Assessment of Drumstick Tree (M. deifera) Accessions for Genetic Diversity in the Southern guinea Region of Nigeria

An experiment was conducted to analyze the genetic diversity among 9 drumstick tree (Moringaoleifera) accessions in the Teaching and Research Farm of the University of Agriculture Makurdi. The experiment was laid out in a Randomized Complete Block Design (RCBD) replicated three times. Data were recorded on growth and yield characteristics before and after pruning. The result obtained showed that at 18 weeks after transplanting, accession UAM-NI had the tallest plants (3.63m) while UAM-BE had the shortest mean plant height (2.84m) under no pruning. Other parameters that showed significant differences were number of leaves per tree and stem diameter. Although accession UAM-OY recorded highest fresh (220.22g), dry (113.42g) and leaf powder (82.60g) weights, it was not significantly different from other accessions. However, at 18 weeks after pruning, there was a significant difference among the accessions with regard to leaf length. Although accession UAM-NA recorded highest fresh leaf weight (286.60g), dry leaf weight (90.67g) and leaf powder weight (85.60g), it was not statistically different from other accessions. For the pruned accessions, significant differences were recorded in leaf length, number of flowers/tree, days to podding and fifty percent podding, pod length, pod girth, pod weight, number of seeds/pod, number of seeds/tree and 100seed weight. The result also indicated that the pruned accessions recorded higher leaf yield than the unpruned. The result of the cluster analysis grouped the accessions into two clusters and an outlier both for the pruned and unpruned accessions irrespective of area of collection

Pulmonary Disease and Age at Immigration among Hispanics. Results from the Hispanic Community Health Study/Study of Latinos

Rationale: Asthma has been reported to be more prevalent among Hispanics of Puerto Rican heritage than among other Hispanics and among Hispanics born in the United States or who immigrated as children than among those who came as adults; however, direct comparisons across Hispanic groups are lacking

Expression of CD3-ζ on T-cells in primary cervical carcinoma and in metastasis-positive and -negative pelvic lymph nodes

Lymphocytic infiltrate is often present in cervical cancer lesions, possibly reflecting an ongoing, but ineffective, immune response to the tumour. Recently, evidence has accumulated for systemically impaired T-cell functions in cancer patients, associated with decreased expression of signal-transducing zeta (ζ) chain dimer molecules on circulating T-cells and NK-cells. Here, we report on the intralesional down-regulation of ζ chain expression on T-cells in cervical carcinoma. Paraffin-embedded or snap-frozen sections from 24 different cervical cancer specimens were studied. Paraffin-embedded tumour-positive (n = 7) and tumour-negative (n = 15) pelvic lymph nodes were also included in the study. Immunostaining was performed on consecutive sections with antibodies specific for CD3-ɛ or the CD3-associated ζ chain dimer. Antigen retrieval by sodium citrate/microwave treatment was essential for ζ staining of paraffin sections. The amount of ζ positive cells was quantitated and related to the number of CD3-ɛ+ cells in corresponding tumour areas. Of the 24 cervical cancer specimens studied, ζ chain dimer expression was reduced in seven cases and strongly reduced in the other 17 samples. In tonsil control sections, CD3-ɛ and CD3-ζ were always co-expressed in almost equal numbers. Also, both tumour-negative and -positive lymph nodes showed ζ chain expression which equalled that of CD3-ɛ expression. These data indicate that a decreased expression of signal-transducing ζ molecules on tumour-infiltrating T-cells is frequent in cervical cancer. The apparently unimpaired ζ chain expression within draining lymph nodes suggests that local tumour-derived factors at the primary site are instrumental in ζ chain down-regulation. © 1999 Cancer Research Campaig

State of the Art Review: Emerging Therapies: The Use of Insulin Sensitizers in the Treatment of Adolescents with Polycystic Ovary Syndrome (PCOS)

PCOS, a heterogeneous disorder characterized by cystic ovarian morphology, androgen excess, and/or irregular periods, emerges during or shortly after puberty. Peri- and post-pubertal obesity, insulin resistance and consequent hyperinsulinemia are highly prevalent co-morbidities of PCOS and promote an ongoing state of excess androgen. Given the relationship of insulin to androgen excess, reduction of insulin secretion and/or improvement of its action at target tissues offer the possibility of improving the physical stigmata of androgen excess by correction of the reproductive dysfunction and preventing metabolic derangements from becoming entrenched. While lifestyle changes that concentrate on behavioral, dietary and exercise regimens should be considered as first line therapy for weight reduction and normalization of insulin levels in adolescents with PCOS, several therapeutic options are available and in wide use, including oral contraceptives, metformin, thiazolidenediones and spironolactone. Overwhelmingly, the data on the safety and efficacy of these medications derive from the adult PCOS literature. Despite the paucity of randomized control trials to adequately evaluate these modalities in adolescents, their use, particularly that of metformin, has gained popularity in the pediatric endocrine community. In this article, we present an overview of the use of insulin sensitizing medications in PCOS and review both the adult and (where available) adolescent literature, focusing specifically on the use of metformin in both mono- and combination therapy

Fluorescein angiography for the detection of metastases of ovarian tumor in the abdominal cavity, a feasibility pilot

Background and Objectives: The growth and progression of ovarian tumor metastases at the peritoneal surfaces of the abdominal cavity are coupled with neovascularization. Newly formed tumor vessels show a more diffuse pattern and are more permeable for macromolecules than normal vessels. We investigated the possibility to detect (small) ovarian metastases in the abdominal cavity by means of fluorescein angiography. Study Design/Patients and Methods: Eighteen patients known with ovarian cancer or with suspicion for this disease received sodium fluorescein intravenously in different doses (0.4-1.6 ml of a 25% solution), whereafter fluorescence detection by laparoscope was carried out. The distribution pattern of fluorescein was gained from 0 to 120 min by pooling the data collected in different patients at various time intervals. Results: Three phases could be discriminated, that is, rapid filling of blood vessels after administration of fluorescein, diffuse extravasation of fluorescein into surrounding tissues, and clearance of fluorescein from vessels and surrounding tissue. Differences in accumulation of fluorescein could be visualized only after 1 hour or more. Fluorescence imaging more then 60 minutes after administration of fluorescein did not give additional information compared to the inspection using white light, which was confirmed by histology. These observations were dose-independent. Conclusions: The concentration gradient differences of fluorescein between healthy and pathological tissue in the abdominal cavity are too small to indicate tumor neovascularizatio