Tag shedding estimation in Palinurus elephas (Fabricius, 1787)

In this paper a numerical method for tag shedding calculation from double tagging data was used to estimate the tag loss rate in Palinurus elephas from capture-mark-recapture experiments. Four consecutive experimental surveys during years 1999-2002 inside the Columbretes islands marine reserve (CIMR) were carried out and the captured spiny lobsters were dorso-laterally double-tagged with T-bar anchor tags on both sides between the first and second abdominal segments. The recaptures during the following years (2000-2008) were recorded and the evolution of the proportion of single- and double-tagged specimens was analysed. The immediate tag loss produced after tagging could not be accurately modelled, but in view of the results is believed to be low. The probability of tag loss per year was estimated in 6% (sexes combined). The factors causing this tag loss and the repercussion of this rates of shedding on further studies are examine

Double tagging experiments and tag loss in Palinurus elephas

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Plant Metabolites in Plant Defense Against Pathogens

Medicinal plants are widely used worldwide to treat various diseases. Its widespread use is due in part to the cultural acceptance of traditional medicine in different regions of the world, as well as its effectiveness in treating various diseases. Many of its active substances or secondary metabolites are formed to a response of various situations that generate stress in their habitat, such as sudden changes in environmental temperature, humidity, rain, drought, and infections by phytopathogens (fungi, bacteria, viruses, nematodes, protozoa). The production of these secondary metabolites is a mechanism of defense of plants. In this context, the objective of this chapter is to study the secondary metabolites of medicinal plants that could have a promising application in the control of different phytopathogens in crops of agricultural and economic interest

Will we see you again? Population evaluation of Palinurus elephas inside a marine reserve from Capture-Mark-Recapture data

To achieve an effective management of the marine exploited populations it is necessary to understand their dynamics and the processes driving short- and long-term changes. Key population parameters such as survival and movement can be estimated by means of capture-mark-recapture studies, based on the capture, tagging and re-encounter of the individuals at later times. The Cormack-Jolly-Seber model, which assesses the apparent survival i and the recapture rate pi of a population conditional on first capture, was applied to European spiny lobster Palinurus elephas data from the Columbretes islands marine reserve to evaluate the evolution of this population after closure to fishing over. Particularly strong winter conditions were reported for some years during the period of study whcih were reflected as an increase in the estimate of the encounter probability and a decrease in their survival rate. P. elephas showed a relatively high plasticity against short-term environmental effects, with the apparent survival quickly returning to pre-storm levels. Also a slight increase in survival was identified for this protected population over time. These preliminary results are discussed in terms of the factors affecting the dynamics of this population and the implications for the management of this exploited stoc

Clinical Relevance of Medicinal Plants and Foods of Vegetal Origin on the Activity of Cytochrome P450

Drug metabolism is a pharmacokinetic process whose main objective is to modify the chemical structure of drugs to easily excretable compounds. This process is carried out through phase I and phase II reactions. The enzymes of cytochrome P450 (CYP450) participate in phase I reactions, and their activity can be inhibited or induced by xenobiotics. The aim of this chapter is to study the clinical relevance of the induction and inhibition of CYP450, by describing the effect that some bioactive compounds present in medicinal plants or foods can modify, either increasing or decreasing the activity of CYP450 enzymes and with it modify the bioavailability and depuration of drugs. Examples will be described on the interaction of medicinal plants and foods of vegetal origin that when combined with some drugs can generate toxicity or therapeutic failure; this will allow gathering relevant information on the adequate pharmacological management in different clinical situations

Assessing gastro-intestinal related quality of life in cystic fibrosis: Validation of PedsQL GI in children and their parents

