269 research outputs found
Ionic behavior assessment of surface-active compounds from corn steep liquor by exchange resins
Depending on their ionic nature, biosurfactants can be classified as nonionic, anionic, cationic, or amphoteric. The ionic behavior of biosurfactants is an important characteristic that dictates their use in industrial applications. In this work, a biosurfactant extract obtained from corn steep liquor was subjected to anionic or cationic resins, in order to study the ionic behavior under different operational conditions using response surface methodology. The independent variables included in the study are the dilution of biosurfactant solution, the amount of cationic or anionic resin, and the extraction time, whereas the dependent variables studied consisted of the surface tension of biosurfactant aqueous solution, after contacting with anionic or cationic resin. The results showed that biosurfactant extracted from corn steep liquor is amphoteric, since both resins were able to entrap this biosurfactant, making it particularly suited for use in personal care preparations for sensitive skin.Peer ReviewedPostprint (author's final draft
Characterization of ionic nature of biosurfactant extracted from corn steep liquor using ionic exchange resins
[Excerpt] Resins have been used for years for the purification and characterization of ionic substances. The use of these resins allows not only remove impurities or increase the concentration of metabolites from industrial streams, but also allows define their ionic structure. In general, surfactants can be classified as non-ionic, anionic, cationic or amphoteric, what is going to define their future application at industrial scale. For example, anionic surfactants present the greatest wetting and emulsifying properties, while cationic ones have excellent antibacterial properties as well as good emulsifier capacities. Otherwise, amphoteric surfactants, which may have anionic or cationic properties depending on pH, are widely used in personal care products because of their less irritability to skin and eyes in comparison with other types of surfactants. [...]Spanish Ministry of Economy and Competitiveness (FEDER funds under the project CTM2015-68904)University of Vigo (Spain
Potential applications of biosurfactant extract obtained from corn steep liquor in hair formulations
[Excerpt] Biosurfactants (BS) have great advantages as an eco-friendly alternative to synthetic surfactants used in hair formulations. Human hair contains fatty acids (palmitic, palmitoleic, oleic and stearic acid) that prevent hair dryness and avoid the lower scalp hair density. These fatty acids are included in the composition of biosurfactant extract obtained from corn steep liquor (CSL) (Vecino et al., 2015). The adsorption of surfactants on hair depends on its ionic charge. Normally, hair surface has a negative charge, so it adsorbs cationic surfactants. For this reason, hair conditioners and also hair sunscreens contain cationic surfactants, mainly quaternary ammonium salts, which absorb UV light, protecting hair surface from dryness and oxidation. Contrarily, shampoo formulations are composed by anionic surface-active agents, which can induce, in many cases, hair protein loss, hair dryness, opacity and difficulty of handling. In order to know if biosurfactant extract, obtained from CSL, could be adsorbed on hair, its ionic behavior was evaluated by using anionic and cationic resins using a solid/liquid ratio of 1:10 at room temperature. After that, adsorption experiments using human hair were established at room temperature with hair/biosurfactant solution ratio of 1:50. [...]The financial support from the Spanish Ministry of Economy and Competitiveness
(FEDER funds under the project CTM2015-68904) and L. Rodríguez-López is grateful
for her predoctoral fellowship supported by the University of Vigo (Spain)
Effects of Adult Müller Cells and Their Conditioned Media on the Survival of Stem Cell-Derived Retinal Ganglion Cells
Retinal neurons, particularly retinal ganglion cells (RGCs), are susceptible to the degenerative damage caused by different inherited conditions and environmental insults, leading to irreversible vision loss and, ultimately, blindness. Numerous strategies are being tested in different models of degeneration to restore vision and, in recent years, stem cell technologies have offered novel avenues to obtain donor cells for replacement therapies. To date, stem cell–based transplantation in the retina has been attempted as treatment for photoreceptor degeneration, but the same tools could potentially be applied to other retinal cell types, including RGCs. However, RGC-like cells are not an abundant cell type in stem cell–derived cultures and, often, these cells degenerate over time in vitro. To overcome this limitation, we have taken advantage of the neuroprotective properties of Müller glia (one of the main glial cell types in the retina) and we have examined whether Müller glia and the factors they secrete could promote RGC-like cell survival in organoid cultures. Accordingly, stem cell-derived RGC-like cells were co-cultured with adult Müller cells or Müller cell-conditioned media was added to the cultures. Remarkably, RGC-like cell survival was substantially enhanced in both culture conditions, and we also observed a significant increase in their neurite length. Interestingly, Atoh7, a transcription factor required for RGC development, was up-regulated in stem cell-derived organoids exposed to conditioned media, suggesting that Müller cells may also enhance the survival of retinal progenitors and/or postmitotic precursor cells. In conclusion, Müller cells and the factors they release promote organoid-derived RGC-like cell survival, neuritogenesis, and possibly neuronal maturation.This work was supported by National Institutes of Health Grant R01EY026942 to A.L.T., and by the National Institutes of Health T32 Vision Science Training grant 4T32EY015387 to A.M.M. We also benefit from the National Eye Institute Core Facilities grant P30 EY012576. ELKARTEK KK-2019/00086 to E.V., Research groups of the UPV/EHU (GIU 2018/50) to E.V., Movilidad de personal de investigación UPV/EHU to X.P. and Programa de perfeccionamiento de personal Investigador Doctor, Gobierno Vasco (POS_2019_1_0027) to X.P
Adsorption capacity evaluation of a lipopeptide biosurfactant on human hair
