Changes of the energetic profile in masters' swimmers over a season

The aim of this study was to track and compare the changes of performance and energetic profile of male and female masters swimmers during a season. Eleven female (age: 34.7±7.3-y) and fourteen male (age: 35.6±7.4-y) with 4.2±3.7-y and 3.9±1.6-y of experience in masters, respectively, performed an all-out 200 m freestyle to evaluate total energy expenditure (Etot), aerobic (Aer), anaerobic lactic (AnL) and alactic (AnAl) contributions. The oxygen uptake (VO2) was measured immediately after the 200 m trial and the VO2 reached during the trial was estimated through the backward extrapolation of the O2 recovery curve. Fingertip capillary blood samples were collected before the 200 m trial and 3, 5, and 7 minutes after its end. Significant differences were observed between male (TP1:177.50±30.96s; TP2:174.79±29.08s; TP3:171.21±22.38s) and female (TP1:205.18±24.47s; TP2: 197.45±20.97s; TP3: 193.45±18.12s) for 200 m freestyle performance at the three time periods (TPs). Male presented higher Etot in all TPs (TP1:230.40±48.40kJ; TP2:242.49±37.91kJ; TP3:257.94±46.32kJ) compared with that found for female swimmers (TP1:188.51±35.13kJ; TP2:193.18±20.98kJ; TP3:199.77±25.94kJ). Male presented higher AnL (TP1:33.42±6.82kJ; TP2:30.97±8.73kJ; TP3:30.66±8.27kJ) and AnAl (TP1:30.61±3.48kJ; TP2:30.61±3.48kJ; TP3:30.60±3.48kJ) than female (TP1:18.83±8.45kJ; TP2:14.98±4.17kJ; TP3:18.33±8.66kJ) and (TP1:24.32±2.22kJ; TP2:24.31±2.23kJ; TP3: 24.31±2.23kJ). Aerobic metabolism is the major contributor for Etot both in male (TP1:71.63±4.99%; TP2:74.05±5.03%; TP3:76.14±4.46%) and female swimmers (TP1:76.87±3.86%; TP2:79.40±3.63%; TP3:78.40±5.54%). The better performance obtained by male compared to female swimmers may be due to the different contributions of the energetic pathways. Aerobic metabolism was the major contributor to Etot in a 200 m race, in both genders. Partial aerobic contribution was higher in female, while partial anaerobic contribution was greater in male.info:eu-repo/semantics/publishedVersio

Water enema computed tomography (WE-CT) in the local staging of low colorectal neoplasms: comparison with transrectal ultrasound

BACKGROUND: To determine the accuracy of computed tomography performed with a water enema application (WE-CT) in the local staging of low colorectal neoplasms and to compare the results with those of transrectal ultrasonography (TRUS). METHODS: Forty patients with low colorectal tumors were evaluated prospectively by CT with the simultaneous administration of a lukewarm rectal enema (0.5-1.5 L). Thin slices (5 mm) and intravenous application of iodinated contrast media were routinely used. TRUS was performed in 18 patients. Tumor size, location, and staging according to the TNM classification of the UICC were registered. Tumors were classified as or 5 mm in diameter was seen (reading A); N+ if at least one peritumoral node > or = 5 mm or three peritumoral nodes < 5 mm were identified (reading B). RESULTS: For the tumor staging, WE-CT showed a sensitivity of 90%, a specificity of 73%, a positive predictive value (PPV) of 90%, a negative predictive value (NPV) of 73%, and an accuracy of 85%. For TRUS, the results were sensitivity of 73%, specificity of 29%, PPV of 62%, NPV of 40%, and an accuracy of 39%. Concerning nodal staging with WE-CT, results were superior when reading A was used: sensitivity = 84%, specificity = 83%, PPV = 73%, NPV = 91%, and accuracy = 84%. TRUS showed a sensitivity of 29%, specificity of 100%, PPV of 100%, NPV of 67%, and an accuracy of 71%. CONCLUSION: WE-CT is a reliable technique for the local staging of low colorectal tumors that can be superior to TRUS. For diagnosis of peritumoral metastatic lymph nodes on WE-CT, the 5-mm diameter cutoff value is the most appropriate size criterion

Are wearable heart rate measurements accurate to estimate aerobic energy cost during low-intensity resistance exercise?