Background: Most patients with cystic fibrosis (CF) suffer from pancreatic insufficiency, leading to fat malabsorption, malnutrition and abdominal discomfort. Until recently, no specific tool was available for assessing gastro-intestinal related quality of life (GI QOL) in patients with CF. As the Horizon2020 project MyCyFAPP aims to improve GI QOL by using a newly designed mobile application, a sensitive and reliable outcome measure was needed. We aimed to study the applicability of the existing child-specific Pediatric Quality of Life Inventory, Gastrointestinal Symptoms Scales and Module (PedsQL GI) in children with CF. Methods: A multicenter, prospective observational study was performed in 6 European centers to validate the PedsQL GI in children with CF during 3 months. Results: In total, 248 children and their parents were included. Within-patient variability of PedsQL GI was low (24.11), and there was reasonable agreement between children and parents (ICC 0.681). Nine of 14 subscales were informative (no ceiling effect). The PedsQL GI and the median scores for 4 subscales were significantly lower in patients compared to healthy controls. Positive associations were found between PedsQL GI and age (OR = 1.044, p = 0.004) and between PedsQL GI and BMI z-score (OR = 1.127, p = 0.036). PedsQL GI correlated with most CFQ-R subscales (r 0.268 to 0.623) and with a Visual Analogue Scale (r = 0.20). Conclusions: PedsQL GI is a valid and applicable instrument to assess GI QOL in children with CF. Future research efforts should examine the responsiveness of the CF PedsQL GI to change in the context of clinical interventions and trials

Producción de biodiésel a partir de microalgas: avances y perspectivas biotecnológicas

Las microalgas son una alternativa para la obtención de biodiésel por su alto rendimiento de lípidos y su perfil de ácidos grasos. Objetivos. Hacer una revisión sobre los avances y perspectivas actuales de la producción de biodiésel a partir de microalgas. Métodos. Se realizó una búsqueda actualizada de los trabajos de investigación relacionados con la producción de biodiésel a partir de microalgas, con especial énfasis en la biosíntesis de ácidos grasos y triglicéridos, la producción de biomasa, las técnicas de extracción, los procesos biotecnológicos implementados en sistemas de cultivo, la transesterificación y los sistemas de doble propósito. Resultados. Las microalgas tienen rendimientos altos de producción de lípidos (59 m3 ha-1 año-1), por lo que representa una alternativa para la obtención de biodiésel; sin embargo, el costo de producción y recuperación de biomasa sigue siendo elevado ($5.8 USD Kg-1), aunado a los altos requerimientos energéticos (33 MJ Kg-1). Actualmente no existen técnicas industriales factibles para la extracción de lípidos y se están probando los métodos por fluidos supercríticos, campo eléctrico de pulso, microondas y ultrasonicación. La biotecnología ha propuesto un novedoso sistema biológico mediante el uso de lipasas recuperadas de hongos filamentosos para el proceso de transesterificación, los cuales ya son comerciales, y ha logrado rendimientos de biocatálisis mayores al 90%.Los sistema de doble propósito pueden ser optimizados utilizando un diseño modular que establezca los procesos y operaciones unitarias bien definidas. Conclusiones. El uso de microalgas para la obtención de biodiésel representa una técnica viable gracias a su alto contenido lipídico y a su perfil de ácidos grasos, aunque hace falta el desarrollo de tecnologías que disminuyan el costo de producción. El uso de sistemas de doble propósito se vislumbra como una buena opción para reducir estos precios, al mismo tiempo que se reusan aguas residuales

Detection of human bocavirus and human metapneumovirus by real-time PCR from patients with respiratory symptoms in Southern Brazil

The introduction of newer molecular methods has led to the discovery of new respiratory viruses, such as human metapneumovirus (hMPV) and human bocavirus (hBoV), in respiratory tract specimens. We have studied the occurrence of hMPV and hBoV in the Porto Alegre (PA) metropolitan area, one of the southernmost cities of Brazil, evaluating children with suspected lower respiratory tract infection from May 2007-June 2008. A real-time polymerase chain reaction method was used for amplification and detection of hMPV and hBoV and to evaluate coinfections with respiratory syncytial virus (RSV), influenza A and B, parainfluenza 1, 2 and 3, human rhinovirus and human adenovirus. Of the 455 nasopharyngeal aspirates tested, hMPV was detected in 14.5% of samples and hBoV in 13.2%. A unique causative viral agent was identified in 46.2% samples and the coinfection rate was 43.7%. For hBoV, 98.3% of all positive samples were from patients with mixed infections. Similarly, 84.8% of all hMPV-positive results were also observed in mixed infections. Both hBoV and hMPV usually appeared with RSV. In summary, this is the first confirmation that hMPV and hBoV circulate in PA; this provides evidence of frequent involvement of both viruses in children with clinical signs of acute viral respiratory tract infection, although they mainly appeared as coinfection agents

