Role of non-coding RNAs in non-aging-related neurological disorders

CNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOCAPES - COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIORFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOProtein coding sequences represent only 2% of the human genome. Recent advances have demonstrated that a significant portion of the genome is actively transcribed as non-coding RNA molecules. These non-coding RNAs are emerging as key players in the regula518CNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOCAPES - COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIORFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOCAPES - COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIORFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOsem informaçãosem informação2016/22447-52011/506802013/07559-

Distinct incubation for homologous in vitro spermatozoa binding on swine oocytes subjected to different storage conditions

AbstractThe sperm in vitro binding assay in homologous oocytes can be used to estimate the boar fertility potential, but its usefulness may be limited by laboratorial structure and oocytes availability. This study aimed at determining the effect of distinct methods of oocytes conditioning and incubation media for the in vitro penetration (IVP) test. Oocytes used in the IVP test were: fresh and conditioned in PBS (T1); cooled and conditioned in PBS at 5°C for 48h (T2); or stored in ovaries frozen at −20°C (T3). For each treatment, two incubation media were tested at 39°C for 6h: modified TRIS buffer medium (mTBM); or Beltsville Thawing Solution (BTS) extender. The responses of interest were: IVP and polyspermy rates; and the number of penetrating spermatozoa per oocyte. All responses observed with incubation in BTS were inferior to those observed with incubation in mTBM (P<0.0001). When incubation was done in mTBM, none of the responses differed across treatments (P>0.05). However, when incubation was in BTS, all the three responses were superior for T1 than for T2 and T3 (P<0.05). Thus, the IVP test may be conducted with ovaries either cooled or recovered from frozen ovaries with results similar to those observed with fresh oocytes, if incubation is done in mTBM

Theoretical and experimental study on electron interactions with chlorobenzene: Shape resonances and differential cross sections

9 págs.; 6 figs.; 1 tab.In this work, we report theoretical and experimental cross sections for elastic scattering of electrons by chlorobenzene (ClB). The theoretical integral and differential cross sections (DCSs) were obtained with the Schwinger multichannel method implemented with pseudopotentials (SMCPP) and the independent atom method with screening corrected additivity rule (IAM-SCAR). The calculations with the SMCPP method were done in the static-exchange (SE) approximation, for energies above 12 eV, and in the static-exchange plus polarization approximation, for energies up to 12 eV. The calculations with the IAM-SCAR method covered energies up to 500 eV. The experimental differential cross sections were obtained in the high resolution electron energy loss spectrometer VG-SEELS 400, in Lisbon, for electron energies from 8.0 eV to 50 eV and angular range from 7 to 110. From the present theoretical integral cross section (ICS) we discuss the low-energy shape-resonances present in chlorobenzene and compare our computed resonance spectra with available electron transmission spectroscopy data present in the literature. Since there is no other work in the literature reporting differential cross sections for this molecule, we compare our theoretical and experimental DCSs with experimental data available for the parent molecule benzene. Published by AIP Publishing.A.S.B., M.T.N.V., S.d’A.S., and M.H.F.B. acknowledge the Brazilian Agency Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES), under CAPES/FCT Programme (Process No. 23038.002465/2014-87). M.T.N.V., S.d’A.S., and M.H.F.B. acknowledge support from the Brazilian Agency Conselho Nacional de Desenvolvimento Científico e Tecnológico. M.H.F.B. acknowledges support from Finep (under project CT-Infra), and M.T.N.V. from São Paulo Research Foundation (FAPESP). A.S.B., S.d’A.S., and M.H.F.B. acknowledge computational support from Professor Carlos M. de Carvalho at LFTC-DFis-UFPR and at LCPADUFPR and from CENAPAD-SP. F.F.S. acknowledges the Portuguese National Funding Agency FCT through researcher Contract No. IF-FCT IF/00380/2014 and together with P.LV. the research Grant No. UID/FIS/00068/2013. F.B. and G.G. acknowledge partial financial support from the Spanish Ministry MINECO (Project No. FIS2012-31230).Peer Reviewe

RNA sequencing reveals region-specific molecular mechanisms associated with epileptogenesis in a model of classical hippocampal sclerosis

We report here the first complete transcriptome analysis of the dorsal (dDG) and ventral dentate gyrus (vDG) of a rat epilepsy model presenting a hippocampal lesion with a strict resemblance to classical hippocampal sclerosis (HS). We collected the dDG6CAPES - COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIORFAPESP - FUNDAÇÃO DE AMPARO À PESQUISA DO ESTADO DE SÃO PAULOCNPQ - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOsem informaçãosem informaçãosem informaçã

Anatomy of BioJS, an open source community for the life sciences

BioJS is an open source software project that develops visualization tools for different types of biological data. Here we report on the factors that influenced the growth of the BioJS user and developer community, and outline our strategy for building on this growth. The lessons we have learned on BioJS may also be relevant to other open source software projects

Ânus anterior: está ou não associado com a constipação crônica na criança?

OBJECTIVE: To study the association between the anterior anus, measured through anal position index, and the presence of longstanding chronic constipation in children.MATERIALS AND METHODS: 195 pediatric patients of the outpatient unit at HCPA were analyzed (ages: from 10 days to 9 years and 10 months; sex: 94 girls and 101boys). Children were considered constipated when presenting a period of more than 3 days without bowel movements and large and hard, or pelletlike, stools. Anterior anus was identified by anal indexes lower than the average minus the standard deviation of the control group of children without constipation; that is &lt; 0.40 for boys, and &lt; 0.24 for girls.RESULTS: Chronic constipation was diagnosed in 58 of the 195 children (29.7%; 28.7% of boys, and 30.9% of girls). There was no significant difference in relation tosex, constipation, or regarding the presence of anterior anus. Fifty-eight children (39.4%) with constipation had anterior anus, whereas 60.6% of children without constipation had anterior anus. There was no significant correlation between anterior position of anus and chronic constipation.CONCLUSION: There was no correlation between chronic constipation and anterior anus among the analyzed children.OBJETIVO: Estudar a associação entre o deslocamento ventral do ânus, através da medida do índice ânus-genital, e a presença de constipação crônica em crianças.MATERIAL E MÉTODOS: Foram estudadas 195 crianças atendidas no ambulatório de gastroenterologia pediátrica do HCPA, com idades entre 10 dias e 9 anos e 10 meses, 94 meninas e 101 meninos. Foram consideradas constipadas as crianças que não evacuavam há mais de 3 dias e que apresentavam fezes disquézicas ou cibalosas. Ânus anteriorizado fois definido como IAG menor do que um desvio-padrãodaquele obtido da média das crianças controles não constipadas (IAG &lt; 0,40 em meninos e &lt; 0,24 em meninas).RESULTADOS: Foram identificadas 58/195 crianças (29,7%) com constipação crônica (28,7% em meninos e 30,9% em meninas). Não houve diferença estatisticamente significativa em relação ao sexo (30,9%) ou quanto à presença do ânus anterior. Entre as 58 crianças constipadas, 39,4% apresentavam ânus anteriorizado, enquanto que dentre as não constipadas 60,6% o apresentavam. Não houve correlação estatisticamente significativa entre a presença de ânus anteriorizado e constipação, ou entre constipação e as medidas do IAG.CONCLUSÃO: Não houve relação entre constipação e a posição anteriorizada do ânus nas crianças estudada