64 research outputs found
TRY plant trait database - enhanced coverage and open access
Plant traits-the morphological, anatomical, physiological, biochemical and phenological characteristics of plants-determine how plants respond to environmental factors, affect other trophic levels, and influence ecosystem properties and their benefits and detriments to people. Plant trait data thus represent the basis for a vast area of research spanning from evolutionary biology, community and functional ecology, to biodiversity conservation, ecosystem and landscape management, restoration, biogeography and earth system modelling. Since its foundation in 2007, the TRY database of plant traits has grown continuously. It now provides unprecedented data coverage under an open access data policy and is the main plant trait database used by the research community worldwide. Increasingly, the TRY database also supports new frontiers of trait-based plant research, including the identification of data gaps and the subsequent mobilization or measurement of new data. To support this development, in this article we evaluate the extent of the trait data compiled in TRY and analyse emerging patterns of data coverage and representativeness. Best species coverage is achieved for categorical traits-almost complete coverage for 'plant growth form'. However, most traits relevant for ecology and vegetation modelling are characterized by continuous intraspecific variation and trait-environmental relationships. These traits have to be measured on individual plants in their respective environment. Despite unprecedented data coverage, we observe a humbling lack of completeness and representativeness of these continuous traits in many aspects. We, therefore, conclude that reducing data gaps and biases in the TRY database remains a key challenge and requires a coordinated approach to data mobilization and trait measurements. This can only be achieved in collaboration with other initiatives
Quantitative RT-PCR analysis of differentially expressed genes in Quercus suber in response to Phytophthora cinnamomi infection
cDNA-AFLP methodology was used to gain insight into gene fragments differentially present in the mRNA profiles of Quercus suber roots infected with zoospores of Phytophthora cinnamomi at different post challenge time points. Fifty-three transcript-derived fragments (TDFs) were identified and sequenced. Six candidate genes were selected based on their expression patterns and homology to genes known to play a role in defence. They encode a cinnamyl alcohol dehydrogenase2 (QsCAD2), a protein disulphide isomerase (QsPDI), a CC-NBS-LRR resistance protein (QsRPc), a thaumatin-like protein (QsTLP), a chitinase (QsCHI) and a 1,3-β-glucanase (QsGlu). Evaluation of the expression of these genes by quantitative polymerase chain reaction (qPCR) revealed that transcript levels of QsRPc, QsCHI, QsCAD2 and QsPDI increased during the first 24 h post-inoculation, while those of thaumatin-like protein decreased. No differential expression was observed for 1,3-β-glucanase (QsGlu).Four candidate reference genes, polymerase II (QsRPII), eukaryotic translation initiation factor 5A (QsEIF-5A), β-tubulin (QsTUB) and a medium subunit family protein of clathrin adaptor complexes (QsCACs) were assessed to determine the most stable internal references for qRT-PCR normalization in the Phytophthora-Q. suber pathosystem in root tissues. Those found to be more stable, QsRPII and QsCACs, were used as internal reference in the present work.Knowledge on the Quercus defence mechanisms against biotic stress is scarce. This study provides an insight into the gene profiling of a few important genes of Q. suber in response to P. cinnamomi infection contributing to the knowledge of the molecular interactions involving Quercus and root pathogens that can be useful in the future to understand the mechanisms underlying oak resistance to soil-borne oomycetes.Peer Reviewe
Social representations of needlestick injuries
OBJECTIVES: understand the Social Representations about needlestick injuries elaborated by Nursing Technicians and analyze how these representations influence their conducts. METHOD: the data, obtained by interviews, were processed using ALCESTE software and their analysis was based on Serge Moscovici's Social Representations Theory. RESULTS: it was evidenced that, after the accident, these professionals take care of the affected area. Then, they report the accident, motivated by the fear of catching HIV and hepatitis. The different feelings experienced are due to this fear and the way they were forwarded by the institution, reflecting in the cause they attribute to their accident. CONCLUSIONS: it was verified that knowledge about the accident as a whole is very incipient in this professional group, demanding continuing education and greater emphasis on this subject in professional training. It is expected that this study draws public authorities and health institutions' attention to the problem and that it modifies Nursing Technicians' Social Representations about percutaneous exposure.OBJETIVOS: Comprender las Representaciones Sociales de los accidentes con materiales corto-punzantes, elaboradas por Técnicos de Enfermería, así como analizar de que modo esas representaciones influencian las conductas de eses profesionales. MÉTODO: Los datos, logrados por medio de entrevistas, fueron procesados por el software ALCESTE y el análisis de los mismos fue basado en la Teoría de las