Automated device for continuous stirring while sampling in liquid chromatography systems

Ultra-performance liquid chromatography is a common analysis tool, and stirring is common in many laboratory setups. Here we show a device which enables continuous stirring of samples whilst inside an ultra-performance liquid chromatography system. Utilizing standard magnetic stirring bars that fit standard vials, the device allows for the automation of experimental setups that require stirring. The device is designed such that it can replace the standard sample holder and fits in its place, while being battery operated. The use of three-dimensional (3D) printing and commercially available parts enables low-effort and low-cost device production, as well as easy modifications. Testing the device was performed by video analysis and by following the kinetics of a dynamic combinatorial library that is known to be exquisitely sensitive to agitation, as a result of involving a fiber growth-breakage mechanism. Design files and schematics are provided

Automated Stirring Device for Continuous Stirring While Sampling in Liquid Chromatography Systems

A device is presented, which enables continuous stirring of samples whilst inside an ultra-performance liquid chromatography system. Utilizing standard magnetic stirring bars that fit standard vials, the device allows for the automation of experimental setups that require stirring. The device is designed such that it can replace the standard sample holder and fits in its place, while being battery operated. The use of 3D printing and commercially available parts enables low-effort and low-cost device production, as well as easy modifications. Various tests were performed by following the kinetics of a dynamic combinatorial library that is known for exhibiting self-replication under mechanical agitation, via fiber growth-breakage mechanism. Design files and schematics are available.<br /

Accompanying files: Automated device for continuous stirring while sampling in liquid chromatography systems

3D STL files of the components required for reproducing the stirring device, as well as supplementary files. Full details are available at the full manuscript or from the corresponding author. Acknowledgements: O.M. is funded through the NWA StartImpuls. This work has been funded by the ERC (AdG 741774), the NWO (Vici grant 724.012.002) and the Dutch Ministry of Education, Culture and Science (Gravitation program 024.001.035). We thank Andreas Hussain for discussions