Paraffin–Peloid Formulations from Copahue: Processing, Characterization and Application

The Copahue Thermal Center, situated in Neuquén, Argentina, produces natural and matured peloids, which are employed in the prevention and treatment of various osteoarticular and dermatological disorders. The presence of sulfur as a constituent and its thermotherapeutic potential constitute the primary strengths of these peloids. Nevertheless, accessing Copahue is challenging due to its distance from densely populated centers and the snow cover during the winter months in the southern hemisphere. Therefore, in order to propose a material that can be utilized year-round in any location, a mudpack was obtained by combining medicinal paraffin with dehydrated Copahue peloids, with concentrations evaluated up to 10% w/w. This mudpack was analyzed through X-ray diffraction, which detected the presence of sulfur, the most important component of Copahue’s peloids. Through IR spectroscopy, the signals that identify medicinal paraffin were clearly observed, and for concentrations of 6% and 10% peloid in the material, it was possible to detect the presence of mineral clay components associated with Si-O stretching vibrations at around 1041 cm−1. The low values of luminosity and grey tonality obtained for the mudpack contributed to patient acceptability and the absorption of electromagnetic radiation. The experimental cooling rate, calculated using the ratio of the temperature variation (∆T) with respect to the time variation (∆t) in each interval of the experimental curve, was determined to be 0.6 °C·min−1 for both paraffin and the mudpack. However, for peloids, higher values ranging from 0.6 to 4.8 °C·min−1 were obtained. This suggests that the mudpack mixtures have a slower heat release, which is a desirable property for their use as a thermotherapeutic agent. Considering the reusability of the mudpack, its stability was evaluated after 10 cycles of cooling and heating through XRD, DSC, and FTIR tests, resulting in a system that retains its properties. The formulation of the obtained mudpack is promising for the development of these materials on a larger scaleThis research was supported by the National University of Comahue, through project 04–I249: “Materiales adsorbentes naturales y modificados aplicados en termalismo

Adding Semantics to Enrich Public Transport and Accessibility Data from the Web

Web technologies and open data practices have now begun to promote new issues and services addressed to both final and specialized users. The smart cities initiative has also introduced new trends and ideas to offer to the public, one of which is the challenge of a more inclusive society that will provide the same opportunities for all. One of the major areas that could benefit from these new initiatives is public transport by, for example, providing open and accessible datasets, which include information by and about people with special needs. In this sense, the Google Transit Feed Specification (GTFS) defines a format to describe public transportation and associated geographic information. It includes details regarding accessibility and what people with special needs might require to get around using public transport. We are, however, of the opinion that this specification has a low granularity and is not sufficient, since it only takes into account only mobility needs. As suggestions for improvement, we propose to enrich GTFS data by combining public transport data from multiple Web sources with semantic metadata techniques. Those data are stored in a public semantic dataset. To define this dataset, we propose a systematic method to extract data from different sources and integrate them. This method is applied to obtain data about the metro system from the website of Metro Madrid and GTFS. Relevant SPARQL queries and two applications are developed to evaluate the usefulness of the dataset obtained

Boundary region between coexisting lipid phases as initial binding sites for Escherichia coli alpha-hemolysin: A real-time study

Abstractα-Hemolysin (HlyA) is a protein toxin, a member of the pore-forming Repeat in Toxin (RTX) family, secreted by some pathogenic strands of Escherichia coli. The mechanism of action of this toxin seems to involve three stages that ultimately lead to cell lysis: binding, insertion, and oligomerization of the toxin within the membrane. Since the influence of phase segregation on HlyA binding and insertion in lipid membranes is not clearly understood, we explored at the meso- and nanoscale—both in situ and in real-time—the interaction of HlyA with lipid monolayers and bilayers. Our results demonstrate that HlyA could insert into monolayers of dioleoylphosphatidylcholine/sphingomyelin/cholesterol (DOPC/16:0SM/Cho) and DOPC/24:1SM/Cho. The time course for HlyA insertion was similar in both lipidic mixtures. HlyA insertion into DOPC/16:0SM/Cho monolayers, visualized by Brewster-angle microscopy (BAM), suggest an integration of the toxin into both the liquid-ordered and liquid-expanded phases. Atomic-force-microscopy imaging reported that phase boundaries favor the initial binding of the toxin, whereas after a longer time period the HlyA becomes localized into the liquid-disordered (Ld) phases of supported planar bilayers composed of DOPC/16:0SM/Cho. Our AFM images, however, showed that the HlyA interaction does not appear to match the general strategy described for other invasive proteins. We discuss these results in terms of the mechanism of action of HlyA