Representaciones Sociales de Serge Moscovici. RESULTADOS: se evidenció que, después de accidentarse, los Técnicos de Enfermería cuidan del área afectada. Enseguida, notifican el accidente, motivados por el miedo de que contraigan HIV y hepatitis. Los diversos sentimientos vividos son consecuentes de ese miedo y de la manera como fueron encaminados por la institución, reflejando en la causa que ellos atribuyen a su accidente. CONCLUSIONES: se constató que hay un conocimiento bastante incipiente por parte de esa clase profesional sobre el accidente como un todo, habiendo necesidad de una educación continuada y mayor énfasis en cuanto a ese asunto durante la formación profesional. Se espera que ese estudio llame la atención de los órganos gubernamentales e instituciones de salud sobre el problema y modifique las Representaciones Sociales de la exposición percutánea de los Técnicos de Enfermería.OBJETIVOS: compreender as representações sociais dos acidentes com materiais perfurocortantes, elaboradas por técnicos de enfermagem, bem como analisar de que modo essas representações influenciam as condutas desses profissionais. MÉTODO: os dados, obtidos por meio de entrevistas, foram processados pelo software Alceste e a análise dos mesmos foi baseada na Teoria das Representações Sociais de Serge Moscovici. RESULTADOS: evidenciou-se que, após se acidentarem, os técnicos de enfermagem cuidam da área afetada. Em seguida, notificam o acidente, motivados pelo medo de contraírem HIV e hepatite. Os diversos sentimentos vivenciados são decorrentes desse medo e da forma como foram encaminhados pela instituição, refletindo na causa que eles atribuem ao seu acidente. CONCLUSÕES: constatou-se que há conhecimento bastante incipiente por parte dessa classe profissional sobre o acidente, como um todo, havendo necessidade de educação continuada e maior ênfase quanto a esse assunto, durante a formação profissional. Espera-se que este estudo chame a atenção dos órgãos governamentais e instituições de saúde sobre o problema e modifique as representações sociais da exposição percutânea dos técnicos de enfermagem
Cultural singularities: indigenous elderly access to Public Health Service
OBJECTIVEDescribing how Kaingang seniors and their primary caregivers experience access to public health services.METHODA qualitative study guided by ethnography, conducted with 28 elderly and 19 caregivers. Data were collected between November 2010 and February 2013 through interviews and participative observation analyzed by ethnography.RESULTSThe study revealed the benefits and difficulties of the elderly access to health services, the facility to obtain health care resources such as appointments, medications and routine procedures, and the difficulties such as special assistance service problems and delays in the dispatching process between reference services.CONCLUSIONThe importance of knowing and understanding the cultural specificities of the group in order to offer greater opportunities for the elderly access to health services was reinforced
Cuidadores informais de idosos em pós-operatório de cirurgia de fêmur proximal: prevenção de novas quedas
The objectives of this study were to investigate the sociodemographic characteristics of informal caregivers of elderly persons who had undergone surgery for hip fractures caused by a fall, explore the level of caregiver's knowledge regarding fall prevention, and assess the relationship between this knowledge and the use of preventative measures in practice. This investigation consists of a cross-sectional study using nonprobability sampling methods conducted over a period of 12 months and involving 89 caregivers. The majority of caregivers were female (76.4%) and sons or daughters of the patients (64%). Environmental modification was the predominant preventative measure used by caregivers (88.2%). 58.1% of caregivers believed it was possible to prevent falls in the elderly and there was a significant association (p = 0,002) between believing it was possible to prevent falls and carrying out modifications in the home and/or to the daily routine of the older person. Informal caregivers with wide or partial knowledge of fall prevention put preventative measures into practice. These findings demonstrate that the number of falls among older persons could be significantly reduced if health care programmes widened their actions to include the guiding principles of the WHO falls prevention model.Os objetivos foram caracterizar sociodemograficamente os cuidadores informais de idosos vítimas de queda seguida por fratura de fêmur proximal e verificar o conhecimento mínimo que possuíam acerca da prevenção de novas quedas, assim como caracterizar a relação entre esse conhecimento e o emprego de medidas preventivas. Trata-se de estudo transversal, com amostragem intencional, realizado em 12 meses, incluindo 89 cuidadores. Predominaram cuidadores do sexo feminino (76,4%) e filhas(os) (64%). A modificação ambiental foi a medida preventiva predominante apontada por eles (88,2%). Houve associação significativa (p = 0,002), entre os 58,1% dos cuidadores que achavam ser possível prevenir quedas e os relatos sobre mudanças na casa e/ou rotina do idoso. Observou-se que cuidadores informais que apresentavam conhecimento sobre prevenção de quedas em idosos, mesmo que incompletos, empregavam medidas de prevenção para novos eventos. Esses achados sinalizam que o número de quedas entre idosos pode ser reduzido significantemente se os programas de atenção à saúde ampliarem suas ações apoiando-as no modelo de prevenção de quedas da Organização Mundial de Saúde.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Estadual Paulista (Unesp), Departamento de Enfermagem, Faculdade de Medicina de Botucatu (FMB), Distrito de Rubião Júnior snº, Centro. 18618-970, Botucatu SP BrasilUniversidade Estadual Paulista (Unesp), Departamento de Cirurgia e Ortopedia, FBM, Unesp.Universidade Estadual Paulista (Unesp), Departamento de Enfermagem, Faculdade de Medicina de Botucatu (FMB), Distrito de Rubião Júnior snº, Centro. 18618-970, Botucatu SP BrasilUniversidade Estadual Paulista (Unesp), Departamento de Cirurgia e Ortopedia, FBM, Unesp