The aim of the present study was to assess the accuracy of heart rate to estimate energy cost during eight resistance exercises performed at low intensities: half squat, 45° inclined leg press, leg extension, horizontal bench press, 45° inclined bench press, lat pull down, triceps extension and biceps curl. 56 males (27.5 ± 4.9 years, 1.78 ± 0.06 m height, 78.67 ± 10.7 kg body mass and 11.4 ± 4.1% estimated body fat) were randomly divided into four groups of 14 subjects each. Two exercises were randomly assigned to each group and subjects performed four bouts of 4-min constant-intensity at each assigned exercise: 12%, 16%, 20% and 24% 1-RM. Exercise and intensity order were random. Each subject performed no more than 2 bouts in the same testing session. A minimum recovery of 24h was kept between sessions. During testing VO2 was measured with Cosmed K4b2 and heart rate was measured with Polar V800 monitor. Energy cost was calculated from mean VO2 during the last 30-s of each bout by using the energy equivalent 1 ml O2 = 5 calorie. Linear regressions with heart rate as predictor and energy cost as dependent variable were build using mean data from all subjects. Robustness of the regression lines was given by the scatter around the regression line (Sy.x) and Bland-Altman plots confirmed the agreement between measured and estimated energy costs. Significance level was set at p≤0.05. The regressions between heart rate and energy cost in the eight exercises were significant (p<0.01) and robustness was: half squat (Sy.x = 0,48 kcal·min-1), 45° inclined leg press (Sy.x = 0,54 kcal·min-1), leg extension (Sy.x = 0,59 kcal·min-1), horizontal bench press (Sy.x = 0,47 kcal·min-1), 45° inclined bench press (Sy.x = 0,54 kcal·min-1), lat pull down (Sy.x = 0,28 kcal·min-1), triceps extension (Sy.x = 0,08 kcal·min-1) and biceps curl (Sy.x = 0,13 kcal·min-1). We conclude that during low-intensity resistance exercises it is possible to estimate aerobic energy cost by wearable heart rate monitors with errors below 10% in healthy young trained males.This research was supported by Norte Portugal Regional Operational Programme (NORTE 2020), through Portugal 2020 and the European Regional Development Fund, NanoSTIMA, NORTE-01-0145- FEDER-000016 to VMR. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.info:eu-repo/semantics/publishedVersio

Acute Hormonal Responses to Multi-Joint Resistance Exercises with Blood Flow Restriction

The purpose of this study was to investigate the acute effects of multi-joint resistance exercises (MJRE) with blood flow restriction on hormonal responses. Ten men participated in the study and underwent two experimental protocols in random order: four sets (30, 15, 15, and 15 reps, respectively) of MJRE (half squat and horizontal chest press) were performed with 20% of 1RM and a rest time between sets of 30 s, combined with intermittent blood flow restriction (LI + BFR protocol); and four sets (8, 8, 8, 20 reps, respectively) of the same MJRE performed with 75% of 1RM load (HI protocol), with a 90 s rest between the first three sets and 30 s between the third to the fourth set. Blood samples were collected before (PRE), immediately after (POST), and 15 min after the performance of MJRE (POST15). A time effect was observed for growth hormone (GH) and insulin-like-growth-factor-1-binding-protein-3 (IGFPB-3), but no protocol effects or interactions between protocol and times were observed (p > 0.05). There was no effect of either protocol or time (p > 0.05) on total testosterone, free testosterone, or cortisol concentrations. However, significant (p < 0.05) increases were observed in the GH serum concentrations of 2072.73% and 2278.5%, HI, and LI + BFR protocols, respectively, from the PRE to POST15 test. In addition, there was an increase of 15.30% and 13.29% in the IGFPB-3 concentrations (p < 0.05) from PRE to POST0 times for HI and LI + BFR protocols, respectively. Furthermore, there was a decrease of 6.17% and 11.54%, p = 0.00, between the times POST0 to POST15 in the IGFPB-3 for the HI and LI + BFR protocols, respectively. It is concluded that multi-joint resistance exercises combined with intermittent blood flow restriction seemed to promote acute hormonal responses in a manner similar to traditional exercise with high loads. Future studies may investigate whether chronic use of LI + BFR with MJRE may promote muscle hypertrophy

