Expression of Sec61a in F9 and P19 teratocarcinoma cells after retinoic acid treatment

Nascent procollagen peptides and other secretory proteins are transported across the endoplasmic reticulum (RE) membrane through a protein-conducting channel called the translocon. Sec61alpha, a multispanning membrane translocon protein, has been implicated as essential for translocation of polypeptides chains into the cisterns of the ER. However, it is not known whether Sec61alpha is ubiquitously expressed in collagen producing teratocarcinoma cells. Furthermore, the production, expression, and utilization of Sec61alpha may depend on the cell differentiation stage. Stem cells from many cultured teratocarcinoma cell lines such as F9 and P19 cells are capable of differentiation in response to low retinoic acid concentrations. This differentiation of the tumorigenic stem cells results in tumorigenicity loss. For this study, mouse F9 and P19 teratocarcinoma cells were grown in culture medium treated with or without retinoic acid. Expression of Sec61alpha was determined by reverse trancriptase polimerase chain reaction (RT-PCR). In untreated conditions, F9 cells expressed undetected Sec61alpha amounts. It was also demonstrated that Sec61alpha expression is stimulated in F9 cells after retinoic acid treatment for 72 hours. No changes were found in Sec61alpha expression in P19 cells after retinoic acid treatment. These data indicate that the expression of Sec61alpha is enhanced with retinoic acid induced differentiation of F9 teratocarcinoma cells

Expression of Sec61alpha in F9 and P19 teratocarcinoma cells after retinoic acid treatment

Nascent procollagen peptides and other secretory proteins are transported across the endoplasmic reticulum (RE) membrane through a protein-conducting channel called the translocon. Sec61alpha, a multispanning membrane translocon protein, has been implicated as essential for translocation of polypeptides chains into the cisterns of the ER. However, it is not known whether Sec61alpha is ubiquitously expressed in collagen producing teratocarcinoma cells. Furthermore, the production, expression, and utilization of Sec61alpha may depend on the cell differentiation stage. Stem cells from many cultured teratocarcinoma cell lines such as F9 and P19 cells are capable of differentiation in response to low retinoic acid concentrations. This differentiation of the tumorigenic stem cells results in tumorigenicity loss. For this study, mouse F9 and P19 teratocarcinoma cells were grown in culture medium treated with or without retinoic acid. Expression of Sec61alpha was determined by reverse trancriptase polimerase chain reaction (RT-PCR). In untreated conditions, F9 cells expressed undetected Sec61alpha amounts. It was also demonstrated that Sec61alpha expression is stimulated in F9 cells after retinoic acid treatment for 72 hours. No changes were found in Sec61alpha expression in P19 cells after retinoic acid treatment. These data indicate that the expression of Sec61alpha is enhanced with retinoic acid induced differentiation of F9 teratocarcinoma cells

Expression of Sec61alpha in F9 and P19 teratocarcinoma cells after retinoic acid treatment

Nascent procollagen peptides and other secretory proteins are transported across the endoplasmic reticulum (RE) membrane through a protein-conducting channel called the translocon. Sec61alpha, a multispanning membrane translocon protein, has been implicated as essential for translocation of polypeptides chains into the cisterns of the ER. However, it is not known whether Sec61alpha is ubiquitously expressed in collagen producing teratocarcinoma cells. Furthermore, the production, expression, and utilization of Sec61alpha may depend on the cell differentiation stage. Stem cells from many cultured teratocarcinoma cell lines such as F9 and P19 cells are capable of differentiation in response to low retinoic acid concentrations. This differentiation of the tumorigenic stem cells results in tumorigenicity loss. For this study, mouse F9 and P19 teratocarcinoma cells were grown in culture medium treated with or without retinoic acid. Expression of Sec61alpha was determined by reverse trancriptase polimerase chain reaction (RT-PCR). In untreated conditions, F9 cells expressed undetected Sec61alpha amounts. It was also demonstrated that Sec61alpha expression is stimulated in F9 cells after retinoic acid treatment for 72 hours. No changes were found in Sec61alpha expression in P19 cells after retinoic acid treatment. These data indicate that the expression of Sec61alpha is enhanced with retinoic acid induced differentiation of F9 teratocarcinoma cells

Avaliação in vitro da atividade antibacteriana da água ozonizada frente ao Staphylococcus aureus In vitro evaluation of the antibacterial activity of ozonized water against Staphylococcus aureus

O Staphylococcus aureus faz parte da microbiota da pele, mucosas e nasofaringe de várias espécies animais, incluindo o homem, mas também está associado a enfermidades como abscessos, bacteremias, endocardites e osteomielites, além de se mostrar resistente a múltiplas drogas. Este trabalho foi realizado com a finalidade de avaliar o efeito do gás ozônio, dissolvido em água, sobre o S. aureus. Foram preparadas suspensões de S. aureus, com concentrações variando de 10(6) a 10(16) microrganismos/ml. De cada suspensão recém-preparada foi retirado 1 ml e adicionado a 99 ml de água destilada estéril (com ou sem prévia ozonização), em um reator de cristal. Foram preparadas diluições seriadas (1/10) das suspensões testadas e inoculado 0,1 ml de cada suspensão em Tryptic Soy Agar, incubadas a 37ºC por 24 h, quando então se procedeu à contagem das UFC. Os resultados obtidos mostraram que o tempo máximo para a inativação total das bactérias tratadas com água previamente ozonizada (0,6 mg/ml) foi de 5&#146;25" e, para a água não previamente ozonizada, foi de 23&#146;45", indicando um efeito antibacteriano mais rápido da água previamente ozonizada, frente ao S. aureus.<br>Staphylococcus aureus belongs to the normal flora of the skin, mucosa and nasopharynx of several animal species, including man, but it is also associated to illnesses such as abscesses, bacteremia, endocarditis and osteomyelitis, besides showing resistance to multiple drugs. The purpose of this paper was to evaluate the disinfecting ability of ozone when dissolved in water. Suspensions of Staphylococcus aureus with concentrations varying from 10(6) to 10(16) microorganisms/ml were prepared. One milliliter of each recently prepared suspension was added to 99 ml of distilled water (with or without previous ozonization) contained in a crystal reactor. Aliquots of 0.1 ml of this new suspension were taken at various time intervals and, then, serially diluted and inoculated on plaques. The data indicated that there was difference in the disinfecting effect when distilled water was used with and without previous ozonization