Metabolites Profiling of Penicillium citrinum Recovered from Endophytic of Ramie (Boehmeria nivea) as a Potential Biocontrol Against Pathogenic Fungi

Endophytes are a potent source of bioactive compounds that mimic plant-based metabolites. Fungi Fusarium spp. and Clonostachys rosea have been identified as pathogenic microorganisms in ramie  (Boehmeria nivea). The antifungal test was carried out using the diffusion method and the MIC50 and Minimum Fungicidal Concentration (MFC) values using the tested microbial pathogens were Fusarium solani isolate 3248941, F. solani isolate Colpat-359, F. oxysporum isolate N-61-2, dan Clonostachys rosea strain B3042. Identification of secondary metabolites of the extract was carried out using GC-MS. The chromatogram of GC-MS analysis of this ethyl acetate extract (EA) showed seven dominant chemical compounds with various biological activities. Ethyl asetate extract of  P. citrinum showed inhibition zone ranged from 19.10–22.07  mm with strong-very strong category at  200 mg ml-1 concentration against the tested microbial pathogens. Results revealed that the significant MIC values were observed against F. solani isolate 3248941 and Clonostachys rosea strain B3042 by less than 6.3  mg ml-1 and against F. solani isolate Colpat-359 and F. oxysporum isolate N-61-2  with 12.5 mg ml-1 and 25 mg ml-1 respectively. Minimum Fungicidal concentrations (MFC) for EA of  P. citrinum were also reported against F. solani isolate 3248941 and C. rosea strain B3042, by 12.5 mg ml-1, whilst Fusarium solani isolate Colpat-359  by 25 mg ml-1 and Fusarium oxysporum isolate N-61-2 ,by 50 mg ml-1 respectively. Results of this study showed that P. citrinum, endophytic fungi of ramie, could be a promising source of compounds for antifungal agents

Karakterisasi Feno-Genotipik Kemampuan Hemolisa Isolat Staphylococcus aureus Asal Susu Kambing Mastitis dan Daging Ayam Segar

Abstract  Staphylococcus aureus (S. aureus) is a pathogenic bacterium with many virulence factors, such as toxins, immunomodulatory factors, and exoenzymes. Hemolysin is one of the essential toxins in the pathogenesis of S. aureus, with the action of lysing erythrocytes and other cells. This study aimed to determine the phenotypic and genotypic characteristics of S. aureus isolates from animal origin. Eight S. aureus isolates from goat mastitis and raw chicken meat phenotypically evaluated the ability to hemolyze sheep blood. At the same time, genotypic were determinated by detecting the hla and hlb genes. The results showed that most 87.5% (7/8) of S. aureus isolates had β-hemolysis phenotypic. PCR determination showed that all (8/8) isolates were positive for the α-toxin (hla) encoding gene, while half (4/8) of the isolates were positive for the β-toxin (hlb) encoding gene. The use of 5% defibrinated sheep blood agar plates is recommended to determine the hemolytic character of S. aureus tested. Keywords: Character; Hemolysis; S. aureus   Abstrak  Staphylococcus aureus (S. aureus) merupakan bakteri patogen yang memiliki banyak faktor virulensi seperti toksin, faktor imunomodulator, dan eksoenzim. Hemolisin merupakan salah satu toksin penting dalam patogenesis S. aureus, dengan aksi kerja melisiskan eritrosit dan sel lainnya. Penelitian ini bertujuan untuk mengetahui karakter hemolisa isolat S. aureus asal hewan secara fenotipik dan genotipik. Delapan isolat S. aureus asal susu kambing mastitis dan daging ayam segar dievaluasi secara fenotipik untuk melihat kemampuan menghemolisa darah domba, sedangkan determinasi genotipik dengan deteksi gen hla dan gen hlb. Hasil penelitian menunjukkan mayoritas 87.5% (7/8) isolat S. aureus memiliki fenotipik β-hemolisa. Determinasi PCR menunjukkan 100% (8/8) isolat positif gen penyandi α-toksin (hla), sedangkan 50% (4/8) isolat positif gen penyandi β-toksin (hlb). Penggunaan 5% defibrinated pelat agar darah domba lebih direkomendasikan untuk melihat karakter hemolisa S. aureus yang diuji. Kata kunci: Hemolisa; Karakter; S. aureu