Nadzor bolesti riba u Srbiji

U Srbiji se vrši program nadzora virusnih i bakterijskih bolesti na osnovu Programa mera zdravstvene zaštite životinja, u skladu sa propisima Evropske Unije, i to: virusne hemoragične septikemije (VHS) i zarazna hematopoezne nekroze (IHN), zarazne nekroze pankreasa (IPN), prolećne viremije šarana (SVC) i renibakterioze (BKD). Cilj programa praćenja i nadzora bolesti riba je dobijanje i održavanje statusa slobode od bolesti, iskornjivanje ili sprečavanje širenja bolesti. Primarna ciljna populacija u programu nadzora su kalifornijska pastrmka i šaran. U Srbiji postoji nacionalno zakonodavstvo kao osnov za nadzora i kontrolu bolesti riba, kao i lista bolesti obaveznih za prijavljivanje. Pored nacionalnog zakonodavstva, prihvaćeni su i principi navedeni u Direktivi 2006/88/EC, koji se odnose na zahteve za kontrolu zdravlje riba u akvakulturi i njihovih proizvoda. Praćenje i nadzor virusnih bolesti vrše se na osnovu postupaka ispitivanja datih u Odluci Komisije 2001/183/EC, a za bakterijske bolesti, koriste se standardne dijagnostičke procedure. Klinički pregledi na ribnjacima vrše se dva puta godišnje i uzimaju se uzorci za virološka i bakteriološka ispitivanja, u svrhu dokumentovanja odsustva bolesti. Postupak ispitivanja, dat u Priručniku OIE za dijagnostiku bolesti riba, osnova je za ispitivanja. Uzorci svih kategorija riba iz 56 šaranskih i 52 pastrmska ribnjaka se godišnje ispituju na prisustvo bolesti. Za virusološka ispitivanja su korišćeni homogenati bubrega, slezine, jetre i škrga. Pulirani parenhimatozni organi i škrge su homogenizovani i centrifugirani na 2500 x g, 20 minuta. Za izolaciju, supernatanti su inokulirani na 24 sata stare kulture EPC i BF-2 ćelijskih linija. Inokulisane kulture su inkubirane na 15 - 20 °C, tokom 7 dana i svakodnevno su posmatrane na pojavu citopatogenog efekta. Identifikacija virusa je vršena PCR, ELISA testom i testom fluorescentnih antitela. Kao materijal za PCR je uziman homogenat organa i prva ili druga pasaža odgovarajuće ćelijske linije. PCR produkti su sekvencirani direktno, pomoću Big Dye Terminator v1.1 Cycle Sequencing Kit (Applied Biosystems, SAD) i ABI PRISM 3100-Avant Genetic Analyzer (Applied Biosystems). Dobijene sekvence su analizirane pomoću Sequencing Analysis Software 5.1 (Applied Biosystems). Na osnovu rezultata nadzora, Srbija se smatra slobodnom od VHS, IHN i KHV. Najveći problem predstavlja zarazna nekroza pankreasa. Renibakterioza je prisutna na određenom broju ribnjaka

Further evidence that inhibition of neuronal voltage-gated calcium channels contributes to the hypnotic effect of neurosteroid analogue, 3β-OH

We recently reported that a neurosteroid analogue with T-channel-blocking properties (3β,5β,17β)-3-hydroxyandrostane-17-carbonitrile (3β-OH), induced hypnosis in rat pups without triggering neuronal apoptosis. Furthermore, we found that the inhibition of the C

Karakterizacija prevlake 316L nanete postupkom plazma navarivanja

Parts of industrial machines and structures are often exposed to the action of aggressive environments, which in a short period of time can provoke the loss of their integrity. It is well known that for extending the service life against erosion and corrosion, protection of the exposed structure zone by coating is frequently used. Various application methods of protective coatings are applied, and the most common are welding and thermal spraying processes. The aim of this study was characterization of coatings made of stainless steel 316L, widely used in chemical and petrochemical industries. The coating was applied on a structural steel S235JR by plasma transferred arc using powder as a filler material. Due to a number of advantages, the plasma transferred arc (PTA) surfacing process has found significant usages in the field of surface protection. This paper presents results of hardness measurements in characteristic zones of the coating and the base material, as well as microstructural characterization of coatings using optical and scanning electron microscopy. Results of EDS analysis of the coating and hardness measurements indicated that a relatively high dilution (26.1 %) of the base material (BM) and the filler material (FM) occurred in a very narrow zone above the fusion line, and thus did not significantly affect the chemical composition of the rest of the coating. Also, erosion resistance tests of coatings were performed by changing the basic functional parameters that is the impact angle and the speed of erodent particles. It was observed that the erosion resistance of the coating material decreases approximately linearly with the increase of the particle speed. Also, with the increase of the impact angle of the erodent (up to 45 °), the mass loss of the coating material is increased.Delovi mašina i konstrukcija su tokom rada izloženi delovanju agresivne radne sredine koja u kratkom vremenskom periodu može da naruši njihov integritet. U zavisnosti od radnih uslova, površina materijala može da bude izložena raznim mehanizmima oštećenja, od kojih se izdvajaju eroziona i koroziona oštećenja. Produžetak radnog veka ugroženih zona mašinskih konstrukcija moguće je izvesti različitim postupcima nanošenja zaštitnih prevlaka, a najčešće su to različiti postupci navarivanja i metalizacije. Cilj ovog rada je karakterizacija prevlake izrađene od nerđajućeg čelika klase 316L, koja je našla široku primenu u hemijskoj i petrohemijskoj industriji, nanete plazma postupkom navarivanja korišćenjem dodatnog materijala u obliku praha, na konstrukcioni čelik S235JR. U radu je izvršeno merenje tvrdoće u karakterističnim zonama prevlake i osnovnog materijala, kao i mikrostrukturna karakterizacija prevlake na optičkom i skenirajućem elektronskom mikroskopu. Takođe, izvršeno je eroziono ispitivanje prevlake promenom parametara ispitivanja, pre svega ugla nastrujavanja i brzine čestica erodenta

