First DNA barcode reference library for the identification of South American freshwater fish from the lower Paraná river

Valid fish species identification is essential for biodiversity conservation and fisheries management. Here, we provide a sequence reference library based on mitochondrial cytochrome c oxidase subunit I for a valid identification of 79 freshwater fish species from the Lower Paraná River. Neighbour-joining analysis based on K2P genetic distances formed non-overlapping clusters for almost all species with a ≥99% bootstrap support each. Identification was successful for 97.8% of species as the minimum genetic distance to the nearest neighbour exceeded the maximum intraspecific distance in all these cases. A barcoding gap of 2.5% was apparent for the whole data set with the exception of four cases. Withinspecies distances ranged from 0.00% to 7.59%, while interspecific distances varied between 4.06% and 19.98%, without considering Odontesthes species with a minimum genetic distance of 0%. Sequence library validation was performed by applying BOLDs BIN analysis tool, Poisson Tree Processes model and Automatic Barcode Gap Discovery, along with a reliable taxonomic assignment by experts. Exhaustive revision of vouchers was performed when a conflicting assignment was detected after sequence analysis and BIN discordance evaluation. Thus, the sequence library presented here can be confidently used as a benchmark for identification of half of the fish species recorded for the Lower Paraná River.Instituto de Limnología "Dr. Raul A. Ringuelet

Boosting Biomass Quantity and Quality by Improved Mixotrophic Culture of the Diatom Phaeodactylum tricornutum

Diatoms are photoautotrophic unicellular algae and are among the most abundant, adaptable, and diverse marine phytoplankton. They are extremely interesting not only for their ecological role but also as potential feedstocks for sustainable biofuels and high-value commodities such as omega fatty acids, because of their capacity to accumulate lipids. However, the cultivation of microalgae on an industrial scale requires higher cell densities and lipid accumulation than those found in nature to make the process economically viable. One of the known ways to induce lipid accumulation in Phaeodactylum tricornutum is nitrogen deprivation, which comes at the expense of growth inhibition and lower cell density. Thus, alternative ways need to be explored to enhance the lipid production as well as biomass density to make them sustainable at industrial scale. In this study, we have used experimental and metabolic modeling approaches to optimize the media composition, in terms of elemental composition, organic and inorganic carbon sources, and light intensity, that boost both biomass quality and quantity of P. tricornutum. Eventually, the optimized conditions were scaled-up to 2 L photobioreactors, where a better system control (temperature, pH, light, aeration/mixing) allowed a further improvement of the biomass capacity of P. tricornutum to 12 g/L

12C/13C isotopic ratios in red-giant stars of the open cluster NGC 6791

Carbon isotope ratios, along with carbon and nitrogen abundances, are derived in a sample of 11 red-giant members of one of the most metal-rich clusters in the Milky Way, NGC 6791. The selected red-giants have a mean metallicity and standard deviation of [Fe/H]=+0.39+-0.06 (Cunha et al. 2015). We used high resolution H-band spectra obtained by the SDSS-IV Apache Point Observatory Galactic Evolution Experiment (APOGEE). The advantage of using high-resolution spectra in the H-band is that lines of CO are well represented and their line profiles are sensitive to the variation of 12C/13C. Values of the 12C/13C ratio were obtained from a spectrum synthesis analysis. The derived 12C/13C ratios varied between 6.3 and 10.6 in NGC 6791, in agreement with the final isotopic ratios from thermohaline-induced mixing models. The ratios derived here are combined with those obtained for more metal poor red-giants from the literature to examine the correlation between 12C/13C, mass, metallicity and evolutionary status.Comment: Accepted for publication in MNRAS, 9 pages, 4 figures, 2 table

