Digital Dialectic: Forging New Paths of Inquiry in the Humanities

Digital Dialectic empowers humanities education with technology that sparks deeper contextual understanding of cultural artifacts and illuminates the multicultural nature of the humanities. Frederico Vigil's fresco, Mundos de Mestizaje, allegorically depicts 3000 years of Hispanic history, focusing on cross-cultural exchange of ideas. NHCC and ARTS Lab will create an interactive software application allowing users to explore the fresco, and through educational information embedded in the imagery, discover the dynamic nature of the humanities and their connection to Hispanidad. The asset will deploy on 2 interactive platforms: a digital dome presentation and a web-based viewer. The immersive dome piece will allow widespread audiences to view the fresco at actual scale and dive into details with high-resolution magnification; it will be distributed nationally and internationally to museums with fulldome theaters. The web-based viewer will allow self-guided exploration of the fresco

Reduced viability of N. crassa ergosterol mutants on Vogel\u27s medium.

Neurospora crassa ergosterol mutants are unable to synthesize ergosterol, the prevalent sterol in most filamentous fungi. These mutants have pleiotropic phenotypes such as reduced mycelial growth, decreased production of conidia, acquired tolerance to the polyene antibiotic nystatin (M. Grindle 1973 Mol. Gen. Genet. 120:283-290) and increased sensitivity to the pea phytoalexin pisatin (K. G. Papavinasasundaram and D. P. Kasbekar 1993 J. Gen. Microbiol. 139:3035-3041). We hoped to use the erg-1 mutant as a means of isolating pisatin detoxifying genes from other fungi by functional complementation for tolerance to pisatin. However, we observed that standard methods used for N. crassa were not suitable for ergosterol mutants because of their low viability on Vogel\u27s minimal medium (H. J. Vogel 1964 American Naturalist 98:435-446)

Table Facilitators' Reflections Regarding their Interprofessional Core Competencies

Background: Providing students and practitioners opportunities to learn from other disciplines in a supportive environment has the potential to improve patient outcomes and practitioner job satisfaction. Purpose: The purpose of this study was to describe an annual Interprofessional Education Event offered in a university setting and explore participant views regarding their competencies based on the Interprofessional Education Collaborative’s four core competency domains: Values/ethics for interprofessional practice, roles/responsibilities, interprofessional communication, and teams and teamwork. Method: Twenty-six faculty and students participated in preparatory activities and served as table facilitators for a large case study event. After the session, twenty submitted survey responses reflecting on changes in their interprofessional competencies. Discussion: Table facilitators reported that their core competencies in all areas remained stable or improved as a result of their participation in the pre-planning stages and case study workshop. Participant comments indicated the importance of initiating interprofessional education during academic training and to continue it throughout an individual’s career. Future directions include pre-event competency assessments and longer-term follow-up with participants

Source Apportionment And Emission Rates Of Volatile Organic Compounds In The Bakken Shale Oil And Natural Gas Region

Unconventional oil and gas (UONG) production using horizontal drilling and hydraulic fracturing has increased exponentially over the past decade in the Bakken Shale region located in eastern Montana and western North Dakota. Regional air quality is jeopardized during the development of this resource due to the volatile organic compounds (VOCs) that are emitted during drilling stages. In this study, the National Park Service measured VOC mixing ratios during an intensive field campaign in the winter of 2013-2014 to help better understand the potential impacts of these emissions on federal lands. Emission ratios with the tracer compounds, ethyne and propane, derived from a binary mixing model implicate regional oil and natural gas (ONG) production as the source of elevated alkanes in the Bakken Shale when compared to combustion emissions and background mixing ratios. Emission fluxes of alkanes calculated using a mass balance approach were similar to those from other ONG production regions, while annual emission rates for the 28,000 km region were an order of magnitude higher than rates from smaller regions. Hydroxyl radical reactivity was estimated in order to predict future regional ozone production and showed that 20-40% of total hydroxyl radical reactivity was attributable to ONG emissions

Pisatin demethylation by fungal pathogens and nonpathogens of pea: Association with pisatin tolerance and virulence

Previous studies have indicated that detoxification of their hosts’ phytoalexins is a tolerance mechanism for some true fungi, but not the fungus-like Oomycota, and may be involved in determining the virulence of a pathogen. In the present study, the associations between demethylation of the pea phytoalexin pisatin, tolerance to pisatin, and viru­lence on pea were examined for 50 fungal isolates which represent 17 species of pathogens and nonpathogens of pea. All isolates of Pythium coloratum and P. irregulare failed to metabolize and were sensitive to pisatin, consistent with previous observations that members of the Oomycota generally lack the ability to metabolize and are sensitive to their hosts’ phytoalexins. Among true fungi tested, the ability to demethylate pisatin was common, regardless of whether the particular isolate was pathogenic on pea or not. However, when the rate of pisatin demethylation was compared to virulence, all but one of the moderate to highly virulent isolates rapidly demethylated pisatin. In addition, the more rapidly demethylating isolates were generally more tolerant of pisatin. These results suggest that a specialized en­zyme system for quickly detoxifying pisatin might be present in most pea pathogens. In previous studies a specific cy­tochrome P450 enzyme for demethylating pisatin was identified in the pea pathogen Nectria haematococca mating pop­ulation VI, and genes (PDA genes) encoding that enzyme have been cloned from this fungus. When DNA specific for these genes was used to probe genomic DNA from other fungi that demethylate pisatin, significant hybridization was detected with only one fungus, the pea pathogen Fusarium oxysporum f. sp. pisi. If the other pea pathogens possess a specific cytochrome P450 system for detoxification of pisatin, the genes encoding these enzymes apparently share lim­ited nucleotide similarity with N. haematococca PDA genes

