Forage Yield and Soil Moisture Content in \u3cem\u3ePanicum Maximum\u3c/em\u3e cv. Tanzania Monoculture and in a Mixture with \u3cem\u3eLeucaena Leucocephala\u3c/em\u3e with Different Densities in Mexico

Cattle production is limited by forage availability during the dry season since water and soil fertility are the main factors limiting production. Leucaena leucocephala has good nutritive value (24-30% CP). It can stand drought and grazing and so its introduction into pastures is recommended as an alternative to forage production during the dry season. The aim of this study was to assess the effect of the introduction of L. leucocephala with different densities on biomass production of P. maximum and soil water content

Intake and Yield of \u3ci\u3eCynodon nlemfuensis\u3c/i\u3e Alone and Associated with \u3ci\u3eLeucaena leucocephala\u3c/i\u3e Grazed by Sheep

The objective of the present study was to determine dry matter intake (DMI) and the forage yield of green dry matter (GDM) of star grass (Cynodon nlemfuensis) alone and associated with Leucaena leucocephala cv. Perú grazed by sheep. The experimental treatments were: Systems T1) Star grass alone, and T2) Star grass + L. leucocephala. These treatments were evaluated during three seasons (i.e. Late-rainy, Dry and Rainy seasons). The availability of forage was lower (P \u3c 0.0001) in T1, 2543 kg GDM/ha, than with T2, 3092 kg GDM/ha. Green dry matter in the rainy season was greater (P \u3c 0.0001), with 3910 kg GDM/ha, than in the late-rainy and dry seasons, 2492 and 2052 kg GDM/ha, respectively. DMI was 5.9 and 7.6 g DM/kg of LW 0.75/hour for the T1 and T2, respectively (P \u3c 0.0001). DMI during the rainy season was lower (P \u3c 0.01) than that of the dry and late-rainy seasons, 6.2, 6.4 and 7.5 g DM/kg LW0.75/hour, respectively. The association C. nlemfuensis-L. leucocephala increased the availability of forage and the voluntary intake

Root Density in \u3cem\u3ePanicum maximum\u3c/em\u3e cv. Tanzania Monoculture and in a Mixture with \u3cem\u3eLeucaena leucocephala\u3c/em\u3e with Different Densities in Mexico

In Yucatan cattle production is limited by forage availability during the dry season. L. leucocephala has good nutritive value (24 - 30% CP) and can stand drought and grazing, therefore its use in mixture with grasses is recommended. However, in association both species could compete for light, water and nutrients. The aim of this study was to assess the effect of introduction of L. leucocephala with different densities on root density of P. maximum

Effect of Defoliation Frequency on Forage Yield from Intensive Silvopastoral Systems Compared to a Monoculture Grassland

Livestock production in the tropical regions of the world faces serious constraints as a result of climate change. Monoculture based pastures require the use of large amounts of nitrogen fertilizers to sustain production throughout the year. In general terms, tropical grasses are of low quality and when consumed by ruminant species, contribute to the emission of greenhouse gases (methane, carbon dioxide and nitrous oxide) (Herrero et al. 2009; Place et al. 2009). The establishment of intensive silvopastoral systems (iSPS) with associated shrubs legumes and grasses can increase the yield and quality of forage as well as fixation and transfer of atmospheric nitrogen (N) (Murgueitio et al. 2011). Therefore, the costs of nitrogen fertilizers and the emissions of greenhouse gases under practical conditions can be reduced. The intensive silvopastoral system is a kind of agroforestal practice that it is environmentally friendly and at the same time improves productivity of livestock systems. However, several aspects of its management have not been fully evaluated. This is the case of the response to defoliation, which is an important management factor associated to the overall biomass productivity (Solorio 2005)

Urinary Excretion of Mimosine Derivatives by Cows with and without Experience in Consumption of \u3cem\u3eLeucaena leucocephala\u3c/em\u3e

Leucaena leucocephala is a leguminous tree widely distributed in the tropical regions of the world. In Mexico, it has been incorporated into silvopastoral systems and is highly regarded, owing to its high content of crude protein. Nonetheless, L. leucocephala contains secondary metab-olites, such as mimosine, a non-protein free amino acid, which may induce toxic effects in unadapted ruminants that consume the forage (Hammond 1995). Although Synergistes jonesii, an anaerobic bacterium, has the ability to degrade 3,4-DHP and 2,3-DHP to non-toxic compounds (Allison et al. 1992), in Mexico its presence has not yet been confirmed. Recent work has suggested the occurrence of sub-clinical toxicity to 3,4-DHP and 2,3-DHP in cattle grazing L. leucocephala in Australia and Thailand (Graham 2007; Dalzell et al. 2012; Phaikaew et al. 2012). Several options such as the transfer of rumen liquor and the adaptation of ruminants to the intake of L. leucocephala have been studied in an attempt to reduce the excretion of mimosine and its metabolites (Palmer et al. 2010). The aim of the present work was to evaluate the effect of the experience of consumption of L. leucocephala on excretion of mimosine derivatives (3,4-DHP and 2,3-DHP) in the urine of cattle

Productive Performance of Growing Cattle Grazing a Silvopastoral System with \u3cem\u3eLeucaena leucocephala\u3c/em\u3e