MRI in the evaluation of breast cancer patient response to neoadjuvant chemotherapy: predictive factors for breast conservative surgery

PURPOSEWe aimed to prospectively assess the role of dynamic contrast-enhanced magnetic resonance imaging (DCE-MRI) in the evaluation of predictive factors for breast conservative surgery during neoadjuvant chemotherapy.MATERIALS AND METHODSSixty-six patients were evaluated before the first treatment cycle, after the second cycle, and upon the completion of neoadjuvant chemotherapy according to largest tumor diameter, tumor volume, postcontrast enhancement, and tumor regression pattern. The patients were divided into responders (pathologic complete and near complete response) and nonresponders. Each subgroup was re-evaluated according to morphokinetic criteria for identification of candidates for breast conservative surgery.RESULTSIn responders (n=27), the lesion size upon the completion of neoadjuvant chemotherapy was significantly smaller compared to nonresponders (1.5±0.6 vs. 3.2±0.9 cm; P < 0.001), as was the volume (1.2 vs. 11.0 cm3; P < 0.001). The measured lesion size did not differ from the histologic size (1.5±0.6 vs. 1.2±0.6 cm; P = 0.09) and had a high correlation (r=0.93). In responders, the following parameters were significantly different before and after neoadjuvant chemotherapy: size (3.6±1.4 to 1.5±0.6 cm; P < 0.001), volume (17.6 to 1.2 cm3; P < 0.001), predominant concentric regression, plateau and continuous time-intensity curves (P < 0.001). DCE-MRI has the sensitivity of 87% and the accuracy of 77% to identify candidates for breast conservative surgery.CONCLUSIONSelected morphokinetic DCE-MRI parameters may contribute to the multidisciplinary decision when considering the selection of candidates for breast conservative surgery

Negative attitudes related to violence against women: gender and ethnic differences among youth living in Serbia

OBJECTIVES: This study aimed to identify to what extent negative attitudes towards intimate partner violence against women are present among young women and men living in Serbia, in Roma and non-Roma settlements. METHODS: We used the data from the 2010 Multiple Indicator Cluster Survey conducted in Serbia, for the respondents who were 15-24 years old. Regression analyses were used to examine the association between judgmental attitudes, socio-demographic factors and life satisfaction. RESULTS: In Roma settlements, 34.8% of men and 23.6% of women believed that under certain circumstances men are justified to be violent towards wives, while among non-Roma it was 5.6 and 4.0%, respectively. These negative attitudes were significantly associated with lower educational level, lower socio-economic status and being married. In multivariate model, in both Roma and non-Roma population women who were not married were less judgmental, while the richest Roma men were least judgmental (OR 0.40, 95% CI 0.18-0.87). CONCLUSIONS: Violence prevention activities have to be focused on promoting gender equality among youth in vulnerable population groups such as Roma, especially through social support, strengthening their education and employment

Discutindo a educação ambiental no cotidiano escolar: desenvolvimento de projetos na escola formação inicial e continuada de professores

A presente pesquisa buscou discutir como a Educação Ambiental (EA) vem sendo trabalhada, no Ensino Fundamental e como os docentes desta escola compreendem e vem inserindo a EA no cotidiano escolar., em uma escola estadual do município de Tangará da Serra/MT, Brasil. Para tanto, realizou-se entrevistas com os professores que fazem parte de um projeto interdisciplinar de EA na escola pesquisada. Verificou-se que o projeto da escola não vem conseguindo alcançar os objetivos propostos por: desconhecimento do mesmo, pelos professores; formação deficiente dos professores, não entendimento da EA como processo de ensino-aprendizagem, falta de recursos didáticos, planejamento inadequado das atividades. A partir dessa constatação, procurou-se debater a impossibilidade de tratar do tema fora do trabalho interdisciplinar, bem como, e principalmente, a importância de um estudo mais aprofundado de EA, vinculando teoria e prática, tanto na formação docente, como em projetos escolares, a fim de fugir do tradicional vínculo “EA e ecologia, lixo e horta”.Facultad de Humanidades y Ciencias de la Educació

Some consequences of repeated casting of Co-Cr dental alloy

The effect of multiple recasting of commercial Co-Cr base dental alloy (Dentalite-C) on its structural characteristics and ion release rates in artificial saliva has been investigated. The number of recasting was once, four and eight times. The as-cast microstructure became uniformer as the number of recasting was increased whereby macro-and micro-hardness and electrical conductivity of the castings decreased. Metallic ion release in artificial saliva was monitored in vitro over the period of 22 weeks at the temperature of 37 degrees C and pH 2.3. Only Co and Fe were evidenced in artificial saliva and their release rates are measured. The ion release rates increases with time. The ion release rate of Fe was larger than Co. The ion release rates increases substantially by increasing the number of recasting