Au-Decorated Ce-Ti Mixed Oxides for Efficient CO Preferential Photooxidation

We investigated the photocatalytic behavior of gold nanoparticles supported on CeO2-TiO2 nanostructured matrixes in the CO preferential oxidation in H2-rich stream (photo-CO-PROX), by modifying the electronic band structure of ceria through addition of titania and making it more suitable for interacting with free electrons excited in gold nanoparticles through surface plasmon resonance. CeO2 samples with different TiO2 concentrations (0-20 wt %) were prepared through a slow coprecipitation method in alkaline conditions. The synthetic route is surfactant-free and environmentally friendly. Au nanoparticles (<1.0 wt % loading) were deposited on the surface of the CeO2-TiO2 oxides by deposition-precipitation. A benchmarking sample was also considered, prepared by standard fast coprecipitation, to assess how a peculiar morphology can affect the photocatalytic behavior. The samples appeared organized in a hierarchical needle-like structure, with different morphologies depending on the Ti content and preparation method, with homogeneously distributed Au nanoparticles decorating the Ce-Ti mixed oxides. The morphology influences the preferential photooxidation of CO to CO2 in excess of H2 under simulated solar light irradiation at room temperature and atmospheric pressure. The Au/CeO2-TiO2 systems exhibit much higher activity compared to a benchmark sample with a non-organized structure. The most efficient sample exhibited CO conversions of 52.9 and 80.2%, and CO2 selectivities equal to 95.3 and 59.4%, in the dark and under simulated sunlight, respectively. A clear morphology-functionality correlation was found in our systematic analysis, with CO conversion maximized for a TiO2 content equal to 15 wt %. The outcomes of this study are significant advancements toward the development of an effective strategy for exploitation of hydrogen as a viable clean fuel in stationary, automotive, and portable power generators

Chemical abundances and ages of the bulge stars in APOGEE high-velocity peaks

A cold high-velocity (HV, $\sim$ 200 km/s) peak was first reported in several Galactic bulge fields based on the APOGEE commissioning observations. Both the existence and the nature of the high-velocity peak are still under debate. Here we revisit this feature with the latest APOGEE DR13 data. We find that most of the low latitude bulge fields display a skewed Gaussian distribution with a HV shoulder. However, only 3 out of 53 fields show distinct high-velocity peaks around 200 km/s. The velocity distribution can be well described by Gauss-Hermite polynomials, except the three fields showing clear HV peaks. We find that the correlation between the skewness parameter ($h_{3}$) and the mean velocity ($\bar{v}$), instead of a distinctive HV peak, is a strong indicator of the bar. It was recently suggested that the HV peak is composed of preferentially young stars. We choose three fields showing clear HV peaks to test this hypothesis using the metallicity, [$\alpha$/M] and [C/N] as age proxies. We find that both young and old stars show HV features. The similarity between the chemical abundances of stars in the HV peaks and the main component indicates that they are not systematically different in terms of chemical abundance or age. In contrast, there are clear differences in chemical space between stars in the Sagittarius dwarf and the bulge stars. The strong HV peaks off-plane are still to be explained properly, and could be different in nature.Comment: 13 pages, 10 figures, published in ApJ. Updated to match the final ApJ published version. Minor revisions to the text and Figure

Non-Abelian Discrete Symmetries and Neutrino Masses: Two Examples

Two recent examples of non-Abelian discrete symmetries (S_3 and A_4) in understanding neutrino masses and mixing are discussed.Comment: 16 pages, no figure, invited contribution to NJP focus issue on neutrino

First DNA barcode reference library for the identification of South American freshwater fish from the lower Paraná river

Valid fish species identification is essential for biodiversity conservation and fisheries management. Here, we provide a sequence reference library based on mitochondrial cytochrome c oxidase subunit I for a valid identification of 79 freshwater fish species from the Lower Paraná River. Neighbour-joining analysis based on K2P genetic distances formed non-overlapping clusters for almost all species with a ≥99% bootstrap support each. Identification was successful for 97.8% of species as the minimum genetic distance to the nearest neighbour exceeded the maximum intraspecific distance in all these cases. A barcoding gap of 2.5% was apparent for the whole data set with the exception of four cases. Withinspecies distances ranged from 0.00% to 7.59%, while interspecific distances varied between 4.06% and 19.98%, without considering Odontesthes species with a minimum genetic distance of 0%. Sequence library validation was performed by applying BOLDs BIN analysis tool, Poisson Tree Processes model and Automatic Barcode Gap Discovery, along with a reliable taxonomic assignment by experts. Exhaustive revision of vouchers was performed when a conflicting assignment was detected after sequence analysis and BIN discordance evaluation. Thus, the sequence library presented here can be confidently used as a benchmark for identification of half of the fish species recorded for the Lower Paraná River.Instituto de Limnología "Dr. Raul A. Ringuelet

