The effects of a high fat diet and voluntary wheel running on Cidea and Cidec protein expression in liver.

<p>Mice were fed a LFD or HFD and housed in standard cages or wheel running cages for weeks. Liver was excised and rapidly frozen in liquid nitrogen for subsequent Western blot analyses. <b>(A)</b> Representative immunoblots for Cidea and Cidec as well as β-actin as a loading control. Quantification of <b>(B)</b> Cidea and <b>(C)</b> Cidec protein, each normalized to β-actin. *, Denotes statistically significant difference from HFD-Vehicle. N = 5 mice per group.</p

CIDE protein expression in adipose tissue and liver from CON-SED, CON-WR, HFD-SED, and HFD-WR mice is related to measures of adiposity and insulin resistance.

<p>*Significant Pearson product-moment correlation coefficient, P value is ≤.05.</p><p>Tissues from 20 mice were used for the analysis.</p><p>CIDE protein expression in adipose tissue and liver from CON-SED, CON-WR, HFD-SED, and HFD-WR mice is related to measures of adiposity and insulin resistance.</p

The effects of a high fat diet and voluntary wheel running on insulin-assisted glucose tolerance.

<p>Mice received a simultaneous injection of glucose (2 mg/Kg) and insulin (2 U/Kg), and glucose was measured in blood collected from the tail vein at 0, 20, 40, and 60 minutes following the injection (Panel A). The area under the insulin-assisted glucose tolerance curve (AUC) was calculated from blood glucose values during the IAGTT (Panel B).*Denotes statistically significant difference from all other groups by Fisher’s LSD post-hoc test. The number of mice per group is 6–9.</p

The effects of a high fat diet and voluntary wheel running on Cidea and Cidec protein expression in adipose tissue.

<p>Mice were fed a LFD or HFD and housed in standard cages or wheel running cages for weeks. Adipose tissue was excised and rapidly frozen in liquid nitrogen for subsequent Western blot analyses. <b>(A)</b> Representative immunoblots for Cidea and Cidec as well as β-actin as a loading control. Quantification of <b>(B)</b> Cidea and <b>(C)</b> Cidec protein, each normalized to β-actin. *, Denotes statistically significant difference from HFD-Vehicle. N = 5 mice per group.</p

The effects of a high fat diet and voluntary wheel running on PPARγ mRNA expression in liver and adipose tissue.

<p>PPARγ gene expression for liver (Panel A) and epididymal white adipose tissue (Panel B), were normalized to GAPDH and expressed relative to LFD-SED values. ⁺Denotes statistically significant difference from LFD-SED and LFD-WR groups by Fisher’s LSD post-hoc test. The number of mice per group is 5.</p

The effects of a high fat diet and voluntary wheel running on Cidea and Cidec mRNA expression in liver and adipose tissue.

<p>Cidea and Cidec gene expression for liver (Panels A and B) and epididymal white adipose tissue (Panels C and D) were normalized to GAPDH and expressed relative to LFD-SED values. *Denotes statistically significant difference from all other groups by Fisher’s LSD post-hoc test. ⁺Denotes statistically significant difference from LFD-SED and LFD-WR groups by Fisher’s LSD post-hoc test. The number of mice per group is 4–5.</p

The effects of a high fat diet and voluntary wheel running on body mass, epididymal white adipose tissue (EWAT) mass, and caloric intake.

<p>⁺Significantly different from SED group on respective diet by Fisher’s LSD post-hoc test</p><p>*Significantly different from CON-SED group by Fisher’s LSD post-hoc test</p><p>The effects of a high fat diet and voluntary wheel running on body mass, epididymal white adipose tissue (EWAT) mass, and caloric intake.</p