs by the present method was made by the appearance of reddish brown color and the visible absorption peak at 429.5 nm. SEM image revealed that AgNPs synthesized were spherical in shape and silver nanoparticles were in the size range of 2.9 to 206.3 nm. FT-IR spectra showed the peaks corresponding to functional groups C=O, -C=C and -OH, which actively participated in bio-reduction and subsequent stabilization reactions in the synthesis of AgNPs. The obtained nanoparticles showed promising anticancer activity against neuroblastoma cells (SH-SY5Yc) and breast cancer cells (MCF-7) with IC50 values of 29.85 and 335 µg/mL, respectively. Whereas, the nanoparticles did not show any activity against vero cell lines. The synthesized silver nanoparticles using an ethanolic extract of Euphorbia hirta L. would be helpful for the preparation of potent cytotoxicity agents to destroy cancer cells

Opportunities and challenges of investing in Indian real estate

Thesis (S.M.)--Massachusetts Institute of Technology, Program in Real Estate Development in Conjunction with the Center for Real Estate , 2009.This electronic version was submitted by the student author. The certified thesis is available in the Institute Archives and Special Collections.Cataloged from student submitted PDF version of thesis.Includes bibliographical references (p. 125-128).In recent years, global real estate investment has become an important component of efficient global mixed asset portfolios. Although these investments carry increased political, regulatory and currency risk, international real estate investment has been on the rise. Compelling macroeconomic and demographic trends along with improvements in structural and regulatory conditions and investment in infrastructure are driving strong real estate capital flows into the emerging markets. This thesis provides a study of the opportunities and challenges of investing in one emerging market, India. With a population of over 1 billion, India has been a major beneficiary of the "Globalization of Real Estate". This thesis identifies the opportunities in India that have caused global capital to flow into Indian real estate and the key factors driving Indian real estate. It explains the challenges of investing in Indian real estate and seeks to provide strategies for navigating the real estate landscape in India.by Kunal Wadhwani.S.M

Membrane-Active Peptides and the Clustering of Anionic Lipids

AbstractThere is some overlap in the biological activities of cell-penetrating peptides (CPPs) and antimicrobial peptides (AMPs). We compared nine AMPs, seven CPPs, and a fusion peptide with regard to their ability to cluster anionic lipids in a mixture mimicking the cytoplasmic membrane of Gram-negative bacteria, as measured by differential scanning calorimetry. We also studied their bacteriostatic effect on several bacterial strains, and examined their conformational changes upon membrane binding using circular dichroism. A remarkable correlation was found between the net positive charge of the peptides and their capacity to induce anionic lipid clustering, which was independent of their secondary structure. Among the peptides studied, six AMPs and four CPPs were found to have strong anionic lipid clustering activity. These peptides also had bacteriostatic activity against several strains (particularly Gram-negative Escherichia coli) that are sensitive to lipid clustering agents. AMPs and CPPs that did not cluster anionic lipids were not toxic to E. coli. As shown previously for several types of AMPs, anionic lipid clustering likely contributes to the mechanism of antibacterial action of highly cationic CPPs. The same mechanism could explain the escape of CPPs from intracellular endosomes that are enriched with anionic lipids

An open multi-physics framework for modelling wildland-urban interface fire evacuations

Fire evacuations at wildland-urban interfaces (WUI) pose a serious challenge to the emergency services, and are a global issue affecting thousands of communities around the world. This paper presents a multi-physics framework for the simulation of evacuation in WUI wildfire incidents, including three main modelling layers: wildfire, pedestrians, and traffic. Currently, these layers have been mostly modelled in isolation and there is no comprehensive model which accounts for their integration. The key features needed for system integration are identified, namely: consistent level of refinement of each layer (i.e. spatial and temporal scales) and their application (e.g. evacuation planning or emergency response), and complete data exchange. Timelines of WUI fire events are analysed using an approach similar to building fire engineering (available vs. required safe egress times for WUI fires, i.e. WASET/WRSET). The proposed framework allows for a paradigm shift from current wildfire risk assessment and mapping tools towards dynamic fire vulnerability mapping. This is the assessment of spatial and temporal vulnerabilities based on the wildfire threat evolution along with variables related to the infrastructure, population and network characteristics. This framework allows for the integration of the three main modelling layers affecting WUI fire evacuation and aims at improving the safety of WUI communities by minimising the consequences of wildfire evacuations

Monetary Policy, Regulation and Volatile Markets

Turmoil in financial markets causes reflection. Is monetary policy conducted in the most efficient way? Are regulatory and supervisory arrangements adequate when market volatility increases and financial institutions come under stress? In the present SUERF Study, we have collected the reflections by an outstanding group of top officials, researchers and observers. The editors are proud to be able to present their joint insights to SUERF readers. The papers were presented at the 27th SUERF Colloquium in Munich in June 2008: New trends in asset management: Exploring the implications.Financial markets, volatility, regulatory and supervisory arrangements, LATW, bubbles, monetary policy, asset prices, interest rate policy, LTCM, Basel II, MiFID, subprime, CDOs

Orthopaedic aspect of anatomy and radiology of proximal femur

Femoral pathology is common in relation to the orthopedic. There is complex anatomy of the proximal femur and hip joint. So, its knowledge regarding anatomy and radiological correlation is necessary to the well-known fact for the orthopedics for the routine day to day practice. This review article briefly illustrates important anatomical and radiological aspect of the proximal femur

Monetary Policy, Regulation and Volatile Markets

Turmoil in financial markets causes reflection. Is monetary policy conducted in the most efficient way? Are regulatory and supervisory arrangements adequate when market volatility increases and financial institutions come under stress? In the present SUERF Study, we have collected the reflections by an outstanding group of top officials, researchers and observers. The editors are proud to be able to present their joint insights to SUERF readers. The papers were presented at the 27th SUERF Colloquium in Munich in June 2008: New trends in asset management: Exploring the implications