Urinary schistosomiasis in Guinea Bissau

Urogenital schistosomiasis due to Schistosoma (S.) haematobium is among the most prevalent parasitoses in sub-Saharan Africa. The pathology is characterized by serious and irreversible lesions in the urogenital tract induced by chronic infection with the parasite that can eventually lead to renal failure due to hydronephrosis and to squamous cell carcinoma of the bladder. Considering the frequency and severe morbidity observed already in young children, the purpose of this pilot study was to assess the prevalence and morbidity of S. haematobium infection in Guinea Bissau. A baseline survey was conducted during September 2011. A randomly selected sample of 90 children aged 6–15 years old was included in this study. Prevalence of S. haematobium infection was 20% (18/90). It was higher in older children (median age in years: 15.4 2.71 vs. 9.3 2.22; P < 0.001), a significant gender difference in prevalence and intensity was not found. The predominant symptom was haematuria (87.1%), this symptom being strongly associated with S. haematobium infection (P < 0.01). Anthropometric examination revealed that growth in infected boys was impaired as compared to non-infected boys (median height in cm: 123.3 21.07 vs. 134.71 15.1) (P < 0.05). To our knowledge this is the first epidemiologic report of S. haematobium infection in Guinea Bissau. Considering the high prevalence of S. haematobium infections in Guinea Bissau and the long-term risks, including renal failure and bladder cancer, our results indicate that this population should be targeted for follow-up and implementation of measures for treatment and control of schistosomiasis

Are Strength Indicators and Skin Temperature Affected by the Type of Warm-Up in Paralympic Powerlifting Athletes?

(1) Background: the present study aimed to evaluate the effect of different types of warm-ups on the strength and skin temperature of Paralympic powerlifting athletes. (2) Methods: the participants were 15 male Paralympic powerlifting athletes. The effects of three different types of warm-up (without warm-up (WW), traditional warm-up (TW), or stretching warm-up (SW)) were analyzed on static and dynamic strength tests as well as in the skin temperature, which was monitored by thermal imaging. (3) Results: no differences in the dynamic and static indicators of the force were shown in relation to the different types of warm-ups. No significant differences were found in relation to peak torque (p = 0.055, F = 4.560, η2p = 0.246 medium effect), and one-repetition maximum (p = 0.139, F = 3.191, η2p = 0.186, medium effect) between the different types of warm-ups. In the thermographic analysis, there was a significant difference only in the pectoral muscle clavicular portion between the TW (33.04 ± 0.71 °C) and the WW (32.51 ± 0.74 °C) (p = 0.038). The TW method also presented slightly higher values than the SW and WW in the pectoral muscles sternal portion and the deltoid anterior portion, but with p-value > 0.05. (4) Conclusions: the types of warm-ups studied do not seem to interfere with the performance of Paralympic Powerlifting athletes. However, the thermal images showed that traditional warm-up best meets the objectives expected for this preparation phase.post-print1305 K

Contacts in the last 90,000 years over the Strait of Gibraltar evidenced by genetic analysis of wild boar (Sus scrofa)