Consuming Web Data in a Guiding App for Public Bus Users

The complexity of urban public bus networks in big cities makes their use very difficult. This paper presents Notify.me, a set of pervasive services for mobility that employs open data from the public bus network in Madrid. Our solution provides both a guiding service to assist users travelling by bus and a notifying service (visual, acoustical and sensorial) that informs them when a relevant point on their route has been reached (transfer or destination). Notify.me needs a starting point, which can be the user's current location, a destination and the preferences regarding the best route for the user. Notify.me requests a route from the Madrid public bus company via SOAP Web services. The back-end responds with the calculated route, the user's route, which includes the bus lines, the transfers and the pedestrian routes needed to reach the destination. Finally, an empirical evaluation of the experiences of users who employed Notify.me is presented

Three-year study of antibody to Borrelia burgdorferi in southern Spain

The prevalence of anti-Borrelia burgdorferi antibodies was studied in Granada, Spain, between January 1991 and November 1993 in 354 patients with suspected Lyme disease (group 1); in 50 patients either with syphilis (n = 32) or without syphilis but with a positive Rapid Plasma Reagin test (n = 18) (group 2); and in 150 healthy subjects (group 3). In addition, intrathecal antibody production was evaluated by EIA in CSF samples obtained from 117 patients in group 1. Anti-Borrelia burgdorferi antibodies were detected by EIA in 58 patients (16.4%) in group 1, 29 (8.2%) of whom were positive by Western blot. Intrathecal antibody production was detected in one patient. In group 2, 8 (16%) patients had a positive EIA result, but none of these was confirmed by Western blot. western blot was negative for all subjects in group 3. The results of this study indicate that anti-Borrelia burgdorferi antibodies are not uncommon in our area, although Lyme disease is rare

The electrochemistry of nanostructured Ni–W alloys

This work reports on the features that Ni–W nanostructured alloys, electrodeposited on carbon steel by different current pulse programs, may present depending on their surface morphology and surface composition. The Ni–W nanostructured coating, with a cauliflower structure, lack of fragility, and high WO3/W surface composition ratio, is a stable electrode to catalyze hydrogen evolution reaction, exceeding bulk and electrodeposited Ni catalytic activity. Also, the nanostructured alloys must have a low WO3/W surface composition ratio for Ni and its oxides to provide protection and improve corrosion resistance in sulfate media.Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicada

Design and evaluation of synthetic bacterial consortia for optimized phenanthrene degradation through the integration of genomics and shotgun proteomics

Two synthetic bacterial consortia (SC) composed of bacterial strains Sphingobium sp. (AM), Klebsiella aerogenes (B), Pseudomonas sp. (Bc-h and T), Burkholderia sp. (Bk) and Inquilinus limosus (Inq) isolated from a natural phenanthrene (PHN)-degrading consortium (CON) were developed and evaluated as an alternative approach to PHN biodegradation in bioremediation processes. A metabolic network showing the potential role of strains was reconstructed by in silico study of the six genomes and classification of dioxygenase enzymes using RHObase and AromaDeg databases. Network analysis suggested that AM and Bk were responsible for PHN initial attack, while Inq, B, T and Bc-h would degrade PHN metabolites. The predicted roles were further confirmed by physiological, RT-qPCR and metaproteomic assays. SC-1 with AM as the sole PHN degrader was the most efficient. The ecological roles inferred in this study can be applied to optimize the design of bacterial consortia and tackle the biodegradation of complex environmental pollutants.EEA RafaelaFil: Macchi, Marianela. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Festa, Sabrina. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Nieto, Esteban. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales; ArgentinaFil: Irazoqui, Jose Matias. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Vega-Vela, Nelson E. Pontificia Universidad Javeriana; Colombia. Universidad de Bogotá Jorge Tadeo Lozano; ColombiaFil: Junca, Howard. Microbiomas Foundation. Division Ecogenomics & Holobionts. RG Microbial Ecology: Metabolism, Genomics & Evolution; ColombiaFil: Valacco, María Pía. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales. IQUIBICEN. Consejo Nacional de Investigaciones Científicas y Técnicas. IQUIBICEN; ArgentinaFil: Amadio, Ariel. Instituto Nacional de Tecnología Agropecuaria (INTA). Estación Experimental Agropecuaria Rafaela; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina.Fil: Morelli, Irma S. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentina. Comisión de Investigaciones Científicas de la Provincia de Buenos Aires; ArgentinaFil: Coppotelli, Bibiana M. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro de Investigación y Desarrollo en Fermentaciones Industriales. Universidad Nacional de La Plata. Centro de Investigación y Desarrollo en Fermentaciones Industriales; Argentin

