A new family of boundary-domain integral equations for the Dirichlet Problem of the diffusion equation in nhomogeneous media with H −1 (Ω) source term on Lipschitz Domains

The interior Dirichlet boundary value problem for the diffusion equation in nonhomogeneous media is reduced to a system of Boundary-Domain Integral Equations (BDIEs) employing the parametrix obtained in 1 different from2. We further extend the results obtained in 1 for the mixed problem in a smooth domain with L²(Ω) hand side to Lipschitz domains and PDE right-hand in the Sobolev space H¯(Ω), where neither the classical nor the canonical co-normal derivatives are well defined. Equivalence between the system of BDIEs and the original BVP is proved along with their solvability and solution uniqueness in appropriate Sobolev spaces

Evaluation of theoretical uncertainties in parity-violating electron scattering from nucleons and nuclei

Parity-violating polarized electron scattering from nucleons and nuclei provides an excellent tool to extract valuable information on nuclear and nucleon structure, as well as to determine Standard Model couplings and higher-order radiative corrections. As measurements become more precise, theoretical models should improve accordingly in order to exploit the experimental data fully in extracting meaningful information. At the same time, it is crucial that theoretical evaluations come with realistic estimations of the corresponding theoretical uncertainties to establish that the precision reached in the measurements is not compromised. Here we consider isospin mixing and the charge distribution in nuclei, and strangeness content of the nucleon together with its axial form factor.Junta de Andalucía FQM-16

Differentiation of a hyperthermophilic archaeon Pyrococcus sp. Strain Pikanate 5017, by arbitrarily primed PCR

Pyrococcus and Thermococcus are hyperthermophilic archaea in the order Thermococcales. Both genera are strictly anaerobes, gaining energy by fermentation of peptide and several carbohydrates at optimal temperature above 70 ° C. A Pyrococcus sp. strain Pikanate 5017 (PK 5017) was recently isolated from a hot spring in Northern Thailand. The strain PK 5017 is a valuable source of numerous genes encoding thermostable enzymes. Growth kinetics determined at various temperatures (75-105 ° C) indicates that strain PK5017 is a fast growing archaeon. An arbitrarily primed PCR (AP-PCR) technique was successfully applied to differentiate the genome sequences of six members of Thermococcales. Two single primers, ARB-1f (5’ ATGAG GACT GAAA CCATT 3’) and ARB-2f (5’ GTAAA ACGA CGGC CAGT 3’), are effective in producing polymorphisms of the PCR products at 3-10 ng of DNAs. The unique AP-PCR fingerprints distinguish the strain PK5017 from P. furiosus, P. horikoshii, P. abyssi, T. litoralis, and T. celer. The results indicate a clear distinction of genome sequences among Pyrococcus and Thermococcus genera.This work was supported by a grant from the Scientific Promotion and Development Fund, Faculty of Science, Silpakorn University (Grant number RGI 2553-06). JMG and MCP acknowledge the support from a CSIC movility program, PA1001993 and PA1002058, and the Andalusian Government Bio288Peer Reviewe

Identification and characterization of a freshwater Pyrococcus sp. strain PK 5017 and identification of pfu-like IS elements in Thermococcus sibiricus MM 739

12 pages, 7 figures, 1 table, 32 references.A strictly anaerobic, hyperthermophilic Pyrococcus sp. strain PK 5017 (PK 5017) was isolated from a freshwater hot spring in Thailand. Cells of strain PK 5017 are irregular cocci occurring singly and in pairs with a diameter range of 0.7-1.2 µm. Temperature, pH and NaCl concentration ranges for growth are 75-105 oC (opt. temp. = 95-100 oC), pH 5-7.8 (opt. pH = 7.2) and 2.5-IS-pfu-Ts1> and >IS-pfu-Ts2> are identified in the complete genome sequence of Thermococcus sibiricus MM 739. Pyrococcus sp. strain PK 5017 = Pyrococcus sp. strain Pikanate 5017 = JCM17043 = ATCC BAA-2246.This work was supported by the Scientific Promotion and Development Fund, Faculty of Science, Silpakorn University (RGI 2553-06) and Silpakorn University Research and Development Institute (SURDI 54/01/18 and SURDI 55/01/05). JMG and MCP acknowledge support from a CSIC movility program, PA1001993 and PA1002058, and the Andalusian Government Bio288 which included FEDER funds.Peer Reviewe

Fervidobacterium thailandense sp. nov., an extremely thermophilic bacterium isolated from a hot spring

4 páginas.-- 2 figuras.-- 19 referencias.-- Two supplementary tables and four supplementary figures are available with the online Supplementary Material.Strain FC2004T, a strictly anaerobic, extremely thermophilic heterotroph, was isolated from a hot spring in Thailand. Typical cells of strain FC2004T were rod shaped (0.5–0.6×1.1–2.5 µm) with an outer membrane swelling out over an end. Filaments (10–30 µm long) and membrane-bound spheroids containing two or more cells inside (3–8 µm in diameter) were observed. The temperature range for growth was 60–88°C (optimum 78–80°C), pH range was 6.5–8.5 (optimum pH 7.5) and NaCl concentration range was 0 to <5 g l−1 (optimum 0.5 g l−1). S0 stimulated growth yield. S2O3 2– and NO3 − did not influence growth. Glucose, maltose, sucrose, fructose, cellobiose, CM-cellulose and starch were utilized for growth. The membrane was composed mainly of the saturated fatty acids C16:0 and C18:0. The DNA G+C content was 45.8 mol%. The 16S rRNA gene sequence of strain FC2004T revealed highest similarity to species of the genus Fervidobacterium : F. pennivorans DSM 9078T (97–96 %), F. islandicum AW-1 (96 %), F. changbaicum CBS-1T (96 %), F. islandicum H21T (95 %), F. nodosum Rt17-B1T (95 %), F. riparium 1445tT (95 %) and F. gondwanense AB39T (93 %). Phylogenetic analysis of 16S rRNA gene sequences and average nucleotide identity analysis suggested that strain FC2004T represented a novel species within the genus Fervidobacterium , for which the name Fervidobacterium thailandense sp. nov. is proposed. The type strain is FC2004T (=JCM 18757T=ATCC BAA-2483T).Peer reviewe