[EN] Contacts across the Strait of Gibraltar in the Pleistocene have been studied in different research papers, which have demonstrated that this apparent barrier has been permeable to human and fauna movements in both directions. Our study, based on the genetic analysis of wild boar (Sus scrofa), suggests that there has been contact between Africa and Europe through the Strait of Gibraltar in the Late Pleistocene (at least in the last 90,000 years), as shown by the partial analysis of mitochondrial DNA. Cytochrome b and the control region from North African wild boar indicate a close relationship with European wild boar, and even some specimens belong to a common haplotype in Europe. The analyses suggest the transformation of the wild boar phylogeography in North Africa by the emergence of a natural communication route in times when sea levels fell due to climatic changes, and possibly through human action, since contacts coincide with both the Last Glacial period and the increasing human dispersion via the strait.This study was supported by The Emirates Centre for Wildlife Propagation (Morocco). The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.Soria-Boix, C.; Donat-Torres, MP.; Urios, V. (2017). Contacts in the last 90,000 years over the Strait of Gibraltar evidenced by genetic analysis of wild boar (Sus scrofa). PLoS ONE. 12(7). doi:10.1371/journal.pone.0181929S12

Significance of glycolytic metabolism-related protein expression in colorectal cancer, lymph node and hepatic metastasis

Background: Colorectal cancer (CRC) is one of the most common malignancies and a leading cause of cancer death worldwide. Most cancer cells display high rates of glycolysis with production of lactic acid, which is then exported to the microenvironment by monocarboxylate transporters (MCTs). The main aim of this study was to evaluate the significance of MCT expression in a comprehensive series of primary CRC cases, lymph node and hepatic metastasis. Methods: Expressions of MCT1, MCT4, CD147 and GLUT1 were studied in human samples of CRC, lymph node and hepatic metastasis, by immunohistochemistry. Results: All proteins were overexpressed in primary CRC, lymph node and hepatic metastasis, when compared with non-neoplastic tissue, with exception of MCT1 in lymph node and hepatic metastasis. MCT1 and MCT4 expressions were associated with CD147 and GLUT1 in primary CRC. These markers were associated with clinical pathological features, reflecting the putative role of these metabolism-related proteins in the CRC setting. Conclusion: These findings provide additional evidence for the pivotal role of MCTs in CRC maintenance and progression, and support the use of MCTs as biomarkers and potential therapeutic targets in primary and metastatic CRC.This work was supported by the Fundação para a Ciência e a Tecnologia (FCT) grant ref. PTDC/SAU-FCF/104347/2008, under the scope of ‘Programa Operacional Temático Factores de Competitividade’ (COMPETE) of ‘Quadro Comunitário de Apoio III’ and co-financed by the Fundo Europeu De Desenvolvimento Regional (FEDER). Ricardo Amorim was recipient of the fellowship SFRH/BD/98002/2013, from Fundação para a Ciência e a Tecnologia (FCT Portugal).info:eu-repo/semantics/publishedVersio

Análise das emissões otoacústicas evocadas por produto de distorção em neonatos prematuros

Objetivos: verificar a incidência de alterações nas Emissões Otoacústicas por Produto de Distorção em neonatos prematuros e analisar a amplitude das respostas em função da idade gestacional nessa população.Métodos: trata-se de um estudo transversal observacional, que contou com análise dos resultados do exame de emissões otoacústicas evocadas por produto de distorção dos neonatos pré-termos, triados em um hospital público de Belo Horizonte, no período de agosto de 2010 a fevereiro de 2011. Os neonatos avaliados foram divididos em três grupos de acordo com a idade gestacional, sendo o primeiro grupo constituído por neonatos de 28-30 semanas, o segundo de 31-33 semanas e o terceiro grupo de 34-36 semanas. Este estudo foi aprovado pelo Comitê de Ética da UFG sob parecer número 0210.0.203.000-10.Resultados: dentre as crianças avaliadas 44 (93,62%) apresentaram Emissões Otoacústicas Por Produto de Distorção (EOAPD) presentes e apenas três crianças (6,38%) apresentaram EOAPD ausentes. Com relação à análise das amplitudes das EOAs e suas comparações entre os grupos estudados, não foi encontrada diferença estatisticamente significante entre os grupos gestacionais, entretanto observou-se valores menores de p entre os grupos gestacionais nas frequências altas – 5KHz e 6KHz.Conclusão: observou-se que a prematuridade em si não constitui um fator que influencia no resultado de EOAPD em neonatos prematuros