Pisatin demethylation by fungal pathogens and nonpathogens of pea: Association with pisatin tolerance and virulence

Previous studies have indicated that detoxification of their hosts’ phytoalexins is a tolerance mechanism for some true fungi, but not the fungus-like Oomycota, and may be involved in determining the virulence of a pathogen. In the present study, the associations between demethylation of the pea phytoalexin pisatin, tolerance to pisatin, and viru­lence on pea were examined for 50 fungal isolates which represent 17 species of pathogens and nonpathogens of pea. All isolates of Pythium coloratum and P. irregulare failed to metabolize and were sensitive to pisatin, consistent with previous observations that members of the Oomycota generally lack the ability to metabolize and are sensitive to their hosts’ phytoalexins. Among true fungi tested, the ability to demethylate pisatin was common, regardless of whether the particular isolate was pathogenic on pea or not. However, when the rate of pisatin demethylation was compared to virulence, all but one of the moderate to highly virulent isolates rapidly demethylated pisatin. In addition, the more rapidly demethylating isolates were generally more tolerant of pisatin. These results suggest that a specialized en­zyme system for quickly detoxifying pisatin might be present in most pea pathogens. In previous studies a specific cy­tochrome P450 enzyme for demethylating pisatin was identified in the pea pathogen Nectria haematococca mating pop­ulation VI, and genes (PDA genes) encoding that enzyme have been cloned from this fungus. When DNA specific for these genes was used to probe genomic DNA from other fungi that demethylate pisatin, significant hybridization was detected with only one fungus, the pea pathogen Fusarium oxysporum f. sp. pisi. If the other pea pathogens possess a specific cytochrome P450 system for detoxification of pisatin, the genes encoding these enzymes apparently share lim­ited nucleotide similarity with N. haematococca PDA genes

The Implementation of Therapeutic Alliance in the Rehabilitation of an Elite Pediatric Athlete with Salter-Harris Fracture: A Case Report

# Background/Purpose Although research on the value of therapeutic alliance is prominent in other areas of health care, physical therapy research is limited. The purpose is to describe the incorporation of therapeutic alliance concepts throughout the rehabilitation of an elite pediatric athlete with a complicated recovery following a fracture to the distal femoral epiphysis. # Case Description A 14-year-old male was referred to physical therapy following an open reduction and internal fixation to address a type IV Salter-Harris fracture of the right distal femoral epiphysis. Post-operative care included immobilization in a brace for six weeks and he initiated physical therapy for four weeks (post-op weeks 6-10). At 10-weeks post-injury his range of motion and strength were severely limited compared to expected post-operative milestones. Due to these deficits an arthroscopic debridement of the subject’s right knee, hardware removal, and manipulation under anesthesia was performed. The subject then reported to the physical therapist on post-operative day three for evaluation and treatment without bracing or weight-bearing restrictions. # Outcomes The episode of care spanned 17 weeks and included 25 physical therapy sessions. To facilitate therapeutic alliance with the subject, clear communication and easily measurable goals were established and connected to the subject’s relevant needs as an athlete. The plan of care was divided into three phases using “chunking” techniques to establish the rehabilitation priorities. The subject demonstrated improved range of motion, strength and was able to return to hydroplane racing and won a national championship in his age group. # Discussion The unique aspect of this case was the incorporation of therapeutic alliance concepts and techniques into the rehabilitative management of a subject with a complicated fracture to the distal femoral epiphysis. The physical therapist built trust with the subject and facilitated a successful return to elite hydroplane boat racing. # Level of Evidence 4 # Study Design Case Repor

Fermi-LAT Constraints on the Pulsar Wind Nebula Nature of HESS J1857+026

Since its launch, the Fermi satellite has firmly identified 5 pulsar wind nebulae plus a large number of candidates, all powered by young and energetic pulsars. HESS J1857+026 is a spatially extended gamma-ray source detected by H.E.S.S. and classified as a possible pulsar wind nebula candidate powered by PSR J1856+0245. Aims. We search for -ray pulsations from PSR J1856+0245 and explore the characteristics of its associated pulsar wind nebula. Methods. Using a rotational ephemeris obtained from the Lovell telescope at Jodrell Bank Observatory at 1.5 GHz, we phase.fold 36 months of gamma-ray data acquired by the Large Area Telescope (LAT) aboard Fermi. We also perform a complete gamma-ray spectral and morphological analysis. Results. No pulsation was detected from PSR J1856+0245. However, significant emission is detected at a position coincident with the TeV source HESS J1857+026. The gamma-ray spectrum is well described by a simple power law with a spectral index of Gamma = 1.53 +/- 0.11(sub stat) +/- 0.55(sub syst) and an energy flux of G(0.1 C100 GeV) = (2.71 +/- 0.52(sub stat) +/- 1.51(sub syst) X 10(exp -11) ergs/ sq cm/s. This implies a gamma.ray efficiency of approx 5 %, assuming a distance of 9 kpc, the gamma-ray luminosity of L(sub gamma) (sub PWN) (0.1 C100 GeV) = (2.5 +/- 0.5(sub stat) +/- 1.5(sub syst)) X 10(exp 35)(d/(9kpc))(exp 2) ergs/s and E-dot = 4.6 X 10(exp 36) erg /s, in the range expected for pulsar wind nebulae. Detailed multi-wavelength modeling provides new constraints on its pulsar wind nebula nature