Open Data Consumption Through the Generation of Disposable Web APIs

The ever-growing amount of information in today’s world has led to the publication of more and more open data, i.e., that which is available in a free and reusable manner, on the Web. Open data is considered highly valuable in situational scenarios, in which thematic data is required for a short life cycle by a small group of consumers with specific needs. In this context, data consumers (developers or data scientists) need mechanisms with which to easily assess whether the data is adequate for their purpose. SPARQL endpoints have become very useful for the consumption of open data, but we argue that its steep learning curve hampers open data reuse in situational scenarios. In order to overcome this pitfall, in this paper, we coin the term disposable Web APIs as an alternative mechanism for the consumption of open data in situational scenarios. Disposable Web APIs are created on-the-fly to be used temporarily by a user to consume open data. In this paper we specifically describe an approach with which to leverage semantic information from data sources so as to automatically generate easy-to-use disposable Web APIs that can be used to access open data in a situational scenario, thus avoiding the complexity and learning curve of SPARQL and the effort of manually processing the data. We have conducted several experiments to discover whether non-experienced users find it easier to use our disposable Web API or a SPARQL endpoint to access open data. The results of the experiments led us to conclude that, in a situational scenario, it is easier and faster to use the Web API than the corresponding SPARQL endpoint in order to consume open data.This work was supported in part by the Access@City coordinated Research Project through the Spanish Ministry of Science, Innovation and Universities under Grant TIN2016-78103-C2-1-R and Grant TIN2016-78103-C2-2-R; in part by the Plataforma intensiva en datos proveedora de servicios inteligentes de movilidad (MoviDA) Project through Rey Juan Carlos University; and in part by the Recolección y publicación de datos abiertos para la reactivación del sector turístico postCOVID-19 (UAPOSTCOVID19-10) Project through the Consejo Social of the University of Alicante. The work of César González-Mora was supported in part by the Generalitat Valenciana, and in part by the European Social Fund under Grant ACIF/2019/044

New lichen biota records from Santuario Histórico de Machu Picchu, Peru

Los líquenes (hongos liquenizados) son organismos que presentan una alta diversidad, especialmente en las regiones tropicales; sin embargo, en Perú su conocimiento aún es escaso. Utilizando una metodología convencional, así como literatura actualizada sobre la descripción de géneros y especies, se presenta una lista de los macro-líquenes presentes en el Santuario Histórico de Machu Picchu (Cusco, Perú). Se reportan 67 especies de macro-líquenes, 9 de los cuales son nuevos registros para el Perú. Las familias Parmeliaceae, Physiciaceae y Lobariaceae son las que presentan mayor riqueza de especies en el área de estudio.While high species diversity of lichen - lichenized fungi - is reported especially in the tropics, the studies on these organisms are still scarce in Peru. Using conventional methodology and current literature on the description of genera and species, we reported macro-lichens collected from Historic Sanctuary of Machu Picchu (Cusco, Peru). Sixty seven species of macro-lichens are reported to SHMP, nine new records to Peru are documented. Parmeliaceae, Physciaceae and Lobariaceae are the families with major number of species

Self-Assembled Monolayers on C(0001)

This chapter describes the application of tunneling and AFM to the study of inorganic and organic adsorbates on C(0001) at the submonolayer and ML level. The C(0001) surface can be taken as a model system for the study of adsorption processes because it is atomically smooth and exhibits a low chemical reactivity, allowing an easy handling in the atmosphere. The knowledge of adsorption on carbon is important in the field of electrocatalysis because carbon is widely used as a matrix for the dispersion of catalytically active metallic clusters.Instituto de Investigaciones Fisicoquímicas Teóricas y Aplicada