[Excerpt] Nowadays, surfactants are used in a wide variety of applications in several fields, such as cosmetic and health care, environmental issues and, generally, as detergents. They present valuable characteristics since they can decrease water surface tension and solubilize molecules that cannot be eliminated in an aqueous media in normal conditions. Due to their surface active nature, synthetic surfactants can result toxic in the media in which they are applied. These drawbacks can be overcome by using biosurfactants, since they result environmentally friendly because of their biodegradability and low toxicity. Therefore, it would be interesting to replace synthetic detergents by biosurfactants in cosmetic and personal care formulations. From this point of view, Vecino et al., [1] have extracted biosurfactants from an industrial stream of corn wet milling industry, consisting of a lipopeptide that could have important applications in the formulation of natural shampoos. This biosurfactant is able to reduce the surface tension of water in more than 30 units. Thus, the aim of this work was to study the adsorption of this biosurfactant in human hair. [...]Spanish Ministry of Economy and Competitiveness (FEDER funds under the project
CTM2015-68904University of Vigo (Spain
Essential oil-water emulsions containing a biosurfactant from Lactobacillus paracasei
[Excerpt] Surfactants play important roles in cosmetic products due to diverse properties including as emulsifier, foaming, wetting, detergency and solubilizer agents, among others. However, the market for beauty and personal care products based on natural ingredients has shown a relevant growth. In this sense, biosurfactants being non-toxic, biodegradable and ecofriendly natural compounds are suitable replacers of the chemical-based surfactants showing equal or better performances [1]. In addition, essential oils are natural ingredients extracted from plants that also are interesting ingredients for novel cosmetic formulations.The aim of this study was to formulate oil-water emulsions using a biosurfactant from Lactobacil/us paracasei in combination with several essential oils (wheat germ, almond, rosemary and jojoba oil). [...]Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding
of UID/BI0/04469/2013 unit, COMPETE 2020 (POCl-01-0145-FEDER-006684) and the project RECl/BBB-EBl/0179/2012 (FCOMP-01-0124-FEDER-027462
Antimicrobial study of biosurfactants from Lactobacillus strains against skin pathogens
[Excerpt] Human skin microflora is composed by resident1 temporarily resident and transient microbial species. Therefore, gram-positive bacteria from the genera Propionibacterium, Staphylococcus, Micrococcus, Corynebaderium and Acinetobacter are the majority microorganisms present in the resident microflora [1 ]. For instance, Staphylococcus epidermidis is the main constituent of skin microflora, which protects the human skin from infections; whereas Staphylococcus aureus is a common transient species, which causes skin infections [2]. Anti-bacterial preservatives such as triclosan, methylparaben or bronopol, among others, are currently used as ingredients in the skin and oral care cosmetic products. However, there is a growing demand for cosmetics free of synthetic preservatives [3]. In this sense, biosurfactants from lactic acid bacteria, which are generally recognized as safe by the American Food and Drug Administration, are natural compounds that exhibit antimicrobial activity and therefore, could be used as an alternative to the chemically synthetized preservatives. The aim of this study was to evaluate the antimicrobial activity of biosurfactants obtained from two different Lactobacillus strains (L. pentosus and L. paracasef) against skin pathogenic microorganisms. [...]Portuguese Foundation for Science and Technology (FCT) under the scope of the strategic funding of
UID/BI0/04469/2013 unit, COMPETE 2020 (POCl-01-0145-FEDER-006684) and the project
RECl/BBB-EBl/0179/2012 (FCOMP-01-0124-FEDER-027462
New approach on the bioconversion of vineyard pruning waste into surface-active compounds by Lactobacillus paracasei
Strategic funding of UID/BIO/04469/2013 unit and project ref RECI/BBB-EBI/0179/2012 (project number FCOMP-01-0124-FEDER-027462)
and Xanel Vecino post-doctoral grant (ref SFRH/BPD/101476/2014) funded by Fundação para a Ciência e a Tecnologia, Portuga
Potential applications of multifunctional extract obtained from corn industry in food packaging
[Excerpt] Nowadays, in the food industry exists an increased demand, by the consumers, of natural additives that can replace those chemical additives obtained from non renewable resources. In this work extracts with surfactant and antioxidant properties were obtained from corn steep liquor (CSl) with potential applications in food packaging. the extraction process was carried out using ethyl acetate and the operational conditions consisted of: CSl/ethyl acetate 1:3 (v/v), at 25°C during 45 min with agitation system at 150 rpm. the antioxidant activity of the extract was determined by the dPPh (2,2-diphenyl-1-picrylhydrazyl) radical scavenging method; whereas the surface active properties was evaluated by the wilhelmy plate method in a force tensiometer with a platinum plate (easy dyne k20, kruSS gmbh), at room temperature. Fatty acid composition of extract was analyzed by gas chromatography coupled to a mass spectrometer (Bruker Scion 451-gC). [...
Comparative study between biobased fatty acid extract obtained from corn steep liquor and "Tsubaki" extract
[Excerpt] "Tsubaki" is a Japanese camellia oil extract, which is rich in palmitic and linoleic (Omega-6) fatty acids, as well as contain numerous anti-aging polyphenol antioxidants. On the other hand, a bio-based surfactant composed by 64.2% of lipids and 21.9% of proteins can be extracted from corn steep liquor (CSL) following the methodology proposed by Vecino et al. (2015). The aim of this work was to compare some biochemical properties of "Tsubaki" fatty acid extract, included in high-end cosmetic formulations of different brans, and the biobased surfactant obtained from CSL, in terms of surface active capacity reduction as well as in terms of antioxidant activity and fatty acid composition. [...]The financial support from the Spanish Ministry of Economy and Competitiveness
(FEDER funds under the project CTM2015-68904) and L. Rodríguez-López is grateful
for her predoctoral fellowship supported by the University of Vigo (Spain)
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