In tropical regions, the feeding of cattle is usually based on the grazing of medium to low quality grasses. Low fertility of soils, changing climatic conditions and the poor management of pastures, have further reduced the quality and forage yield of pastures. The low availability and quality of grasses gives modest weight gains for grazing cattle and this in-turn causes low economical efficiency of cattle production systems (Campos et al. 2011). Silvopastoral systems represent a sustainable option for meat and milk production in the tropics. The association of grasses with legumes such as Leucaena leucocephala (leucaena) supply forage with high concentration of crude protein (Barros et al. 2012). There are reports in the scientific literature which show that intake of leucaena can result in good rates of growth in cattle (e.g. Shelton and Dalzell 2007); however the presence of the free amino acid mimosine and its metabolites (3,4-DHP and 2,3-DHP) in leucaena when the anaerobic bacteria Synergistes jonesii (Allison et al. 1992) is absent from the rumen, may induce subclinical toxicity in grazing ruminants (Graham 2007; Dalzell et al. 2012; Phaikaew et al. 2012). There are no reports in Mexico regarding the rate of growth of cattle grazing silvopastoral systems with leucaena. The aim of the present work was to evaluate the rate of growth of cattle grazing an association of Panicum maximum and leucaena compared to that of cattle fed a high grain ration (feedlot)

Urinary Excretion of Mimosine Metabolites by Hair Sheep Fed Foliage of \u3cem\u3eLeucaena leucocephala\u3c/em\u3e

Leucaena leucocephala is an adapted legume widely distributed in the tropical regions of Mexico. The high crude protein content of leucaena leaves renders it appropriate for ruminant feeding under commercial conditions. However, the foliage contains the non-protein amino acid mimosine, which, if consumed in high amounts, may induce toxicity in animals which have not previously consumed the legume or without microorganisms capable of degrading mimosine and its derivatives 2,3-DHP (dihydroxypyridine) and 3,4-DHP (Hammond 1995, Palmer et al. 2010, Dalzell et al. 2012). Barros-Rodríguez et al. (2012) found that dry matter intake and weight gain were reduced when sheep grazed paddocks with 55,000 plants of leucaena per hectare. Early work in Australia led to the isolation of Synergistes jonesii, an anaerobic bacterium able to degrade 3,4-DHP and 2,3-DHP to non-toxic compounds (Allison et al. 1992). In Mexico, the presence of this microorganism in the rumen has not yet been confirmed. Inoculation of non-accustomed animals with rumen liquor of ruminants adapted to the consumption of leucaena can reduce the impact of mimosine and its metabolites on animal health (Ghosh et al. 2009; Palmer et al. 2010). The aim of the present work was to evaluate the effects of transferring rumen liquor of cows adapted to the consumption of L. leucocephala to sheep without experience of consumption, on urinary excretion of 3.4-DHP and 2.3-DHP by means of a colorimetric technique

Rare predicted loss-of-function variants of type I IFN immunity genes are associated with life-threatening COVID-19

Background: We previously reported that impaired type I IFN activity, due to inborn errors of TLR3- and TLR7-dependent type I interferon (IFN) immunity or to autoantibodies against type I IFN, account for 15–20% of cases of life-threatening COVID-19 in unvaccinated patients. Therefore, the determinants of life-threatening COVID-19 remain to be identified in ~ 80% of cases. Methods: We report here a genome-wide rare variant burden association analysis in 3269 unvaccinated patients with life-threatening COVID-19, and 1373 unvaccinated SARS-CoV-2-infected individuals without pneumonia. Among the 928 patients tested for autoantibodies against type I IFN, a quarter (234) were positive and were excluded. Results: No gene reached genome-wide significance. Under a recessive model, the most significant gene with at-risk variants was TLR7, with an OR of 27.68 (95%CI 1.5–528.7, P = 1.1 × 10−4) for biochemically loss-of-function (bLOF) variants. We replicated the enrichment in rare predicted LOF (pLOF) variants at 13 influenza susceptibility loci involved in TLR3-dependent type I IFN immunity (OR = 3.70[95%CI 1.3–8.2], P = 2.1 × 10−4). This enrichment was further strengthened by (1) adding the recently reported TYK2 and TLR7 COVID-19 loci, particularly under a recessive model (OR = 19.65[95%CI 2.1–2635.4], P = 3.4 × 10−3), and (2) considering as pLOF branchpoint variants with potentially strong impacts on splicing among the 15 loci (OR = 4.40[9%CI 2.3–8.4], P = 7.7 × 10−8). Finally, the patients with pLOF/bLOF variants at these 15 loci were significantly younger (mean age [SD] = 43.3 [20.3] years) than the other patients (56.0 [17.3] years; P = 1.68 × 10−5). Conclusions: Rare variants of TLR3- and TLR7-dependent type I IFN immunity genes can underlie life-threatening COVID-19, particularly with recessive inheritance, in patients under 60 years old

Autoantibodies against type I IFNs in patients with life-threatening COVID-19

Interindividual clinical variability in the course of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is vast. We report that at least 101 of 987 patients with life-threatening coronavirus disease 2019 (COVID-19) pneumonia had neutralizing immunoglobulin G (IgG) autoantibodies (auto-Abs) against interferon-w (IFN-w) (13 patients), against the 13 types of IFN-a (36), or against both (52) at the onset of critical disease; a few also had auto-Abs against the other three type I IFNs. The auto-Abs neutralize the ability of the corresponding type I IFNs to block SARS-CoV-2 infection in vitro. These auto-Abs were not found in 663 individuals with asymptomatic or mild SARS-CoV-2 infection and were present in only 4 of 1227 healthy individuals. Patients with auto-Abs were aged 25 to 87 years and 95 of the 101 were men. A B cell autoimmune phenocopy of inborn errors of type I IFN immunity accounts for life-threatening COVID-19 pneumonia in at least 2.6% of women and 12.5% of men