Comparative study of CXC chemokines modulation in brown trout (Salmo trutta) following infection with a bacterial or viral pathogen

Acknowledgements We would like to acknowledge Richard Paley, Tom Hill and Georgina Rimmer for their collaboration during brown trout infection challenges in CEFAS-Weymouth biosecurity facilities. Bartolomeo Gorgoglione, Stephen W. Feist and Nick G. H. Taylor were supported by a DEFRA grant (F1198).Peer reviewedPostprin

Nucleoside analogues from push-pull functionalized branched-chain pyranosides

The reaction of methyl 4,6-O-benzylidene-2-deoxy-α-D-erythro- hexopyranosid-3-ulose (1) with ethynylmagnesium bromide in tetrahydrofuran and subsequent trimethylsilylation yielded the methyl 4,6-O-benzylidene-2-deoxy-3-C- ethynyl-3-O-trimethylsilyl-α-D-ribo-hexopyranoside (3). Push-pull functionalization of 3 with N,N,N′,N′,N″,N″- hexamethylguanidinium chloride under basic conditions and following deprotection afforded the spiro{2,5-dihydro-3-dimethylamino-furan-2,8'-4',4'a,6',7',8',8'a- hexahydro-6'-methoxy-2'-phenyl-pyrano[3,2-d][1,3]dioxine}-5- ylidenemalononitrile (9). Furthermore, compound 1 reacted with N,N-dimethylformamide dimethylacetal to furnish methyl (E)-4,6-O-benzylidene-2- deoxy-2-dimethylaminomethylene-α-D-erythro-hexopyranosid-3-ulose (10). Treatment of 10 with methylhydrazine and amidines yielded (4S,5aR,8R,9aS)-2,5a, 6,9a-tetrahydro-4-methoxy-2-methyl-8-phenyl-4H-[1,3]dioxino[4',5':5,6]pyrano[4, 3-c]pyrazole (11a) and (2R,4aR,6S,10bS)-4,4a,6,10b-tetrahydro-6-methoxy-2- phenyl[1,3]dioxino[4',5':5,6]pyrano[4,3-d]pyrimidines 12, respectively. © 2006 Verlag der Zeitschrift für Naturforschung

Brass Band Ephraham Jones / music by Geo W. Meyer; words by Joe Goodwin

Cover: drawing of Ephraham Jones dancing in the foreground, while a marching band performs in the background; Publisher: Leo Feist Inc. (New York)https://egrove.olemiss.edu/sharris_c/1016/thumbnail.jp

Immune response modulation upon sequential heterogeneous co-infection with Tetracapsuloides bryosalmonae and VHSV in brown trout (Salmo trutta)

This work was supported by DEFRA [grant number F1198] in a joint project between SFIRC in Aberdeen, Scotland and CEFAS laboratory in Weymouth, England. Further funding to present this work at international conferences was granted to BG by European Association of Fish Pathologists (EAFP), British Society for Immunology (BSI), Fisheries Society of the British Isles (FSBI), European Society of Veterinary Virology (ESVV), and Aberdeen University Principal’s Excellence Fund. JWH was supported by the BBSRC (BB/K009125/1).Peer reviewedPostprin

Anything Is Nice If It Comes From Dixieland / words by Grant Clarke, George W. Meyer, and Milton Ager

Cover: drawing of an African American man playing banjo on a riverbank; photo inset of a woman (see #162); Publisher: Leo Feist Inc. (New York)https://egrove.olemiss.edu/sharris_c/1165/thumbnail.jp

There\u27s A lot of Blue-Eyed-Marys down in Maryland / music by Geo W. Meyer; words by Jack Yellen and Milton Ager

Cover: a drawing of girls faces; Publisher: Leo Feist Inc. (New York)https://egrove.olemiss.edu/sharris_c/1175/thumbnail.jp

Anything is Nice if it Comes from Dixieland / music by Geo W. Meyer and Milton Ager ; words by Grant Clarke

Cover: drawing of an African American male playing a banjo, as a riverboat floats by in the background (see 321); Publisher: Leo Feist Inc. (New York)https://egrove.olemiss.edu/sharris_c/1158/thumbnail.jp

Förster resonance energy transfer within single chain nanoparticles

Single chain nanoparticles (SCNPs) are a highly versatile polymer architecture consisting of single polymer chains that are intramolecularly crosslinked. Currently, SCNPs are discussed as powerful macromolecular architectures for catalysis, delivery and sensors. Herein, we introduce a methodology based on Förster Resonance Energy Transfer (FRET) to evidence the folding of single polymer chains into SCNPs via fluorescence readout. We initially introduce a molecular FRET pair based on a bimane and nitrobenzoxadiazole (NBD) moiety and study its fluorescence properties in different solvents. We subsequently construct a low dispersity polymer chain carrying NBD units, while exploiting the bimane units for intramolecular chain collapse. Upon chain collapse and SCNP formation – thus bringing bimane and NBD units into close proximity – the SCNPs report their folded state by a strong and unambiguous FRET fluorescence signal. The herein introduced reporting of the folding state of SCNPs solely relies on an optical readout, opening avenues to monitoring SCNP folding without recourse to complex analytical methodologies