Multiple populations in Omega Centauri: a cluster analysis of spectroscopic data

Omega Cen is composed of several stellar populations. Their history might allow us to reconstruct the evolution of this complex object. We performed a statistical cluster analysis on the large data set provided by Johnson and Pilachowski (2010). Stars in Omega Cen divide into three main groups. The metal-poor group includes about a third of the total. It shows a moderate O-Na anticorrelation, and similarly to other clusters, the O-poor second generation stars are more centrally concentrated than the O-rich first generation ones. This whole population is La-poor, with a pattern of abundances for n-capture elements which is very close to a scaled r-process one. The metal-intermediate group includes the majority of the cluster stars. This is a much more complex population, with an internal spread in the abundances of most elements. It shows an extreme O-Na anticorrelation, with a very numerous population of extremely O-poor and He-rich second generation stars. This second generation is very centrally concentrated. This whole population is La-rich, with a pattern of the abundances of n-capture elements that shows a strong contribution by the s-process. The spread in metallicity within this metal-intermediate population is not very large, and we might attribute it either to non uniformities of an originally very extended star forming region, or to some ability to retain a fraction of the ejecta of the core collapse SNe that exploded first, or both. As previously noticed, the metal-rich group has an Na-O correlation, rather than anticorrelation. There is evidence for the contribution of both massive stars ending their life as core-collapse SNe, and intermediate/low mass stars, producing the s-capture elements. Kinematics of this population suggests that it formed within the cluster rather than being accreted.Comment: Accepted for publication in Astronomy and Astrophysic

Multiple populations in globular clusters. Lessons learned from the Milky Way globular clusters

Recent progress in studies of globular clusters has shown that they are not simple stellar populations, being rather made of multiple generations. Evidence stems both from photometry and spectroscopy. A new paradigm is then arising for the formation of massive star clusters, which includes several episodes of star formation. While this provides an explanation for several features of globular clusters, including the second parameter problem, it also opens new perspectives about the relation between globular clusters and the halo of our Galaxy, and by extension of all populations with a high specific frequency of globular clusters, such as, e.g., giant elliptical galaxies. We review progress in this area, focusing on the most recent studies. Several points remain to be properly understood, in particular those concerning the nature of the polluters producing the abundance pattern in the clusters and the typical timescale, the range of cluster masses where this phenomenon is active, and the relation between globular clusters and other satellites of our Galaxy.Comment: In press (The Astronomy and Astrophysics Review

All-oral combination of oral vinorelbine and capecitabine as first-line chemotherapy in HER2-negative metastatic breast cancer: an International Phase II Trial

BACKGROUND: This multicentre, international phase II trial evaluated the efficacy and safety profile of a first-line combination of oral vinorelbine plus capecitabine for women with metastatic breast cancer (MBC). METHODS: Patients with measurable, HER2-negative disease received, as a first line in metastatic setting, 3-weekly cycles of oral vinorelbine 80 mg m(-2) (after a first cycle at 60) on day 1 and day 8, plus capecitabine 1000 mg m(-2) (750 if >or=65 years of age) twice daily, on days 1-14. Treatment was continued until progression or unacceptable toxicity. RESULTS: A total of 55 patients were enrolled and 54 were treated (median age: 58.5 years). Most (78%) had visceral involvement and 63% had received earlier (neo)adjuvant chemotherapy. The objective response rate (RECIST) in 49 evaluable patients was 51% (95% confidence interval (CI), 36-66), including complete response in 4%. The clinical benefit rate (response or stable disease for >or=6 months) was 63% (95% CI, 48-77). The median duration of response was 7.2 months (95% CI, 6.4-10.2). After a median follow-up of 41 months, median progression-free survival was 8.4 months (95% CI, 5.8-9.7) and median overall survival was 29.2 months (95% CI, 18.2-40.1). Treatment-related adverse events were manageable, the main grade 3-4 toxicity was neutropaenia (49%); two patients experienced febrile neutropaenia and three patients had a neutropaenic infection (including one septic death). A particularly low rate of alopaecia was observed. CONCLUSION: These results show that the all-oral combination of oral vinorelbine and capecitabine is an effective and well-tolerated first-line regimen for MB