ENRICHING THE CULTURE OF AMMONIA OXIDIZING BACTERIA FROM SOIL AND FISHPOND WITH BIO-FILTERS

A special group of microorganisms, which are called ammonia oxidizing bacteria (AOB), is capable to reduce ammonia-nitrogen from the ecosystem. However, this bacterium is slow growing and has a low yield capacity. Hence, the objective of this research was to grow the AOB using a support medium in the form of biofilters to reduce the ammonia-nitrogen. The enrichment cultures of this AOB were performed from the inoculum of common soil and effluent from fishpond. The experiment was conducted using two sets of flasks labelled Medium A and Medium B, respectively. Each medium contained three flasks of which Flasks 1 and 2 were inoculated with the common soil and effluent from a fishpond. Flask 3 was the control, which contained neither soil nor effluent from any fishpond or bio-filter. Then the ammonia-nitrogen and the formation of nitrite-nitrogen were measured. The results showed that the enrichment culture of the effluent of the fishpond had achieved a high formation of nitrite-nitrogen (450 µg/L) in comparison with the enrichment culture of the soil (360 µg/L). A high reduction of ammonia-nitrogen (50%) was obtained from the fishpond culture rather than the soil (40%). Secondly, a higher reduction of ammonia-nitrogen and production of nitrite-nitrogen were found in the bio-filter flasks against the controls (without bio-filter). In summary, it can be concluded that the bio-filter can be a reliable support to grow AOB

Escape from noisy intermittent repellers

Intermittent or marginally-stable repellers are commonly associated with a power law decay in the survival fraction. We show here that the presence of weak additive noise alters the spectrum of the Perron - Frobenius operator significantly giving rise to exponential decays even in systems that are otherwise regular. Implications for ballistic transport in marginally stable miscrostructures are briefly discussed.Comment: 3 ps figures include

Comparing ultra-high spatial resolution remote-sensing methods in mapping peatland vegetation

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Formulation and utilisation of spent anaerobic digestate fluids for the growth and product formation of single cell algal cultures in heterotrophic and autotrophic conditions

Spent anaerobically digested effluents of agricultural origin were collected and treated using membrane filtration to achieve three- large particle free- nutrient streams of N:P ratios of 16.53, 3.78 and 14.22. Three algal species were grown on these streams, achieving good levels of bioremediation of digester fluids simultaneously with biomass and associated end product formation. Nannochloropsis oceanica and Scenedesmus quadricuada, where proven highly effective in remediating the streams achieving ammonia and phosphate reduction over 60% while for Schizochytrium limacinum SR21 these serve as an ideal production medium for lipids and biomass reaching 16.70 w/w% and 1.42 g L-1 correspondingly.These processes thus provide treatment of sludge, avoiding the disposal problems by land spreading. The solid components are nutrient depleted but rich in organic matter as a soil enhancer, while the fluids rich in nutrients can be efficiently utilised for growth to generate high value materials of microalgae facilitating water reclamation

Utilising light-emitting diodes of specific narrow wavelengths for the optimization and co-production of multiple high-value compounds in Porphyridium purpureum

The effect of specific narrow light-emitting diode (LED) wavelengths (red, green, blue) and a combination of LED wavelengths (red, green and blue - RGB) on biomass composition produced by Porphyridium purpureum is studied. Phycobiliprotein, fatty acids, exopolysaccharides, pigment content, and the main macromolecules composition were analysed to determine the effect of wavelength on multiple compounds of commercial interest. The results demonstrate that green light plays a significant role in the growth of rhodophyta, due to phycobiliproteins being able to harvest green wavelengths where chlorophyll pigments absorb poorly. However, under multi-chromatic LED wavelengths, P. purpureum biomass accumulated the highest yield of valuable products such as eicosapentaenoic acid (~2.9 %DW), zeaxanthin (~586 μg g− 1 DW), β-carotene (397 μg g− 1 DW), exopolysaccharides (2.05 g/L-1), and phycobiliproteins (~ 4.8 % DW). This increased accumulation is likely to be the combination of both photo-adaption and photo-protection, under the combined specific wavelengths employed

Light Intensity and Nitrogen Concentration Impact on the Biomass and Phycoerythrin Production by Porphyridium purpureum

Several factors have the potential to influence microalgae growth. In the present study, nitrogen concentration and light intensity were evaluated in order to obtain high biomass production and high phycoerythrin accumulation from Porphyridium purpureum. The range of nitrogen concentrations evaluated in the culture medium was 0.075–0.450 g L−1 and light intensities ranged between 30 and 100 μmol m−2 s−1. Surprisingly, low nitrogen concentration and high light intensity resulted in high biomass yield and phycoerythrin accumulation. Thus, the best biomass productivity (0.386 g L−1 d−1) and biomass yield (5.403 g L−1) were achieved with NaNO3 at 0.075 g L−1 and 100 μmol m−2 s−1. In addition, phycoerythrin production was improved to obtain a concentration of 14.66 mg L−1 (2.71 mg g−1 of phycoerythrin over dry weight). The results of the present study indicate that it is possible to significantly improve biomass and pigment production in Porphyridium purpureum by limiting nitrogen concentration and light intensity

Is inhibition of kinase activity the only therapeutic strategy for LRRK2-associated Parkinson's disease?

Mutations in the leucine-rich repeat kinase 2 (LRRK2) gene are a common cause of familial Parkinson's disease (PD). Variation around the LRRK2 locus also contributes to the risk of sporadic PD. The LRRK2 protein contains a central catalytic region, and pathogenic mutations cluster in the Ras of complex protein C terminus of Ras of complex protein (mutations N1437H, R1441G/C and Y1699C) and kinase (G2019S and I2020T) domains. Much attention has been focused on the kinase domain, because kinase-dead versions of mutant LRRK2 are less toxic than kinase-active versions of the same proteins. Furthermore, kinase inhibitors may be able to mimic this effect in mouse models, although the currently tested inhibitors are not completely specific. In this review, we discuss the recent progress in the development of specific LRRK2 kinase inhibitors. We also discuss non-kinase-based therapeutic strategies for LRRK2-associated PD as it is possible that different approaches may be needed for different mutations