Quality of life of caregivers for patients of cerebrovascular accidents: association of (socio-demographic) characteristics and burden
OBJECTIVE Investigating the association between quality of life with socio-demographic characteristics and the burden of caregivers for individuals with cerebrovascular accident sequelae. METHOD A descriptive, cross-sectional study with a sample composed of 136 caregivers. For data collection, a semi-structured questionnaire, the Barthel, Burden Interview and Short-Form-36 scales were used. Correlation analysis, t-Student test and F-test were used for the analysis in order to compare averages. RESULTS Significant averages in quality of life were demonstrated in association with female caregivers and those over 60 years in the field 'functional capacity,' and in the domains of 'mental health' and 'vitality' for those with higher income. Regarding burden association, the highlighted areas were 'functional capacity,' 'physical aspects,' 'emotional aspects' and 'pain.' CONCLUSION The creation of public policies and social support to effectively reduce the burden on caregivers is a necessity
Acidente ocupacional por material perfurocortante entre profissionais de saúde de um Centro Cirúrgico
O acidente ocupacional por material perfurocortante constitui uma preocupação para instituições e trabalhadores de saúde, devido à elevada frequência de procedimentos invasivos, e a dinâmica do trabalho. Objetivou-se identificar a incidência dos acidentes, dos materiais envolvidos, dos fatores contribuintes e das condutas tomadas pós-acidente. Participaram de um estudo transversal 127 funcionários do centro cirúrgico. Registraram-se 23,6% (30/127) acidentes com envolvimento de agulha (73,3%), lâmina de bisturi (6,7%) e eletrocautério (6,7%). Os fatores contribuintes para o acidente foram: falta de atenção (36,7%), más condições de trabalho (20,0%), descuido (13,3%), pressa (10%) e acaso/azar (6,7%). Somente 15,4% dos acidentes foram registrados. A subnotificação deveu-se à irrelevância do acidente, desconhecimento do protocolo de rotina, displicência e sobrecarga de trabalho. Os resultados alertam para a importância de se implementar estratégias para adoção/revisão de protocolos pós-acidentes, visando a redução dos acidentes e de sua subnotificação.El accidente laboral por elementos corto-punzantes constituye una preocupación para instituciones y trabajadores de la salud, debido a la elevada frecuencia con la que se realizan procedimientos invasivos y por la dinámica del trabajo. Se objetivó identificar la incidencia de los accidentes, los materiales involucrados, los factores contribuyentes y las conductas tomadas con posterioridad al accidente. Participaron de un estudio transversal 127 trabajadores de un centro quirúrgico. Sobre un porcentaje de accidentados del 23,6% (30/127), el 73,3% sufrió accidentes involucrando agujas, 6,7% se accidentó con hojas de bisturí y 6,7% se accidentó con electrocauterizador. Los factores que contribuyeron a los accidentes fueron: falta de atención (36,7%), malas condiciones de trabajo (20,0%), descuido (13,3%), prisa (10,0%) y casualidad / azar (6,7%). Sólo el 15,4% de los accidentes fueron registrados. La falta de notificación se debió a la irrelevancia del accidente, desconocimiento del protocolo de rutina, displicencia y sobrecarga de trabajo. Los resultados alertan sobre la importancia de implementar estrategias para adopción / revisión de protocolos post accidentes, apuntando a la reducción de la cantidad de accidentes y a evitar la ausencia de registro de los mismos.Occupational accidents involving piercing-cutting material are a concern for institutions and health workers due to the high frequency of invasive procedures and the dynamics involved in their practice. The objective of the present study was to identify the incidence of accidents, devices involved, contributing factors and post-accident behaviors. A cross-sectional study was conducted among 127 healthcare workers from the operating center. Of the total number of accidents reported, 23.6% (30/127) involved piercing-cutting materials, including needles (73.3%), scalpels (6.7%) and electrocautery (6.7%). The factors contributing to the accident were: lack of attention (36.7%), poor working conditions (20.0%), neglect (13.3%), rushing (10%) and accident/chance (6.7%). Only 15.4% of the accidents were recorded. Underreporting was due to: irrelevance of the accident, unaware of the protocol of routine, negligence and work overload. Results show the importance of implementing strategies for adopting/reviewing post-accident protocols aiming at reducing accidents and their underreporting
Comparative anatomy of calyx and foliar glands of Banisteriopsis C. B. Rob. (Malpighiaceae)
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