The Root-Colonizing Endophyte Piriformospora indica Supports Nitrogen-Starved Arabidopsis thaliana Seedlings with Nitrogen Metabolites

16 Pág.The root-colonizing endophytic fungus Piriformospora indica promotes the root and shoot growth of its host plants. We show that the growth promotion of Arabidopsis thaliana leaves is abolished when the seedlings are grown on media with nitrogen (N) limitation. The fungus neither stimulated the total N content nor did it promote 15NO3- uptake from agar plates to the leaves of the host under N-sufficient or N-limiting conditions. However, when the roots were co-cultivated with 15N-labelled P. indica, more labels were detected in the leaves of N-starved host plants but not in plants supplied with sufficient N. Amino acid and primary metabolite profiles, as well as the expression analyses of N metabolite transporter genes suggest that the fungus alleviates the adaptation of its host from the N limitation condition. P. indica alters the expression of transporter genes, which participate in the relocation of NO3-, NH4+ and N metabolites from the roots to the leaves under N limitation. We propose that P. indica participates in the plant's metabolomic adaptation against N limitation by delivering reduced N metabolites to the host, thus alleviating metabolic N starvation responses and reprogramming the expression of N metabolism-related genes.This project was financially supported by the collaborative ICPS research project executed in the framework of the EIG CONCERT-Japan joint call on Food Crops and Biomass Production Technologies and the related national funding agencies: grants 01DR17007A and 01DR17007B from the Federal Ministry of Education and Research (BMBF), Germany, to R.O.; grant EIG_JC1JAPAN-045 from the Centre National de la Recherche Scientifique (CNRS), France, to A.K.; grant PCIN-2016–037 from the Ministry of Economy and Competitiveness (MINECO), Spain, to J.V.-C. and S.P.; and grant JPMJSC16C3 from the Japan Science and Technology Agency (JST) to H.S. This work was further supported by the Deutsche Forschungsgemeinschaft (DFG, German Research Foundation) CRC1127 ChemBioSys (project ID: 239748522) for R.O. This work benefited from the support of IJPB’s Plant Observatory technological platforms. The IJPB benefits from additional support of Saclay Plant Sciences-SPS (ANR-17-EUR-0007).Peer reviewe

Prevalence of sleep disorders in adults with down syndrome: a comparative study of subjective, actigraphic and polygraphic findings

STUDY OBJECTIVES:Sleep problems are often undetected in adults with Down syndrome (DS). Our objective was to determine the prevalence of sleep disorders in adults with DS through self-reported and objective sleep measures. METHODS:We performed a community-based cross-sectional study of 54 adults with DS not referred for sleep disorders. Two polysomnography (PSG) sleep studies were performed. Sleep quality was evaluated using the Pittsburgh Sleep Quality Index (PSQI); daytime sleepiness was evaluated using the Epworth Sleepiness Scale (ESS) and the risk for the sleep apnea syndrome (OSA) was identified using the Berlin Questionnaire (BQ). Participants' sleep/wake pattern was assessed from sleep diaries and by wrist actigraphy. PSQI, ESS, and PSG measures were compared with 35 sex-, age-, and body mass index-matched patients in the control groups. RESULTS:In PSG measures, adults with DS showed lower sleep efficiency (69 ± 17.7 versus 81.6 ± 11; P < .001), less rapid eye movement sleep (9.4 ± 5.8 versus 19.4 ± 5.1; P < .001), a higher prevalence of OSA (78% versus 14%; P < .001), and a higher apnea-hypopnea index (23.5 ± 24.5 versus 3.8 ± 10.5; P < .001) than patients in the control group. In the DS group, the questionnaires (mean PSQI 3.7 ± 2.9; mean ESS 6.3 ± 4.5 and mean BQ 1 ± 0) did not reflect the sleep disturbances detected on the PSG. Actigraphy data recorded daytime sleep that was not self-reported (118.2 ± 104.2 minutes). CONCLUSIONS:Adults with DS show severe sleep disruption and a high prevalence of OSA, undetected by self-reported sleep measures. Actigraphy, PSG, and validated simplified devices for screening OSA should be routinely recommended for this population because treatment of sleep disorders can contribute to healthy aging